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The map label for this gene is alkA [H]

Identifier: 238027919

GI number: 238027919

Start: 2594676

End: 2595587

Strand: Reverse

Name: alkA [H]

Synonym: bglu_1g23610

Alternate gene names: 238027919

Gene position: 2595587-2594676 (Counterclockwise)

Preceding gene: 238027920

Following gene: 238027918

Centisome position: 66.44

GC content: 70.94

Gene sequence:

>912_bases
ATGGCAACGGGCAGGAAAGTGCCGGCAAAGCGGGCGGCGCGCACGGCGACGGGCGCGTCGGCGGCCGCGAAGCGCGTGCC
GGCCAGCAAGGTCGCCGCAAAGCGTGCGGCCGGCCCCGCCGAGGGCGCCAACGGCGCCGCGCACAAGCCGCTCGCGCGCG
GCGCGGCGAAGCCCGCGCGCGCCGACGCGGCCGAGCGTCGCGAACTGGCGGTCGACGCCGCCGACAGCGGCGCGCGGCGC
GCGCGCGCGGCCGAGGCGGCCGTGCCGGTGCAGCTTGCCGATGCGGACGTGGTGGTGCGCCCGCCCTACTGGGACAAGGC
GTGCGCCGATCTCGTCAAGCGCGACCGGATTCTCAAGAAGCTGATCCCCAAGTTCGGTCCCGCGCATCTCGTCAAGCGCG
GCGATCCGTTCGTCACGCTCGCACGCTCGGTGGTCGGCCAGCAGATCTCGGTCGCCACGGCGCAGGCCGTTTGGGCCGCG
ATCGAGGCCGCGTGCCCGAAGCTCACGCCGCAGCAGGTGATCCGCCTCGGCCAGGAGAAGCTCGCCGCCTGCGGCCTGTC
GAAGCGCAAGTCCGATTACATTCTCGATCTTGCCCAGCATTTCGTGTCGGGCGCGCTGCACGTCGACAAATGGACCTCGA
TGGAAGACGAGGCCGTGATCGCCGAGCTCACGCAGATCCGCGGCATCGGCCGTTGGACCGCCGAGATGTTCCTGATCTTC
AATCTGTCGCGCCCGGACGTCCTGCCGCTCGACGACCTCGGCCTGATTCGCGCGATCAGCATCAACTACTTCAGCGGCGA
GCCCGTCACGCGCAGCGAGGCGCGCGAGGTCGCGGCGAACTGGGAGCCGTGGCGTACAGTCGCGACCTGGTACATGTGGC
GCAGCCTGGATCCCGAATCCGGTGATGCGTAA

Upstream 100 bases:

>100_bases
CGGTGTCGCGCTCGAAGACAAACCGGGCGGGTTGACCGAATGGCGTCGCGTTTGATCGCACGTCGTCCGTTCCGCTGATC
GACTCGTCGCAGGAGGCAGG

Downstream 100 bases:

>100_bases
CCTGTGCTGACTATCGATTTATTCGAGTCAATAGTTTTGACTCGATTTTGACGTGGGTGAGCGCGGTTAGAATACGCGCT
GCCGCACGACCTAGGATTCC

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MATGRKVPAKRAARTATGASAAAKRVPASKVAAKRAAGPAEGANGAAHKPLARGAAKPARADAAERRELAVDAADSGARR
ARAAEAAVPVQLADADVVVRPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDPFVTLARSVVGQQISVATAQAVWAA
IEAACPKLTPQQVIRLGQEKLAACGLSKRKSDYILDLAQHFVSGALHVDKWTSMEDEAVIAELTQIRGIGRWTAEMFLIF
NLSRPDVLPLDDLGLIRAISINYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPESGDA

Sequences:

>Translated_303_residues
MATGRKVPAKRAARTATGASAAAKRVPASKVAAKRAAGPAEGANGAAHKPLARGAAKPARADAAERRELAVDAADSGARR
ARAAEAAVPVQLADADVVVRPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDPFVTLARSVVGQQISVATAQAVWAA
IEAACPKLTPQQVIRLGQEKLAACGLSKRKSDYILDLAQHFVSGALHVDKWTSMEDEAVIAELTQIRGIGRWTAEMFLIF
NLSRPDVLPLDDLGLIRAISINYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPESGDA
>Mature_302_residues
ATGRKVPAKRAARTATGASAAAKRVPASKVAAKRAAGPAEGANGAAHKPLARGAAKPARADAAERRELAVDAADSGARRA
RAAEAAVPVQLADADVVVRPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDPFVTLARSVVGQQISVATAQAVWAAI
EAACPKLTPQQVIRLGQEKLAACGLSKRKSDYILDLAQHFVSGALHVDKWTSMEDEAVIAELTQIRGIGRWTAEMFLIFN
LSRPDVLPLDDLGLIRAISINYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPESGDA

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320990, Length=197, Percent_Identity=31.9796954314721, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 32581; Mature: 32449

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00037 MYB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGRKVPAKRAARTATGASAAAKRVPASKVAAKRAAGPAEGANGAAHKPLARGAAKPAR
CCCCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHH
ADAAERRELAVDAADSGARRARAAEAAVPVQLADADVVVRPPYWDKACADLVKRDRILKK
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHH
LIPKFGPAHLVKRGDPFVTLARSVVGQQISVATAQAVWAAIEAACPKLTPQQVIRLGQEK
HHHCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LAACGLSKRKSDYILDLAQHFVSGALHVDKWTSMEDEAVIAELTQIRGIGRWTAEMFLIF
HHHHCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCHHHHHHHHHHHHCCHHHHEEEEEEE
NLSRPDVLPLDDLGLIRAISINYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPES
ECCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GDA
CCC
>Mature Secondary Structure 
ATGRKVPAKRAARTATGASAAAKRVPASKVAAKRAAGPAEGANGAAHKPLARGAAKPAR
CCCCCCCHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHH
ADAAERRELAVDAADSGARRARAAEAAVPVQLADADVVVRPPYWDKACADLVKRDRILKK
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHH
LIPKFGPAHLVKRGDPFVTLARSVVGQQISVATAQAVWAAIEAACPKLTPQQVIRLGQEK
HHHCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LAACGLSKRKSDYILDLAQHFVSGALHVDKWTSMEDEAVIAELTQIRGIGRWTAEMFLIF
HHHHCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCHHHHHHHHHHHHCCHHHHEEEEEEE
NLSRPDVLPLDDLGLIRAISINYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPES
ECCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GDA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]