Click here to switch to the map view.

The map label for this gene is mlaE [H]

Identifier: 238025995

GI number: 238025995

Start: 342944

End: 343711

Strand: Direct

Name: mlaE [H]

Synonym: bglu_1g03210

Alternate gene names: 238025995

Gene position: 342944-343711 (Clockwise)

Preceding gene: 238025994

Following gene: 238025996

Centisome position: 8.78

GC content: 67.06

Gene sequence:

>768_bases
ATGATCAGCGCTATCGGTCGTTTCGTGCTGGGCGGGCTCGAACGCACGGGTTATGCCACGCGCTTGTTCGTGCGGCTCGT
GCTCGAATTCTTTCCGCTGCTGCGGCGCCCGCGGCTCGTCACGAAACAGATCCACTTCCTCGGCAATTATTCGTTTGTGA
TCATTGCCGTGTCGGGCCTGTTCGTCGGCTTCGTGCTGGGGCTGCAGGGCTACTACACGCTGAACCGCTACGGCGCCGAG
CAGTCGCTCGGGCTGCTGGTGGCGCTGTCGCTGGTGCGCGAACTCGGGCCGGTGGTCACCGCGCTGCTGTACGCGGGCCG
CGCCGGCACCTCGCTCACCGCCGAGATCGGCCTGATGAAGGCGGGCGAGCAGCTGACCGCGCTCGAGATGATGGCGGTGG
ACCCGATCAAGACCGTGATCGCGCCGCGCATGTGGGCCGGCATCATCGCGATGCCGCTGCTCTCGGCGATCTTCTGCGCG
GTGGGCGTGATCGGCGGTTACGTGGTGGGCGTGATCCTGATCGGCGTCGATCCGGGCGCGTTCTGGTCGCAGATGCAGGG
CGGCGTGGACGCCTGGAGCGACGTCGGCAACGGCGTGCTCAAGAGCGTCGTATTCGGCTTCGCGGTCACCTTCGTGGCGC
TCTACCAGGGCTACGAGGCGAAGCCGACGCCCGAGGGCGTCTCGCGCGCCACCACCCGCACCGTCGTGTACGCCTCGCTC
GCGGTACTCGGCCTCGATTTCCTGCTGACCGCGCTGATGTTCAGCTGA

Upstream 100 bases:

>100_bases
GCGTGCGGCAGTTCATCGACGGCGTGCCCGACGGCCCGTTCCGGTTCCACTACACGAGTCCGCCGCTCGATGCGGACTTC
GGCCTTGGCGGAGGGCGCCG

Downstream 100 bases:

>100_bases
GTCGGCCTTTTCAGAGATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTGTTCGTGCTGGTGGGTTTCCTGG
CGCTGCTGTTTCTCGCCCTG

Product: ABC transporter inner membrane subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRFVLGGLERTGYATRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGAE
QSLGLLVALSLVRELGPVVTALLYAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIIAMPLLSAIFCA
VGVIGGYVVGVILIGVDPGAFWSQMQGGVDAWSDVGNGVLKSVVFGFAVTFVALYQGYEAKPTPEGVSRATTRTVVYASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRFVLGGLERTGYATRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGAE
QSLGLLVALSLVRELGPVVTALLYAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIIAMPLLSAIFCA
VGVIGGYVVGVILIGVDPGAFWSQMQGGVDAWSDVGNGVLKSVVFGFAVTFVALYQGYEAKPTPEGVSRATTRTVVYASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRFVLGGLERTGYATRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGAE
QSLGLLVALSLVRELGPVVTALLYAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKTVIAPRMWAGIIAMPLLSAIFCA
VGVIGGYVVGVILIGVDPGAFWSQMQGGVDAWSDVGNGVLKSVVFGFAVTFVALYQGYEAKPTPEGVSRATTRTVVYASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=61.8181818181818, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27225; Mature: 27225

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure
MISAIGRFVLGGLERTGYATRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGL
CCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHH
FVGFVLGLQGYYTLNRYGAEQSLGLLVALSLVRELGPVVTALLYAGRAGTSLTAEIGLMK
HHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AGEQLTALEMMAVDPIKTVIAPRMWAGIIAMPLLSAIFCAVGVIGGYVVGVILIGVDPGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
FWSQMQGGVDAWSDVGNGVLKSVVFGFAVTFVALYQGYEAKPTPEGVSRATTRTVVYASL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AVLGLDFLLTALMFS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]