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The map label for this gene is proP [H]

Identifier: 238026769

GI number: 238026769

Start: 1235638

End: 1237119

Strand: Reverse

Name: proP [H]

Synonym: bglu_1g11270

Alternate gene names: 238026769

Gene position: 1237119-1235638 (Counterclockwise)

Preceding gene: 238026771

Following gene: 238026757

Centisome position: 31.67

GC content: 68.96

Gene sequence:

>1482_bases
TTGACTGCCTCACACGTCAGCCCCTCCGCTGCTTGCGCGTCGGCCACCGACGCCCCCGTCACCGTCAGCGACATCACCGT
GGTCGACCAGAGCCTGCTCAAGCGCGCGGTGCGCGCGATGGCCATCGGCAACGCCATGGAATGGTTCGACTTCGGCGTGT
ACAGCTACATCGCCGTCACGCTCGGCCAGGTGTTCTTCCCCTCCAGCAACCCGTCCGCGCAGCTGCTCGCCACGTTCGGC
ACGTTCGCGGCCGCGTTCCTGGTGCGCCCGCTCGGCGGCATGGTGTTCGGCCCGCTCGGCGACCGCATCGGCCGTCAGCG
CGTGCTGGCCATGACGATGATCATGATGGCGCTCGGCACGTTCTCGATCGGCCTGATTCCGAGCTACCAATCGATCGGCA
TCCTCGCACCGGTGCTGCTGCTGGTCGCGCGCCTCGTGCAGGGCTTCTCGACGGGCGGCGAGTACGGCGGGGCCGCCACC
TTCATCGCCGAATTCTCCACCGACAAGCGCCGCGGCTTCATGGGCAGCTTTCTCGAATTCGGCACGCTGATCGGCTACGT
GCTCGGCGCGGGCACGGTCGCGGCGCTGGCGGCGCTGCTGCCGCATGACGCCCTGCTGTCGTGGGGCTGGCGCGTGCCGT
TCCTGGTGGCCGGCCCGCTCGGCCTGATCGGGCTCTACATCCGCATGAAGCTCGAGGAGACGCCGGCCTTCAAGCGCCAG
GCCGAACTGCGCGAGGCGCAGGACAAGTCGATGCCGAAGCTCAAGTTCACCGAGACCCTCGGCCGCCACTGGCGCGCGAT
GCTGCTCTGCGTGGGCCTCGTGCTGATCTTCAACGTGACCGACTACATGGCGCTGTCGTACCTGCCGAGCTATCTGTCGT
CGACGCTGCACTTCAACGAGACGCACGGCCTGCTGCTGGTGCTGCTCGTGATGGTGCTGATGATGCCGATGACGCTCGCC
GCCGGCCGCCTGTCCGACACGGTCGGCCGCAAGCCGGTGATGCTGGCCGGCTGTCTCGGCCTGATCGCGCTGTCGATCCC
GGCGCTGATGCTGATCCGCACGGGCGAAATGCTGCCGGTGTTCGGCGGGCTGATGATCCTCGGCGTGCTGCTCTCGTGCT
TCTGCGGCGTGATGCCGTCGGCGCTGCCGGCGCTGTTCCCGACCGAGATCCGCTACGGCGCGCTCGCGATCGGCTTCAAC
ATCTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTCGTCACGGCGTGGCTGGTCGATCGCACCGGCAACCTGATGATGCC
CGCCTACTACCTGATGGGGGCCGCCGTGATCGGCGCGGTGTCGGTGCTCACGCTGCGCGAGACGGCACGCCAGCCGCTGG
AAGGCTCGCCGCCCGCCGTCGCCTCGCGCCGCGAAGCGCATGCGCTGGTGCGCGAACTGCGCGAGCAGGCAACCAACGAC
GACAGCCCCAGCTACCCGGGCGTGTCGGCCGCGCGGGCATAA

Upstream 100 bases:

>100_bases
CCGACGTTCCGTGCCGCCACGCCGCTGCGCTCCCGGCAAACCGTGGCGAAACGCGTGCCGCCAGGCGCATGCTTCGTCCG
CTGTCAAAGGAGCCCAAATC

Downstream 100 bases:

>100_bases
GCCCGGCGGCTAGCCGCCGCCCGCCCCGCTCCACGACGCCGCGCCGACCGCTCCCGGTCGGCGCGGCGTTTTTTATCGGC
GGGCCGCGCTGCCGCCGGCC

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTASHVSPSAACASATDAPVTVSDITVVDQSLLKRAVRAMAIGNAMEWFDFGVYSYIAVTLGQVFFPSSNPSAQLLATFG
TFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMALGTFSIGLIPSYQSIGILAPVLLLVARLVQGFSTGGEYGGAAT
FIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGTVAALAALLPHDALLSWGWRVPFLVAGPLGLIGLYIRMKLEETPAFKRQ
AELREAQDKSMPKLKFTETLGRHWRAMLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFNETHGLLLVLLVMVLMMPMTLA
AGRLSDTVGRKPVMLAGCLGLIALSIPALMLIRTGEMLPVFGGLMILGVLLSCFCGVMPSALPALFPTEIRYGALAIGFN
ISVSLFGGTTPLVTAWLVDRTGNLMMPAYYLMGAAVIGAVSVLTLRETARQPLEGSPPAVASRREAHALVRELREQATND
DSPSYPGVSAARA

Sequences:

>Translated_493_residues
MTASHVSPSAACASATDAPVTVSDITVVDQSLLKRAVRAMAIGNAMEWFDFGVYSYIAVTLGQVFFPSSNPSAQLLATFG
TFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMALGTFSIGLIPSYQSIGILAPVLLLVARLVQGFSTGGEYGGAAT
FIAEFSTDKRRGFMGSFLEFGTLIGYVLGAGTVAALAALLPHDALLSWGWRVPFLVAGPLGLIGLYIRMKLEETPAFKRQ
AELREAQDKSMPKLKFTETLGRHWRAMLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFNETHGLLLVLLVMVLMMPMTLA
AGRLSDTVGRKPVMLAGCLGLIALSIPALMLIRTGEMLPVFGGLMILGVLLSCFCGVMPSALPALFPTEIRYGALAIGFN
ISVSLFGGTTPLVTAWLVDRTGNLMMPAYYLMGAAVIGAVSVLTLRETARQPLEGSPPAVASRREAHALVRELREQATND
DSPSYPGVSAARA
>Mature_492_residues
TASHVSPSAACASATDAPVTVSDITVVDQSLLKRAVRAMAIGNAMEWFDFGVYSYIAVTLGQVFFPSSNPSAQLLATFGT
FAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMALGTFSIGLIPSYQSIGILAPVLLLVARLVQGFSTGGEYGGAATF
IAEFSTDKRRGFMGSFLEFGTLIGYVLGAGTVAALAALLPHDALLSWGWRVPFLVAGPLGLIGLYIRMKLEETPAFKRQA
ELREAQDKSMPKLKFTETLGRHWRAMLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFNETHGLLLVLLVMVLMMPMTLAA
GRLSDTVGRKPVMLAGCLGLIALSIPALMLIRTGEMLPVFGGLMILGVLLSCFCGVMPSALPALFPTEIRYGALAIGFNI
SVSLFGGTTPLVTAWLVDRTGNLMMPAYYLMGAAVIGAVSVLTLRETARQPLEGSPPAVASRREAHALVRELREQATNDD
SPSYPGVSAARA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=481, Percent_Identity=49.6881496881497, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=31.6397228637413, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=34.4139650872818, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=33.5680751173709, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87082231, Length=435, Percent_Identity=22.5287356321839, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=23.1607629427793, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52561; Mature: 52430

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASHVSPSAACASATDAPVTVSDITVVDQSLLKRAVRAMAIGNAMEWFDFGVYSYIAVT
CCCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LGQVFFPSSNPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMALGT
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHH
FSIGLIPSYQSIGILAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHH
GTLIGYVLGAGTVAALAALLPHDALLSWGWRVPFLVAGPLGLIGLYIRMKLEETPAFKRQ
HHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHCHHHHHHHHHHEEECCCCHHHHH
AELREAQDKSMPKLKFTETLGRHWRAMLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFNE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
THGLLLVLLVMVLMMPMTLAAGRLSDTVGRKPVMLAGCLGLIALSIPALMLIRTGEMLPV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FGGLMILGVLLSCFCGVMPSALPALFPTEIRYGALAIGFNISVSLFGGTTPLVTAWLVDR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEEEEEEEEEECCCHHHHHHHHHHC
TGNLMMPAYYLMGAAVIGAVSVLTLRETARQPLEGSPPAVASRREAHALVRELREQATND
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DSPSYPGVSAARA
CCCCCCCCCCCCC
>Mature Secondary Structure 
TASHVSPSAACASATDAPVTVSDITVVDQSLLKRAVRAMAIGNAMEWFDFGVYSYIAVT
CCCCCCCHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH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CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]