Click here to switch to the map view.

The map label for this gene is mycA [H]

Identifier: 238027305

GI number: 238027305

Start: 1876290

End: 1878602

Strand: Direct

Name: mycA [H]

Synonym: bglu_1g16930

Alternate gene names: 238027305

Gene position: 1876290-1878602 (Clockwise)

Preceding gene: 339905965

Following gene: 238027306

Centisome position: 48.03

GC content: 73.02

Gene sequence:

>2313_bases
ATGACGCACTCGCATGATGCACGCCAGGCGGCCGGCGCGGCCGCCCTCGACATCGCGGTGATCGGCGTGGGCGCGCGCGT
GCCGGGCGCGCGCGGCCCCGACGCACTGTGGTCCCTGCTGATGTCGGGCGAGGACGGCCTGACCACCTTCACGCCCGAGC
AGCTGGCCGCCGAGCTGGAGGCGCTGGGCGAGGTCGATCGGGCTCGCTACGTGCCGCGCCGCGGCGTGATCGACGGCATC
GAGGCATTCGACGCGCCGTTCTTCAAGCTGTCGGCGGCCGAGGCGGACCTGCTCGATCCTCAGCACCGGGTACTCATGGA
GGTCGTCTGGGAGGCGCTGGAGGATGCTCAGGCCAGCGACTGCGCGGGTGCCTCGCTCGGCCTGTTCACCACCTCGGGCC
TGAGCCAGTACCTGATCCGCCACCTGCTGCCCGACCCGGCGCTGCGCGAGCGTCACGGCGAGTTGCAGCTGCTGATGCTC
AACGACAAGGACTTTCTTGCCACCCGCCTGGCCTATTTCCTCGATGTGCACGGGCCGGCCGTCAACCTGCAGACCGGCTG
TTCGAGCGGGCTGGTGGCGCTGCACTACGCCTGCCTGTCGCTGCAGCGCGGCGACTGCGCGGCAGCGGTGGTGGGGGGCG
TGTCGATCGCCTTGCCCCAGCAGTCGGGCTACGTCTACTCGGAGAACATGATCGGTTCGCGCGATGGCGTGTGCCGCGCG
TTCGATCGCGACGCCTCCGGCACGGTGCGCGGCAACGGCGCCTGCGCGGTGGTGCTCAAGCCGCTGGCGGCCGCGCTCGC
CGACGGCGATCCGGTGCGCGCCGTGATCAAGGGCAGCGCGCTCAACAACGACGGCCGCGACAAGGTCGGCTTCACGGCGC
CGAGCCCGCGCTGGCAGGCCGAGGTGATCGCGCGGGCCTATCGCGACTGCGGCGTCGCGCTCGACGACATCGACCTGGTC
GAGGCGCATGGCACCGGCACGCCGCTCGGCGACCCGATCGAGGCGCGCGCGCTCAACCAGGTGTTCGGCGGCGCAGGCAC
GGGCCGCACCCGCTATCTCGGCGCGCTGAAGACGCAGATCGGCCATCTCGACACCGCCGCCGGGCTCGCGGGGCTCGTCA
AGCTGTGCCTCGCGCTCGATCACGACACGATTCCGCCCACCGCGCATTTCCGCGAACTCAACCCGCGCATCGGCTTCGGT
GACGCGCCGTTTACCATCAACCGGGAGCCCGTCGCCTGGCCGCGCCGCGAGCGGCCCCGCTGTGCGGCGCTCAGCTCGTT
CGGCATCGGCGGCACCAATGCGCACGTGGTGGTGGCCGAGGCGCCGAACTGCGAGCCGCAAGCCTGCGACGGCCCGCAGG
CGATTGTCGTGTCGGCCCGCAGCGCCGCCTCGCTCGCGGCATTGCGAGATGCCTACGTGCAGGCGCTGGAAGCCATGAAC
GAGGCGGACGCAGCCGGCTTCGCCGCGTTCGCGGCGGCCACGCGCGTCGGCCGCCGTGCCTTCGAACATCGCCTGGCCAT
CAGCGCGAGCGGCCCCGCCGAGGCCGTCGCGCGGCTGCGGGCGGCGCCGGCGCGGGCGGCCGCGCCGCTTGCGGCCGGCG
TGCGGATCGCCGCCGAGCAGATGGCGGCGGTCGCGCAGCGGCTCGGCGTGACGCAGCATGCCGACCGGCCCGATGCGCTG
GCGGCCCGGCTCGCGGCGCTCGGCATCCGGCACGACCAGAGCGCGCCGCTGCGTCTCGACGTGTCGCAAGGCGCGTGGCG
CCTGGAGTGGGCCGACGGCGCGAGCTTGACGAGCGACGAGCCGCTGTCGACCCCGGCCGGATTGGACTTACAAATATTAC
TGTGGCGTGCCGGCATTGCGCTAGACTTTCGCCTCTCGGCAGGCAGGCGGGGCCGTCCGCCGGTCGCCGTGCCGACCTAT
CGATTCGAGCGGCAGCGCCACTGGATCGAGGCGGCTCGCGCGGGCGAGGCCGGCGCCGCCGCGCCGCCCGCGGCCGGCGC
GCCGCCTGCCACCCTGGTCGCGCTGGAGGCGCTGCTACTGGCGCAGTGGCGCGGGCTGCTCGGGCAACCGGCGTTGCGCG
GCATCGACAACGTGTTCGAACAGGGCGCGAACTCGCTGACCGTCGCGCAGTGCGTGGCGCAGCTCACCTCGACGCATGCG
CTGCCGATGCAAGTGGTGGACTGCTACGACGCACCGAGCGTTGCCGGGCAGGCACGCATGCTGGGCGAGCGCCTGGGGCT
CGCAGCAGGCCTCGCGCAGGCGCCCGCGGCTCCGCTGCCGGCGGCCGAGGTGGAGCAGTTCAACAACTTATAA

Upstream 100 bases:

>100_bases
CTCGCCGAGCGCGGCATCGACAAGGAACTCGACATCGTCGCGCTGTTCGAGCATGTCACCGTCGGCACGCTGGCCGACTT
CCTGGAGGAATGGACCGCCC

Downstream 100 bases:

>100_bases
GATCAACGAGAGAGAGCCGTGACAACCCGATACTTCCTGAAGAAGCATGTCAAGATCGAGCCGCTGGTCAATCGCTGGCA
CGCGAACCCCTGGCTCGTCT

Product: Thiotemplate mechanism natural product synthetase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MTHSHDARQAAGAAALDIAVIGVGARVPGARGPDALWSLLMSGEDGLTTFTPEQLAAELEALGEVDRARYVPRRGVIDGI
EAFDAPFFKLSAAEADLLDPQHRVLMEVVWEALEDAQASDCAGASLGLFTTSGLSQYLIRHLLPDPALRERHGELQLLML
NDKDFLATRLAYFLDVHGPAVNLQTGCSSGLVALHYACLSLQRGDCAAAVVGGVSIALPQQSGYVYSENMIGSRDGVCRA
FDRDASGTVRGNGACAVVLKPLAAALADGDPVRAVIKGSALNNDGRDKVGFTAPSPRWQAEVIARAYRDCGVALDDIDLV
EAHGTGTPLGDPIEARALNQVFGGAGTGRTRYLGALKTQIGHLDTAAGLAGLVKLCLALDHDTIPPTAHFRELNPRIGFG
DAPFTINREPVAWPRRERPRCAALSSFGIGGTNAHVVVAEAPNCEPQACDGPQAIVVSARSAASLAALRDAYVQALEAMN
EADAAGFAAFAAATRVGRRAFEHRLAISASGPAEAVARLRAAPARAAAPLAAGVRIAAEQMAAVAQRLGVTQHADRPDAL
AARLAALGIRHDQSAPLRLDVSQGAWRLEWADGASLTSDEPLSTPAGLDLQILLWRAGIALDFRLSAGRRGRPPVAVPTY
RFERQRHWIEAARAGEAGAAAPPAAGAPPATLVALEALLLAQWRGLLGQPALRGIDNVFEQGANSLTVAQCVAQLTSTHA
LPMQVVDCYDAPSVAGQARMLGERLGLAAGLAQAPAAPLPAAEVEQFNNL

Sequences:

>Translated_770_residues
MTHSHDARQAAGAAALDIAVIGVGARVPGARGPDALWSLLMSGEDGLTTFTPEQLAAELEALGEVDRARYVPRRGVIDGI
EAFDAPFFKLSAAEADLLDPQHRVLMEVVWEALEDAQASDCAGASLGLFTTSGLSQYLIRHLLPDPALRERHGELQLLML
NDKDFLATRLAYFLDVHGPAVNLQTGCSSGLVALHYACLSLQRGDCAAAVVGGVSIALPQQSGYVYSENMIGSRDGVCRA
FDRDASGTVRGNGACAVVLKPLAAALADGDPVRAVIKGSALNNDGRDKVGFTAPSPRWQAEVIARAYRDCGVALDDIDLV
EAHGTGTPLGDPIEARALNQVFGGAGTGRTRYLGALKTQIGHLDTAAGLAGLVKLCLALDHDTIPPTAHFRELNPRIGFG
DAPFTINREPVAWPRRERPRCAALSSFGIGGTNAHVVVAEAPNCEPQACDGPQAIVVSARSAASLAALRDAYVQALEAMN
EADAAGFAAFAAATRVGRRAFEHRLAISASGPAEAVARLRAAPARAAAPLAAGVRIAAEQMAAVAQRLGVTQHADRPDAL
AARLAALGIRHDQSAPLRLDVSQGAWRLEWADGASLTSDEPLSTPAGLDLQILLWRAGIALDFRLSAGRRGRPPVAVPTY
RFERQRHWIEAARAGEAGAAAPPAAGAPPATLVALEALLLAQWRGLLGQPALRGIDNVFEQGANSLTVAQCVAQLTSTHA
LPMQVVDCYDAPSVAGQARMLGERLGLAAGLAQAPAAPLPAAEVEQFNNL
>Mature_769_residues
THSHDARQAAGAAALDIAVIGVGARVPGARGPDALWSLLMSGEDGLTTFTPEQLAAELEALGEVDRARYVPRRGVIDGIE
AFDAPFFKLSAAEADLLDPQHRVLMEVVWEALEDAQASDCAGASLGLFTTSGLSQYLIRHLLPDPALRERHGELQLLMLN
DKDFLATRLAYFLDVHGPAVNLQTGCSSGLVALHYACLSLQRGDCAAAVVGGVSIALPQQSGYVYSENMIGSRDGVCRAF
DRDASGTVRGNGACAVVLKPLAAALADGDPVRAVIKGSALNNDGRDKVGFTAPSPRWQAEVIARAYRDCGVALDDIDLVE
AHGTGTPLGDPIEARALNQVFGGAGTGRTRYLGALKTQIGHLDTAAGLAGLVKLCLALDHDTIPPTAHFRELNPRIGFGD
APFTINREPVAWPRRERPRCAALSSFGIGGTNAHVVVAEAPNCEPQACDGPQAIVVSARSAASLAALRDAYVQALEAMNE
ADAAGFAAFAAATRVGRRAFEHRLAISASGPAEAVARLRAAPARAAAPLAAGVRIAAEQMAAVAQRLGVTQHADRPDALA
ARLAALGIRHDQSAPLRLDVSQGAWRLEWADGASLTSDEPLSTPAGLDLQILLWRAGIALDFRLSAGRRGRPPVAVPTYR
FERQRHWIEAARAGEAGAAAPPAAGAPPATLVALEALLLAQWRGLLGQPALRGIDNVFEQGANSLTVAQCVAQLTSTHAL
PMQVVDCYDAPSVAGQARMLGERLGLAAGLAQAPAAPLPAAEVEQFNNL

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=436, Percent_Identity=30.7339449541284, Blast_Score=189, Evalue=8e-48,
Organism=Homo sapiens, GI8923559, Length=442, Percent_Identity=23.7556561085973, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1787337, Length=285, Percent_Identity=30.1754385964912, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788663, Length=201, Percent_Identity=31.8407960199005, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI212642053, Length=493, Percent_Identity=29.4117647058824, Blast_Score=208, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI17550940, Length=364, Percent_Identity=36.5384615384615, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71983541, Length=278, Percent_Identity=26.978417266187, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71983535, Length=272, Percent_Identity=27.2058823529412, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24581345, Length=444, Percent_Identity=31.0810810810811, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI19920632, Length=418, Percent_Identity=30.3827751196172, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI221330659, Length=418, Percent_Identity=30.3827751196172, Blast_Score=164, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24644341, Length=461, Percent_Identity=26.4642082429501, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 80499; Mature: 80368

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50075 ACP_DOMAIN ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSHDARQAAGAAALDIAVIGVGARVPGARGPDALWSLLMSGEDGLTTFTPEQLAAELE
CCCCCCHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ALGEVDRARYVPRRGVIDGIEAFDAPFFKLSAAEADLLDPQHRVLMEVVWEALEDAQASD
HHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHCCCCCC
CAGASLGLFTTSGLSQYLIRHLLPDPALRERHGELQLLMLNDKDFLATRLAYFLDVHGPA
CCCCCCCCEEHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCE
VNLQTGCSSGLVALHYACLSLQRGDCAAAVVGGVSIALPQQSGYVYSENMIGSRDGVCRA
EEECCCCCCCHHHHHHHHHHCCCCCHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCHHH
FDRDASGTVRGNGACAVVLKPLAAALADGDPVRAVIKGSALNNDGRDKVGFTAPSPRWQA
CCCCCCCEEECCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
EVIARAYRDCGVALDDIDLVEAHGTGTPLGDPIEARALNQVFGGAGTGRTRYLGALKTQI
HHHHHHHHHCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GHLDTAAGLAGLVKLCLALDHDTIPPTAHFRELNPRIGFGDAPFTINREPVAWPRRERPR
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCC
CAALSSFGIGGTNAHVVVAEAPNCEPQACDGPQAIVVSARSAASLAALRDAYVQALEAMN
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
EADAAGFAAFAAATRVGRRAFEHRLAISASGPAEAVARLRAAPARAAAPLAAGVRIAAEQ
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAAVAQRLGVTQHADRPDALAARLAALGIRHDQSAPLRLDVSQGAWRLEWADGASLTSDE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCCC
PLSTPAGLDLQILLWRAGIALDFRLSAGRRGRPPVAVPTYRFERQRHWIEAARAGEAGAA
CCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCC
APPAAGAPPATLVALEALLLAQWRGLLGQPALRGIDNVFEQGANSLTVAQCVAQLTSTHA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
LPMQVVDCYDAPSVAGQARMLGERLGLAAGLAQAPAAPLPAAEVEQFNNL
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
THSHDARQAAGAAALDIAVIGVGARVPGARGPDALWSLLMSGEDGLTTFTPEQLAAELE
CCCCCHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
ALGEVDRARYVPRRGVIDGIEAFDAPFFKLSAAEADLLDPQHRVLMEVVWEALEDAQASD
HHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHCCCCCC
CAGASLGLFTTSGLSQYLIRHLLPDPALRERHGELQLLMLNDKDFLATRLAYFLDVHGPA
CCCCCCCCEEHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCE
VNLQTGCSSGLVALHYACLSLQRGDCAAAVVGGVSIALPQQSGYVYSENMIGSRDGVCRA
EEECCCCCCCHHHHHHHHHHCCCCCHHHHHHCCEEEEEECCCCEEEECCCCCCCCCCHHH
FDRDASGTVRGNGACAVVLKPLAAALADGDPVRAVIKGSALNNDGRDKVGFTAPSPRWQA
CCCCCCCEEECCCEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
EVIARAYRDCGVALDDIDLVEAHGTGTPLGDPIEARALNQVFGGAGTGRTRYLGALKTQI
HHHHHHHHHCCCEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GHLDTAAGLAGLVKLCLALDHDTIPPTAHFRELNPRIGFGDAPFTINREPVAWPRRERPR
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCC
CAALSSFGIGGTNAHVVVAEAPNCEPQACDGPQAIVVSARSAASLAALRDAYVQALEAMN
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
EADAAGFAAFAAATRVGRRAFEHRLAISASGPAEAVARLRAAPARAAAPLAAGVRIAAEQ
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAAVAQRLGVTQHADRPDALAARLAALGIRHDQSAPLRLDVSQGAWRLEWADGASLTSDE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCCC
PLSTPAGLDLQILLWRAGIALDFRLSAGRRGRPPVAVPTYRFERQRHWIEAARAGEAGAA
CCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCC
APPAAGAPPATLVALEALLLAQWRGLLGQPALRGIDNVFEQGANSLTVAQCVAQLTSTHA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
LPMQVVDCYDAPSVAGQARMLGERLGLAAGLAQAPAAPLPAAEVEQFNNL
CHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]