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The map label for this gene is dosC [H]

Identifier: 238027866

GI number: 238027866

Start: 2512188

End: 2513309

Strand: Reverse

Name: dosC [H]

Synonym: bglu_1g22970

Alternate gene names: 238027866

Gene position: 2513309-2512188 (Counterclockwise)

Preceding gene: 238027867

Following gene: 238027865

Centisome position: 64.34

GC content: 70.86

Gene sequence:

>1122_bases
TTGGAAAGGCACATGCACGCGCTGCTCGACACGGTTCGCCACAACGAGCGTGTGCTGCAGCGGTTTCAGCAGATCGAACT
GCGGCTCGTGAGTGCCACCGATTTCGCGGTGTTTCACGACACGCTGGTCGAGGCGCTGGCGCAGGCCTTCGAACTGCCGT
TCGTGACCTTGTGGCTCAACGAGGACATCTTGCTCGTTCGCGAGATGCTCGATGCCGTGTCGCGCGCGAAGCCGGCCGCG
CAGGCGGTGCTGCGCGGCCGGCTCGACGCGCTCGCCGGCGCGCTGCCCGGCGAGCCCGGCCAGCCCTGGCTAGGCGCGGG
TGCCGAACTCGGCGAGACGGCTCTTGCCGTGCTCTACGGCGGCTCGGAAGTGCCCGCCAGCGTCGCCGTGCTGCCGCTGC
ATCAGGAGCACGGCGTCAACGGCTATCTGTGCCTAGGCAGTCGCGACCCCGAGCGTTTCAGCGTCGGCAAGGCGACGGAT
CTGCTGGAGCGCTTCGCGGCCTTCGTCAGCGCCGGGGTGGTCAACGTCGCGCATCGCGAGCAGGTGGCCCGCTACGGCAT
GACCGACATGCTCACGAACCTGCCGAATCGCCGCTATTTCGACAGCCGCATCCACGACGAGATGCAGCGCTCGGCGAGAG
CGAAGTCGCCGGTCGCCTGCCTGTTCATCGACGTCGACCACTTCAAGCGCGTCAACGATACCTACGGCCATGCCGTGGGT
GACCGCGCGCTTGCCGCGATCGGCGCCTGCATTCGCAAGACGGTGCGCCACGGCGATACGGTCGCGCGCTACGGCGGCGA
GGAATTCGTCGTGCTGGTGCGCGGCGATGCCGTCCACGCCTGCAGCGTCGCCGAGCGCGTGCGCGCGGCGGTGGCGGCGC
TGGTGGCCGCGGAGGTGCGGGGCGACCCGGTCGGCCTGACGGTGTCGATCGGCGTGGCGGTCGGCAACGTCGGCGAGATC
GCCGACGGCGAAGCCGGCGCCGCCACGCGGCTCGTGGAGCACGCCGACCAGGCGCTCTACGTCGCCAAGCACGGCGGCCG
CAATTGCGTGGTGCTGCACCGCGCGGCGCCGGAGCCCGCTTCCCCCTGCGCGCCGCAGGCGCCGGCCCTGCCGGCGCCTT
GA

Upstream 100 bases:

>100_bases
CTCGTCGCGACCGCGCCTGTCCCTTAAGAAAGCCGAAAACCTGTCGATATGTCTAGGATCACCCCCAAATGCGATATCGC
CAGTGATCGAGGATTCTTCG

Downstream 100 bases:

>100_bases
ACGCGGCGCCGCGCGCCGCTCGCGTCCCGGGCGGCGCCGACCGGGCGCCGGTGGCAGGCGCTGGATGCGCGCGCAACCGA
ATTTCCGCACCGACGAAGGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MERHMHALLDTVRHNERVLQRFQQIELRLVSATDFAVFHDTLVEALAQAFELPFVTLWLNEDILLVREMLDAVSRAKPAA
QAVLRGRLDALAGALPGEPGQPWLGAGAELGETALAVLYGGSEVPASVAVLPLHQEHGVNGYLCLGSRDPERFSVGKATD
LLERFAAFVSAGVVNVAHREQVARYGMTDMLTNLPNRRYFDSRIHDEMQRSARAKSPVACLFIDVDHFKRVNDTYGHAVG
DRALAAIGACIRKTVRHGDTVARYGGEEFVVLVRGDAVHACSVAERVRAAVAALVAAEVRGDPVGLTVSIGVAVGNVGEI
ADGEAGAATRLVEHADQALYVAKHGGRNCVVLHRAAPEPASPCAPQAPALPAP

Sequences:

>Translated_373_residues
MERHMHALLDTVRHNERVLQRFQQIELRLVSATDFAVFHDTLVEALAQAFELPFVTLWLNEDILLVREMLDAVSRAKPAA
QAVLRGRLDALAGALPGEPGQPWLGAGAELGETALAVLYGGSEVPASVAVLPLHQEHGVNGYLCLGSRDPERFSVGKATD
LLERFAAFVSAGVVNVAHREQVARYGMTDMLTNLPNRRYFDSRIHDEMQRSARAKSPVACLFIDVDHFKRVNDTYGHAVG
DRALAAIGACIRKTVRHGDTVARYGGEEFVVLVRGDAVHACSVAERVRAAVAALVAAEVRGDPVGLTVSIGVAVGNVGEI
ADGEAGAATRLVEHADQALYVAKHGGRNCVVLHRAAPEPASPCAPQAPALPAP
>Mature_373_residues
MERHMHALLDTVRHNERVLQRFQQIELRLVSATDFAVFHDTLVEALAQAFELPFVTLWLNEDILLVREMLDAVSRAKPAA
QAVLRGRLDALAGALPGEPGQPWLGAGAELGETALAVLYGGSEVPASVAVLPLHQEHGVNGYLCLGSRDPERFSVGKATD
LLERFAAFVSAGVVNVAHREQVARYGMTDMLTNLPNRRYFDSRIHDEMQRSARAKSPVACLFIDVDHFKRVNDTYGHAVG
DRALAAIGACIRKTVRHGDTVARYGGEEFVVLVRGDAVHACSVAERVRAAVAALVAAEVRGDPVGLTVSIGVAVGNVGEI
ADGEAGAATRLVEHADQALYVAKHGGRNCVVLHRAAPEPASPCAPQAPALPAP

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=40, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787262, Length=195, Percent_Identity=37.4358974358974, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=33.5260115606936, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=188, Percent_Identity=31.9148936170213, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=40.2298850574713, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787541, Length=214, Percent_Identity=29.4392523364486, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=32.5153374233129, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787802, Length=203, Percent_Identity=32.0197044334975, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787816, Length=166, Percent_Identity=34.3373493975904, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=34.1040462427746, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=235, Percent_Identity=27.2340425531915, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=31.4285714285714, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=109, Percent_Identity=32.1100917431193, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=32.5, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39934; Mature: 39934

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERHMHALLDTVRHNERVLQRFQQIELRLVSATDFAVFHDTLVEALAQAFELPFVTLWLN
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEC
EDILLVREMLDAVSRAKPAAQAVLRGRLDALAGALPGEPGQPWLGAGAELGETALAVLYG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCEEEEEEC
GSEVPASVAVLPLHQEHGVNGYLCLGSRDPERFSVGKATDLLERFAAFVSAGVVNVAHRE
CCCCCCCEEEEEEHHHCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QVARYGMTDMLTNLPNRRYFDSRIHDEMQRSARAKSPVACLFIDVDHFKRVNDTYGHAVG
HHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHH
DRALAAIGACIRKTVRHGDTVARYGGEEFVVLVRGDAVHACSVAERVRAAVAALVAAEVR
HHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
GDPVGLTVSIGVAVGNVGEIADGEAGAATRLVEHADQALYVAKHGGRNCVVLHRAAPEPA
CCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCC
SPCAPQAPALPAP
CCCCCCCCCCCCC
>Mature Secondary Structure
MERHMHALLDTVRHNERVLQRFQQIELRLVSATDFAVFHDTLVEALAQAFELPFVTLWLN
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEC
EDILLVREMLDAVSRAKPAAQAVLRGRLDALAGALPGEPGQPWLGAGAELGETALAVLYG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCEEEEEEC
GSEVPASVAVLPLHQEHGVNGYLCLGSRDPERFSVGKATDLLERFAAFVSAGVVNVAHRE
CCCCCCCEEEEEEHHHCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QVARYGMTDMLTNLPNRRYFDSRIHDEMQRSARAKSPVACLFIDVDHFKRVNDTYGHAVG
HHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHH
DRALAAIGACIRKTVRHGDTVARYGGEEFVVLVRGDAVHACSVAERVRAAVAALVAAEVR
HHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
GDPVGLTVSIGVAVGNVGEIADGEAGAATRLVEHADQALYVAKHGGRNCVVLHRAAPEPA
CCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEECCCCCCC
SPCAPQAPALPAP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]