Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 238026165

GI number: 238026165

Start: 522421

End: 523002

Strand: Direct

Name: ampD [H]

Synonym: bglu_1g04950

Alternate gene names: 238026165

Gene position: 522421-523002 (Clockwise)

Preceding gene: 238026164

Following gene: 238026166

Centisome position: 13.37

GC content: 69.07

Gene sequence:

>582_bases
ATGAGCGAGGTGCTCGAGGTCGGCGCCGATGGCTGGCTGCCTGGCGCCCGCCACGCGCCGTCGCCGAATCACGAGGCGCG
GCCGGCGGGCGTGGTGCCGACCCTGGTGGTAGTCCACAACATCAGCCTGCCGCCCGGCGAGTTCGGCGGCGAGGCGATCG
TCGCACTGTTCCAGAACCGGCTCGACTGCGACGCGCACCCCTATTACGACCTGAACCTGCGCGGGCTGCGCGTATCGGCG
CATTTCCTGATCGCGCGCGACGGTGAACTGATCCAGTTCGTGTCCTGCGAGGCGCGTGCCTGGCACGCCGGCATCTCGAC
CTTTCTCGGCCGCGAGCGCTGCAACGACTTCTCGATCGGCATCGAGCTCGAGGGCACCGACGACCTGCCGTTCGAGCCCG
CCCAATATGTGACACTCGCGGCACTCGTGCGCGCGCTCGCGGCCCGCTATCCGATCGAGGCGGTCGCCGGTCATGCGGAC
ATCGCGCCGGGCCGCAAGACCGACCCCGGTCCGCACTTCGATTGGCAACGGCTGGCAGCCGATGCCGGCCTGCCGCCCCG
ATACTTTCCTTATCGTCGGTAA

Upstream 100 bases:

>100_bases
GCGTGCACACGCCGAAGGGCGATGCGGTCGAGGCCGGCGGCGACTACTTCTGCAGTCGCGAGCACGCGGCCCGTCACGCG
GCGGCCGCGCGCGACGCGCA

Downstream 100 bases:

>100_bases
CAGGCGCGGCGTTCGAGCGCGTCGCGCCGACGCTGGGCGGCACGGGACGGCGCGATCCTTTGCCCCGTCTGCACTCGCCT
GATGTCAAGCCAAATCGCGC

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSEVLEVGADGWLPGARHAPSPNHEARPAGVVPTLVVVHNISLPPGEFGGEAIVALFQNRLDCDAHPYYDLNLRGLRVSA
HFLIARDGELIQFVSCEARAWHAGISTFLGRERCNDFSIGIELEGTDDLPFEPAQYVTLAALVRALAARYPIEAVAGHAD
IAPGRKTDPGPHFDWQRLAADAGLPPRYFPYRR

Sequences:

>Translated_193_residues
MSEVLEVGADGWLPGARHAPSPNHEARPAGVVPTLVVVHNISLPPGEFGGEAIVALFQNRLDCDAHPYYDLNLRGLRVSA
HFLIARDGELIQFVSCEARAWHAGISTFLGRERCNDFSIGIELEGTDDLPFEPAQYVTLAALVRALAARYPIEAVAGHAD
IAPGRKTDPGPHFDWQRLAADAGLPPRYFPYRR
>Mature_192_residues
SEVLEVGADGWLPGARHAPSPNHEARPAGVVPTLVVVHNISLPPGEFGGEAIVALFQNRLDCDAHPYYDLNLRGLRVSAH
FLIARDGELIQFVSCEARAWHAGISTFLGRERCNDFSIGIELEGTDDLPFEPAQYVTLAALVRALAARYPIEAVAGHADI
APGRKTDPGPHFDWQRLAADAGLPPRYFPYRR

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=170, Percent_Identity=61.1764705882353, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1787092, Length=126, Percent_Identity=46.031746031746, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21100; Mature: 20969

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVLEVGADGWLPGARHAPSPNHEARPAGVVPTLVVVHNISLPPGEFGGEAIVALFQNR
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHC
LDCDAHPYYDLNLRGLRVSAHFLIARDGELIQFVSCEARAWHAGISTFLGRERCNDFSIG
CCCCCCCCEECCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEE
IELEGTDDLPFEPAQYVTLAALVRALAARYPIEAVAGHADIAPGRKTDPGPHFDWQRLAA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
DAGLPPRYFPYRR
CCCCCCCCCCCCC
>Mature Secondary Structure 
SEVLEVGADGWLPGARHAPSPNHEARPAGVVPTLVVVHNISLPPGEFGGEAIVALFQNR
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHC
LDCDAHPYYDLNLRGLRVSAHFLIARDGELIQFVSCEARAWHAGISTFLGRERCNDFSIG
CCCCCCCCEECCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEE
IELEGTDDLPFEPAQYVTLAALVRALAARYPIEAVAGHADIAPGRKTDPGPHFDWQRLAA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
DAGLPPRYFPYRR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]