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The map label for this gene is mpl [H]

Identifier: 238026180

GI number: 238026180

Start: 537912

End: 539324

Strand: Direct

Name: mpl [H]

Synonym: bglu_1g05110

Alternate gene names: 238026180

Gene position: 537912-539324 (Clockwise)

Preceding gene: 238026169

Following gene: 238026181

Centisome position: 13.77

GC content: 69.71

Gene sequence:

>1413_bases
ATGCATATCCACATTCTCGGCATCTGCGGCACCTTCATGGGCGGTCTGGCCGTGCTCGCGCGCGAGGCCGGCCATACCGT
GACCGGCTGCGACGCAGGCGTCTATCCGCCGATGAGCACGCAGCTCGAAGCGCAGGGGATACAACTGATCGAAGGCTACG
GCGCGGAGCAGATCGACCTGGCGCCGGACCTGTTCGTGATCGGCAACGTGGTCTCGCGCGGCAATCCGCTGATGGAGGCC
ATCCTCGATCGCGGGCTGCCCTACGTATCCGGCCCGCAATGGCTCGGCGAACACGTGCTCGCCGGCAAATGGGTGCTGGC
CGTGGCCGGCACCCACGGCAAGACCACCACCAGTTCGATGCTGGCCTGGCTGCTCGAGGAGTCGGGGCTGAACCCCGGTT
TCCTGATCGGCGGCGTGCCGCTGAACTTCGGCGTCTCGGCGCGGCTGACCGAGTCGAGCTTCTTCGTGATCGAGGCCGAC
GAATACGACACGGCGTTCTTCGACAAGCGCTCGAAGTTCGTCCATTACCGGCCGCGCACCGCCGTACTGAACAATCTCGA
ATTCGATCACGCGGACATCTTCCCCGATCTCGCTGCGATCGAAACGCAATTCCACCATCTGGTGCGCACGGTGCCGGGCG
TCGGCCGGATCGTCGCGAACGGCCGCGAGGCCGCGCTCGACCGCGTGCTCGCGCGCGGCTGCTGGAGCGAGGTCGAGCGC
TTCGGCGTGGAGGGCGGCTGGCAGGCGCTGCCTGCCGAGGACGGCGTGCCGGTGGACGAGCGCTTTGCCGTCTACTGGCA
GGGCGCGCGCATGGGCGAGGTCGCCTGGAGCGTGCAGGGCGAGCACAACCGCCAGAACGCGCTGGCCGCGATCGCGGCGG
CGCGCTCGGTCGGCGTGCCGCCGGCGCAGGCCGCACGCGCGTTCGCGACGTTTCGCAACGTCAAGCGCCGCATGGAGATT
CGCGGCAGCGTGGACGGCGTGACCGTCTACGACGACTTCGCCCATCATCCGACCGCGATCGAAACCACCATCGCCGGGCT
TCGCACGCGCATCGGCCGCGAACACACGCGGATTCTCGCCGTGCTCGAGCCGCGCTCGAACACCATGAAGCTCGGCACCA
TGAAGGCGCAATTGCCGGCCAGCCTCGCCGATGCCGATCTGGTGTTCGGCTATGGCGCGGCCTCGGGACGCGACGCGCTC
GGCTGGAGCCTGGCCGAGGCGCTGGCGCCGCTCGGCGCGCGCGCGCAGGCGTTCGACGACCTGCATGCGCTGGTGAAGGC
GGTGGCGGCCGCCGCACGGCCCGGCGACCAGATCCTCGTGATGAGCAACGGCGGCTTCGGCGGGGTCCACCAGAAGCTGC
TCGACGCGCTGTCGGCGCGCGGTGCCGCCCACGGCGCGGAGGACCGCGCGTGA

Upstream 100 bases:

>100_bases
GCGGTGCTGCGTGGCTGCGGCAAGCGCGGCACCGCCGCGTGCCGCGGATCCGGCCCGCGATTTCGAATGGCGCCCGCGCG
GCGCGACACGACGGCTCTCT

Downstream 100 bases:

>100_bases
TTCTCTATCTGCACGGCTTTCGCTCGTCGCCGCGTTCCTACAAGTCGCGCCTGCTTGCCGAGCGGCTTGCCGCGCTCGGC
CGCGAGGCCGAATGGCGCTG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAEQIDLAPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEESGLNPGFLIGGVPLNFGVSARLTESSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVANGREAALDRVLARGCWSEVER
FGVEGGWQALPAEDGVPVDERFAVYWQGARMGEVAWSVQGEHNRQNALAAIAAARSVGVPPAQAARAFATFRNVKRRMEI
RGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGREHTRILAVLEPRSNTMKLGTMKAQLPASLADADLVFGYGAASGRDAL
GWSLAEALAPLGARAQAFDDLHALVKAVAAAARPGDQILVMSNGGFGGVHQKLLDALSARGAAHGAEDRA

Sequences:

>Translated_470_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAEQIDLAPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEESGLNPGFLIGGVPLNFGVSARLTESSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVANGREAALDRVLARGCWSEVER
FGVEGGWQALPAEDGVPVDERFAVYWQGARMGEVAWSVQGEHNRQNALAAIAAARSVGVPPAQAARAFATFRNVKRRMEI
RGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGREHTRILAVLEPRSNTMKLGTMKAQLPASLADADLVFGYGAASGRDAL
GWSLAEALAPLGARAQAFDDLHALVKAVAAAARPGDQILVMSNGGFGGVHQKLLDALSARGAAHGAEDRA
>Mature_470_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAEQIDLAPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEESGLNPGFLIGGVPLNFGVSARLTESSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVANGREAALDRVLARGCWSEVER
FGVEGGWQALPAEDGVPVDERFAVYWQGARMGEVAWSVQGEHNRQNALAAIAAARSVGVPPAQAARAFATFRNVKRRMEI
RGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGREHTRILAVLEPRSNTMKLGTMKAQLPASLADADLVFGYGAASGRDAL
GWSLAEALAPLGARAQAFDDLHALVKAVAAAARPGDQILVMSNGGFGGVHQKLLDALSARGAAHGAEDRA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=463, Percent_Identity=59.6112311015119, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786279, Length=348, Percent_Identity=29.0229885057471, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50194; Mature: 50194

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAEQIDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHCCHHHHHCCCCCEECC
APDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWLLEESGLNPGFLIGGVPLNFGVSARLTESSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVANGREAALDRVLARGCWSEVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
FGVEGGWQALPAEDGVPVDERFAVYWQGARMGEVAWSVQGEHNRQNALAAIAAARSVGVP
HCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
PAQAARAFATFRNVKRRMEIRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGREHTRILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VLEPRSNTMKLGTMKAQLPASLADADLVFGYGAASGRDALGWSLAEALAPLGARAQAFDD
EECCCCCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
LHALVKAVAAAARPGDQILVMSNGGFGGVHQKLLDALSARGAAHGAEDRA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAEQIDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHCCHHHHHCCCCCEECC
APDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWLLEESGLNPGFLIGGVPLNFGVSARLTESSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVANGREAALDRVLARGCWSEVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
FGVEGGWQALPAEDGVPVDERFAVYWQGARMGEVAWSVQGEHNRQNALAAIAAARSVGVP
HCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
PAQAARAFATFRNVKRRMEIRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGREHTRILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VLEPRSNTMKLGTMKAQLPASLADADLVFGYGAASGRDALGWSLAEALAPLGARAQAFDD
EECCCCCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
LHALVKAVAAAARPGDQILVMSNGGFGGVHQKLLDALSARGAAHGAEDRA
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]