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The map label for this gene is ngoPIIM [H]

Identifier: 238025826

GI number: 238025826

Start: 154861

End: 156084

Strand: Reverse

Name: ngoPIIM [H]

Synonym: bglu_1g01510

Alternate gene names: 238025826

Gene position: 156084-154861 (Counterclockwise)

Preceding gene: 238025827

Following gene: 238025825

Centisome position: 4.0

GC content: 61.19

Gene sequence:

>1224_bases
ATGAAGGCAGTTGAGCTATACGCAGGTGCGGGCGGCCTAGCGATGGGAGTCGGCCTTGCCGGCTTCAAACCTCTGGCAGT
GGTCGAATGGGACCGATGGGCGTGTGACACGATTCGTGAGAACCAGCGGCGAGGCTTTCCCTTGGTGGCAGATTGGCCTC
TTTACGAGGGTGACGTTCGCAAATTTGATTGGTCACGCCTCGAAGGCGAGGAAATCGACCTGTTAGCCGGCGGCCCCCCG
TGCCAACCGTTCTCGATGGGTGGCAAGCATCAGGCACACGACGACAGCCGTGACATGTTCCCTGCAACGGTGGATATCGT
GCGCAAACTGCGCCCGAAAGCCTTCATCGTGGAAAACGTCAAGGGCCTGACGCGCTCCACTTTTGCCAACTACTACCAAT
ACATCCTGCTGCAGCTCGAGTTTCCCGAAGTCCCGGCACGCCGCAACGAGGAATGGTTCGAGCATCACCTGCGCCTACAA
GCCGAACGCACGAGCGGCCGCCAGAAGGGCAAAAAGCTAACCTACAACGTGGTTCCCACGCTGGTAAATGCTGCCGACTA
TGGCGTTCCGCAAAAGCGTGAACGTGTATTCATCGTGGGCTTCCGCTCCGATCTTGACGTGGATTGGAGCTTTCCACGCC
CGACGCACAGCTACGACAGCCTGCTCCGTGACCAATGGGTCACTGGCGAATATTGGGAACGCCATCAAGTGCCGCGCCGT
GCGCGACCGACGATGCCGGAATCAGTGGCAAGCCGCGTGCGGCGCCTCGCTGGCTCGCTACCGTTCGCGACCGAGTTGCC
TTGGCGAACCGTCCGTGACGCGATTGCAGGCCTACCGAGCCCGCTTGCGCGGACGGCGAGTCAATACCAGGACCATCGCT
TCCAGCCGGGTGCCCGCGTCTATCCCGGCCACACTGGTAGCCCGCTCGATTTGCCGGCTAAAACTCTGAAAGCTGGCGAC
CATGGCGTGCCAGGCGGCGAGAACATGCTTGTCAACACGGACGGATCAGTCCGCTACTTCTCGATTCGCGAATCGGCGCG
TGTGCAAACCTTCCCCGACGGCTTCAAGTTCCACGGTTCATGGACAGAAACCATGCGCCAGCTCGGCAACGCCGTCCCTG
TCGCACTTGGCCACGTTGTGGCACGAAGCGTCGCGGAGCGTTTGGCGATCGTTCAACTTGAGCAACTGGCGCGCCGGCGC
GTAACGGTGCGAGGGGCGGCATGA

Upstream 100 bases:

>100_bases
CTCGGCTGGAAAGTTTTGACAATATGGGAATGTGAAGTGAAGGATGAGGTGGTCCTGACCCTCCGCGTCAGGGCCTTCTT
GGACGACACGGAGAACAACA

Downstream 100 bases:

>100_bases
CGGATTCGATTGGAAACATCATTCCCTTTAATCCGCTCGACAAGAAAAACCTCGGCGCAAGTGTCGCTGAGGCGATGCTC
GAACAGAAACCACAGCCTCT

Product: DNA cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoPII; Cytosine-specific methyltransferase NgoPII [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKAVELYAGAGGLAMGVGLAGFKPLAVVEWDRWACDTIRENQRRGFPLVADWPLYEGDVRKFDWSRLEGEEIDLLAGGPP
CQPFSMGGKHQAHDDSRDMFPATVDIVRKLRPKAFIVENVKGLTRSTFANYYQYILLQLEFPEVPARRNEEWFEHHLRLQ
AERTSGRQKGKKLTYNVVPTLVNAADYGVPQKRERVFIVGFRSDLDVDWSFPRPTHSYDSLLRDQWVTGEYWERHQVPRR
ARPTMPESVASRVRRLAGSLPFATELPWRTVRDAIAGLPSPLARTASQYQDHRFQPGARVYPGHTGSPLDLPAKTLKAGD
HGVPGGENMLVNTDGSVRYFSIRESARVQTFPDGFKFHGSWTETMRQLGNAVPVALGHVVARSVAERLAIVQLEQLARRR
VTVRGAA

Sequences:

>Translated_407_residues
MKAVELYAGAGGLAMGVGLAGFKPLAVVEWDRWACDTIRENQRRGFPLVADWPLYEGDVRKFDWSRLEGEEIDLLAGGPP
CQPFSMGGKHQAHDDSRDMFPATVDIVRKLRPKAFIVENVKGLTRSTFANYYQYILLQLEFPEVPARRNEEWFEHHLRLQ
AERTSGRQKGKKLTYNVVPTLVNAADYGVPQKRERVFIVGFRSDLDVDWSFPRPTHSYDSLLRDQWVTGEYWERHQVPRR
ARPTMPESVASRVRRLAGSLPFATELPWRTVRDAIAGLPSPLARTASQYQDHRFQPGARVYPGHTGSPLDLPAKTLKAGD
HGVPGGENMLVNTDGSVRYFSIRESARVQTFPDGFKFHGSWTETMRQLGNAVPVALGHVVARSVAERLAIVQLEQLARRR
VTVRGAA
>Mature_407_residues
MKAVELYAGAGGLAMGVGLAGFKPLAVVEWDRWACDTIRENQRRGFPLVADWPLYEGDVRKFDWSRLEGEEIDLLAGGPP
CQPFSMGGKHQAHDDSRDMFPATVDIVRKLRPKAFIVENVKGLTRSTFANYYQYILLQLEFPEVPARRNEEWFEHHLRLQ
AERTSGRQKGKKLTYNVVPTLVNAADYGVPQKRERVFIVGFRSDLDVDWSFPRPTHSYDSLLRDQWVTGEYWERHQVPRR
ARPTMPESVASRVRRLAGSLPFATELPWRTVRDAIAGLPSPLARTASQYQDHRFQPGARVYPGHTGSPLDLPAKTLKAGD
HGVPGGENMLVNTDGSVRYFSIRESARVQTFPDGFKFHGSWTETMRQLGNAVPVALGHVVARSVAERLAIVQLEQLARRR
VTVRGAA

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 45912; Mature: 45912

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVELYAGAGGLAMGVGLAGFKPLAVVEWDRWACDTIRENQRRGFPLVADWPLYEGDVR
CCCEEEECCCCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCC
KFDWSRLEGEEIDLLAGGPPCQPFSMGGKHQAHDDSRDMFPATVDIVRKLRPKAFIVENV
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHH
KGLTRSTFANYYQYILLQLEFPEVPARRNEEWFEHHLRLQAERTSGRQKGKKLTYNVVPT
HHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCEEEEEHHHH
LVNAADYGVPQKRERVFIVGFRSDLDVDWSFPRPTHSYDSLLRDQWVTGEYWERHQVPRR
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCC
ARPTMPESVASRVRRLAGSLPFATELPWRTVRDAIAGLPSPLARTASQYQDHRFQPGARV
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
YPGHTGSPLDLPAKTLKAGDHGVPGGENMLVNTDGSVRYFSIRESARVQTFPDGFKFHGS
CCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCEECCC
WTETMRQLGNAVPVALGHVVARSVAERLAIVQLEQLARRRVTVRGAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCC
>Mature Secondary Structure
MKAVELYAGAGGLAMGVGLAGFKPLAVVEWDRWACDTIRENQRRGFPLVADWPLYEGDVR
CCCEEEECCCCCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCC
KFDWSRLEGEEIDLLAGGPPCQPFSMGGKHQAHDDSRDMFPATVDIVRKLRPKAFIVENV
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHH
KGLTRSTFANYYQYILLQLEFPEVPARRNEEWFEHHLRLQAERTSGRQKGKKLTYNVVPT
HHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHCCCEEEEEHHHH
LVNAADYGVPQKRERVFIVGFRSDLDVDWSFPRPTHSYDSLLRDQWVTGEYWERHQVPRR
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCC
ARPTMPESVASRVRRLAGSLPFATELPWRTVRDAIAGLPSPLARTASQYQDHRFQPGARV
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
YPGHTGSPLDLPAKTLKAGDHGVPGGENMLVNTDGSVRYFSIRESARVQTFPDGFKFHGS
CCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCEECCC
WTETMRQLGNAVPVALGHVVARSVAERLAIVQLEQLARRRVTVRGAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837733; 2501649; 8406039 [H]