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The map label for this gene is trmJ [H]

Identifier: 238027926

GI number: 238027926

Start: 2601030

End: 2601884

Strand: Reverse

Name: trmJ [H]

Synonym: bglu_1g23680

Alternate gene names: 238027926

Gene position: 2601884-2601030 (Counterclockwise)

Preceding gene: 238027928

Following gene: 238027925

Centisome position: 66.6

GC content: 72.4

Gene sequence:

>855_bases
TTGGCGATACCATACGGGCAATTCTTTCAGTCGGACCGAATCACGTTGGAAACCTCGCACCCCCCCTCGGCGCCCGCCGG
ACCCTCGTCCGTGCCGCCCCCGGCCGCAGGCGGCTTCGCCTCGGCGCGCTTCGTGCTGGTCGAGCCGAGCCATCCCGGCA
ACGTCGGCGCGGCGGCGCGCGCGCTCAAGACGATGGGTTTCTCGCGCCTCGTGCTGGTCGCGCCGCGCGTGGCCGGCGTG
CTGGCCGATCCCGAGGCGGTCGCGATGGCGAGCGGCGCCGACGACGTACTGGCCGCCACGCGCATCGTGCCGACGCTGGC
CGACGCGCTGGCGGGCGTGCAGTGGTCGGTCGCGCTGACCGCGCGCAGCCGCGAATACGGGCCGCCGCGCCTCGCACCGC
GTGCGGCCGCCGAGACGGCGCGCGCGCAGCTCGCCAGCGGCGAGATCGCGTTCGTGTTCGGCAATGAGCGCGTCGGGCTG
TCGAACGAGCACGTCGAGCGCTGCAGCGCGATCGCGCATATTCCGGCGAACCCCGAATACAGCTCGCTGAACCTGTCGCA
GGCGATCCAGGTGCTGGCCTACGAGCTGCGTGTCGCGCTGCTGTCGGGCAGCCCCGGCGGGCGTCACGACGGGGCGGCCG
GCACGCTGGCGCGGGCCGACGAGATCGAGGGGATGTTCGCGCACCTGGAGGCGGCACTCGTCGCACTCGATTTCCTCGAT
CCGCGCAACCCGAAGAAGCTGATGCCGCGGCTGCGGCGGATGTTCTCGCGCGCGGGGCTCGAGCGCGAGGAAGTCAACAT
CCTGCGCGGCATCGCCAAGCACATCGTGCTGAAGACGGGCGGCGGCGAGCGCTGA

Upstream 100 bases:

>100_bases
CCGAGTCGGGCCGTTTTTCCGAAATCGTGCCGTCGCGCCCCGAGCCGCCGCCATGCTCGCGCCCGCCCCGGCGGTGCGGC
CGGGTGGGATTGGCGCGGCG

Downstream 100 bases:

>100_bases
GCGGCGCCGGGGCCCGCCCGCGCCCCGGCCTGCCGCGCCAATACCCCCGGCGCCGTCGGGGGCAACGCGGTGGGTCCAGC
GCGTCCGCGCGAGTGGCCCT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MAIPYGQFFQSDRITLETSHPPSAPAGPSSVPPPAAGGFASARFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVAGV
LADPEAVAMASGADDVLAATRIVPTLADALAGVQWSVALTARSREYGPPRLAPRAAAETARAQLASGEIAFVFGNERVGL
SNEHVERCSAIAHIPANPEYSSLNLSQAIQVLAYELRVALLSGSPGGRHDGAAGTLARADEIEGMFAHLEAALVALDFLD
PRNPKKLMPRLRRMFSRAGLEREEVNILRGIAKHIVLKTGGGER

Sequences:

>Translated_284_residues
MAIPYGQFFQSDRITLETSHPPSAPAGPSSVPPPAAGGFASARFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVAGV
LADPEAVAMASGADDVLAATRIVPTLADALAGVQWSVALTARSREYGPPRLAPRAAAETARAQLASGEIAFVFGNERVGL
SNEHVERCSAIAHIPANPEYSSLNLSQAIQVLAYELRVALLSGSPGGRHDGAAGTLARADEIEGMFAHLEAALVALDFLD
PRNPKKLMPRLRRMFSRAGLEREEVNILRGIAKHIVLKTGGGER
>Mature_283_residues
AIPYGQFFQSDRITLETSHPPSAPAGPSSVPPPAAGGFASARFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVAGVL
ADPEAVAMASGADDVLAATRIVPTLADALAGVQWSVALTARSREYGPPRLAPRAAAETARAQLASGEIAFVFGNERVGLS
NEHVERCSAIAHIPANPEYSSLNLSQAIQVLAYELRVALLSGSPGGRHDGAAGTLARADEIEGMFAHLEAALVALDFLDP
RNPKKLMPRLRRMFSRAGLEREEVNILRGIAKHIVLKTGGGER

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=44.3089430894309, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=37.9746835443038, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29801; Mature: 29670

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPYGQFFQSDRITLETSHPPSAPAGPSSVPPPAAGGFASARFVLVEPSHPGNVGAAAR
CCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHH
ALKTMGFSRLVLVAPRVAGVLADPEAVAMASGADDVLAATRIVPTLADALAGVQWSVALT
HHHHCCCHHEEEECHHHHHHHCCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEE
ARSREYGPPRLAPRAAAETARAQLASGEIAFVFGNERVGLSNEHVERCSAIAHIPANPEY
ECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHEECCCCCCC
SSLNLSQAIQVLAYELRVALLSGSPGGRHDGAAGTLARADEIEGMFAHLEAALVALDFLD
CCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PRNPKKLMPRLRRMFSRAGLEREEVNILRGIAKHIVLKTGGGER
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
AIPYGQFFQSDRITLETSHPPSAPAGPSSVPPPAAGGFASARFVLVEPSHPGNVGAAAR
CCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHH
ALKTMGFSRLVLVAPRVAGVLADPEAVAMASGADDVLAATRIVPTLADALAGVQWSVALT
HHHHCCCHHEEEECHHHHHHHCCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEE
ARSREYGPPRLAPRAAAETARAQLASGEIAFVFGNERVGLSNEHVERCSAIAHIPANPEY
ECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHEECCCCCCC
SSLNLSQAIQVLAYELRVALLSGSPGGRHDGAAGTLARADEIEGMFAHLEAALVALDFLD
CCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PRNPKKLMPRLRRMFSRAGLEREEVNILRGIAKHIVLKTGGGER
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA