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The map label for this gene is kefA [H]

Identifier: 238027258

GI number: 238027258

Start: 1802125

End: 1804560

Strand: Reverse

Name: kefA [H]

Synonym: bglu_1g16450

Alternate gene names: 238027258

Gene position: 1804560-1802125 (Counterclockwise)

Preceding gene: 238027262

Following gene: 238027257

Centisome position: 46.19

GC content: 70.89

Gene sequence:

>2436_bases
ATGACGTGGATGCTGCTCGGCCTCATCGCCATGCCGCTCCGGCCCGCGCAGGCCGCGCCGTTCGCCGCCTCGGCCACCAG
CGCGAGCGCCGCCGCCGCGCCGTCGATCTCCTACGACGATGCCGTGGCCTCGCTCAAGCGCCTGCAGGCCGAGCAGGACC
GCATCAAGCAGCAGACCTCGGCCGCCACCAGCAACCAGCAGCTCGACACGCTCGACGCGGCCGCCGAGCAGCTCGGCACC
AACGTCGACAACCTGCTCGACGCGCTCACGCCGCAACGCGCGCAGCTGCAGGCGCAGCTCGACGTGCTCGGTCCTCCGCC
CGCGGCCGGCGCGGCGCCGGAAACGCCGGCCGTGGCGCGGCAGCGCGCCGACCTGACCGCGCGCCGCGACCAGCTCGATG
CGGTGATCAAGCAGGCCAGCGCCGAAAAGGTGGACCTCACCAACCTCAACCAGCAATTCGCGAAGCTGCGGCGCGGACTG
TTGCGCGACCAGCTCGCGCTGCGCTCGGGCAGCCTGCTCGGGCCGACGTTCTGGGCGCCGCTGCACACCCCGCTGCCCGG
CGACCGCCAGCGCCTGCACGCGTTCTTCGGCCAGATCGCCGCGCAGTTCGCGTTCGCCTGGGACCCCGAGCGGCGCGTCG
GCACGCTGGTGATGCTGGTGCTGGGCGTGGCGACCTGGAGCTTGGGCCGGCGCCTGCTCGAACGCGGCTTCTCGTGGTTC
TGCCTGAACCGGCTGCCGCCCACCCGGCTGCGCCGCAGCGCGCTGGCGCTGACCACCGCGCTCGCCACCCTGCTGGCCAC
CGGCACCGCCATGCAGCTGCTGCTGCTCGCGCTCACGCGCGGCGACGCGCTCACGCCCGCGCTCGACGACTTCGCCGGGA
AGCTCGCCGAGCTGGCCCTGACCTGCGCGCTGATCGCCGGCCTCGGCCGCGCGCTGCTCTGCACGCGCCATCCGAGCTGG
CGGCTGCCCGCCATCGCCGACCCGGTGGCGCTCGCGATGCAGCGCTTCCCGACCGTCCTGGCCGCGCTGCTGATGGTGTC
CGGCACGCTCGAACAGCTGAACCGGATCGCCGACACGAGCCTGCCCGTGACCGTGTTCGGGCGCGGTATCGTCGCGCTGG
TGGTGGCGCTGACGATCGGCGCCTCGCTGCTGCGCGCCAACCGCGCGCGCGCCGAGCTGGCCGCGGCCGGCGAGCCGCCC
GAGGCGCGCTCCACGCTGACCGGCCTGATCCACGCGGGCGTGACGCTGGCCGTGATCGCCTCGCTCGCCGCGCTCCTGAT
CGGCTACATCACGGTGGCGCGCTTCATCACCTACGAGCTGGTCTGGTTCGAGATCGTGCTGTGCAGCTTCTACGTGCTGA
CCCAGCTCACGCGCGACGTCTGCGCGAGCCTGTTCTCCACCAGCTACGCGAGCGGCAACACCATCAAGCATCTGTTCGGG
CTCGCCGATCACCATCTCGAACAGGCCTCGACGCTGCTGTCGGGCTGCGGCGTCGCGCTCCTGGTGCTGCTCGCCGCGCT
GGCGCTCGTCACGGGCGGCTTCGGCACCACCCCGAGCGACCTGCTCGACAGCATGCTCACGATGCTCGGCGGCACCCGGC
TGCGCAGTCTCAACATCGTGCCGGACCGCATCGTCAACGCGGTGCTCGCGCTGGTGATCGGCTTCTGGCTGCTGCGCTCG
ATCCGCCTCTGGCTCGACAACGAGTTCCTGCCCACGCTCGGCATGGACCCGGGCATGCGCGCCTCGCTCATCACGCTGTT
CAGCAACCTCGGCTACGTGCTGCTGGTGCTGATGACGCTGTCGCTGCTGGGCGTGCAGTGGAGCAGCCTGGCGTGGATCG
TCAGCGCGCTGTCGGTGGGGATCGGCTTCGGCCTGCAGGAGATCGTCAAGAACTTCGTGTCGGGGCTGATCCTGCTGACC
GAGCGGCCGGTGAAGGTGGGCGACATGGTCAGCATCAGCGGCGTGGAAGGCGACATCCGCCGCATCAACGTGCGCGCCAC
CGAAATCCAGCTCGCCGACCGCTCCACCGTGATCGTGCCGAACTCGCAGCTGATCTCGCAGAACCTGCGCAACGTGACGA
TGGGCAACAGCACCCAGGGGGTGGCCACCCTGATGCTGACGTTCCCGCTCAACACCGATCCCGAGCAGGTGCGCGACCTG
CTGCTCAACGCCTACCGGTCCCACCCCGGGATTCTGGAAAAGCCGGCGCCGTCGGTGACCTTCAGCCAGCTCACGCCGGA
CGGCATCACGCTCAGCGTGACCGGCTACGTGAACAGCCCGCGCGTGACGGCCTCGACCAAGAGCGACCTGCTGTTCGAGA
TCCTGAAACAGCTGCGCGCCGCCTCCATCACGCTGTCGAGCCCGCAGATGCTGATGATCCAGAACGCGCCGGGCAGCGAG
CCCGCCGAGGACACGGCCGCGAGCCGCCAGCCATGA

