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The map label for this gene is yiaJ [H]

Identifier: 238026185

GI number: 238026185

Start: 544256

End: 545161

Strand: Direct

Name: yiaJ [H]

Synonym: bglu_1g05160

Alternate gene names: 238026185

Gene position: 544256-545161 (Clockwise)

Preceding gene: 238026184

Following gene: 238026186

Centisome position: 13.93

GC content: 71.3

Gene sequence:

>906_bases
ATGACCAAGACCTCGATTTCCGCCGCGCCGTCCAACGGCGCACCGGCGGCCGGCGCGTCGGCCGAACGGCTTGCCGACAG
CATCAGCGTGACCGGTTCGCCGCTCGACATCGCGGCGCAGCAGGCCGGCACCCAGACACTGCTGCGCGGCCTCGCGATCC
TCGAAGCGGTCGCGCTCGGCGCGCGCGACATGCGCGCGATCGGCGCCGCGCTCGGCACGACGCGCAGCACCACGCACCGG
CTCGTCAGCAGCCTGGTGCAGGCGCGCTACCTGCGTCAGATACAGGGCGGCTACCTGCTCGGCCCGAAGCTGATCGAGCT
CGGCACCATCGCGCTCGAACAGATGCCGCTCACGGCCGTGGCGCGCCCGCACCTGGAAGCGCTCGCCGAGGCGACGCTCG
ACACCATCCACCTCGGCATGCGCGACGGCGACGACGTGCTCTACATCGACAAGATCCCCGGCACGCGCGGCCTCGAAATG
CGCTCGCGGATCGGCCACCGGATGCCGCTCGCCTCGACCGGCATCGGCAAGGCGATGATGCTCGACCTCGAGCCCGACAC
CTGGGCCGCGCTGTTCGAGGCCTCGCGCCGCTCGCTGGCCGGCGTGAGCTTCAAGCCGGACCGCCGCCCCGATGCATCGA
CCTTTCTGCAGCGCATGGCGCACTACGCGGCGGGCGGCTACACGTTCGACCTCGAGGAGAACGAGGCGTCGATCCGCTGC
GTGGCCGCGCCGGTGCGCGATGCGTCGGGCGCGATCGTCGCCGCGGTGTCGGTGGCCAGCACGATTCCGTACATGCCGCT
CGACCGGATGGACGAACTGATTCCGATCGTGCAGCGCGAGGCGCGCGCGATCTCGGCGGAGCTCGGCTGGAGCGCGCCGC
AAACGACCCGCAGGATCAAGCGATGA

Upstream 100 bases:

>100_bases
GACGCCGATCCAGCAGTCTGCCGTTCGCCGTGCGTGCCGATTCTCGGGTGCCGCGTAAGCCACCGCTTCGCGACCGTTCG
TCCGCGACTCACGCTCCCTC

Downstream 100 bases:

>100_bases
CGATGTCCCGAACGGCTCCGAGCCCCGCCGCCCCCGCGTTGATCGCGATCGACTGGGGCACCACCTCGATGCGCGCGTTC
CTGTTCGCTGCCGACGGCAG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTKTSISAAPSNGAPAAGASAERLADSISVTGSPLDIAAQQAGTQTLLRGLAILEAVALGARDMRAIGAALGTTRSTTHR
LVSSLVQARYLRQIQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGMRDGDDVLYIDKIPGTRGLEM
RSRIGHRMPLASTGIGKAMMLDLEPDTWAALFEASRRSLAGVSFKPDRRPDASTFLQRMAHYAAGGYTFDLEENEASIRC
VAAPVRDASGAIVAAVSVASTIPYMPLDRMDELIPIVQREARAISAELGWSAPQTTRRIKR

Sequences:

>Translated_301_residues
MTKTSISAAPSNGAPAAGASAERLADSISVTGSPLDIAAQQAGTQTLLRGLAILEAVALGARDMRAIGAALGTTRSTTHR
LVSSLVQARYLRQIQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGMRDGDDVLYIDKIPGTRGLEM
RSRIGHRMPLASTGIGKAMMLDLEPDTWAALFEASRRSLAGVSFKPDRRPDASTFLQRMAHYAAGGYTFDLEENEASIRC
VAAPVRDASGAIVAAVSVASTIPYMPLDRMDELIPIVQREARAISAELGWSAPQTTRRIKR
>Mature_300_residues
TKTSISAAPSNGAPAAGASAERLADSISVTGSPLDIAAQQAGTQTLLRGLAILEAVALGARDMRAIGAALGTTRSTTHRL
VSSLVQARYLRQIQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGMRDGDDVLYIDKIPGTRGLEMR
SRIGHRMPLASTGIGKAMMLDLEPDTWAALFEASRRSLAGVSFKPDRRPDASTFLQRMAHYAAGGYTFDLEENEASIRCV
AAPVRDASGAIVAAVSVASTIPYMPLDRMDELIPIVQREARAISAELGWSAPQTTRRIKR

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=255, Percent_Identity=30.9803921568627, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788131, Length=266, Percent_Identity=32.3308270676692, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786468, Length=252, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788581, Length=249, Percent_Identity=31.7269076305221, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786715, Length=257, Percent_Identity=27.6264591439689, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790752, Length=256, Percent_Identity=23.828125, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32053; Mature: 31922

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTSISAAPSNGAPAAGASAERLADSISVTGSPLDIAAQQAGTQTLLRGLAILEAVALG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ARDMRAIGAALGTTRSTTHRLVSSLVQARYLRQIQGGYLLGPKLIELGTIALEQMPLTAV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHH
ARPHLEALAEATLDTIHLGMRDGDDVLYIDKIPGTRGLEMRSRIGHRMPLASTGIGKAMM
HHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEE
LDLEPDTWAALFEASRRSLAGVSFKPDRRPDASTFLQRMAHYAAGGYTFDLEENEASIRC
EEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEE
VAAPVRDASGAIVAAVSVASTIPYMPLDRMDELIPIVQREARAISAELGWSAPQTTRRIK
EEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
R
C
>Mature Secondary Structure 
TKTSISAAPSNGAPAAGASAERLADSISVTGSPLDIAAQQAGTQTLLRGLAILEAVALG
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ARDMRAIGAALGTTRSTTHRLVSSLVQARYLRQIQGGYLLGPKLIELGTIALEQMPLTAV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHH
ARPHLEALAEATLDTIHLGMRDGDDVLYIDKIPGTRGLEMRSRIGHRMPLASTGIGKAMM
HHHHHHHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEE
LDLEPDTWAALFEASRRSLAGVSFKPDRRPDASTFLQRMAHYAAGGYTFDLEENEASIRC
EEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEE
VAAPVRDASGAIVAAVSVASTIPYMPLDRMDELIPIVQREARAISAELGWSAPQTTRRIK
EEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]