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The map label for this gene is waaA [H]

Identifier: 238026416

GI number: 238026416

Start: 815574

End: 816965

Strand: Direct

Name: waaA [H]

Synonym: bglu_1g07510

Alternate gene names: 238026416

Gene position: 815574-816965 (Clockwise)

Preceding gene: 238026415

Following gene: 238026424

Centisome position: 20.88

GC content: 75.93

Gene sequence:

>1392_bases
ATGTTGAGAGCGGTGTATCGCGCGCTTTGGTGGATCGTCGCGCCCGCTGCGGTAGTGCGCCTGTTCTGGCGGTCGCGCCG
CGAGCGCGGCTATCGCGAGCATATCGGCGAGCGCTTCGGCCATCTCGCGCCGGCCATGCGCGCCGCGCGTGGCCCCGACG
ACAGCGCGCCGCTGATCTGGGTCCACGCGGTGTCGGTGGGCGAAACGCGCGCCGCGCAACCGCTGATCAAGGCGCTGCTG
GCCGCGCGGCCCGACGCGAAGCTGCTCCTCACGCACATGACGCCGAGCGGGCGCGCGACCGGCGAGCAGCTGTTCGGCGC
GCGCGTGCTGCGCGCCTATCTGCCCTACGACATGCCGCATCTGGTGCGCCGTTTCCTGCGCGCCTGGCGTCCCACGCTCG
GGCTCGTGATGGAGACCGAGGTCTGGCCGACGCTGATCGACGAATGCCGGCGCGCCGCGGTGCCGCTGGTGCTGACCAAC
GCGCGCATGTCGGCGCGCTCGTTCCGGCGCGCCGCGCGGTTCGGGCCGGCCGCGCGCGAGGTGTTCGGCGGCTTCTCGCG
CGTGCTCGCGCAGAGCCCTGCCGATGCCGAGCGGCTGATGGCGCTCGGCGCGCGCAACGTGGTGGTGCTCGGCAACCTGA
AGTTCGACATGAGCACACCGCCCGAGCTGGTTGCGCGCGGTCGCGCATGGCGCGAGGCGATCGGCCGCCGGCCCGTGTGG
GTGGCGGCCAGCACGCGCGAGGGCGAGGAGGCGCTGGTGCTGCGCGCGTTCGCGGCGCTGAAGACGCCCGGCGCGCTGCT
GATCCTGGTGCCGCGCCATCCGCAGCGCTTCGACGAGGTGGCCGCGCTGGCCGGGCGCGAGGGGCTCGCGCTCGCACGGC
GCTCGACGCACTGGGCGCCCGGCGCGGCCGCGGCCGCCGGCCTCACGGCCGCGCCGCTCGATGCCGGCGTCGCGGTGTTG
CTGGGCGATTCGATGGGTGAGCTGGGCGCCTACTACGCGGCTGCCGACGCGGCCTTCATCGGCGGCAGCCTGCTGCCGCT
CGGCGGCCAGAACCTGATCGAGGCCTGCGCGGTGGGCGTGCCGGTGTTGATCGGGCCGCACATGTTCAACTTCACGCAGG
CCACCGCCGACGCGGTGGCGGCTGGCGCCGCCGCGCAGGTGCGCGATCCGGCCGAACTGGCCACGGCGCTCGACGCGCTG
TTCGCCGATCCGGCGCGGCGCACCGCGATGGGCGCGGCCGGCGCCGCGTTCGCGGCGCGCCATCGCGGCGCGACCGCCCG
CACCGTCGACGTGCTGACCGCGCTGCTGCCGCCACCGGGCGCGGCCGCGCGCGGCGGCGAGGGCGCAGGCGAGCACGACG
CGAGCGACGGCGGCGAGCCCGAGCCGGCTTGA

Upstream 100 bases:

>100_bases
CGGCCCGAATGCGCGCCGCTCGGCATCCTCGAACGCCTGCAGCAGCGCGCGCTCGTCTGAGCGCGGCAAACCGAATGGAA
ATCACGATGATGATGGCGTC

Downstream 100 bases:

>100_bases
GCCCGGAACCCGCGGCGCGGCCTCGGCCGCCGGCCGCCGCGCGTGCCCGGCTTGCTTGGCGGGCCAGGCAGGGCCGGCAC
CGTCAGGCCGCCAGCAATCC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLRAVYRALWWIVAPAAVVRLFWRSRRERGYREHIGERFGHLAPAMRAARGPDDSAPLIWVHAVSVGETRAAQPLIKALL
AARPDAKLLLTHMTPSGRATGEQLFGARVLRAYLPYDMPHLVRRFLRAWRPTLGLVMETEVWPTLIDECRRAAVPLVLTN
ARMSARSFRRAARFGPAAREVFGGFSRVLAQSPADAERLMALGARNVVVLGNLKFDMSTPPELVARGRAWREAIGRRPVW
VAASTREGEEALVLRAFAALKTPGALLILVPRHPQRFDEVAALAGREGLALARRSTHWAPGAAAAAGLTAAPLDAGVAVL
LGDSMGELGAYYAAADAAFIGGSLLPLGGQNLIEACAVGVPVLIGPHMFNFTQATADAVAAGAAAQVRDPAELATALDAL
FADPARRTAMGAAGAAFAARHRGATARTVDVLTALLPPPGAAARGGEGAGEHDASDGGEPEPA

Sequences:

>Translated_463_residues
MLRAVYRALWWIVAPAAVVRLFWRSRRERGYREHIGERFGHLAPAMRAARGPDDSAPLIWVHAVSVGETRAAQPLIKALL
AARPDAKLLLTHMTPSGRATGEQLFGARVLRAYLPYDMPHLVRRFLRAWRPTLGLVMETEVWPTLIDECRRAAVPLVLTN
ARMSARSFRRAARFGPAAREVFGGFSRVLAQSPADAERLMALGARNVVVLGNLKFDMSTPPELVARGRAWREAIGRRPVW
VAASTREGEEALVLRAFAALKTPGALLILVPRHPQRFDEVAALAGREGLALARRSTHWAPGAAAAAGLTAAPLDAGVAVL
LGDSMGELGAYYAAADAAFIGGSLLPLGGQNLIEACAVGVPVLIGPHMFNFTQATADAVAAGAAAQVRDPAELATALDAL
FADPARRTAMGAAGAAFAARHRGATARTVDVLTALLPPPGAAARGGEGAGEHDASDGGEPEPA
>Mature_463_residues
MLRAVYRALWWIVAPAAVVRLFWRSRRERGYREHIGERFGHLAPAMRAARGPDDSAPLIWVHAVSVGETRAAQPLIKALL
AARPDAKLLLTHMTPSGRATGEQLFGARVLRAYLPYDMPHLVRRFLRAWRPTLGLVMETEVWPTLIDECRRAAVPLVLTN
ARMSARSFRRAARFGPAAREVFGGFSRVLAQSPADAERLMALGARNVVVLGNLKFDMSTPPELVARGRAWREAIGRRPVW
VAASTREGEEALVLRAFAALKTPGALLILVPRHPQRFDEVAALAGREGLALARRSTHWAPGAAAAAGLTAAPLDAGVAVL
LGDSMGELGAYYAAADAAFIGGSLLPLGGQNLIEACAVGVPVLIGPHMFNFTQATADAVAAGAAAQVRDPAELATALDAL
FADPARRTAMGAAGAAFAARHRGATARTVDVLTALLPPPGAAARGGEGAGEHDASDGGEPEPA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=443, Percent_Identity=39.5033860045147, Blast_Score=294, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 48993; Mature: 48993

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAVYRALWWIVAPAAVVRLFWRSRRERGYREHIGERFGHLAPAMRAARGPDDSAPLIW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VHAVSVGETRAAQPLIKALLAARPDAKLLLTHMTPSGRATGEQLFGARVLRAYLPYDMPH
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHH
LVRRFLRAWRPTLGLVMETEVWPTLIDECRRAAVPLVLTNARMSARSFRRAARFGPAARE
HHHHHHHHHCCHHCEEEECHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHH
VFGGFSRVLAQSPADAERLMALGARNVVVLGNLKFDMSTPPELVARGRAWREAIGRRPVW
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHCCCCEE
VAASTREGEEALVLRAFAALKTPGALLILVPRHPQRFDEVAALAGREGLALARRSTHWAP
EEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCC
GAAAAAGLTAAPLDAGVAVLLGDSMGELGAYYAAADAAFIGGSLLPLGGQNLIEACAVGV
CHHHHCCCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
PVLIGPHMFNFTQATADAVAAGAAAQVRDPAELATALDALFADPARRTAMGAAGAAFAAR
CCEECCCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
HRGATARTVDVLTALLPPPGAAARGGEGAGEHDASDGGEPEPA
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRAVYRALWWIVAPAAVVRLFWRSRRERGYREHIGERFGHLAPAMRAARGPDDSAPLIW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VHAVSVGETRAAQPLIKALLAARPDAKLLLTHMTPSGRATGEQLFGARVLRAYLPYDMPH
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHH
LVRRFLRAWRPTLGLVMETEVWPTLIDECRRAAVPLVLTNARMSARSFRRAARFGPAARE
HHHHHHHHHCCHHCEEEECHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHH
VFGGFSRVLAQSPADAERLMALGARNVVVLGNLKFDMSTPPELVARGRAWREAIGRRPVW
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHCCHHHHHHHCCCCEE
VAASTREGEEALVLRAFAALKTPGALLILVPRHPQRFDEVAALAGREGLALARRSTHWAP
EEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCCCC
GAAAAAGLTAAPLDAGVAVLLGDSMGELGAYYAAADAAFIGGSLLPLGGQNLIEACAVGV
CHHHHCCCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
PVLIGPHMFNFTQATADAVAAGAAAQVRDPAELATALDALFADPARRTAMGAAGAAFAAR
CCEECCCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHH
HRGATARTVDVLTALLPPPGAAARGGEGAGEHDASDGGEPEPA
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]