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The map label for this gene is citA [H]

Identifier: 238024746

GI number: 238024746

Start: 1771569

End: 1772939

Strand: Reverse

Name: citA [H]

Synonym: bglu_2g13830

Alternate gene names: 238024746

Gene position: 1772939-1771569 (Counterclockwise)

Preceding gene: 238024747

Following gene: 238024745

Centisome position: 62.71

GC content: 71.41

Gene sequence:

>1371_bases
ATGAGCCGCGCCACGCCCGCCCCGGCACACCAGGCAGCGCGGCCGGACGCGGCCGCGGCGCGTCTGTCGCCGGCGATGGT
GCGCCGCGCGATCTTCGCCTCGGTGCTCGGCAACGGCCTGGAATGGTTCGATTTCCTGATCTACGGCTATTTCTCGAAGA
TCATCGCGCAGGTGTTCTTCCCGGCCGGCAACGCGCTCGTCTCGCTGCTGCTGACGCTCGCGACCTTCGCGATCGGCTTC
GTGGTGCGGCCGATCGGCGGCATCCTGCTCGGCGTCTACGCCGACCGCGCGGGACGCAGCCGCGCGCTGTCGCTGCTGAT
CATCTCGATGGCGGCCAGCACGCTGCTGATGGGCCTCACGCCCGGCTACGCGCAGATCGGCTACGCGGCGCCCGCGCTGG
TGATCCTCGCGCGCGTGCTGCAGGGGCTCTCGGTGGGCGGCCAGTTCGCGACCGCATCGGCGATGCTGGTCGAGTACGCG
CCGCCCGGCCGCAGGATGTTCTACGGCAGCTTCAACATGACGGCGCAGTCGTTCGCGCTGCTGCTCTCGTCGGGCGCCGG
CTACCTGCTCACCACGCGGCTCTCGCACGACTCGCTGGCGGCATGGGGCTGGCGCGTGCCGTTCCTGTGCGGCGCGCTGG
CCGGCCCGCTCGGCTTCTACATCCGCCACCGGGTGGCCGAGTCGCCCGAGTTCCAGCAATTGCGCCGCGCGCAGCCGGAC
CCGCGCGCGACGGTGCCGCTTGCCGCGTTCTTCCGCGACAACGGCCGCGCCGCGCTCTGCGCGATGGGCGTGATCGCGGT
GGGGGCGGCCACCAACTACGTCTGGCACTCGTACCTCGCCGTCTACGTCGAGCGCCAGCTGCACCTGCCGCTGCAGGTCG
CGCTGCGCGGCGCGTTCCTCTCGGGCCTGCTGAACCTGTTCCTGTTCCCGCTGGCGGGGCGCCTGGCGGACCGCTTCGGC
GCGTACCGGCTGTTCTACGCGGTCACCATCGCCTGGATGATCTGCGCGTATCCGCTCTACCTGTTCGTGGTCGCGGAGCC
GTCGGCCTCGCGCCTGCTCGTCGCGCAACTCGTCGCGACGGTGTTTCTCGCGGCGATGTCGGGCGCGCACCCCGGCATGC
TCGCGACGCTGTTCCCGGTGCGGAGCCGCTCGGCCGGCGTCGCGCTCTCGTACAACCTCTCGGTCACGCTGTTCGGCGGC
CTGGCGCCGTTTACCGTCACCTGGCTGATCTCGCTGACGGGCAGCAGCCTGACACCCGCCTTCTATCTGATCTTTGCCGG
TTTCGTCTCGCTGGCGCTGGTCGGGTCGTGCCGTCGCGGGCACCCCGGGCTCGGCGTCGAGGCACCCGTCACGCCGAGGA
CGCCCGCTTGA

Upstream 100 bases:

>100_bases
AGCGCCTGCAGGTGGTGCCCGGCGTCGGCCACGACGGCGCGAAGATGCTGGGATCGGCCTGCGCGCTCGCGGCGATCTAC
GATACCGCGGCGTGCGGATC

Downstream 100 bases:

>100_bases
GCACCATGCCCCCCGACCTCGAGGGCAGCCACGACGAACGCGCGCCGAACGAACGGCCCGTCGACGTGATCGCCCCGCAG
CATCTGAGCGTCGCCACGCC

Product: major facilitator superfamily metabolite/H symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSRATPAPAHQAARPDAAAARLSPAMVRRAIFASVLGNGLEWFDFLIYGYFSKIIAQVFFPAGNALVSLLLTLATFAIGF
VVRPIGGILLGVYADRAGRSRALSLLIISMAASTLLMGLTPGYAQIGYAAPALVILARVLQGLSVGGQFATASAMLVEYA
PPGRRMFYGSFNMTAQSFALLLSSGAGYLLTTRLSHDSLAAWGWRVPFLCGALAGPLGFYIRHRVAESPEFQQLRRAQPD
PRATVPLAAFFRDNGRAALCAMGVIAVGAATNYVWHSYLAVYVERQLHLPLQVALRGAFLSGLLNLFLFPLAGRLADRFG
AYRLFYAVTIAWMICAYPLYLFVVAEPSASRLLVAQLVATVFLAAMSGAHPGMLATLFPVRSRSAGVALSYNLSVTLFGG
LAPFTVTWLISLTGSSLTPAFYLIFAGFVSLALVGSCRRGHPGLGVEAPVTPRTPA

Sequences:

>Translated_456_residues
MSRATPAPAHQAARPDAAAARLSPAMVRRAIFASVLGNGLEWFDFLIYGYFSKIIAQVFFPAGNALVSLLLTLATFAIGF
VVRPIGGILLGVYADRAGRSRALSLLIISMAASTLLMGLTPGYAQIGYAAPALVILARVLQGLSVGGQFATASAMLVEYA
PPGRRMFYGSFNMTAQSFALLLSSGAGYLLTTRLSHDSLAAWGWRVPFLCGALAGPLGFYIRHRVAESPEFQQLRRAQPD
PRATVPLAAFFRDNGRAALCAMGVIAVGAATNYVWHSYLAVYVERQLHLPLQVALRGAFLSGLLNLFLFPLAGRLADRFG
AYRLFYAVTIAWMICAYPLYLFVVAEPSASRLLVAQLVATVFLAAMSGAHPGMLATLFPVRSRSAGVALSYNLSVTLFGG
LAPFTVTWLISLTGSSLTPAFYLIFAGFVSLALVGSCRRGHPGLGVEAPVTPRTPA
>Mature_455_residues
SRATPAPAHQAARPDAAAARLSPAMVRRAIFASVLGNGLEWFDFLIYGYFSKIIAQVFFPAGNALVSLLLTLATFAIGFV
VRPIGGILLGVYADRAGRSRALSLLIISMAASTLLMGLTPGYAQIGYAAPALVILARVLQGLSVGGQFATASAMLVEYAP
PGRRMFYGSFNMTAQSFALLLSSGAGYLLTTRLSHDSLAAWGWRVPFLCGALAGPLGFYIRHRVAESPEFQQLRRAQPDP
RATVPLAAFFRDNGRAALCAMGVIAVGAATNYVWHSYLAVYVERQLHLPLQVALRGAFLSGLLNLFLFPLAGRLADRFGA
YRLFYAVTIAWMICAYPLYLFVVAEPSASRLLVAQLVATVFLAAMSGAHPGMLATLFPVRSRSAGVALSYNLSVTLFGGL
APFTVTWLISLTGSSLTPAFYLIFAGFVSLALVGSCRRGHPGLGVEAPVTPRTPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=31.5035799522673, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=29.8578199052133, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=33.8129496402878, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=31.3679245283019, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=25.9067357512953, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=234, Percent_Identity=23.5042735042735, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48580; Mature: 48449

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRATPAPAHQAARPDAAAARLSPAMVRRAIFASVLGNGLEWFDFLIYGYFSKIIAQVFF
CCCCCCCCCHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PAGNALVSLLLTLATFAIGFVVRPIGGILLGVYADRAGRSRALSLLIISMAASTLLMGLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PGYAQIGYAAPALVILARVLQGLSVGGQFATASAMLVEYAPPGRRMFYGSFNMTAQSFAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCCCEEEEECCCCHHHHHHH
LLSSGAGYLLTTRLSHDSLAAWGWRVPFLCGALAGPLGFYIRHRVAESPEFQQLRRAQPD
HHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
PRATVPLAAFFRDNGRAALCAMGVIAVGAATNYVWHSYLAVYVERQLHLPLQVALRGAFL
CCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SGLLNLFLFPLAGRLADRFGAYRLFYAVTIAWMICAYPLYLFVVAEPSASRLLVAQLVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
VFLAAMSGAHPGMLATLFPVRSRSAGVALSYNLSVTLFGGLAPFTVTWLISLTGSSLTPA
HHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHH
FYLIFAGFVSLALVGSCRRGHPGLGVEAPVTPRTPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRATPAPAHQAARPDAAAARLSPAMVRRAIFASVLGNGLEWFDFLIYGYFSKIIAQVFF
CCCCCCCCHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PAGNALVSLLLTLATFAIGFVVRPIGGILLGVYADRAGRSRALSLLIISMAASTLLMGLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
PGYAQIGYAAPALVILARVLQGLSVGGQFATASAMLVEYAPPGRRMFYGSFNMTAQSFAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCCCEEEEECCCCHHHHHHH
LLSSGAGYLLTTRLSHDSLAAWGWRVPFLCGALAGPLGFYIRHRVAESPEFQQLRRAQPD
HHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCC
PRATVPLAAFFRDNGRAALCAMGVIAVGAATNYVWHSYLAVYVERQLHLPLQVALRGAFL
CCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SGLLNLFLFPLAGRLADRFGAYRLFYAVTIAWMICAYPLYLFVVAEPSASRLLVAQLVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
VFLAAMSGAHPGMLATLFPVRSRSAGVALSYNLSVTLFGGLAPFTVTWLISLTGSSLTPA
HHHHHHCCCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHH
FYLIFAGFVSLALVGSCRRGHPGLGVEAPVTPRTPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]