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The map label for this gene is dfrA [H]

Identifier: 238025518

GI number: 238025518

Start: 2746986

End: 2747996

Strand: Direct

Name: dfrA [H]

Synonym: bglu_2g22140

Alternate gene names: 238025518

Gene position: 2746986-2747996 (Clockwise)

Preceding gene: 238025514

Following gene: 238025519

Centisome position: 97.16

GC content: 70.43

Gene sequence:

>1011_bases
ATGACAGACACACAACGCGATCTCGTACTCGTCACCGGCGCGTCCGGCTTCGTCGGCTCCGCCGTGGCACGCATCGCGCA
GGCGCACGGTTATGCGCTGCGCGTGCTGGTGCGCGCCACGAGCCCGCGCACCAACCTCGCCGGCCTCGACGCCGAGGTCG
TCACGGGCGACATGCGCGACGAGGCGTCGATGCGCCAGGCACTGCGCGGGGTGCGCCATCTGCTGCACGTGGCCGCCGAC
TACCGGCTCTGGGCACCCGATCCGCACGAAATCGAGCGCGCCAACCTGGAAGGCGCCGTGGCCACGATGCGCGCGGCGCT
CGCCGAGGGCGTCGAGCGCATCGTCTACACCAGCAGCGTCGCCACGCTGAAGGTGACGAATTCCGGCAACTCCACCGACG
AGACCTCGCCGCTCGCGGCCGAGCAGGCGATCGGCGTCTACAAGCGCAGCAAGGTGCTGGCCGAGCGCGCGGTGGAGCGC
ATGATCGCCGAGGACGGCCTGCCCGCCGTGATCGTCAACCCGTCCACGCCGATCGGCCCGCGCGACGTGAAGCCGACGCC
CACCGGACGCATCATCGTCGAGGCCGCCACCGGCAAGATTCCGGCGTTCGTCGATACGGGCCTGAACCTGGTCCACGTCG
ACGACGTCGCGCACGGCCATTTCCTCGCGCTCGAGAAAGGCCGCATCGGCGAGCGCTACATCCTCGGCGGCGAGAACCTG
CCGCTGCAGCAGATGCTGGCCGACATCGCCGCGATGGTCGGCCGCAAACCGCCCACCGTCGCGCTGCCGCGCTGGCCGCT
CTACCCGCTCGCGGTCGCGGCCGAGGCGGTGGCGAAGTTCACGAAGCGCGAGCCGTTCGTGACCGTCGACGGGCTGAAGA
TGTCGAAGAACAAGATGTACTTCACGTCGGCGAAGGCCGAGCGCGAGCTCGGCTACCGCGCGCGGCCCTACCGCGACGGC
CTGCGCGATGCGCTCGACTGGTTCCGCGAGGCCGGCTATCTGACGCGCTGA

Upstream 100 bases:

>100_bases
CAGCGCGCGCGGGCGCCGCGGCCGCGGCCGGCTGCACGTACAATCGCGCATCACGCACAACCGCCGCGCGCCCGAGCCGC
GCGCGGCCCCGAGGAACGGC

Downstream 100 bases:

>100_bases
ACGCGGCGGCGGGCGCGGGGCGGCCCCGCCCGAGCCGCTGCACGGCCCACGGATGCGAGCGCCCGCTTCGGGCGACCGGG
TCCGCGCGCGGCGGCGCTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTDTQRDLVLVTGASGFVGSAVARIAQAHGYALRVLVRATSPRTNLAGLDAEVVTGDMRDEASMRQALRGVRHLLHVAAD
YRLWAPDPHEIERANLEGAVATMRAALAEGVERIVYTSSVATLKVTNSGNSTDETSPLAAEQAIGVYKRSKVLAERAVER
MIAEDGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAHGHFLALEKGRIGERYILGGENL
PLQQMLADIAAMVGRKPPTVALPRWPLYPLAVAAEAVAKFTKREPFVTVDGLKMSKNKMYFTSAKAERELGYRARPYRDG
LRDALDWFREAGYLTR

Sequences:

>Translated_336_residues
MTDTQRDLVLVTGASGFVGSAVARIAQAHGYALRVLVRATSPRTNLAGLDAEVVTGDMRDEASMRQALRGVRHLLHVAAD
YRLWAPDPHEIERANLEGAVATMRAALAEGVERIVYTSSVATLKVTNSGNSTDETSPLAAEQAIGVYKRSKVLAERAVER
MIAEDGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAHGHFLALEKGRIGERYILGGENL
PLQQMLADIAAMVGRKPPTVALPRWPLYPLAVAAEAVAKFTKREPFVTVDGLKMSKNKMYFTSAKAERELGYRARPYRDG
LRDALDWFREAGYLTR
>Mature_335_residues
TDTQRDLVLVTGASGFVGSAVARIAQAHGYALRVLVRATSPRTNLAGLDAEVVTGDMRDEASMRQALRGVRHLLHVAADY
RLWAPDPHEIERANLEGAVATMRAALAEGVERIVYTSSVATLKVTNSGNSTDETSPLAAEQAIGVYKRSKVLAERAVERM
IAEDGLPAVIVNPSTPIGPRDVKPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAHGHFLALEKGRIGERYILGGENLP
LQQMLADIAAMVGRKPPTVALPRWPLYPLAVAAEAVAKFTKREPFVTVDGLKMSKNKMYFTSAKAERELGYRARPYRDGL
RDALDWFREAGYLTR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=358, Percent_Identity=25.6983240223464, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI193211614, Length=331, Percent_Identity=24.773413897281, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI8393516, Length=331, Percent_Identity=24.773413897281, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI310132178, Length=361, Percent_Identity=24.3767313019391, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI310113012, Length=361, Percent_Identity=24.3767313019391, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71987463, Length=351, Percent_Identity=28.4900284900285, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 36486; Mature: 36355

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQRDLVLVTGASGFVGSAVARIAQAHGYALRVLVRATSPRTNLAGLDAEVVTGDMRD
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHEEECCCHH
EASMRQALRGVRHLLHVAADYRLWAPDPHEIERANLEGAVATMRAALAEGVERIVYTSSV
HHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCE
ATLKVTNSGNSTDETSPLAAEQAIGVYKRSKVLAERAVERMIAEDGLPAVIVNPSTPIGP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RDVKPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAHGHFLALEKGRIGERYILGGENL
CCCCCCCCCEEEEEECCCCCCCHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCC
PLQQMLADIAAMVGRKPPTVALPRWPLYPLAVAAEAVAKFTKREPFVTVDGLKMSKNKMY
CHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEE
FTSAKAERELGYRARPYRDGLRDALDWFREAGYLTR
EECCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDTQRDLVLVTGASGFVGSAVARIAQAHGYALRVLVRATSPRTNLAGLDAEVVTGDMRD
CCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHEEECCCHH
EASMRQALRGVRHLLHVAADYRLWAPDPHEIERANLEGAVATMRAALAEGVERIVYTSSV
HHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCE
ATLKVTNSGNSTDETSPLAAEQAIGVYKRSKVLAERAVERMIAEDGLPAVIVNPSTPIGP
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
RDVKPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVAHGHFLALEKGRIGERYILGGENL
CCCCCCCCCEEEEEECCCCCCCHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCC
PLQQMLADIAAMVGRKPPTVALPRWPLYPLAVAAEAVAKFTKREPFVTVDGLKMSKNKMY
CHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCCEEE
FTSAKAERELGYRARPYRDGLRDALDWFREAGYLTR
EECCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]