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The map label for this gene is murD [H]

Identifier: 238026130

GI number: 238026130

Start: 485585

End: 487096

Strand: Direct

Name: murD [H]

Synonym: bglu_1g04600

Alternate gene names: 238026130

Gene position: 485585-487096 (Clockwise)

Preceding gene: 238026129

Following gene: 238026131

Centisome position: 12.43

GC content: 73.15

Gene sequence:

>1512_bases
ATGTTTGGAGATCGGCCGCGGCCGATGGTGCTCGTGCTGGGGCTCGGTGAATCGGGCCTCGCGATCGCGCGTTGGTGCGC
GAGGCACGCGTGCGCGCTGCGCGTCGCGGACACGCGCGAGGCGCCGCCCAATCTCGCCCTGCTGCGCGAGGCCGTGGCCG
ACGCTGAATTCGTCGGCGGCGCCTTTACGCCGGCGCTGCTCGACGGCGGCATCGAGATCGTTGCGCTGAGCCCGGGCCTG
TCGCCGCTCGAACCGGCGCTGGCGACGCTGCTCGCGGCCGCGCGCGAACGCGGCATCGCGGTCTGGGGCGAGCTCGAATT
CTTCGCGCGCGCGCTGGCCGGGCTCGCCGCGAACGGCTACGCGCCAAAAGTGCTTGCGATCACCGGCACCAACGGCAAGA
CCACCACCACCAGCCTGACCGGCCTGCTTTGCGAACGGGCGGGACGCTCGGTGGCGGTGGCCGGCAACATCAGCCCGGCG
ATGCTCGACCGGCTGTCGGCCGCGATCGACGCCGCCGCGCTGCCGCAGGTCTGGGTGCTCGAGCTGTCGAGCTTCCAGCT
CGAGACGGCCCAGAGCTTCGCGCCCGACGCGGCGGCGGTGCTCAATATCACCCAGGATCATCTCGATTGGCACGGCGGCT
TCGCTGCCTACGCGGCCGCGAAGGGCCGCATCTTCGGGCCGCGCACGGTGCGCGTGCTGAATCGCGACGACGCCGAGACG
ATGAAGTTCATGCCGCCCGCGGCGGCGGCCGACGCACCGCGCGCGATCACGTTCGGCCTGAACGAGCCGAGCCGCGAGGG
CGACTACGGCCTCGTGCGCGACAGCGGCATCAACTGGCTGGTGGAGGCGGTCGATCGCGACGCCGCCGAGGAGCCGGTTT
CGTCGCGGCGCCGCAAGGCCGGCGCGCCGGCCGCGCCCACGCTCACCGCCAAGCGTCTGATGCCGGCCGACGCGCTGCGC
ATCCGTGGGCTGCACAACGCGGCCAACGCGCTGGCCGCCTTCGCGCTGGCGCGCGCGATCGAGCTGCCGAGCGCCGCGCT
GCTGCACGGGCTGCGCGAATACCGCGGCGAGGCGCATCGCGTCGAGCTGATCGCCTCGCTCGACGGTGTCGATTACGTCG
ACGACAGCAAGGGCACCAACGTCGGCGCGACGGTCGCCGCGCTCGACGGCCTCGCGCAGCGCGCGGTGCTGATCGCCGGC
GGCGATGGCAAGGGCCAGGATTTCTCGCCGCTCGCGGCGCCCGTGGCGCGCTGGTGCCGCGCGGTGATGCTGATCGGCCG
CGACGCGCCGCGGATTCGCGCGGCGCTGGCCGACAGCGGCGTGCCGCTCGTCGAGCACGCCACGCTCGAGGCCGCCGTGC
GCGCCGCGAGCGAGCTGGCCGAACCCGGCGACGCGGTGCTGCTGTCGCCGGCCTGCGCGAGCTTCGACATGTTTAGCGAT
TATGCGCATCGCGCCGCGGTGTTCCGCAGTGCGCTCGAGGACATCGCACTCGACAGGGGGACGTCGCTATGA

Upstream 100 bases:

>100_bases
CAGGTCGTGGTGCGTTTCTGGATCATCACGCTGATGCTCTGCCTGTTCGGGTTGACCACGCTGAAACTGCGGTAAAGGGA
TAGCAAGGATGTCTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCTTCGTCTCGCGCTTCATCGGCCAGCGCGAAGGCGGCGGCGCCGTCGCGCGCCCGCCCGCCCGCACC
ACGCCGGCCGCGAACGGGTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MFGDRPRPMVLVLGLGESGLAIARWCARHACALRVADTREAPPNLALLREAVADAEFVGGAFTPALLDGGIEIVALSPGL
SPLEPALATLLAAARERGIAVWGELEFFARALAGLAANGYAPKVLAITGTNGKTTTTSLTGLLCERAGRSVAVAGNISPA
MLDRLSAAIDAAALPQVWVLELSSFQLETAQSFAPDAAAVLNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAET
MKFMPPAAAADAPRAITFGLNEPSREGDYGLVRDSGINWLVEAVDRDAAEEPVSSRRRKAGAPAAPTLTAKRLMPADALR
IRGLHNAANALAAFALARAIELPSAALLHGLREYRGEAHRVELIASLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAG
GDGKGQDFSPLAAPVARWCRAVMLIGRDAPRIRAALADSGVPLVEHATLEAAVRAASELAEPGDAVLLSPACASFDMFSD
YAHRAAVFRSALEDIALDRGTSL

Sequences:

>Translated_503_residues
MFGDRPRPMVLVLGLGESGLAIARWCARHACALRVADTREAPPNLALLREAVADAEFVGGAFTPALLDGGIEIVALSPGL
SPLEPALATLLAAARERGIAVWGELEFFARALAGLAANGYAPKVLAITGTNGKTTTTSLTGLLCERAGRSVAVAGNISPA
MLDRLSAAIDAAALPQVWVLELSSFQLETAQSFAPDAAAVLNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAET
MKFMPPAAAADAPRAITFGLNEPSREGDYGLVRDSGINWLVEAVDRDAAEEPVSSRRRKAGAPAAPTLTAKRLMPADALR
IRGLHNAANALAAFALARAIELPSAALLHGLREYRGEAHRVELIASLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAG
GDGKGQDFSPLAAPVARWCRAVMLIGRDAPRIRAALADSGVPLVEHATLEAAVRAASELAEPGDAVLLSPACASFDMFSD
YAHRAAVFRSALEDIALDRGTSL
>Mature_503_residues
MFGDRPRPMVLVLGLGESGLAIARWCARHACALRVADTREAPPNLALLREAVADAEFVGGAFTPALLDGGIEIVALSPGL
SPLEPALATLLAAARERGIAVWGELEFFARALAGLAANGYAPKVLAITGTNGKTTTTSLTGLLCERAGRSVAVAGNISPA
MLDRLSAAIDAAALPQVWVLELSSFQLETAQSFAPDAAAVLNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAET
MKFMPPAAAADAPRAITFGLNEPSREGDYGLVRDSGINWLVEAVDRDAAEEPVSSRRRKAGAPAAPTLTAKRLMPADALR
IRGLHNAANALAAFALARAIELPSAALLHGLREYRGEAHRVELIASLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAG
GDGKGQDFSPLAAPVARWCRAVMLIGRDAPRIRAALADSGVPLVEHATLEAAVRAASELAEPGDAVLLSPACASFDMFSD
YAHRAAVFRSALEDIALDRGTSL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=483, Percent_Identity=38.0952380952381, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52487; Mature: 52487

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRPRPMVLVLGLGESGLAIARWCARHACALRVADTREAPPNLALLREAVADAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCC
AFTPALLDGGIEIVALSPGLSPLEPALATLLAAARERGIAVWGELEFFARALAGLAANGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCC
APKVLAITGTNGKTTTTSLTGLLCERAGRSVAVAGNISPAMLDRLSAAIDAAALPQVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
ELSSFQLETAQSFAPDAAAVLNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAET
ECCCCHHHHHHHCCCCHHHHEECHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MKFMPPAAAADAPRAITFGLNEPSREGDYGLVRDSGINWLVEAVDRDAAEEPVSSRRRKA
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHCCHHCCHHHHHHHHC
GAPAAPTLTAKRLMPADALRIRGLHNAANALAAFALARAIELPSAALLHGLREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHE
VELIASLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFSPLAAPVARWCR
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHH
AVMLIGRDAPRIRAALADSGVPLVEHATLEAAVRAASELAEPGDAVLLSPACASFDMFSD
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
YAHRAAVFRSALEDIALDRGTSL
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGDRPRPMVLVLGLGESGLAIARWCARHACALRVADTREAPPNLALLREAVADAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCC
AFTPALLDGGIEIVALSPGLSPLEPALATLLAAARERGIAVWGELEFFARALAGLAANGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCC
APKVLAITGTNGKTTTTSLTGLLCERAGRSVAVAGNISPAMLDRLSAAIDAAALPQVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
ELSSFQLETAQSFAPDAAAVLNITQDHLDWHGGFAAYAAAKGRIFGPRTVRVLNRDDAET
ECCCCHHHHHHHCCCCHHHHEECHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCCCHHH
MKFMPPAAAADAPRAITFGLNEPSREGDYGLVRDSGINWLVEAVDRDAAEEPVSSRRRKA
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHCCHHCCHHHHHHHHC
GAPAAPTLTAKRLMPADALRIRGLHNAANALAAFALARAIELPSAALLHGLREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHE
VELIASLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFSPLAAPVARWCR
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHH
AVMLIGRDAPRIRAALADSGVPLVEHATLEAAVRAASELAEPGDAVLLSPACASFDMFSD
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
YAHRAAVFRSALEDIALDRGTSL
HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA