Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 238026688

GI number: 238026688

Start: 1139208

End: 1141139

Strand: Reverse

Name: thiC [H]

Synonym: bglu_1g10430

Alternate gene names: 238026688

Gene position: 1141139-1139208 (Counterclockwise)

Preceding gene: 238026690

Following gene: 238026687

Centisome position: 29.21

GC content: 66.61

Gene sequence:

>1932_bases
ATGAATGCCAATCCGAAGTTCCTGTCGGCCAATGCCCGCGTCGACGCCGCCGCGATCGCCCCGCTGCCGAATTCACGCAA
GGTCTACGTCACCGGCTCGCAGCCCGACATCCGCGTGCCGATGCGCGAGATCACGCAGTCGGACACCCCGACCGGCTTCG
GCGGCGAGAAGAACCCGCCGATCTACGTCTATGACACGTCCGGCCCGTACACCGACCCGGAAGCCCGGATCGACATCCGC
TCGGGCCTGCCGGCGCTGCGCCAGCGCTGGATCGAGGCGCGCGGCGACACCGAGATGCTGGCGGGCCTGACGAGCGAGTA
CGGCCGCGAGCGCGCCGCCGACCCGGCCACCGCCGAACTGCGCTTCCCGGGCCTGCATCGCCAGCCGCGCCGTGCGCAGC
GCGGCCGCAACGTCACGCAGATGCACTACGCGCGCCAGGGCATCATCACGCCCGAGATGGAATTCATCGCGATCCGCGAG
AACCAGCGCCGCGCCGAGTACCTCGCGAGCCTCGAGGCAAGCGGCCCGAACGGCGCCAAGCTGGCCGCCATGATTGGCCG
CCAGCATCGCGGCCAGGCGTTCGGCGCAGCCGCGTTCGGCGCGCAGGCGCTCACCGAGATCACGCCCGAGTTCGTGCGCG
AGGAAGTGGCGCGCGGCCGCGCCATCATCCCGGCCAACATCAACCACCCCGAATCGGAGCCGATGATCATCGGCCGCAAC
TTCCTCGTGAAGATCAACGCGAACATCGGCAATTCGGCCGTGACCTCGTCGATCGGCGAGGAAGTCGACAAGATGACCTG
GGCGATCCGCTGGGGCGGCGACACGGTGATGGACCTGTCCACCGGCAAGCACATCCACGAGACGCGCGAGTGGATCATCC
GCAACAGCCCGGTGCCGATCGGCACCGTACCGATCTACCAGGCGCTGGAAAAGGTCAACGGCCGCGCCGAGGACCTCACC
TGGGAAATCTTCCGCGACACGCTGATCGAGCAGGCCGAGCAAGGCGTCGACTACTTCACGATCCATGCCGGCGTGCGCCT
GCAGTACGTGCCGCTCACGGCCAACCGGATGACCGGCATCGTCTCGCGCGGCGGCTCGATCATGGCGAAGTGGTGCCTCG
CGCATCACCAGGAAAGCTTCCTCTACGAGCACTTCGAGGACATCTGCGAGATCATGAAGGCCTACGACGTGAGCTTCTCG
CTCGGCGACGGCCTGCGCCCCGGCTCGATCTACGACGCCAACGACGAAGCCCAGCTCGGCGAGCTGAAGACGCTCGGCGA
ACTCACCCAGATCGCCTGGAAGCACGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGATGCAATTGATCAAGG
AGAACATGGACCTGCAGCTCGAATGGTGCGACGAGGCGCCGTTCTACACGCTCGGGCCGCTCACCACCGACATCGCGCCG
GGCTACGACCACATCACGTCGGGCATCGGCGCGGCGATGATCGGCTGGTTCGGCACCGCGATGCTCTGCTACGTCACGCC
GAAGGAGCACCTCGGGCTGCCGAACAAGGACGACGTCAAGGAAGGCATCATCACCTACAAGCTCGCGGCACACGCGGCCG
ACCTCGCCAAGGGCCATCCGGGCGCGCAGGTGCGCGACAACGCGCTGTCGAAGGCGCGCTTCGAGTTCCGCTGGGAAGAC
CAGTTCAACATCGGCCTCGACCCGGACAAGGCGCGTGAATTCCACGACGAGACGCTGCCGAAGGATTCGGCCAAGGTCGC
GCACTTCTGCTCGATGTGCGGCCCGCACTTCTGCTCGATGAAGATCACGCAGGACGTGCGCGATTTCGCGGCCAAACAGG
GCGTGTCGGAAAGCGAGGCGCTGAAGAAGGGCATGGAGGTCAAGGCCGTCGAGTTCGTCAGGACCGGCGCCGAAATCTAC
CACCGCCAGTGA

Upstream 100 bases:

>100_bases
CGCAGGGAAGCGTACAGGCCTCGACCCCTTGCCTCATCCTCCGGCCGGCCTGCCGCCCATCTCGTCTCCTGCTTAGCGCC
CCACCTCAGGAGATGACTGC

Downstream 100 bases:

>100_bases
GCCCGGGCGCGGCGGCCCGCGCCGCCGCGCCGCTCGCGCAGCAAGGTTCATACCCGCGCAGATACATTTGATTACGCGCG
CAAACTCGCTTAACAAGTGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MNANPKFLSANARVDAAAIAPLPNSRKVYVTGSQPDIRVPMREITQSDTPTGFGGEKNPPIYVYDTSGPYTDPEARIDIR
SGLPALRQRWIEARGDTEMLAGLTSEYGRERAADPATAELRFPGLHRQPRRAQRGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLASLEASGPNGAKLAAMIGRQHRGQAFGAAAFGAQALTEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGRAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHQESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDFAAKQGVSESEALKKGMEVKAVEFVRTGAEIY
HRQ

Sequences:

>Translated_643_residues
MNANPKFLSANARVDAAAIAPLPNSRKVYVTGSQPDIRVPMREITQSDTPTGFGGEKNPPIYVYDTSGPYTDPEARIDIR
SGLPALRQRWIEARGDTEMLAGLTSEYGRERAADPATAELRFPGLHRQPRRAQRGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLASLEASGPNGAKLAAMIGRQHRGQAFGAAAFGAQALTEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGRAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHQESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDFAAKQGVSESEALKKGMEVKAVEFVRTGAEIY
HRQ
>Mature_643_residues
MNANPKFLSANARVDAAAIAPLPNSRKVYVTGSQPDIRVPMREITQSDTPTGFGGEKNPPIYVYDTSGPYTDPEARIDIR
SGLPALRQRWIEARGDTEMLAGLTSEYGRERAADPATAELRFPGLHRQPRRAQRGRNVTQMHYARQGIITPEMEFIAIRE
NQRRAEYLASLEASGPNGAKLAAMIGRQHRGQAFGAAAFGAQALTEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGRAEDLT
WEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGIVSRGGSIMAKWCLAHHQESFLYEHFEDICEIMKAYDVSFS
LGDGLRPGSIYDANDEAQLGELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHPGAQVRDNALSKARFEFRWED
QFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSMKITQDVRDFAAKQGVSESEALKKGMEVKAVEFVRTGAEIY
HRQ

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=637, Percent_Identity=69.2307692307692, Blast_Score=902, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 71413; Mature: 71413

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANPKFLSANARVDAAAIAPLPNSRKVYVTGSQPDIRVPMREITQSDTPTGFGGEKNPP
CCCCCCEEECCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCC
IYVYDTSGPYTDPEARIDIRSGLPALRQRWIEARGDTEMLAGLTSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEE
RFPGLHRQPRRAQRGRNVTQMHYARQGIITPEMEFIAIRENQRRAEYLASLEASGPNGAK
ECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCHH
LAAMIGRQHRGQAFGAAAFGAQALTEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGRAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHQESFLYEHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
KITQDVRDFAAKQGVSESEALKKGMEVKAVEFVRTGAEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MNANPKFLSANARVDAAAIAPLPNSRKVYVTGSQPDIRVPMREITQSDTPTGFGGEKNPP
CCCCCCEEECCCCCCEEEECCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCC
IYVYDTSGPYTDPEARIDIRSGLPALRQRWIEARGDTEMLAGLTSEYGRERAADPATAEL
EEEEECCCCCCCCCCEEEHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEE
RFPGLHRQPRRAQRGRNVTQMHYARQGIITPEMEFIAIRENQRRAEYLASLEASGPNGAK
ECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCHH
LAAMIGRQHRGQAFGAAAFGAQALTEITPEFVREEVARGRAIIPANINHPESEPMIIGRN
HHHHHCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCE
FLVKINANIGNSAVTSSIGEEVDKMTWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVPI
EEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCC
GTVPIYQALEKVNGRAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLQYVPLTANRMTGI
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHH
VSRGGSIMAKWCLAHHQESFLYEHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQLG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCCCHHH
ELKTLGELTQIAWKHDVQVMIEGPGHVPMQLIKENMDLQLEWCDEAPFYTLGPLTTDIAP
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEECCCCCCCCC
GYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPNKDDVKEGIITYKLAAHAADLAKGHP
CHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCHHHHHHEEEHHHHHHHHHHCCCC
GAQVRDNALSKARFEFRWEDQFNIGLDPDKAREFHDETLPKDSAKVAHFCSMCGPHFCSM
CCCHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
KITQDVRDFAAKQGVSESEALKKGMEVKAVEFVRTGAEIYHRQ
HHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA