Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 238028002

GI number: 238028002

Start: 2781871

End: 2783154

Strand: Reverse

Name: eno [H]

Synonym: bglu_1g24510

Alternate gene names: 238028002

Gene position: 2783154-2781871 (Counterclockwise)

Preceding gene: 238028003

Following gene: 238028001

Centisome position: 71.24

GC content: 64.64

Gene sequence:

>1284_bases
ATGAGTGCAATCGTAGATATCATCGGCCGCGAGATTCTGGATTCGCGCGGTAATCCTACTGTCGAGTGCGACGTGCTGCT
GGAATCGGGCACGATGGGCCGCGCGGCGGTCCCGTCGGGCGCCTCGACCGGTTCGCGCGAAGCCATCGAGCTGCGCGACG
GTGAAGCCGGCCGCTACGGCGGCAAGGGCGTGTTGAAGGCGGTCGAGCACATCAACACGGAAATCTCCGAAGCGATCATG
GGCCTCGATGCGTCGGAGCAGGCCTTCCTCGACAAGACCCTGCTCGAGCTCGACGGCACCGACAACAAGTCGCGGCTCGG
CGCGAACGCGATGCTGGCCGTCTCGATGGCCGTCGCGAAGGCCGCCGCCGAAGAGGCCGGCCTGCCGCTGTACCGCTACT
TCGGCGGCTCGGGCGCGATGCAGCTGCCGGTGCCGATGATGAACATCGTCAACGGCGGCGCGCACGCCAACAACAGCCTG
GACATCCAGGAATTCATGATCGTCCCGGTCAGCCAGCCGAGCTTCCGCGAGGCGCTGCGCTGCGGCGCCGAGGTGTTCCA
TGCGCTGAAGAAGATCCTGTCGGATCGCGGCATGAGCACGGCGGTCGGCGACGAAGGCGGCTTCGCACCGAACTTCGGCA
GCAACGACGAGTGCCTGTCGACGATTCTCCAGGCGATCGAGAAGGCCGGCTACCGCGCCGGCGAGGACGTGCTGCTCGCG
CTCGACTGCGCGGCCTCCGAGTTCTATCACGACGGCAAGTACCAGCTCGCGGGCGAGGGCCTGCAACTGTCGTCGGCCGA
ATTCGCCGACTATCTGGCCACGCTGGCCGACAAGTTCCCGATCGTCTCGATCGAGGACGGCATGCACGAAAGCGACTGGG
ACGGCTGGAAGCTGCTGACCGAGCGTCTCGGCAAGAAGGTCCAGCTGGTCGGCGACGACCTGTTCGTCACCAACACGCGA
ATCCTGAAGGAGGGCATCGAGAAGGGCATCGCCAATTCGATCCTCATCAAGATCAACCAGATCGGCACGCTCACGGAAAC
CTTCGCGGCGATCGAGATGGCCAAGCGTGCCGGCTACACGGCCGTGATTTCGCACCGCTCGGGCGAAACGGAAGATTCGA
CGATCGCCGACATCGCCGTGGGCCTGAACGCTGGCCAGATCAAGACCGGCTCGCTGTCGCGCAGCGACCGCATCTCGAAG
TACAACCAGCTGCTGCGCATCGAGGAAGATCTCGGTGACATCGCCAGCTACCCGGGCAAGTCGGCGTTCTACAATCTGCG
CTAA

Upstream 100 bases:

>100_bases
TAATGCATGAACTTGGCGGCAGGAGCGGGCGTCTGTAGCAGAGTCCGCTTTGCCGTCACAGCCATCACGACGGAATTCAT
CGTCATCCTCAGAGGAAATC

Downstream 100 bases:

>100_bases
CGCGCTACTATCCGGACTTGTCATCCTTGTCGCCGCTTCGCGTTTGCCGTGAAGCGGCGCTTCTTATATCTGCGGCTCGT
AAATGCGGCTCGTCACTGTC

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPSFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFADYLATLADKFPIVSIEDGMHESDWDGWKLLTERLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR

Sequences:

>Translated_427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPSFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFADYLATLADKFPIVSIEDGMHESDWDGWKLLTERLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR
>Mature_426_residues
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIMG
LDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLD
IQEFMIVPVSQPSFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLAL
DCAASEFYHDGKYQLAGEGLQLSSAEFADYLATLADKFPIVSIEDGMHESDWDGWKLLTERLGKKVQLVGDDLFVTNTRI
LKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKY
NQLLRIEEDLGDIASYPGKSAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=51.9450800915332, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.6519721577726, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=49.4279176201373, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=49.4279176201373, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=44.8598130841121, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.4880952380952, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.4880952380952, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.4880952380952, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=65.8823529411765, Blast_Score=558, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=49.6567505720824, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=49.6567505720824, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=44.1624365482233, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=46.3133640552995, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.6851851851852, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.6851851851852, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.6851851851852, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=47.0046082949309, Blast_Score=356, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=50.2336448598131, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=50.2336448598131, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=50.2336448598131, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=50.2336448598131, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=50.2336448598131, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=50.2336448598131, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45600; Mature: 45469

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFADYLATLADKFPIVSIEDGMHESDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ERLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC
>Mature Secondary Structure 
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYG
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFADYLATLADKFPIVSIEDGMHESDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ERLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA