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The map label for this gene is fadD25 [H]

Identifier: 238027870

GI number: 238027870

Start: 2529795

End: 2532053

Strand: Reverse

Name: fadD25 [H]

Synonym: bglu_1g23030

Alternate gene names: 238027870

Gene position: 2532053-2529795 (Counterclockwise)

Preceding gene: 238027871

Following gene: 238027869

Centisome position: 64.82

GC content: 71.49

Gene sequence:

>2259_bases
ATGACGACGCATTCGAACCCGAACGTGTTTCAGATGGATGTCGAACCGACCTCCATCGTCGAGACGCTCTCGCATCACAC
CGAGCGAGACCCTCATCGTGTCGCGCTGACCATCCTGCGCGACGGCGAAGCGATCGAAAGTGAAAGCACTTTCGGCGATC
TGCATGCCGGCATGCTGCGCGTCGCGCGCGGGCTCGCGCAGCTGACCGAGCCGGGCGCGCGCGTGCTGCTGCTGCTGCCG
ACGAGCGCCGAGTTCGTCTGCGCGTTCTACGGGTGCCTGGCGGCCGGGCGCATCGCCGTGCCGGCCTATCATCCGCAGCA
GGTACGCAAGGTGGCGCAATGGGCGAAGCTGCAGGCGATCGCCGACAACTGCGGCGCCACGCTGATCGTCGCGCCGGCGA
CGTCGGTCGACACCCTCGAGACCCTGCGCGCGACGCAGCAGCTGTTCGCCGGCTGCACGCTCGTGACATACGAGGCGCTC
GTCGCGGCGGGCGAGCAGGGCGCGTCGGCGGGGCCGCGCATGCCGGCGCCGGACGAGGTGGCCTTCCTGCAATACACCTC
CGGATCGACGGGCACGCCGAAGGGCGTGATGATCACGCATGGCAACATCATCGACAATCAACGCTCGATCGCGCGGCTCA
TGGAGCACGACCGCGATACGCGCATGCTGTCGTGGCTGCCCCTGTATCACGACATGGGATTGTCGATGCCGCTGCAGCTG
GCGTCGATCGGGCTGTCGGTCGTGCTGATGTCGCCCGTCGCGTTTGTCCAGCAGCCGGGCCGATGGCTGCGCGCGATTGC
AGCGCACCGTGCGACCACCTCGGGCGGCCCGAATTTCGCTTACGCGCTTGCCGCGGCCGCGCTCGAGGCGGGCGACGGCG
CCGACCGGCTCGACCTGTCGAGCTGGCGCGTGGCGTTCTGCGGCGCGGAGCCGATCCATCGGCGGACCGTCGTCGATTTC
ATCGAGGCCGCCCGCCCGCACGGCTTCGCGCCCGGGTCGTTCTATCCCTGCTACGGGATGGCCGAGGCGACCCTGATCGT
GTCGGGCGTGGCGCGCGGCGAAGGTGCCCATTATCTGGACGTCGACAGCCTGAAGCTCGCTGCCGGAACCGCCGAGCCGG
CCCGGCCCGGCGACGCGAACCTGAAGAGCCTCGTTTCGTGCGGCGCCGCGGCGCCCGGCACCGAGATCCGGATCGCCGGC
CCCGACGGCCGCCCGGTGGCCAGCCCGAACGGGGTCGGCGAGATCTGGGTGCGCGGCGGCGCGATCGGCGCCGGCTACTA
CGGCAACCCGGAAGCGACCCAGGCGACCTTCGCCGCGCGTTTCGAGGCCGATCCGGACGCGCGCCCGTTCCTGCGCACCG
GCGATCTGGGCGCGCTCGTCGACGGCCGCCTGTACGTGACCGGCCGCGTGAAGGACATGCTGATCGTGCGCGGGCGCAAC
CTCTATCCGCAGGATATCGAGGCGTGCGTGCAGGACGCGGTGCCGGCGCTGCGCCGCGGCTGCGGTGCCGCCGTGTCGGT
GCAGGCCGACGGCGAAGAGAAGCTCGTGATCATCCAGGAAGTCGGCCGCACCGCGCGCCGGACGATGGATCTCGGCGCCA
CGCTGCGCGAGATGGTGGTGGCGGTCAGCGAGGAGTTCGGCGTCACGCCGCATGCGATCGTGCTGGTCGAACCGGCCACC
ATCGACAAGACCTCGAGCGGCAAGATCGCGCGCGCGCTGTGCCGCCGCGCGTATCTGCAAGGCGAACTGCGGGCGGTCGC
AGGCTGGACCGACGGCGCCTATGCCGGCGCTGCGCCGAGCCCCGCGGCCGCGCGGCCGGCCCCCGAGCCCGGCGTCGGCG
CGGACGCCATGCTGATCGAGCGCGAGATCGCGCAGCGGATCGTCCGGATCGTCGCCGAGCGGCTCAAGATCGCCGAGACG
CAGGTCTCGCGCACGGCGGCGTGGGCCGAGATGGGCTTCGATTCGATGAGCGCGCTGCAATTCTCGTTGAAGGTGCAGCA
CGAGCTCGGGCTGAGCTTCGATGCCGCCGTGCTGTGGGACTGCTCGAACGTCGAGCAGCTCGCCGCGCACCTGGCCCGCA
TGCAGGGCGCAGCCGATCTGCTGCGAGCGGGCGGGGCGCCCGCACCGGCGGCCAGGCAGGTCGAGGCCGCGCCGGCCGGC
GCGCAGGCGCAGGCGCAGGCGCCGCTTACCGCGGCGCAGCTCGCGGCGCTCTCCGAGGCGGATGCGCAAGCGCTGCTGCT
CAAAGCGCTGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGTGCGATCGCGGCCACGTTCCTGCGCGACGCGTGAGATCGCGCCCGTGCGCGAGCCGCCTGCGCGCAGCGGCTCGCACC
GACCTCATTCTGGACACACC

Downstream 100 bases:

>100_bases
ACCATTCCCCGGCAGTCTCGTGCCGCGAACGTCAGGAAGCTTACCCATGAACGAATCCAGTATGGCGTCCGCTGCGCCGC
CCATTTCCCTTACGCCCGTG

Product: beta-ketoacyl synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MTTHSNPNVFQMDVEPTSIVETLSHHTERDPHRVALTILRDGEAIESESTFGDLHAGMLRVARGLAQLTEPGARVLLLLP
TSAEFVCAFYGCLAAGRIAVPAYHPQQVRKVAQWAKLQAIADNCGATLIVAPATSVDTLETLRATQQLFAGCTLVTYEAL
VAAGEQGASAGPRMPAPDEVAFLQYTSGSTGTPKGVMITHGNIIDNQRSIARLMEHDRDTRMLSWLPLYHDMGLSMPLQL
ASIGLSVVLMSPVAFVQQPGRWLRAIAAHRATTSGGPNFAYALAAAALEAGDGADRLDLSSWRVAFCGAEPIHRRTVVDF
IEAARPHGFAPGSFYPCYGMAEATLIVSGVARGEGAHYLDVDSLKLAAGTAEPARPGDANLKSLVSCGAAAPGTEIRIAG
PDGRPVASPNGVGEIWVRGGAIGAGYYGNPEATQATFAARFEADPDARPFLRTGDLGALVDGRLYVTGRVKDMLIVRGRN
LYPQDIEACVQDAVPALRRGCGAAVSVQADGEEKLVIIQEVGRTARRTMDLGATLREMVVAVSEEFGVTPHAIVLVEPAT
IDKTSSGKIARALCRRAYLQGELRAVAGWTDGAYAGAAPSPAAARPAPEPGVGADAMLIEREIAQRIVRIVAERLKIAET
QVSRTAAWAEMGFDSMSALQFSLKVQHELGLSFDAAVLWDCSNVEQLAAHLARMQGAADLLRAGGAPAPAARQVEAAPAG
AQAQAQAPLTAAQLAALSEADAQALLLKALQR

Sequences:

>Translated_752_residues
MTTHSNPNVFQMDVEPTSIVETLSHHTERDPHRVALTILRDGEAIESESTFGDLHAGMLRVARGLAQLTEPGARVLLLLP
TSAEFVCAFYGCLAAGRIAVPAYHPQQVRKVAQWAKLQAIADNCGATLIVAPATSVDTLETLRATQQLFAGCTLVTYEAL
VAAGEQGASAGPRMPAPDEVAFLQYTSGSTGTPKGVMITHGNIIDNQRSIARLMEHDRDTRMLSWLPLYHDMGLSMPLQL
ASIGLSVVLMSPVAFVQQPGRWLRAIAAHRATTSGGPNFAYALAAAALEAGDGADRLDLSSWRVAFCGAEPIHRRTVVDF
IEAARPHGFAPGSFYPCYGMAEATLIVSGVARGEGAHYLDVDSLKLAAGTAEPARPGDANLKSLVSCGAAAPGTEIRIAG
PDGRPVASPNGVGEIWVRGGAIGAGYYGNPEATQATFAARFEADPDARPFLRTGDLGALVDGRLYVTGRVKDMLIVRGRN
LYPQDIEACVQDAVPALRRGCGAAVSVQADGEEKLVIIQEVGRTARRTMDLGATLREMVVAVSEEFGVTPHAIVLVEPAT
IDKTSSGKIARALCRRAYLQGELRAVAGWTDGAYAGAAPSPAAARPAPEPGVGADAMLIEREIAQRIVRIVAERLKIAET
QVSRTAAWAEMGFDSMSALQFSLKVQHELGLSFDAAVLWDCSNVEQLAAHLARMQGAADLLRAGGAPAPAARQVEAAPAG
AQAQAQAPLTAAQLAALSEADAQALLLKALQR
>Mature_751_residues
TTHSNPNVFQMDVEPTSIVETLSHHTERDPHRVALTILRDGEAIESESTFGDLHAGMLRVARGLAQLTEPGARVLLLLPT
SAEFVCAFYGCLAAGRIAVPAYHPQQVRKVAQWAKLQAIADNCGATLIVAPATSVDTLETLRATQQLFAGCTLVTYEALV
AAGEQGASAGPRMPAPDEVAFLQYTSGSTGTPKGVMITHGNIIDNQRSIARLMEHDRDTRMLSWLPLYHDMGLSMPLQLA
SIGLSVVLMSPVAFVQQPGRWLRAIAAHRATTSGGPNFAYALAAAALEAGDGADRLDLSSWRVAFCGAEPIHRRTVVDFI
EAARPHGFAPGSFYPCYGMAEATLIVSGVARGEGAHYLDVDSLKLAAGTAEPARPGDANLKSLVSCGAAAPGTEIRIAGP
DGRPVASPNGVGEIWVRGGAIGAGYYGNPEATQATFAARFEADPDARPFLRTGDLGALVDGRLYVTGRVKDMLIVRGRNL
YPQDIEACVQDAVPALRRGCGAAVSVQADGEEKLVIIQEVGRTARRTMDLGATLREMVVAVSEEFGVTPHAIVLVEPATI
DKTSSGKIARALCRRAYLQGELRAVAGWTDGAYAGAAPSPAAARPAPEPGVGADAMLIEREIAQRIVRIVAERLKIAETQ
VSRTAAWAEMGFDSMSALQFSLKVQHELGLSFDAAVLWDCSNVEQLAAHLARMQGAADLLRAGGAPAPAARQVEAAPAGA
QAQAQAPLTAAQLAALSEADAQALLLKALQR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=570, Percent_Identity=25.2631578947368, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI44888818, Length=588, Percent_Identity=23.6394557823129, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI225735625, Length=608, Percent_Identity=23.6842105263158, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI45827692, Length=608, Percent_Identity=23.6842105263158, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI225735629, Length=608, Percent_Identity=23.6842105263158, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI45827694, Length=573, Percent_Identity=24.0837696335079, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI225735627, Length=511, Percent_Identity=24.0704500978474, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827696, Length=511, Percent_Identity=24.0704500978474, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45827698, Length=481, Percent_Identity=24.5322245322245, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=484, Percent_Identity=24.1735537190083, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786810, Length=439, Percent_Identity=24.6013667425968, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193202679, Length=576, Percent_Identity=25, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=557, Percent_Identity=22.262118491921, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=481, Percent_Identity=24.7401247401247, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=584, Percent_Identity=23.2876712328767, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859661, Length=536, Percent_Identity=24.2537313432836, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654656, Length=475, Percent_Identity=23.1578947368421, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281365686, Length=334, Percent_Identity=25.748502994012, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356947, Length=209, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 79249; Mature: 79118

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHSNPNVFQMDVEPTSIVETLSHHTERDPHRVALTILRDGEAIESESTFGDLHAGMLR
CCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
VARGLAQLTEPGARVLLLLPTSAEFVCAFYGCLAAGRIAVPAYHPQQVRKVAQWAKLQAI
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHH
ADNCGATLIVAPATSVDTLETLRATQQLFAGCTLVTYEALVAAGEQGASAGPRMPAPDEV
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AFLQYTSGSTGTPKGVMITHGNIIDNQRSIARLMEHDRDTRMLSWLPLYHDMGLSMPLQL
EEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
ASIGLSVVLMSPVAFVQQPGRWLRAIAAHRATTSGGPNFAYALAAAALEAGDGADRLDLS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
SWRVAFCGAEPIHRRTVVDFIEAARPHGFAPGSFYPCYGMAEATLIVSGVARGEGAHYLD
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEEHHHCCCCCEEEE
VDSLKLAAGTAEPARPGDANLKSLVSCGAAAPGTEIRIAGPDGRPVASPNGVGEIWVRGG
CCCEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECC
AIGAGYYGNPEATQATFAARFEADPDARPFLRTGDLGALVDGRLYVTGRVKDMLIVRGRN
EEECCCCCCCCHHHHHEEEEECCCCCCCCCEEECCCCEEECCEEEEEECCEEEEEECCCC
LYPQDIEACVQDAVPALRRGCGAAVSVQADGEEKLVIIQEVGRTARRTMDLGATLREMVV
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHHHHHH
AVSEEFGVTPHAIVLVEPATIDKTSSGKIARALCRRAYLQGELRAVAGWTDGAYAGAAPS
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
PAAARPAPEPGVGADAMLIEREIAQRIVRIVAERLKIAETQVSRTAAWAEMGFDSMSALQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FSLKVQHELGLSFDAAVLWDCSNVEQLAAHLARMQGAADLLRAGGAPAPAARQVEAAPAG
EEEEEHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AQAQAQAPLTAAQLAALSEADAQALLLKALQR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTHSNPNVFQMDVEPTSIVETLSHHTERDPHRVALTILRDGEAIESESTFGDLHAGMLR
CCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
VARGLAQLTEPGARVLLLLPTSAEFVCAFYGCLAAGRIAVPAYHPQQVRKVAQWAKLQAI
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHH
ADNCGATLIVAPATSVDTLETLRATQQLFAGCTLVTYEALVAAGEQGASAGPRMPAPDEV
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCE
AFLQYTSGSTGTPKGVMITHGNIIDNQRSIARLMEHDRDTRMLSWLPLYHDMGLSMPLQL
EEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
ASIGLSVVLMSPVAFVQQPGRWLRAIAAHRATTSGGPNFAYALAAAALEAGDGADRLDLS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
SWRVAFCGAEPIHRRTVVDFIEAARPHGFAPGSFYPCYGMAEATLIVSGVARGEGAHYLD
CCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHEEEHHHCCCCCEEEE
VDSLKLAAGTAEPARPGDANLKSLVSCGAAAPGTEIRIAGPDGRPVASPNGVGEIWVRGG
CCCEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECC
AIGAGYYGNPEATQATFAARFEADPDARPFLRTGDLGALVDGRLYVTGRVKDMLIVRGRN
EEECCCCCCCCHHHHHEEEEECCCCCCCCCEEECCCCEEECCEEEEEECCEEEEEECCCC
LYPQDIEACVQDAVPALRRGCGAAVSVQADGEEKLVIIQEVGRTARRTMDLGATLREMVV
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHCHHHHHHHHHHHHHHHHHH
AVSEEFGVTPHAIVLVEPATIDKTSSGKIARALCRRAYLQGELRAVAGWTDGAYAGAAPS
HHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
PAAARPAPEPGVGADAMLIEREIAQRIVRIVAERLKIAETQVSRTAAWAEMGFDSMSALQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FSLKVQHELGLSFDAAVLWDCSNVEQLAAHLARMQGAADLLRAGGAPAPAARQVEAAPAG
EEEEEHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AQAQAQAPLTAAQLAALSEADAQALLLKALQR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]