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The map label for this gene is pmbA [H]

Identifier: 238026571

GI number: 238026571

Start: 991191

End: 992561

Strand: Direct

Name: pmbA [H]

Synonym: bglu_1g09150

Alternate gene names: 238026571

Gene position: 991191-992561 (Clockwise)

Preceding gene: 238026568

Following gene: 238026573

Centisome position: 25.37

GC content: 69.58

Gene sequence:

>1371_bases
ATGCCTGCCCATATCGACGCACCCGCGCGCCATTTCCCGCACACGCAGGAGCAACTGAAAGAAATCGCCTCGGACATCCT
CCGGCACGCGAAGTCGCTCGGCGCGACCGACGCGGCGACCGAAATCTCCGAAGGCGACGGCCTGTCGGTGTCGGTGCGGC
GCGGCGAAGTCGAGACGATCGAGCACAACCGCGACAAGATGGTCGGGGTCACGGTGTTCATCGGCAAGAAGCGCGGCAAC
GCCAGCACCTCGGACTTCTCGTCGAGCGCGCTGAAGGACACGGTCGCGGCCGCCTACAACATCGCGCGCTTCACGGCCGA
GGACGATGCGGCGGGCCTGGCCGAAGCCGAGCTGCTGGAGACGGCGCCGCGCGACCTCGATCTCTACCACCCGTGGGCGC
TGAGCGCCGACGAGGCCGTCGAGATCGCGCGCCGCGCGGAGGACGCCGCGTTCGCGGTCAGCCCGCAGATCCGCAATTCC
GAAGGCGCGAGCGTGTCGGCGCAGCATTCGCAGTTCGTGCTGGCCACCTCGCGCGGCTTCCTGGGCGGCTACCCGTATTC
GCGTCACTACGTGGCCTGCGCGCCGATCGCCGGCAGCGGCCGCAACATGCAGCGTGACGATTGGTACAGCTCGAAGCGCA
ACGCCGCCGAGCTCGCCAGCCCCGAGGCGGTCGGCCGTTACGCGGCCGAGCGCGCGCTGGCGCGCATGGGTGCGCGCCGG
CTCGACACGCGCAAGGTGCCGGTGCTGTTCGAGGCGCCGCTCGCGGCGGGCCTGCTCGGCGCCTTCGTGCAGGCCACCAG
CGGCGGCGCGCTCTACCGCAAGACCACCTTCCTCGTCGACAGCCTCGGCAAGCCGGTGTTCGCGCCGCACGTGCAGGTGC
TCGAGGATCCGCACGTGCCGCGCGCGATGGGCAGCGCGCCGTTCGACGAGGAAGGCGTGCGCACGCAGGCGCGCAGCGTG
GTGAAGGACGGCGTGGTGGAAGGCTATTTCCTGTCGACCTATTCGGCGCGCAAGCTCAAGACGCAGACCACCGGCAACGC
GGGCGGCTCGCACAACCTCGCGCTGCGCAGCACCGGCACGAAGGCGGGCGACGATTTCGACGCGATGCTGAAGAAGCTCG
GCACCGGCCTGCTGCTGACCGAGCTGATGGGGCAGGGCGTGAACTACGTGACGGGCGACTATTCGCGCGGCGCGGCGGGC
TTCTGGGTCGAGAACGGCGTGATCCAGTACCCGGTGGAAGAGATCACGGTGGCGAGCACGCTGCAGGAGATGTTCCGCCA
CATCGTCGCGATCGGCGCCGACACGATCGTGCGCGGCACCAAGGAAACCGGCTCGGTGCTGATCGAGCAGATGACGATCG
CCGGGCAGTGA

Upstream 100 bases:

>100_bases
CGGCATGGGTAGGTGGCGGCGGCCGGACCTTGTTATGATCGCGGGATACGCCAATTTCGAAACAAGCGGGTGCCCGCGCC
CGCCACCGGATATCACGACG

Downstream 100 bases:

>100_bases
GCGGCGCGCGGGCTTGCCCCGCGCCGGGCTGAAGCAGGAAGCGCCGCGCGAAGCGGCGCTTTTTTTTATCGGCGCGGTGG
GGGCGCAGGCAGGCAGCGGG

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MPAHIDAPARHFPHTQEQLKEIASDILRHAKSLGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGN
ASTSDFSSSALKDTVAAAYNIARFTAEDDAAGLAEAELLETAPRDLDLYHPWALSADEAVEIARRAEDAAFAVSPQIRNS
EGASVSAQHSQFVLATSRGFLGGYPYSRHYVACAPIAGSGRNMQRDDWYSSKRNAAELASPEAVGRYAAERALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVLEDPHVPRAMGSAPFDEEGVRTQARSV
VKDGVVEGYFLSTYSARKLKTQTTGNAGGSHNLALRSTGTKAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGVIQYPVEEITVASTLQEMFRHIVAIGADTIVRGTKETGSVLIEQMTIAGQ

Sequences:

>Translated_456_residues
MPAHIDAPARHFPHTQEQLKEIASDILRHAKSLGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGN
ASTSDFSSSALKDTVAAAYNIARFTAEDDAAGLAEAELLETAPRDLDLYHPWALSADEAVEIARRAEDAAFAVSPQIRNS
EGASVSAQHSQFVLATSRGFLGGYPYSRHYVACAPIAGSGRNMQRDDWYSSKRNAAELASPEAVGRYAAERALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVLEDPHVPRAMGSAPFDEEGVRTQARSV
VKDGVVEGYFLSTYSARKLKTQTTGNAGGSHNLALRSTGTKAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGVIQYPVEEITVASTLQEMFRHIVAIGADTIVRGTKETGSVLIEQMTIAGQ
>Mature_455_residues
PAHIDAPARHFPHTQEQLKEIASDILRHAKSLGATDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGKKRGNA
STSDFSSSALKDTVAAAYNIARFTAEDDAAGLAEAELLETAPRDLDLYHPWALSADEAVEIARRAEDAAFAVSPQIRNSE
GASVSAQHSQFVLATSRGFLGGYPYSRHYVACAPIAGSGRNMQRDDWYSSKRNAAELASPEAVGRYAAERALARMGARRL
DTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVLEDPHVPRAMGSAPFDEEGVRTQARSVV
KDGVVEGYFLSTYSARKLKTQTTGNAGGSHNLALRSTGTKAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGF
WVENGVIQYPVEEITVASTLQEMFRHIVAIGADTIVRGTKETGSVLIEQMTIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=46.9248291571754, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48787; Mature: 48656

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHIDAPARHFPHTQEQLKEIASDILRHAKSLGATDAATEISEGDGLSVSVRRGEVETI
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGKKRGNASTSDFSSSALKDTVAAAYNIARFTAEDDAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
TAPRDLDLYHPWALSADEAVEIARRAEDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEEEEECCCC
LGGYPYSRHYVACAPIAGSGRNMQRDDWYSSKRNAAELASPEAVGRYAAERALARMGARR
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVLEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
RAMGSAPFDEEGVRTQARSVVKDGVVEGYFLSTYSARKLKTQTTGNAGGSHNLALRSTGT
HHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHEEECCCCCCCCCCCEEEEECCC
KAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGVIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADTIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAHIDAPARHFPHTQEQLKEIASDILRHAKSLGATDAATEISEGDGLSVSVRRGEVETI
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGKKRGNASTSDFSSSALKDTVAAAYNIARFTAEDDAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
TAPRDLDLYHPWALSADEAVEIARRAEDAAFAVSPQIRNSEGASVSAQHSQFVLATSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCCEEEEEECCCC
LGGYPYSRHYVACAPIAGSGRNMQRDDWYSSKRNAAELASPEAVGRYAAERALARMGARR
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVLEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHCCCCCC
RAMGSAPFDEEGVRTQARSVVKDGVVEGYFLSTYSARKLKTQTTGNAGGSHNLALRSTGT
HHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHEEECCCCCCCCCCCEEEEECCC
KAGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGVIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADTIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]