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The map label for this gene is miaA [H]

Identifier: 238026310

GI number: 238026310

Start: 686147

End: 687115

Strand: Reverse

Name: miaA [H]

Synonym: bglu_1g06440

Alternate gene names: 238026310

Gene position: 687115-686147 (Counterclockwise)

Preceding gene: 238026311

Following gene: 238026308

Centisome position: 17.59

GC content: 73.99

Gene sequence:

>969_bases
ATGAGCGGCGCACCCGAATCGCCCCGCGTCACCGTCGCCTGCCTGCTCGGCCCCACCGCCTCGGGCAAGACCGCCGCGGC
GCTGGCGCTGGCCGAGCGGCGGCCGGTGGAGATCGTCAGCGTCGATTCGGCGCTCGTCTATCGCGAGATGGATATCGGCA
CCGCGAAGCCGGAGCGCGCCGAACGCGAGCGCGTGGCGCATCATCTGATCGACATCCGCGACCCGCGCGAGGCCTATTCG
GCCGCCGAGTTCCGCGCCGACACGCTGCGCCTGCTGGGCGAGATCCTCGCGCGCGGACACACGCCGCTGCTGGCGGGCGG
GACCATGCTGTACTACAAGGCGCTGACGCAGGGGCTCAATGCGCTACCGGCCGCCGATCCGCAGGTGCGCGCGCGGCTCG
ACGCCGACGCCGCGCGCGACGGCTGGCCGGCGCTGCATGCGCGGCTCGCACGGGCCGACCCGGAAACCGCCGCGCGGCTG
GCGCCGAACGACGCGCAGCGCATCCAGCGTGCGCTCGAAATCCTCGAACTGAGCGGCCGGCCCATGTCGGCGCTGCTCGC
CGCACCGCCGCCAGCCTGCGACGAGGCGGCCCGCTACCGCTTCGTGCCGGTGGCGCTGGAGCCGTCCGAGCGCGCCGTGC
TGCATGCGCGGATCGCGGTGCGCTTCGACGCGATGCTCGCGGCCGGCTTCGTCGACGAGGTGGAACGGCTGCGCGGGCGC
GGCGACCTGCATCTGGGCCTGCCGTCGATGCGCTGCGTGGGCTACCGGCAGGCCTGGGAATATCTCGACGGCGCCACCGA
CCACGCCACGATGCGCGACAAGGGCATATTCGCCACGCGCCAGCTCTGCAAGCGGCAGCTGACCTGGCTGCGCTCGATGC
CGGAGCGCATCGTGGTGGACTGCTGCGCCGATGACGCGCCGCGGCGCGCCGTGGCCGCGCTTGAGCGCGTGCTCGACGCG
GCGCCCTGA

Upstream 100 bases:

>100_bases
AGATGGAGGCCACCGAGCGCGCCGACCAGTGCAACCACGGCCGGCCGACCTGGTACCAGCTCACGCTCGGCGATCTCGAC
CGCCTGTTCATGCGCGGCCA

Downstream 100 bases:

>100_bases
ACGCGTCCGCGCCAGGCAACAAAAAAGGCCGCGCTCCGCGGCCCTTCCTGCTGCACGGCACGAGCGCCGTGCCACCGATC
CTCCTGCTGCGCCAGCCTCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSGAPESPRVTVACLLGPTASGKTAAALALAERRPVEIVSVDSALVYREMDIGTAKPERAERERVAHHLIDIRDPREAYS
AAEFRADTLRLLGEILARGHTPLLAGGTMLYYKALTQGLNALPAADPQVRARLDADAARDGWPALHARLARADPETAARL
APNDAQRIQRALEILELSGRPMSALLAAPPPACDEAARYRFVPVALEPSERAVLHARIAVRFDAMLAAGFVDEVERLRGR
GDLHLGLPSMRCVGYRQAWEYLDGATDHATMRDKGIFATRQLCKRQLTWLRSMPERIVVDCCADDAPRRAVAALERVLDA
AP

Sequences:

>Translated_322_residues
MSGAPESPRVTVACLLGPTASGKTAAALALAERRPVEIVSVDSALVYREMDIGTAKPERAERERVAHHLIDIRDPREAYS
AAEFRADTLRLLGEILARGHTPLLAGGTMLYYKALTQGLNALPAADPQVRARLDADAARDGWPALHARLARADPETAARL
APNDAQRIQRALEILELSGRPMSALLAAPPPACDEAARYRFVPVALEPSERAVLHARIAVRFDAMLAAGFVDEVERLRGR
GDLHLGLPSMRCVGYRQAWEYLDGATDHATMRDKGIFATRQLCKRQLTWLRSMPERIVVDCCADDAPRRAVAALERVLDA
AP
>Mature_321_residues
SGAPESPRVTVACLLGPTASGKTAAALALAERRPVEIVSVDSALVYREMDIGTAKPERAERERVAHHLIDIRDPREAYSA
AEFRADTLRLLGEILARGHTPLLAGGTMLYYKALTQGLNALPAADPQVRARLDADAARDGWPALHARLARADPETAARLA
PNDAQRIQRALEILELSGRPMSALLAAPPPACDEAARYRFVPVALEPSERAVLHARIAVRFDAMLAAGFVDEVERLRGRG
DLHLGLPSMRCVGYRQAWEYLDGATDHATMRDKGIFATRQLCKRQLTWLRSMPERIVVDCCADDAPRRAVAALERVLDAA
P

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=29.5819935691318, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=53.7906137184115, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25144712, Length=277, Percent_Identity=27.4368231046931, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=26.530612244898, Blast_Score=119, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24649830, Length=241, Percent_Identity=31.9502074688797, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35129; Mature: 34998

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPESPRVTVACLLGPTASGKTAAALALAERRPVEIVSVDSALVYREMDIGTAKPERA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHEEECCCCCCCCHHH
ERERVAHHLIDIRDPREAYSAAEFRADTLRLLGEILARGHTPLLAGGTMLYYKALTQGLN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
ALPAADPQVRARLDADAARDGWPALHARLARADPETAARLAPNDAQRIQRALEILELSGR
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPPPACDEAARYRFVPVALEPSERAVLHARIAVRFDAMLAAGFVDEVERLRGR
CHHHHHCCCCCCCCHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDLHLGLPSMRCVGYRQAWEYLDGATDHATMRDKGIFATRQLCKRQLTWLRSMPERIVVD
CCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
CCADDAPRRAVAALERVLDAAP
HCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGAPESPRVTVACLLGPTASGKTAAALALAERRPVEIVSVDSALVYREMDIGTAKPERA
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHEEECCCCCCCCHHH
ERERVAHHLIDIRDPREAYSAAEFRADTLRLLGEILARGHTPLLAGGTMLYYKALTQGLN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
ALPAADPQVRARLDADAARDGWPALHARLARADPETAARLAPNDAQRIQRALEILELSGR
CCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
PMSALLAAPPPACDEAARYRFVPVALEPSERAVLHARIAVRFDAMLAAGFVDEVERLRGR
CHHHHHCCCCCCCCHHHCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDLHLGLPSMRCVGYRQAWEYLDGATDHATMRDKGIFATRQLCKRQLTWLRSMPERIVVD
CCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
CCADDAPRRAVAALERVLDAAP
HCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA