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The map label for this gene is bcsZ [H]

Identifier: 238025441

GI number: 238025441

Start: 2642389

End: 2643624

Strand: Direct

Name: bcsZ [H]

Synonym: bglu_2g21310

Alternate gene names: 238025441

Gene position: 2642389-2643624 (Clockwise)

Preceding gene: 238025440

Following gene: 238025442

Centisome position: 93.46

GC content: 75.57

Gene sequence:

>1236_bases
ATGCGTGTCCTGTTCCAGCGCGGCTGCGGCCGCCCACGTCCTGCGCGGCGCCCGCTCGCCGCACTGACCACCGCCGTGGC
CGGCCTCGGCGCCTGCCTCGGCGCGGCCGGCGCCGGCCATGTCGAGGCCGCGCCGCCGGCCGCGGCCCACCCGCAGCCGG
CCGCCGCGCGCTGCGGCGACTGGAGCGCCTACCGCGCCTTCATCGCGCGCAACGTGCAGCCCGACGGCCGCGTGTTCGAC
GCGTCCACCCCGGCTCAGCAGTCGACCTCGGAAGGCCAGTCGTATGGCCTGTTCTTCGCGCTGGTGGCCAACGATCGCGA
CAACTTCGAGCGCATCCTCGGCTGGATCCGCGAGAATCTGGCCGGCGGCCATTTCAGCGCGCGCGACGTGCGCCTGCCCG
CCTGGCTGTGGGGCCGCAAGGGCGGCGCGTGGGGCGTGCTCGATCCGAACTCGGCATCCGACTCGGACCTCTGGATCGCC
TACGACCTGCTCGAGGCCGGGCGCCTCTGGCACGTGCCCGACTACACCGCGCTCGGCACCGCGCTGGCCGCGCAGGTCGT
GCGCCGCGAGGTGGTCGATCTCGCCGGGCTCGGCCCGATGCTGCTGCCCGGCCCGCAGGGTTTCGTCAACGGCGACGTCA
CGCGCCTGAATCCGAGCTACCTGCCGCTGCCGGTGCTGCGCGGGCTCGCCACCCAGCTCGGCGGCGACCCGTGGAACGCG
CTCGCGAGCAACGGCCTGAAGCTGGTGGCCGGCGCCTCGCCGCACGGCTTCGCCCCCGACTGGGCGGCCTGGCGCGCGGG
CCAGGTGGTGGTCGATCCGAAGACCGGCGACCTCGGCAGCTACGACGCGATCCGCGTCTATCTGTGGGCCGGCCTCGCGA
ATCCGGCCGATCCGCTCGCCAAGCCCTGGCTCGACGCCGTGCGCGGGATGGCCGCGCGCGTGGCGCAGACCGGCGTGCCG
CCCGAGAAGGTCGCGGTGGCGACCGGCGTCGGCAGCGGCGAAGGCCCGCTCAGCTACTGGGGCGCGCTCGCGCCGTACTT
CGCCGCGCTCGGCGACACGCGCGGCCTCGCGCTGGCACGCTCGCGCCTGACCGCGCTCGACGGCGAGGTGCCGGGCCACG
AGCCGGTCTACTACGACCGCGTGCTGGGCCTGTTCGGCGCGGGCGCCGTGGCCGGCCGCTACCACTTCGACGCGAGCGGC
CGGCTGGTGCCGGCCTGGAGCAAGGCATGCCGATGA

Upstream 100 bases:

>100_bases
CTGCTGGCGTTCAGCGGCGTTCTTGCGGCGATCGTGATCGCCGCGCTGTTCTATCGCGCGCTGCGCCGCATCGCTGCGCG
CCGACTTCAGGATTGAGGCG

Downstream 100 bases:

>100_bases
AACGCGCTCAGGCCGTCCGGCGCGCGCCGGCGCGCGCCCCCGCCCTGCTGCTGGCGCTCGCGCTGGCCGCCGGCGCCTTC
ACCCACGCCCCGCGCGCGGC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRVLFQRGCGRPRPARRPLAALTTAVAGLGACLGAAGAGHVEAAPPAAAHPQPAAARCGDWSAYRAFIARNVQPDGRVFD
ASTPAQQSTSEGQSYGLFFALVANDRDNFERILGWIRENLAGGHFSARDVRLPAWLWGRKGGAWGVLDPNSASDSDLWIA
YDLLEAGRLWHVPDYTALGTALAAQVVRREVVDLAGLGPMLLPGPQGFVNGDVTRLNPSYLPLPVLRGLATQLGGDPWNA
LASNGLKLVAGASPHGFAPDWAAWRAGQVVVDPKTGDLGSYDAIRVYLWAGLANPADPLAKPWLDAVRGMAARVAQTGVP
PEKVAVATGVGSGEGPLSYWGALAPYFAALGDTRGLALARSRLTALDGEVPGHEPVYYDRVLGLFGAGAVAGRYHFDASG
RLVPAWSKACR

Sequences:

>Translated_411_residues
MRVLFQRGCGRPRPARRPLAALTTAVAGLGACLGAAGAGHVEAAPPAAAHPQPAAARCGDWSAYRAFIARNVQPDGRVFD
ASTPAQQSTSEGQSYGLFFALVANDRDNFERILGWIRENLAGGHFSARDVRLPAWLWGRKGGAWGVLDPNSASDSDLWIA
YDLLEAGRLWHVPDYTALGTALAAQVVRREVVDLAGLGPMLLPGPQGFVNGDVTRLNPSYLPLPVLRGLATQLGGDPWNA
LASNGLKLVAGASPHGFAPDWAAWRAGQVVVDPKTGDLGSYDAIRVYLWAGLANPADPLAKPWLDAVRGMAARVAQTGVP
PEKVAVATGVGSGEGPLSYWGALAPYFAALGDTRGLALARSRLTALDGEVPGHEPVYYDRVLGLFGAGAVAGRYHFDASG
RLVPAWSKACR
>Mature_411_residues
MRVLFQRGCGRPRPARRPLAALTTAVAGLGACLGAAGAGHVEAAPPAAAHPQPAAARCGDWSAYRAFIARNVQPDGRVFD
ASTPAQQSTSEGQSYGLFFALVANDRDNFERILGWIRENLAGGHFSARDVRLPAWLWGRKGGAWGVLDPNSASDSDLWIA
YDLLEAGRLWHVPDYTALGTALAAQVVRREVVDLAGLGPMLLPGPQGFVNGDVTRLNPSYLPLPVLRGLATQLGGDPWNA
LASNGLKLVAGASPHGFAPDWAAWRAGQVVVDPKTGDLGSYDAIRVYLWAGLANPADPLAKPWLDAVRGMAARVAQTGVP
PEKVAVATGVGSGEGPLSYWGALAPYFAALGDTRGLALARSRLTALDGEVPGHEPVYYDRVLGLFGAGAVAGRYHFDASG
RLVPAWSKACR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=352, Percent_Identity=42.8977272727273, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 43233; Mature: 43233

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLFQRGCGRPRPARRPLAALTTAVAGLGACLGAAGAGHVEAAPPAAAHPQPAAARCGD
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCC
WSAYRAFIARNVQPDGRVFDASTPAQQSTSEGQSYGLFFALVANDRDNFERILGWIRENL
HHHHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
AGGHFSARDVRLPAWLWGRKGGAWGVLDPNSASDSDLWIAYDLLEAGRLWHVPDYTALGT
CCCCCCCCCEECCHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCEECCCCHHHHHH
ALAAQVVRREVVDLAGLGPMLLPGPQGFVNGDVTRLNPSYLPLPVLRGLATQLGGDPWNA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHH
LASNGLKLVAGASPHGFAPDWAAWRAGQVVVDPKTGDLGSYDAIRVYLWAGLANPADPLA
HHCCCEEEEECCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHH
KPWLDAVRGMAARVAQTGVPPEKVAVATGVGSGEGPLSYWGALAPYFAALGDTRGLALAR
HHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
SRLTALDGEVPGHEPVYYDRVLGLFGAGAVAGRYHFDASGRLVPAWSKACR
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHCCEEEECCCCCEECCHHHHCC
>Mature Secondary Structure
MRVLFQRGCGRPRPARRPLAALTTAVAGLGACLGAAGAGHVEAAPPAAAHPQPAAARCGD
CCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCC
WSAYRAFIARNVQPDGRVFDASTPAQQSTSEGQSYGLFFALVANDRDNFERILGWIRENL
HHHHHHHHHHCCCCCCCEEECCCCCHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHH
AGGHFSARDVRLPAWLWGRKGGAWGVLDPNSASDSDLWIAYDLLEAGRLWHVPDYTALGT
CCCCCCCCCEECCHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCEECCCCHHHHHH
ALAAQVVRREVVDLAGLGPMLLPGPQGFVNGDVTRLNPSYLPLPVLRGLATQLGGDPWNA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHH
LASNGLKLVAGASPHGFAPDWAAWRAGQVVVDPKTGDLGSYDAIRVYLWAGLANPADPLA
HHCCCEEEEECCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHH
KPWLDAVRGMAARVAQTGVPPEKVAVATGVGSGEGPLSYWGALAPYFAALGDTRGLALAR
HHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
SRLTALDGEVPGHEPVYYDRVLGLFGAGAVAGRYHFDASGRLVPAWSKACR
HHHHHHCCCCCCCCCHHHHHHHHHHCCCHHCCEEEECCCCCEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]