Upstream 100 bases:

>100_bases
GCTGGCGGCCCGCATCGCCGGCCGCCGCTGGCCGCGCCGACAAGAAAAAGGTGTTTCGTCCATGCCCCTGACCGCCCGAC
TGGCGCGCCTGCTCGCGCCA

Downstream 100 bases:

>100_bases
CCCCGGCTGCGCCGGCGGCTCGCGGGCCCCAGCGGGTCTTCAGCGGGCCTTCACCCGGCCGTCACGGCCGTCATCAACGG
TTGCAATCCCTTACCGCCGG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MTWMLLGLIAMPLRPAQAAPFAASATSASAAAAPSISYDDAVASLKRLQAEQDRIKQQTSAATSNQQLDTLDAAAEQLGT
NVDNLLDALTPQRAQLQAQLDVLGPPPAAGAAPETPAVARQRADLTARRDQLDAVIKQASAEKVDLTNLNQQFAKLRRGL
LRDQLALRSGSLLGPTFWAPLHTPLPGDRQRLHAFFGQIAAQFAFAWDPERRVGTLVMLVLGVATWSLGRRLLERGFSWF
CLNRLPPTRLRRSALALTTALATLLATGTAMQLLLLALTRGDALTPALDDFAGKLAELALTCALIAGLGRALLCTRHPSW
RLPAIADPVALAMQRFPTVLAALLMVSGTLEQLNRIADTSLPVTVFGRGIVALVVALTIGASLLRANRARAELAAAGEPP
EARSTLTGLIHAGVTLAVIASLAALLIGYITVARFITYELVWFEIVLCSFYVLTQLTRDVCASLFSTSYASGNTIKHLFG
LADHHLEQASTLLSGCGVALLVLLAALALVTGGFGTTPSDLLDSMLTMLGGTRLRSLNIVPDRIVNAVLALVIGFWLLRS
IRLWLDNEFLPTLGMDPGMRASLITLFSNLGYVLLVLMTLSLLGVQWSSLAWIVSALSVGIGFGLQEIVKNFVSGLILLT
ERPVKVGDMVSISGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNLRNVTMGNSTQGVATLMLTFPLNTDPEQVRDL
LLNAYRSHPGILEKPAPSVTFSQLTPDGITLSVTGYVNSPRVTASTKSDLLFEILKQLRAASITLSSPQMLMIQNAPGSE
PAEDTAASRQP

Sequences:

>Translated_811_residues
MTWMLLGLIAMPLRPAQAAPFAASATSASAAAAPSISYDDAVASLKRLQAEQDRIKQQTSAATSNQQLDTLDAAAEQLGT
NVDNLLDALTPQRAQLQAQLDVLGPPPAAGAAPETPAVARQRADLTARRDQLDAVIKQASAEKVDLTNLNQQFAKLRRGL
LRDQLALRSGSLLGPTFWAPLHTPLPGDRQRLHAFFGQIAAQFAFAWDPERRVGTLVMLVLGVATWSLGRRLLERGFSWF
CLNRLPPTRLRRSALALTTALATLLATGTAMQLLLLALTRGDALTPALDDFAGKLAELALTCALIAGLGRALLCTRHPSW
RLPAIADPVALAMQRFPTVLAALLMVSGTLEQLNRIADTSLPVTVFGRGIVALVVALTIGASLLRANRARAELAAAGEPP
EARSTLTGLIHAGVTLAVIASLAALLIGYITVARFITYELVWFEIVLCSFYVLTQLTRDVCASLFSTSYASGNTIKHLFG
LADHHLEQASTLLSGCGVALLVLLAALALVTGGFGTTPSDLLDSMLTMLGGTRLRSLNIVPDRIVNAVLALVIGFWLLRS
IRLWLDNEFLPTLGMDPGMRASLITLFSNLGYVLLVLMTLSLLGVQWSSLAWIVSALSVGIGFGLQEIVKNFVSGLILLT
ERPVKVGDMVSISGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNLRNVTMGNSTQGVATLMLTFPLNTDPEQVRDL
LLNAYRSHPGILEKPAPSVTFSQLTPDGITLSVTGYVNSPRVTASTKSDLLFEILKQLRAASITLSSPQMLMIQNAPGSE
PAEDTAASRQP
>Mature_810_residues
TWMLLGLIAMPLRPAQAAPFAASATSASAAAAPSISYDDAVASLKRLQAEQDRIKQQTSAATSNQQLDTLDAAAEQLGTN
VDNLLDALTPQRAQLQAQLDVLGPPPAAGAAPETPAVARQRADLTARRDQLDAVIKQASAEKVDLTNLNQQFAKLRRGLL
RDQLALRSGSLLGPTFWAPLHTPLPGDRQRLHAFFGQIAAQFAFAWDPERRVGTLVMLVLGVATWSLGRRLLERGFSWFC
LNRLPPTRLRRSALALTTALATLLATGTAMQLLLLALTRGDALTPALDDFAGKLAELALTCALIAGLGRALLCTRHPSWR
LPAIADPVALAMQRFPTVLAALLMVSGTLEQLNRIADTSLPVTVFGRGIVALVVALTIGASLLRANRARAELAAAGEPPE
ARSTLTGLIHAGVTLAVIASLAALLIGYITVARFITYELVWFEIVLCSFYVLTQLTRDVCASLFSTSYASGNTIKHLFGL
ADHHLEQASTLLSGCGVALLVLLAALALVTGGFGTTPSDLLDSMLTMLGGTRLRSLNIVPDRIVNAVLALVIGFWLLRSI
RLWLDNEFLPTLGMDPGMRASLITLFSNLGYVLLVLMTLSLLGVQWSSLAWIVSALSVGIGFGLQEIVKNFVSGLILLTE
RPVKVGDMVSISGVEGDIRRINVRATEIQLADRSTVIVPNSQLISQNLRNVTMGNSTQGVATLMLTFPLNTDPEQVRDLL
LNAYRSHPGILEKPAPSVTFSQLTPDGITLSVTGYVNSPRVTASTKSDLLFEILKQLRAASITLSSPQMLMIQNAPGSEP
AEDTAASRQP

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=270, Percent_Identity=32.962962962963, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI2367355, Length=243, Percent_Identity=34.156378600823, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1789291, Length=208, Percent_Identity=25.9615384615385, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 86722; Mature: 86591

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HEEEECCCCEEEEECCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
TWMLLGLIAMPLRPAQAAPFAASATSASAAAAPSISYDDAVASLKRLQAEQDRIKQQTS
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AATSNQQLDTLDAAAEQLGTNVDNLLDALTPQRAQLQAQLDVLGPPPAAGAAPETPAVAR
HHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
QRADLTARRDQLDAVIKQASAEKVDLTNLNQQFAKLRRGLLRDQLALRSGSLLGPTFWAP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LHTPLPGDRQRLHAFFGQIAAQFAFAWDPERRVGTLVMLVLGVATWSLGRRLLERGFSWF
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
CLNRLPPTRLRRSALALTTALATLLATGTAMQLLLLALTRGDALTPALDDFAGKLAELAL
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TCALIAGLGRALLCTRHPSWRLPAIADPVALAMQRFPTVLAALLMVSGTLEQLNRIADTS
HHHHHHHCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPVTVFGRGIVALVVALTIGASLLRANRARAELAAAGEPPEARSTLTGLIHAGVTLAVIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
SLAALLIGYITVARFITYELVWFEIVLCSFYVLTQLTRDVCASLFSTSYASGNTIKHLFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LADHHLEQASTLLSGCGVALLVLLAALALVTGGFGTTPSDLLDSMLTMLGGTRLRSLNIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCC
PDRIVNAVLALVIGFWLLRSIRLWLDNEFLPTLGMDPGMRASLITLFSNLGYVLLVLMTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLLGVQWSSLAWIVSALSVGIGFGLQEIVKNFVSGLILLTERPVKVGDMVSISGVEGDIR
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCCCCE
RINVRATEIQLADRSTVIVPNSQLISQNLRNVTMGNSTQGVATLMLTFPLNTDPEQVRDL
EEEEEEEEEEEECCCEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHH
LLNAYRSHPGILEKPAPSVTFSQLTPDGITLSVTGYVNSPRVTASTKSDLLFEILKQLRA
HHHHHHCCCCCCCCCCCCCCHHCCCCCCEEEEEEEECCCCCEECCCHHHHHHHHHHHHHH
ASITLSSPQMLMIQNAPGSEPAEDTAASRQP
HEEEECCCCEEEEECCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]