Click here to switch to the map view.

The map label for this gene is argT [H]

Identifier: 238025448

GI number: 238025448

Start: 2657901

End: 2658683

Strand: Direct

Name: argT [H]

Synonym: bglu_2g21400

Alternate gene names: 238025448

Gene position: 2657901-2658683 (Clockwise)

Preceding gene: 238025445

Following gene: 238025449

Centisome position: 94.01

GC content: 65.39

Gene sequence:

>783_bases
GTGAAAAAAGTTCTGGCGGCCCTGACGGTTGCCCTGCTTGCCGTGTCGGCAGGCGGCGCCTACGCAAAAGACTGGTCGAC
GGTGCGCTTCGGCGTCGACGCGAGCTACCCGCCGTTCGAATCGAAGGGCGCGGACGGCAAGCTGGTCGGCTTCGACATCG
ACCTGGGCAATGAAATCTGCCGCCGCATCGCCGCGAAGTGCGTCTGGGTCGAGAACGACTTCGACGGGTTGATCCCGGCG
CTGAAGGCCCGCAAGTTCGACGGCGTGCTGTCGTCGATGTCGATGACGCCGCAACGCGCCGAACAGATCGCCTTCTCGGA
CAAGCTGTTCAACACGCCGACGCGCCTCGTCGCCAAGAAGGGCTCGAACATCCTGCCGACGGCCGCCTCGCTGAAGGGCA
AGACGGTGGGCGTGGAGCAGGGCACGATCCAGGAAACCTACGCGAAGACCTACTGGGCACCGAAGGGCGTGAACGTCCAG
CCGTACCAGAACCAGGACCAGGTCTACGCCGACCTGCTCTCGGGCCGCCTCGACGCCACGCTGCAGGACGCGGTGCAGGC
CGAGATCGGCTTCCTGAAGACGCCGCGCGGCGCCGGCTACGCGTTCGCCGGCGGCGACATCGACGATCCGAAGACGCTCG
GCGAAGGCGCCGGCGTGGGCCTGCGCAAGGAAGACACCGACCTGAAGGCGAAGATCGACGGCGCGATCGTCGCGATGCGC
AAGGACGGCACCTACGACAAGATCGCCAAGAAGTACTTCGATTTCGACGTCTACGGCAAGTAA

Upstream 100 bases:

>100_bases
CCATATCGGGCGGGGGTCCGAGTCAGCCCGGCCGCTGCGGCGCCGGGCGAAGCGCACTGCGGCACGACCGCGCGCAAACC
ACGTACAACGAGGAGTCATA

Downstream 100 bases:

>100_bases
GCACCGCCTCGACGCGTGCGCCGACGGGCCCTGCGGGGCCCGTTGTCATTGGCGCGGGCGCCGCGCCCCCGCCGCCGTGC
CGCGCGCCGCGACCGGCCCC

Product: Cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKVLAALTVALLAVSAGGAYAKDWSTVRFGVDASYPPFESKGADGKLVGFDIDLGNEICRRIAAKCVWVENDFDGLIPA
LKARKFDGVLSSMSMTPQRAEQIAFSDKLFNTPTRLVAKKGSNILPTAASLKGKTVGVEQGTIQETYAKTYWAPKGVNVQ
PYQNQDQVYADLLSGRLDATLQDAVQAEIGFLKTPRGAGYAFAGGDIDDPKTLGEGAGVGLRKEDTDLKAKIDGAIVAMR
KDGTYDKIAKKYFDFDVYGK

Sequences:

>Translated_260_residues
MKKVLAALTVALLAVSAGGAYAKDWSTVRFGVDASYPPFESKGADGKLVGFDIDLGNEICRRIAAKCVWVENDFDGLIPA
LKARKFDGVLSSMSMTPQRAEQIAFSDKLFNTPTRLVAKKGSNILPTAASLKGKTVGVEQGTIQETYAKTYWAPKGVNVQ
PYQNQDQVYADLLSGRLDATLQDAVQAEIGFLKTPRGAGYAFAGGDIDDPKTLGEGAGVGLRKEDTDLKAKIDGAIVAMR
KDGTYDKIAKKYFDFDVYGK
>Mature_260_residues
MKKVLAALTVALLAVSAGGAYAKDWSTVRFGVDASYPPFESKGADGKLVGFDIDLGNEICRRIAAKCVWVENDFDGLIPA
LKARKFDGVLSSMSMTPQRAEQIAFSDKLFNTPTRLVAKKGSNILPTAASLKGKTVGVEQGTIQETYAKTYWAPKGVNVQ
PYQNQDQVYADLLSGRLDATLQDAVQAEIGFLKTPRGAGYAFAGGDIDDPKTLGEGAGVGLRKEDTDLKAKIDGAIVAMR
KDGTYDKIAKKYFDFDVYGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=56.3706563706564, Blast_Score=296, Evalue=7e-82,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=54.9356223175966, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=39.6078431372549, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=34.7826086956522, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=32.0158102766798, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788228, Length=267, Percent_Identity=29.5880149812734, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27978; Mature: 27978

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLAALTVALLAVSAGGAYAKDWSTVRFGVDASYPPFESKGADGKLVGFDIDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHH
RRIAAKCVWVENDFDGLIPALKARKFDGVLSSMSMTPQRAEQIAFSDKLFNTPTRLVAKK
HHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHC
GSNILPTAASLKGKTVGVEQGTIQETYAKTYWAPKGVNVQPYQNQDQVYADLLSGRLDAT
CCCCCCCHHHCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHH
LQDAVQAEIGFLKTPRGAGYAFAGGDIDDPKTLGEGAGVGLRKEDTDLKAKIDGAIVAMR
HHHHHHHHHCCEECCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEECCEEEEEE
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKVLAALTVALLAVSAGGAYAKDWSTVRFGVDASYPPFESKGADGKLVGFDIDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHH
RRIAAKCVWVENDFDGLIPALKARKFDGVLSSMSMTPQRAEQIAFSDKLFNTPTRLVAKK
HHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHC
GSNILPTAASLKGKTVGVEQGTIQETYAKTYWAPKGVNVQPYQNQDQVYADLLSGRLDAT
CCCCCCCHHHCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHH
LQDAVQAEIGFLKTPRGAGYAFAGGDIDDPKTLGEGAGVGLRKEDTDLKAKIDGAIVAMR
HHHHHHHHHCCEECCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEECCEEEEEE
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]