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The map label for this gene is proS [H]

Identifier: 238026159

GI number: 238026159

Start: 516555

End: 518291

Strand: Direct

Name: proS [H]

Synonym: bglu_1g04890

Alternate gene names: 238026159

Gene position: 516555-518291 (Clockwise)

Preceding gene: 238026156

Following gene: 238026160

Centisome position: 13.22

GC content: 66.84

Gene sequence:

>1737_bases
ATGAAAGCCTCCCGTTTCTTTATCGGCACCTTGAAGGAAGCACCCGCCGACGCCGAGATCGTCAGTCACAAACTGATGGT
GCGCGCCGGCATGATTCGCCGCATCGCCGGCGGCATCTACAACTATCTGCCGATCGGCCTGCGTTCGATCCGCAAGGTCG
AGGCGATCGTGCGCGAGGAGATGAACCGGGCTGGCGCGATCGAACTGCTGATGCCCGCGGTGCAGCCGGCCGAGCTCTGG
CAGGAGTCGGGGCGCTGGGAGCAGTACGGCCCGGAACTGCTGCGCTTCAAGGACCGCAAGGACAACGACTTCGTGATCGG
GCCGACGCACGAGGAAGTCGTCACCGACATCGCGCGCAACCAGATCAAGAGCTACCGGCAGATGCCGGTGAATTTCTACC
AGATCCAGACCAAGTTCCGCGACGAGATCCGGCCGCGCTTCGGCGTGATGCGCGGCCGTGAATTCATCATGAAGGACGCG
TACTCGTTCGACAAGGATCAGGCCGGCCTCAAGGAGTCGTATCGCAAGATGTACGACGCCTATGTGCGCGTGTTCACGCG
CATCGGCCTGGAGTTCCGCGCGGTCGCGGCCGACAACGGCTCGATCGGCGGCAGCGGCTCGCACGAATTCCACGTGATTG
CCGACACCGGCGAGGACGCGATCGCCTACTGTCCGACTTCCGACTTCGCCGCCAACGTCGAGGCCGCCGAGGCGCTGCCG
CTGTTGGCGAGCCGCGCGGCGCCGGCCGAGGCGATGGCGAAGGTGGCGACGCCGGGCAAGGCGAAGTGCGAGGCGGTGGC
CGAGCTGCTGAACATCCCGCTCGAGCGCACCATCAAGTCGATCGTCCTCGCCACCGAGAACGAGGGCGCCGAGCCGACCA
TCTGGCTGCTGATGCTGCGCGGCGACCACGACCTCAACGAGATCAAGGCCGCCAAGCAGCCCGGCCTGGCCGGCTACCGG
ATGGCCAGCGAGGCCGAGATCGTCGAATGGTTCGGCACGCCGCCGGGCTACCTGGGCCCGGTCGGCACGAAGAAGCCGGT
CAAGGTGATCGCCGACCGCACGGTGGCGAACATGAGCGATTTCGTGGTCGGCGCGAACGAGGTCGACTACCACATCGCTG
GCGTGAACTGGGGCCGGGATCTCCCGGAGCCGGTGGTTGCCGATCTGCGCAACGTGAAAAAGGGCGATCCGTCGCCGGAT
GGCAAGGGCGTGATCGACATCTGCCGCGGCATCGAGGTCGGCCATGTGTTCCAGCTCGGCACCAAGTATTCGAAGGCGCT
GGGCGCGACCTTCCTGGACGAGGGCGGCAAGCCCCAGCCGATGGAAATGGGCTGCTACGGCATCGGCGTGACGCGGATCC
TCGGCGCGGCGATCGAACAGAACTTCGACGACAGGGGGATCATCTGGCCCGAGGCGATCGCGCCGTTCGAGGTCGTGCTT
TGCCCGATGGGCTATGACCGCAGCGAGCTGGTGCGCGAGACGGCCGACCAGCTTTACGCGGCGCTGTCGGCGGCCGGCAT
CGATGTGATCCTCGACGATCGCGGCGAGCGTCCCGGCGTGATGTTCGCCGACTGGGAGTTGATCGGCGTGCCGCATCGTC
TCGTGATCGGCGAGCGCGGCCTGAAGGACGGCAAGATCGAGTACCAGGGCCGGCGCGACACGGAGGCCACGCTGCTGCCG
GCCGACGGCGCGGCTGCGACCGTGATCGAGACGGTCCGCGCGGCGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
ACGTCATCTGCGGTGCAGCTTCGCCTTGCACGCAGCTTTCCATTACTTTTCGCTTTTTTCTGGCGGCCCGGATTGGGCCG
CCGTTTCTGGAACTGTCCGC

Downstream 100 bases:

>100_bases
TGCCGTGAAGGCGGCGGGCGGGACGGCGCGATGCCGAGCGGAATCGCAACCGGGGCAGGGGGGCCGATAGCGGATGGAAT
ATAACTTCATGTCGGCCACC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDQAGLKESYRKMYDAYVRVFTRIGLEFRAVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMAKVATPGKAKCEAVAELLNIPLERTIKSIVLATENEGAEPTIWLLMLRGDHDLNEIKAAKQPGLAGYR
MASEAEIVEWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADLRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSKALGATFLDEGGKPQPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPEAIAPFEVVL
CPMGYDRSELVRETADQLYAALSAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
ADGAAATVIETVRAALAR

Sequences:

>Translated_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDQAGLKESYRKMYDAYVRVFTRIGLEFRAVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMAKVATPGKAKCEAVAELLNIPLERTIKSIVLATENEGAEPTIWLLMLRGDHDLNEIKAAKQPGLAGYR
MASEAEIVEWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADLRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSKALGATFLDEGGKPQPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPEAIAPFEVVL
CPMGYDRSELVRETADQLYAALSAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
ADGAAATVIETVRAALAR
>Mature_578_residues
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLPIGLRSIRKVEAIVREEMNRAGAIELLMPAVQPAELW
QESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARNQIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDA
YSFDKDQAGLKESYRKMYDAYVRVFTRIGLEFRAVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
LLASRAAPAEAMAKVATPGKAKCEAVAELLNIPLERTIKSIVLATENEGAEPTIWLLMLRGDHDLNEIKAAKQPGLAGYR
MASEAEIVEWFGTPPGYLGPVGTKKPVKVIADRTVANMSDFVVGANEVDYHIAGVNWGRDLPEPVVADLRNVKKGDPSPD
GKGVIDICRGIEVGHVFQLGTKYSKALGATFLDEGGKPQPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPEAIAPFEVVL
CPMGYDRSELVRETADQLYAALSAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERGLKDGKIEYQGRRDTEATLLP
ADGAAATVIETVRAALAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=43.2314410480349, Blast_Score=220, Evalue=3e-57,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=56.6202090592335, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=252, Percent_Identity=36.9047619047619, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=82, Percent_Identity=41.4634146341463, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=221, Percent_Identity=38.9140271493213, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=37.5510204081633, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63498; Mature: 63498

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDQAGLKESYRKMYDA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVRVFTRIGLEFRAVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHH
LLASRAAPAEAMAKVATPGKAKCEAVAELLNIPLERTIKSIVLATENEGAEPTIWLLMLR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKAAKQPGLAGYRMASEAEIVEWFGTPPGYLGPVGTKKPVKVIADRTVANMSD
CCCCHHHHHHHCCCCCCCEECCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPVVADLRNVKKGDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHH
TKYSKALGATFLDEGGKPQPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPEAIAPFEVVL
HHHHHHHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSELVRETADQLYAALSAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDTEATLLPADGAAATVIETVRAALAR
CCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASRFFIGTLKEAPADAEIVSHKLMVRAGMIRRIAGGIYNYLPIGLRSIRKVEAIVREE
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIELLMPAVQPAELWQESGRWEQYGPELLRFKDRKDNDFVIGPTHEEVVTDIARN
HHHCCCHHHHHCCCCHHHHHHHCCCHHHCCCHHHHCCCCCCCCEEECCCHHHHHHHHHHH
QIKSYRQMPVNFYQIQTKFRDEIRPRFGVMRGREFIMKDAYSFDKDQAGLKESYRKMYDA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YVRVFTRIGLEFRAVAADNGSIGGSGSHEFHVIADTGEDAIAYCPTSDFAANVEAAEALP
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHH
LLASRAAPAEAMAKVATPGKAKCEAVAELLNIPLERTIKSIVLATENEGAEPTIWLLMLR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCEEEEEEEE
GDHDLNEIKAAKQPGLAGYRMASEAEIVEWFGTPPGYLGPVGTKKPVKVIADRTVANMSD
CCCCHHHHHHHCCCCCCCEECCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHH
FVVGANEVDYHIAGVNWGRDLPEPVVADLRNVKKGDPSPDGKGVIDICRGIEVGHVFQLG
HEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHH
TKYSKALGATFLDEGGKPQPMEMGCYGIGVTRILGAAIEQNFDDRGIIWPEAIAPFEVVL
HHHHHHHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
CPMGYDRSELVRETADQLYAALSAAGIDVILDDRGERPGVMFADWELIGVPHRLVIGERG
ECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECEEEECCCCEEEECCCC
LKDGKIEYQGRRDTEATLLPADGAAATVIETVRAALAR
CCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA