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The map label for this gene is gltB [H]

Identifier: 238025988

GI number: 238025988

Start: 334451

End: 335917

Strand: Direct

Name: gltB [H]

Synonym: bglu_1g03140

Alternate gene names: 238025988

Gene position: 334451-335917 (Clockwise)

Preceding gene: 238025987

Following gene: 238025990

Centisome position: 8.56

GC content: 67.62

Gene sequence:

>1467_bases
ATGGGCAAGGCAACCGGTTTTCTCGAGTTCGAACGCCGCCACGAGGCGTACGAGGCTCCGCTCACGCGCGTGAAGCACTA
CAAGGAATTCGTCGCGGCGCTGTCCGACGACGACGCGAAGATCCAGGGCGCGCGCTGCATGGATTGCGGCATCCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATCCCGGACTTCAACGACCTGGTGTACCGCCAGGACTGGCAGCAGGCCATC
GAGGTCCTCCACTCGACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAGGCGGCCTGCACGCT
CGGCATCAACAACGATCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCCTGGGCCGAAGGCTGGGTCA
AGCCGCTGCCGGCCGAACACAAGACCGGCAAGAAGGTCGCCGTGGTCGGCTCCGGCCCGGCGGGCCTGGCGGCCGCGCAG
CAGCTCGCGCGCGCCGGCCACGAGGTGACGGTGTTCGAGAAGAACGACCGGATCGGCGGCCTGCTGCGCTACGGCATCCC
CGACTTCAAGCTCGAGAAGTGGCTGATCGACCGCCGCATGCGCCAGATGGAAGCCGAGGGCGTCACGTTCCGCACCAGCG
TGTTCATCGGCAAGGAAGCGCTGCCGGACATGATCGGCAACATGGCGAAGGAAACCATCTCGCCCGAGCAGCTGAAGGAA
ACGTTCGACGCGGTGGTGATCGCAGGCGGCTCCGAGACGCCGCGCGACCTGCCGGTGCCGGGCCGCGAGCTCGAAGGCGT
CCATTTCGCGATGGATTTCCTGCCGCAGCAGAACCGCGTGAACGCCGGTGACAAGCTGGCCGACCAACTGCTTGCCAAGG
GCAAGCACGTCGTCGTGATCGGCGGCGGCGACACGGGTTCGGACTGCGTGGGCACCTCGAACCGCCACGGCGCGAAGTCG
GTCACGCAGTTCGAGCTGTTGCCGCAGCCGCCGGAAACCGAGAACAAGCCGCTCGTCTGGCCGTACTGGCCGATCAAGCT
GCGCACCTCGTCGTCGCACGAGGAAGGCTGCGAGCGCGACTGGGCGGTGGCGACCAAGCGCCTCGAGGGCAAGAACGGCA
AGGTCGAGAAGCTGATCGCCGTGCGCGTCGAATGGAAGGACGGCAAGATGGTCGAGGTGGCCGGCTCCGAGTTCGAGATG
AAGGCGGACCTGGTGCTGCTGGCGATGGGCTTCACGCAGCCGGCCGCGCCGGTGCTCGACGCGTTCGGCGTGGCCAAGGA
CGCGCGCGGCAACGCACGCGCCGCCACCGAAGGCGAGCGTGCCTACACGACCTCGGTCGACAAGGTGTTCGCGGCCGGCG
ACATGCGCCGCGGCCAGTCGCTGGTGGTCTGGGCGATCCGCGAAGGCCGCCAGTGCGCGCGCGCCGTCGACGCGTTCCTG
ATGGGGCATTCGGAACTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGGCGCACGGCAGCCGCGAAACGACATAGCGGACGCGCCCGCCGCCACCGGCGGGCGCGTTCCCCACCCGACACACCC
CCGATTCAGACGAGAACCTT

Downstream 100 bases:

>100_bases
GCGGGCGGCGGCGCGCGCCGCCGGACGAACGAAAAAAGAGCACGCCCCGGCGTGCTCTTTTTGCTGGCGGGGCCGGTCAG
CCCCGGTGTCTCAGGCCGTG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDDDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQ
QLARAGHEVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKEALPDMIGNMAKETISPEQLKE
TFDAVVIAGGSETPRDLPVPGRELEGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKS
VTQFELLPQPPETENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMVEVAGSEFEM
KADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGERAYTTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDAFL
MGHSELPR

Sequences:

>Translated_488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDDDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQ
QLARAGHEVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKEALPDMIGNMAKETISPEQLKE
TFDAVVIAGGSETPRDLPVPGRELEGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKS
VTQFELLPQPPETENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMVEVAGSEFEM
KADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGERAYTTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDAFL
MGHSELPR
>Mature_487_residues
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDDDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQ
LARAGHEVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKEALPDMIGNMAKETISPEQLKET
FDAVVIAGGSETPRDLPVPGRELEGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKSV
TQFELLPQPPETENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMVEVAGSEFEMK
ADLVLLAMGFTQPAAPVLDAFGVAKDARGNARAATEGERAYTTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDAFLM
GHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=491, Percent_Identity=24.6435845213849, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.9794238683128, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=35.7601713062098, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=34.4017094017094, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788468, Length=462, Percent_Identity=28.3549783549784, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=28.5393258426966, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=508, Percent_Identity=46.259842519685, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984108, Length=469, Percent_Identity=25.7995735607676, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=49.203187250996, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=49.5029821073559, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=49.5029821073559, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=49.5029821073559, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=49.5029821073559, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=484, Percent_Identity=23.3471074380165, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18858217, Length=484, Percent_Identity=23.3471074380165, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53503; Mature: 53371

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDDDAKIQGARCMDCGIPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQLARAGHEVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKEALPDMIGNMAKETISPEQLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHH
TFDAVVIAGGSETPRDLPVPGRELEGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVVVI
HHCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKSVTQFELLPQPPETENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMVEVAGSEFEMKADLVLLAMGFTQPAAPVLD
HHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEECCEEEEEECCCCCCHHHHH
AFGVAKDARGNARAATEGERAYTTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDAFL
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHH
MGHSELPR
HCCCCCCC
>Mature Secondary Structure 
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDDDAKIQGARCMDCGIPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPAEHKTGKKVAVVGSGPAGLAAAQQLARAGHEVTVFEKNDRIGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKEALPDMIGNMAKETISPEQLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHCCCHHHHHH
TFDAVVIAGGSETPRDLPVPGRELEGVHFAMDFLPQQNRVNAGDKLADQLLAKGKHVVVI
HHCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKSVTQFELLPQPPETENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMVEVAGSEFEMKADLVLLAMGFTQPAAPVLD
HHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEECCEEEEEECCCCCCHHHHH
AFGVAKDARGNARAATEGERAYTTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDAFL
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHH
MGHSELPR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]