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The map label for this gene is yafC [C]

Identifier: 238026362

GI number: 238026362

Start: 749383

End: 750357

Strand: Reverse

Name: yafC [C]

Synonym: bglu_1g06960

Alternate gene names: 238026362

Gene position: 750357-749383 (Counterclockwise)

Preceding gene: 238026364

Following gene: 238026361

Centisome position: 19.21

GC content: 69.13

Gene sequence:

>975_bases
ATGAACCAGATCCAAACCATGAGAGTGTTCGTCTGCGTCGCCGAACTGCAGAGCTTTCGACAAGCCGCGCGCAAGCTCGG
CGTATCGAACGCTCTCGTCACGCGATCGATCGCGATGCTGGAAACCCACCTGAACACACGCCTGATCCACCGCACCACGC
GCAACCTGTCGCTGACCGAGGCCGGCACCCGCTATCTCGATGGCTGCCGCGCCCTGCTCGAAGAGTTCGATCACCTCGAG
GCCTCGGTCGCGCACACCATCCGCGAGCCGGTGGGCACGCTGCGCATCGCCGTGTCGGGGGCGCTCTCGCCGCAACGGCT
GACGCCGCTGGTCGACGGCTTCCGCCGCCAGTATCCGAAGGTGCGCGTGCAACTGACCATCGCCGACGGGCCGATCGACG
GGCTCGACACCTCCTACGACGCGGCGATCGTGGCCGGCCGCCGGATCGACGACGGCCCCGCGCTCGTGAGCCATGCGCTC
GCGCCCGACCCGTTCGTGGCGATCGCCTCGCCGGCCTATATCGAACAGCGCGGCGAGCCGCACCGCCCCGACGAACTCGC
GCGGCACGCGGCCATCACGCTGCCACTCGACACGCACGGCTTCGCATGGCGCTTCGTCGATGCCGGCCGCTTCGCGCACC
TCGTCACGCTGCAGCCGTCCTACACGATCAACGACGCCTGGCTGGTGCGCGCCGCCGTGATGGCCGGCTCGGGCATCGCG
ATCCTGCCGGAGAGCTTCGTGGCCGACGCGATCTCGCGCGGCGAACTGGTGCGGCTGCTGGCCGACTACCGGATCGACGA
TCCCGACGCGCAGCTCGCGGTGGTCTATCCGAACCGCCAGTTCGTGCCGGCGCGCACGCGCAGCTTCGTCGAGCACGCGC
TCTATCACTTCGGCGCGCGCTCGAACGGCCGCTACGGCTACGTCCACGACACGCTCGGCGAACATGCCGAGGTCGTCACC
ACCGGCCTGCAATGA

Upstream 100 bases:

>100_bases
TATTACGATAATCGCCCCGTGCTGATCCGACAGCGCTTGACGGGACCATTCTCGACACGGCAATTACACAACCAAAATCA
TCCGAACGGGCCGACAAGCT

Downstream 100 bases:

>100_bases
CGCGGGCGGCGGCGGCCGGCGCCGTCGTCGAATGCGGCGAATAGCCGCACCCATGACCGCGGACCGCCCCGTCGCCAGCG
GCGACACGCGCCGCGGCGGT

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNQIQTMRVFVCVAELQSFRQAARKLGVSNALVTRSIAMLETHLNTRLIHRTTRNLSLTEAGTRYLDGCRALLEEFDHLE
ASVAHTIREPVGTLRIAVSGALSPQRLTPLVDGFRRQYPKVRVQLTIADGPIDGLDTSYDAAIVAGRRIDDGPALVSHAL
APDPFVAIASPAYIEQRGEPHRPDELARHAAITLPLDTHGFAWRFVDAGRFAHLVTLQPSYTINDAWLVRAAVMAGSGIA
ILPESFVADAISRGELVRLLADYRIDDPDAQLAVVYPNRQFVPARTRSFVEHALYHFGARSNGRYGYVHDTLGEHAEVVT
TGLQ

Sequences:

>Translated_324_residues
MNQIQTMRVFVCVAELQSFRQAARKLGVSNALVTRSIAMLETHLNTRLIHRTTRNLSLTEAGTRYLDGCRALLEEFDHLE
ASVAHTIREPVGTLRIAVSGALSPQRLTPLVDGFRRQYPKVRVQLTIADGPIDGLDTSYDAAIVAGRRIDDGPALVSHAL
APDPFVAIASPAYIEQRGEPHRPDELARHAAITLPLDTHGFAWRFVDAGRFAHLVTLQPSYTINDAWLVRAAVMAGSGIA
ILPESFVADAISRGELVRLLADYRIDDPDAQLAVVYPNRQFVPARTRSFVEHALYHFGARSNGRYGYVHDTLGEHAEVVT
TGLQ
>Mature_324_residues
MNQIQTMRVFVCVAELQSFRQAARKLGVSNALVTRSIAMLETHLNTRLIHRTTRNLSLTEAGTRYLDGCRALLEEFDHLE
ASVAHTIREPVGTLRIAVSGALSPQRLTPLVDGFRRQYPKVRVQLTIADGPIDGLDTSYDAAIVAGRRIDDGPALVSHAL
APDPFVAIASPAYIEQRGEPHRPDELARHAAITLPLDTHGFAWRFVDAGRFAHLVTLQPSYTINDAWLVRAAVMAGSGIA
ILPESFVADAISRGELVRLLADYRIDDPDAQLAVVYPNRQFVPARTRSFVEHALYHFGARSNGRYGYVHDTLGEHAEVVT
TGLQ

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=31.1036789297659, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789639, Length=306, Percent_Identity=30.3921568627451, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=29.4736842105263, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=30.6397306397306, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=30.1694915254237, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=28.135593220339, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=27.681660899654, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=25.4295532646048, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788748, Length=257, Percent_Identity=27.6264591439689, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=127, Percent_Identity=33.8582677165354, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=25.4980079681275, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=317, Percent_Identity=25.8675078864353, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=258, Percent_Identity=25.1937984496124, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=23.1578947368421, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786713, Length=279, Percent_Identity=25.089605734767, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35703; Mature: 35703

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIQTMRVFVCVAELQSFRQAARKLGVSNALVTRSIAMLETHLNTRLIHRTTRNLSLTE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
AGTRYLDGCRALLEEFDHLEASVAHTIREPVGTLRIAVSGALSPQRLTPLVDGFRRQYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCHHHHHHHHHHHHHCCCE
VRVQLTIADGPIDGLDTSYDAAIVAGRRIDDGPALVSHALAPDPFVAIASPAYIEQRGEP
EEEEEEEECCCCCCCCCCCCHHEEECCCCCCCHHHHHHCCCCCCEEEECCCHHHHHCCCC
HRPDELARHAAITLPLDTHGFAWRFVDAGRFAHLVTLQPSYTINDAWLVRAAVMAGSGIA
CCHHHHHHHEEEEEEECCCCCEEEEECCCCEEEEEEECCCCEECHHHHHHHHHHCCCCEE
ILPESFVADAISRGELVRLLADYRIDDPDAQLAVVYPNRQFVPARTRSFVEHALYHFGAR
ECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
SNGRYGYVHDTLGEHAEVVTTGLQ
CCCCEEEEEHHHCCCHHHHCCCCC
>Mature Secondary Structure
MNQIQTMRVFVCVAELQSFRQAARKLGVSNALVTRSIAMLETHLNTRLIHRTTRNLSLTE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
AGTRYLDGCRALLEEFDHLEASVAHTIREPVGTLRIAVSGALSPQRLTPLVDGFRRQYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCHHHHHHHHHHHHHCCCE
VRVQLTIADGPIDGLDTSYDAAIVAGRRIDDGPALVSHALAPDPFVAIASPAYIEQRGEP
EEEEEEEECCCCCCCCCCCCHHEEECCCCCCCHHHHHHCCCCCCEEEECCCHHHHHCCCC
HRPDELARHAAITLPLDTHGFAWRFVDAGRFAHLVTLQPSYTINDAWLVRAAVMAGSGIA
CCHHHHHHHEEEEEEECCCCCEEEEECCCCEEEEEEECCCCEECHHHHHHHHHHCCCCEE
ILPESFVADAISRGELVRLLADYRIDDPDAQLAVVYPNRQFVPARTRSFVEHALYHFGAR
ECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
SNGRYGYVHDTLGEHAEVVTTGLQ
CCCCEEEEEHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]