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The map label for this gene is metE [H]

Identifier: 238026499

GI number: 238026499

Start: 908241

End: 910517

Strand: Direct

Name: metE [H]

Synonym: bglu_1g08430

Alternate gene names: 238026499

Gene position: 908241-910517 (Clockwise)

Preceding gene: 238026493

Following gene: 238026501

Centisome position: 23.25

GC content: 73.03

Gene sequence:

>2277_bases
ATGAGCCGGGCGCATGTATTGGGATTCCCTCGTATCGGCGCGCAGCGTGAGGCGGCGCACGCGCTGGAGCACTATTGGCG
AGATGGCCGGTCGGCGCACGGCCTGCGCGTGCTCGCCGAAACCGGACGCGCGGTGCGTGCGGAGAACTGGCGGATGCAGC
GCGCCGCGGGCCTCGATCTGGTGACGGCCGGCGACTTCGCATGGGGCGATCACGTGCTGACCACGCTCGCGCATCTGGGC
GGGCTGCCGCGCCGCTTCGGCGCCGATCCCGAGCGGCTGACGCTCGACGCGCTGTTCGACGCGCTGCGCGGCACCGGCTC
GCGGCCGGCGATGGAAACCGCGGCCTGGTTCGATACGAACTATCGTTACGTGGTGCCGGAATTCGGTCCCGATACCGCGT
TCGGGCCCGGTGTCGGCTGGCTGTTCGACGAGGTGGCGGAGGCGCAGGCGCTCGGCCATTCGGTCAAGGTCGCGCTGGTG
GGGCCGCTCACGCTGCTCTGGCTCGGCCGTTCGCGCGGCGGGCTCGGCAACCGGCTGGCGCTGCTGCCCGCGCTGCTGGC
CGCGTACCGCGCGCTGCTCGCGCGGCTGGCCGCGCAGCACGTCGAATGGGTGCAGATCGACGAGCCGGTGCTTGCGCTCG
AACTGCCCGAGGCGTGGCTCGATGCGCTGCAGCCGGCCTGCGCGGCGCTGGCCGACGGCGCGCCGAAGCTGCTGCTGACC
ACCGGCTTCGGCGACGTCTCGGCGCTGGCCGCGCGCCTGAAGGCGCTGCCGGTGGCGGGCCTGCACCTCGACCTGGCGCG
CGCCGACGCGCAGCTCGACGCCTTCCTCGCCGGCTGGCCGGCCGACAAGGTGCTCTCGTGCGGCGTCGTCGACGGCCGCA
ACGTCTGGCGCAACGATCTCGACCGTTCGCATGCGCGCCTCGCGGCGGCCCGCGCCGCGCTCGGCGACCGGCTGTGGGTG
GCCACCAGCTGCTCGCTGCTGCACGTGCCGGTCGATTTGCGCGCCGAGACGAAGCTCGACGAGGAGCTGAAATCATGGCT
CGCGTTTGCGGCCCAGAAGGCGCGCGAGGTGGCGCTGCTGCGCGACGCGCTGGTGCGGGGCCGTGAGGCGGTGGCCGGCG
CGCTCGACGAGGCGCGCCGTGCCGCCGAGGCGCGCGCCGCGTCGCCGCGCGTGCGCAACGTGCTGGTCGAGCGGCGCGTC
GCGGCGCTCGGCGAAGCCGACCTGCGCCGCGGCTCGCCCTATGCGGTGCGCGCGGCCCGCCAGCAGGCGCGCTTCGCGCT
GCCGCCGCTGCCGACCACCACGATCGGCTCGTTTCCATGCACGGACGGCCTGCGCGACACGCGCGAGGCGTTTCGGCGCG
GCGCGCTCGATCACCTCGGCTATCTGGAGGCGATGCGCGAGCAGATCCGCTACGCGATCGACAAGCAGCTCCATTACGGG
CTCGACGTCCTGGTGCACGGCGAGGCGGAGCGCGACGACATGGTGGAGTTCTTCGCCGGGCAGCTGTGGGGCTGCGCGGT
GACGGAGGCGGGCTGGGTGCAGCGCGGCCCTGCCGCGTGCGTGAAGCCGCCGATCGTCTATGGCGACGTCTACCTGCCCG
AGCCGATGACGGTCGGCTGGACGCAGTACGCGCAGAGCTTGACCGACAAGCCGGTGAAGGGCCTGCTGACCGGGCCCATG
ACGCTGCTGCAGCGTTCGTTCGTGCGTGACGACCTGCCGCGCGAGACGGTCGCGCTGCAGCTTGCGCTGGCGCTGCGCCA
GGAAACGATCGAGCTGGAGAAGGCCGGCATCGGCATGATCCAGATCGACGAGCCGGCGCTGCGCGACGGCCTGCCGCTCG
CGGCTGCCGGGCGCGCGGCGTTCCTCGACCTGGCGGTGCGCGCGTTCCGGGTGGCCTCGTCGGGGGTCGCGGACGACACG
CAGATCCACACCCATATCTGCGATGCCTCGCTCGACGACGTGCTGCCGGCGATCGCCGCGCTCGATGCCGACGTGATCTC
GATCGAGACCACGCATTCGAACGGCACGCTGCTCGACACCGGCTATCCGAATGCGATCGGCCCGGGCGTCTACGACATCC
ATTCGCCGCGCGTGCCCGACGAGGACGAGATCGCGGCACGGCTCGCGGCCGCGCTCGAACGGGTGCCGGCCACGCGCCTG
TGGGTCAATCCGGACTGCGGGCTGCGCGAACGCGAATGGGCGCAGGTGGACGCGGCGCTGCACGCGCTGGTCGGCGCGGC
ACGACGCCTGCGCGAGCAGGTGGCCGCGTTCGGTTGA

Upstream 100 bases:

>100_bases
ATTTTTCATGTTCAACTCAAGAAAATTTCGTTTGAGTAATGATAAGCGAGATCCGATACTGCAGCCCATATCGACTGCAG
CCCATATCTGGAGGACACCA

Downstream 100 bases:

>100_bases
GCACGCGGCGTCCCGCGTGCCGCCGCCATGAAAAAACGGCCGGCGCCGCTCAAGCGGCGCCGGCCGTCTGGTCGACGCGG
CCCGGCCGGGTCGGCTGGGC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSRAHVLGFPRIGAQREAAHALEHYWRDGRSAHGLRVLAETGRAVRAENWRMQRAAGLDLVTAGDFAWGDHVLTTLAHLG
GLPRRFGADPERLTLDALFDALRGTGSRPAMETAAWFDTNYRYVVPEFGPDTAFGPGVGWLFDEVAEAQALGHSVKVALV
GPLTLLWLGRSRGGLGNRLALLPALLAAYRALLARLAAQHVEWVQIDEPVLALELPEAWLDALQPACAALADGAPKLLLT
TGFGDVSALAARLKALPVAGLHLDLARADAQLDAFLAGWPADKVLSCGVVDGRNVWRNDLDRSHARLAAARAALGDRLWV
ATSCSLLHVPVDLRAETKLDEELKSWLAFAAQKAREVALLRDALVRGREAVAGALDEARRAAEARAASPRVRNVLVERRV
AALGEADLRRGSPYAVRAARQQARFALPPLPTTTIGSFPCTDGLRDTREAFRRGALDHLGYLEAMREQIRYAIDKQLHYG
LDVLVHGEAERDDMVEFFAGQLWGCAVTEAGWVQRGPAACVKPPIVYGDVYLPEPMTVGWTQYAQSLTDKPVKGLLTGPM
TLLQRSFVRDDLPRETVALQLALALRQETIELEKAGIGMIQIDEPALRDGLPLAAAGRAAFLDLAVRAFRVASSGVADDT
QIHTHICDASLDDVLPAIAALDADVISIETTHSNGTLLDTGYPNAIGPGVYDIHSPRVPDEDEIAARLAAALERVPATRL
WVNPDCGLREREWAQVDAALHALVGAARRLREQVAAFG

Sequences:

>Translated_758_residues
MSRAHVLGFPRIGAQREAAHALEHYWRDGRSAHGLRVLAETGRAVRAENWRMQRAAGLDLVTAGDFAWGDHVLTTLAHLG
GLPRRFGADPERLTLDALFDALRGTGSRPAMETAAWFDTNYRYVVPEFGPDTAFGPGVGWLFDEVAEAQALGHSVKVALV
GPLTLLWLGRSRGGLGNRLALLPALLAAYRALLARLAAQHVEWVQIDEPVLALELPEAWLDALQPACAALADGAPKLLLT
TGFGDVSALAARLKALPVAGLHLDLARADAQLDAFLAGWPADKVLSCGVVDGRNVWRNDLDRSHARLAAARAALGDRLWV
ATSCSLLHVPVDLRAETKLDEELKSWLAFAAQKAREVALLRDALVRGREAVAGALDEARRAAEARAASPRVRNVLVERRV
AALGEADLRRGSPYAVRAARQQARFALPPLPTTTIGSFPCTDGLRDTREAFRRGALDHLGYLEAMREQIRYAIDKQLHYG
LDVLVHGEAERDDMVEFFAGQLWGCAVTEAGWVQRGPAACVKPPIVYGDVYLPEPMTVGWTQYAQSLTDKPVKGLLTGPM
TLLQRSFVRDDLPRETVALQLALALRQETIELEKAGIGMIQIDEPALRDGLPLAAAGRAAFLDLAVRAFRVASSGVADDT
QIHTHICDASLDDVLPAIAALDADVISIETTHSNGTLLDTGYPNAIGPGVYDIHSPRVPDEDEIAARLAAALERVPATRL
WVNPDCGLREREWAQVDAALHALVGAARRLREQVAAFG
>Mature_757_residues
SRAHVLGFPRIGAQREAAHALEHYWRDGRSAHGLRVLAETGRAVRAENWRMQRAAGLDLVTAGDFAWGDHVLTTLAHLGG
LPRRFGADPERLTLDALFDALRGTGSRPAMETAAWFDTNYRYVVPEFGPDTAFGPGVGWLFDEVAEAQALGHSVKVALVG
PLTLLWLGRSRGGLGNRLALLPALLAAYRALLARLAAQHVEWVQIDEPVLALELPEAWLDALQPACAALADGAPKLLLTT
GFGDVSALAARLKALPVAGLHLDLARADAQLDAFLAGWPADKVLSCGVVDGRNVWRNDLDRSHARLAAARAALGDRLWVA
TSCSLLHVPVDLRAETKLDEELKSWLAFAAQKAREVALLRDALVRGREAVAGALDEARRAAEARAASPRVRNVLVERRVA
ALGEADLRRGSPYAVRAARQQARFALPPLPTTTIGSFPCTDGLRDTREAFRRGALDHLGYLEAMREQIRYAIDKQLHYGL
DVLVHGEAERDDMVEFFAGQLWGCAVTEAGWVQRGPAACVKPPIVYGDVYLPEPMTVGWTQYAQSLTDKPVKGLLTGPMT
LLQRSFVRDDLPRETVALQLALALRQETIELEKAGIGMIQIDEPALRDGLPLAAAGRAAFLDLAVRAFRVASSGVADDTQ
IHTHICDASLDDVLPAIAALDADVISIETTHSNGTLLDTGYPNAIGPGVYDIHSPRVPDEDEIAARLAAALERVPATRLW
VNPDCGLREREWAQVDAALHALVGAARRLREQVAAFG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=756, Percent_Identity=52.1164021164021, Blast_Score=716, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=772, Percent_Identity=43.0051813471503, Blast_Score=587, Evalue=1e-168,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 82045; Mature: 81913

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAHVLGFPRIGAQREAAHALEHYWRDGRSAHGLRVLAETGRAVRAENWRMQRAAGLDL
CCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCCCHHHHHCCCEE
VTAGDFAWGDHVLTTLAHLGGLPRRFGADPERLTLDALFDALRGTGSRPAMETAAWFDTN
EECCCCCCHHHHHHHHHHHCCCCHHHCCCHHHEEHHHHHHHHHCCCCCCCHHHHHHCCCC
YRYVVPEFGPDTAFGPGVGWLFDEVAEAQALGHSVKVALVGPLTLLWLGRSRGGLGNRLA
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEHEEECCCCCCCCHHHH
LLPALLAAYRALLARLAAQHVEWVQIDEPVLALELPEAWLDALQPACAALADGAPKLLLT
HHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEE
TGFGDVSALAARLKALPVAGLHLDLARADAQLDAFLAGWPADKVLSCGVVDGRNVWRNDL
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHH
DRSHARLAAARAALGDRLWVATSCSLLHVPVDLRAETKLDEELKSWLAFAAQKAREVALL
HHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDALVRGREAVAGALDEARRAAEARAASPRVRNVLVERRVAALGEADLRRGSPYAVRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHH
QQARFALPPLPTTTIGSFPCTDGLRDTREAFRRGALDHLGYLEAMREQIRYAIDKQLHYG
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEAERDDMVEFFAGQLWGCAVTEAGWVQRGPAACVKPPIVYGDVYLPEPMTVGW
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCEECCCCCCCCH
TQYAQSLTDKPVKGLLTGPMTLLQRSFVRDDLPRETVALQLALALRQETIELEKAGIGMI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
QIDEPALRDGLPLAAAGRAAFLDLAVRAFRVASSGVADDTQIHTHICDASLDDVLPAIAA
EECCCHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHH
LDADVISIETTHSNGTLLDTGYPNAIGPGVYDIHSPRVPDEDEIAARLAAALERVPATRL
CCCCEEEEEEECCCCCEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
WVNPDCGLREREWAQVDAALHALVGAARRLREQVAAFG
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRAHVLGFPRIGAQREAAHALEHYWRDGRSAHGLRVLAETGRAVRAENWRMQRAAGLDL
CCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCCCHHHHHCCCEE
VTAGDFAWGDHVLTTLAHLGGLPRRFGADPERLTLDALFDALRGTGSRPAMETAAWFDTN
EECCCCCCHHHHHHHHHHHCCCCHHHCCCHHHEEHHHHHHHHHCCCCCCCHHHHHHCCCC
YRYVVPEFGPDTAFGPGVGWLFDEVAEAQALGHSVKVALVGPLTLLWLGRSRGGLGNRLA
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEHEEECCCCCCCCHHHH
LLPALLAAYRALLARLAAQHVEWVQIDEPVLALELPEAWLDALQPACAALADGAPKLLLT
HHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEE
TGFGDVSALAARLKALPVAGLHLDLARADAQLDAFLAGWPADKVLSCGVVDGRNVWRNDL
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHH
DRSHARLAAARAALGDRLWVATSCSLLHVPVDLRAETKLDEELKSWLAFAAQKAREVALL
HHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDALVRGREAVAGALDEARRAAEARAASPRVRNVLVERRVAALGEADLRRGSPYAVRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHH
QQARFALPPLPTTTIGSFPCTDGLRDTREAFRRGALDHLGYLEAMREQIRYAIDKQLHYG
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
LDVLVHGEAERDDMVEFFAGQLWGCAVTEAGWVQRGPAACVKPPIVYGDVYLPEPMTVGW
CEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCEECCCCCCCCH
TQYAQSLTDKPVKGLLTGPMTLLQRSFVRDDLPRETVALQLALALRQETIELEKAGIGMI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
QIDEPALRDGLPLAAAGRAAFLDLAVRAFRVASSGVADDTQIHTHICDASLDDVLPAIAA
EECCCHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHH
LDADVISIETTHSNGTLLDTGYPNAIGPGVYDIHSPRVPDEDEIAARLAAALERVPATRL
CCCCEEEEEEECCCCCEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
WVNPDCGLREREWAQVDAALHALVGAARRLREQVAAFG
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA