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The map label for this gene is smaIM [H]

Identifier: 238027159

GI number: 238027159

Start: 1701361

End: 1702428

Strand: Direct

Name: smaIM [H]

Synonym: bglu_1g15450

Alternate gene names: 238027159

Gene position: 1701361-1702428 (Clockwise)

Preceding gene: 238027157

Following gene: 238027160

Centisome position: 43.55

GC content: 65.36

Gene sequence:

>1068_bases
ATGACCTGGCTCGACCGTTCCCACCTTGGCGACTGCCGCGACCTGATGCGCACCATGATCGCCGACGGCGTGCGCGTGCA
GACGATCGTCACCAGCCCACCGTATTGGGGTCTGCGCTCGTATCTGCCCGATGGCCACCCCGACAAGGCGCGCGAGATCG
GCCAGGAGCCGACTCTGCGCGAGTTCATCGACACGCTCGTCGAGGTGTTCAGCCTCGCGCGCCAGCTGCTGGCCGACGAC
GGCACGGCCTGGATCAACATGGGCGACAGCTATGCAGCGAATCGCTCCTATCAAGTTGCGTCCACGCTGATGAATGGCGA
CGCGACAAATCAAGCTCAGGCGGCCGGTGGGCGCGGAATGAAAGTGCCTGATGGCCTGAAGCCAAAGGATCTAGTCGGCC
AGCCGTGGCGCCTCGCCTTCGCGCTTCAGGACGCCGGCTGGTATCTCCGGCAGGACATCATTTGGGCGAAGCCCAACCCG
ATGCCAGAGAGCGTGCGCGACCGTTGCACGAAGGCGCATGAGTACCTGTTCCTGCTCAGCAAGAGCGAGCGGTACTACTT
CGACCATCAGGCGATGCGCGAGCCGCGCACTCAGGACGAGGACAGCAGCGTTTTTCGGGGTGGATGCTACGTCGGTGGGG
CAGACGATAACGCAGTGCTTGGTAAGCGCCGCGTTCCCGGCAACAAGTCACACAAGGGCACGACCGCGTCGGCCAATGAC
GAGAAGCATCGTACAAAGGCTGGGCTCGTCGACTACGCGCAGCGGGAGCGCGACGCAGACCCCGGCGACCTTGGCACTCG
AAATAGGCGCAGCGTCTGGACCATCGCCACGCAGCCCTACGCGGCCGCCCATTTCGCCACTTTCCCCGAGGCGCTCGTCG
AGCCCTGCGTGCTCGCTGGCAGCCGTCCGGGCGACGTCGTCTTCGATCCGTTCTTCGGCAGTGGCACCGTCGGCCAGGTC
GCACAGCGGCTCGGCCGGCGCTTTCTCGGCTGCGAACTCAATCCCGACTACGAGCCGCTGCAGCGCGATCGCCTGCGGCA
GCCCGGCCTGATGTTGGAGGCCTCATGA

Upstream 100 bases:

>100_bases
ATGTCCATATCGCTCCCCGAGTTGATCATATTTGTACGCACGCGCCATTCGTTCATCTTAGCGCATATGCCTTGATCTAC
CGATCGGTTTGCGTGTCCAA

Downstream 100 bases:

>100_bases
GCCTCATCATCGCCCCGATCTCGTTCGACGAGGCCAATGCCTTCGTCGCGACGCATCACCGGCACCACGCACCTGCGGTC
GGCCACAAGTTCAGCATCGC

Product: DNA modification methylase

Products: NA

Alternate protein names: M.SmaI; N-4 cytosine-specific methyltransferase SmaI [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTWLDRSHLGDCRDLMRTMIADGVRVQTIVTSPPYWGLRSYLPDGHPDKAREIGQEPTLREFIDTLVEVFSLARQLLADD
GTAWINMGDSYAANRSYQVASTLMNGDATNQAQAAGGRGMKVPDGLKPKDLVGQPWRLAFALQDAGWYLRQDIIWAKPNP
MPESVRDRCTKAHEYLFLLSKSERYYFDHQAMREPRTQDEDSSVFRGGCYVGGADDNAVLGKRRVPGNKSHKGTTASAND
EKHRTKAGLVDYAQRERDADPGDLGTRNRRSVWTIATQPYAAAHFATFPEALVEPCVLAGSRPGDVVFDPFFGSGTVGQV
AQRLGRRFLGCELNPDYEPLQRDRLRQPGLMLEAS

Sequences:

>Translated_355_residues
MTWLDRSHLGDCRDLMRTMIADGVRVQTIVTSPPYWGLRSYLPDGHPDKAREIGQEPTLREFIDTLVEVFSLARQLLADD
GTAWINMGDSYAANRSYQVASTLMNGDATNQAQAAGGRGMKVPDGLKPKDLVGQPWRLAFALQDAGWYLRQDIIWAKPNP
MPESVRDRCTKAHEYLFLLSKSERYYFDHQAMREPRTQDEDSSVFRGGCYVGGADDNAVLGKRRVPGNKSHKGTTASAND
EKHRTKAGLVDYAQRERDADPGDLGTRNRRSVWTIATQPYAAAHFATFPEALVEPCVLAGSRPGDVVFDPFFGSGTVGQV
AQRLGRRFLGCELNPDYEPLQRDRLRQPGLMLEAS
>Mature_354_residues
TWLDRSHLGDCRDLMRTMIADGVRVQTIVTSPPYWGLRSYLPDGHPDKAREIGQEPTLREFIDTLVEVFSLARQLLADDG
TAWINMGDSYAANRSYQVASTLMNGDATNQAQAAGGRGMKVPDGLKPKDLVGQPWRLAFALQDAGWYLRQDIIWAKPNPM
PESVRDRCTKAHEYLFLLSKSERYYFDHQAMREPRTQDEDSSVFRGGCYVGGADDNAVLGKRRVPGNKSHKGTTASANDE
KHRTKAGLVDYAQRERDADPGDLGTRNRRSVWTIATQPYAAAHFATFPEALVEPCVLAGSRPGDVVFDPFFGSGTVGQVA
QRLGRRFLGCELNPDYEPLQRDRLRQPGLMLEAS

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 39595; Mature: 39464

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLDRSHLGDCRDLMRTMIADGVRVQTIVTSPPYWGLRSYLPDGHPDKAREIGQEPTLR
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCCCHH
EFIDTLVEVFSLARQLLADDGTAWINMGDSYAANRSYQVASTLMNGDATNQAQAAGGRGM
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCHHHHHHHHCCCCCCHHHHCCCCCC
KVPDGLKPKDLVGQPWRLAFALQDAGWYLRQDIIWAKPNPMPESVRDRCTKAHEYLFLLS
CCCCCCCCHHHCCCCEEEEEEEECCCCCHHHCEEEECCCCCCHHHHHHHHHHHHEEEEEE
KSERYYFDHQAMREPRTQDEDSSVFRGGCYVGGADDNAVLGKRRVPGNKSHKGTTASAND
CCCCEEECHHHHCCCCCCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EKHRTKAGLVDYAQRERDADPGDLGTRNRRSVWTIATQPYAAAHFATFPEALVEPCVLAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCC
SRPGDVVFDPFFGSGTVGQVAQRLGRRFLGCELNPDYEPLQRDRLRQPGLMLEAS
CCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEECC
>Mature Secondary Structure 
TWLDRSHLGDCRDLMRTMIADGVRVQTIVTSPPYWGLRSYLPDGHPDKAREIGQEPTLR
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCCHHHHHCCCCCCHH
EFIDTLVEVFSLARQLLADDGTAWINMGDSYAANRSYQVASTLMNGDATNQAQAAGGRGM
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCHHHHHHHHCCCCCCHHHHCCCCCC
KVPDGLKPKDLVGQPWRLAFALQDAGWYLRQDIIWAKPNPMPESVRDRCTKAHEYLFLLS
CCCCCCCCHHHCCCCEEEEEEEECCCCCHHHCEEEECCCCCCHHHHHHHHHHHHEEEEEE
KSERYYFDHQAMREPRTQDEDSSVFRGGCYVGGADDNAVLGKRRVPGNKSHKGTTASAND
CCCCEEECHHHHCCCCCCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EKHRTKAGLVDYAQRERDADPGDLGTRNRRSVWTIATQPYAAAHFATFPEALVEPCVLAG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCC
SRPGDVVFDPFFGSGTVGQVAQRLGRRFLGCELNPDYEPLQRDRLRQPGLMLEAS
CCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690008; 2251121 [H]