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The map label for this gene is rihA [H]

Identifier: 238024552

GI number: 238024552

Start: 1471468

End: 1472502

Strand: Direct

Name: rihA [H]

Synonym: bglu_2g11590

Alternate gene names: 238024552

Gene position: 1471468-1472502 (Clockwise)

Preceding gene: 238024551

Following gene: 238024555

Centisome position: 52.04

GC content: 71.59

Gene sequence:

>1035_bases
ATGTCCAAGACAACCAGGCGGAGCTTTCTCATGACGACCACCGCACTCGCGGCGGGCACGCTGTTCCCGCTCACCAGTTT
CGCGGCCGGCGAGCGGCGCGATGTGATCATCGACACCGATCCGGGCCAGGACGACACCATCGCGATCCTGTTCGCACTGG
CCGCGCGCGACCGGCTCGACGTGCGCGCGCTGACGGCGGTGGCCGGCAACGTGCCGCTCGAACTGACCTCGCGCAACGCG
CGCATCATCCGCGACTGGGCCGGCCGCACGCAGACGCTGCCCGTCTACGCCGGCTGTGCGCGCCCGCTCGCGCGCGAACT
CGTGACGGCGGCCAACGTGCACGGCAAAACCGGCATCGAGGGCGTCGAGCTGCCCGAGCCGCTCGCCCCGCTCGCCCCGC
AGCACGCCGTGCAATACCTGATCGAGACGCTGCGCGCGGCGCCCAAGCGGAGCATCACGCTCTGCACGCTCGGCCCGCAG
ACCAACCTCGCCGCCGCGCTGGCCGAGGCGCCGGAGATCGGCGAGGCGCTGCGCGAGATCGTGATGATGGGCGGCGCCTT
CTTCGAGCGCGGCAACATCACGCCGGTGGCCGAGTTCAACGTCTACGTCGATCCCGAGGCGACGCGGGCGGTGCTGGCAA
GCGGCGTGCCGATCACCGTGGTACCGCGCGACGTGGCCGTGAAGGCCGCGATCACGCCGGCGCGCATCGCGCCGTTCCGC
GCGCTCGGCAACCGCTGCGGAAAGATCGTCGCCGACATCATGACGGCCGAGGTCGCGTACCAGCGGGCCACGGCCGGCGT
CGACTGGGCGCCGATGTACGACCCGAGCGCGACCGGCTACCTGATCGAGCCGTCGCTGTTCAAGGGCCGCGCCGTCAACG
TCGAGGTCGAGACCGGCAGCGCGCTGACGCTCGGCGAAACGGTGATCGACTGGACCGGGCGCACCGGGCGCAAGCCGAAT
GCGACCTGGATCACCGAGGTCGACGCGGACGGCTTCTACGCGGCGCTCCACCGGCGCATCGCCAGCCTGCCGTGA

Upstream 100 bases:

>100_bases
CGAAGCCGATCGCCCTCGCCTCGACGACGCGCGGGCAGCGGGCGGCCGGCGAGCGACGGACGGCGGCACCCTCGGCATGC
GTCACGTCTTCCGAACAACC

Downstream 100 bases:

>100_bases
TGGGGGACGCGGCCCGCGCGTGGCGCTCCACACGCCCGGACGCCCGAAGCTCGGCCGCTCAGGCTTCGGCCGGCCCGGGC
TGCGCGAGCCGCAGCAGATG

Product: Inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSKTTRRSFLMTTTALAAGTLFPLTSFAAGERRDVIIDTDPGQDDTIAILFALAARDRLDVRALTAVAGNVPLELTSRNA
RIIRDWAGRTQTLPVYAGCARPLARELVTAANVHGKTGIEGVELPEPLAPLAPQHAVQYLIETLRAAPKRSITLCTLGPQ
TNLAAALAEAPEIGEALREIVMMGGAFFERGNITPVAEFNVYVDPEATRAVLASGVPITVVPRDVAVKAAITPARIAPFR
ALGNRCGKIVADIMTAEVAYQRATAGVDWAPMYDPSATGYLIEPSLFKGRAVNVEVETGSALTLGETVIDWTGRTGRKPN
ATWITEVDADGFYAALHRRIASLP

Sequences:

>Translated_344_residues
MSKTTRRSFLMTTTALAAGTLFPLTSFAAGERRDVIIDTDPGQDDTIAILFALAARDRLDVRALTAVAGNVPLELTSRNA
RIIRDWAGRTQTLPVYAGCARPLARELVTAANVHGKTGIEGVELPEPLAPLAPQHAVQYLIETLRAAPKRSITLCTLGPQ
TNLAAALAEAPEIGEALREIVMMGGAFFERGNITPVAEFNVYVDPEATRAVLASGVPITVVPRDVAVKAAITPARIAPFR
ALGNRCGKIVADIMTAEVAYQRATAGVDWAPMYDPSATGYLIEPSLFKGRAVNVEVETGSALTLGETVIDWTGRTGRKPN
ATWITEVDADGFYAALHRRIASLP
>Mature_343_residues
SKTTRRSFLMTTTALAAGTLFPLTSFAAGERRDVIIDTDPGQDDTIAILFALAARDRLDVRALTAVAGNVPLELTSRNAR
IIRDWAGRTQTLPVYAGCARPLARELVTAANVHGKTGIEGVELPEPLAPLAPQHAVQYLIETLRAAPKRSITLCTLGPQT
NLAAALAEAPEIGEALREIVMMGGAFFERGNITPVAEFNVYVDPEATRAVLASGVPITVVPRDVAVKAAITPARIAPFRA
LGNRCGKIVADIMTAEVAYQRATAGVDWAPMYDPSATGYLIEPSLFKGRAVNVEVETGSALTLGETVIDWTGRTGRKPNA
TWITEVDADGFYAALHRRIASLP

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=42.7184466019417, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=38.2636655948553, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786213, Length=302, Percent_Identity=36.4238410596026, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17565698, Length=309, Percent_Identity=29.4498381877023, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=225, Percent_Identity=30.2222222222222, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=204, Percent_Identity=32.3529411764706, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24641837, Length=331, Percent_Identity=28.7009063444109, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583915, Length=206, Percent_Identity=31.0679611650485, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641839, Length=295, Percent_Identity=25.0847457627119, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 36713; Mature: 36581

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTTRRSFLMTTTALAAGTLFPLTSFAAGERRDVIIDTDPGQDDTIAILFALAARDRLD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCH
VRALTAVAGNVPLELTSRNARIIRDWAGRTQTLPVYAGCARPLARELVTAANVHGKTGIE
HHHHHHHHCCCCEEEECCCCEEEEHHCCCCEECEEHHHHHHHHHHHHHHHHCCCCCCCCC
GVELPEPLAPLAPQHAVQYLIETLRAAPKRSITLCTLGPQTNLAAALAEAPEIGEALREI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHH
VMMGGAFFERGNITPVAEFNVYVDPEATRAVLASGVPITVVPRDVAVKAAITPARIAPFR
HHHCCCEEECCCCCEEEEEEEEECCHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHH
ALGNRCGKIVADIMTAEVAYQRATAGVDWAPMYDPSATGYLIEPSLFKGRAVNVEVETGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCC
ALTLGETVIDWTGRTGRKPNATWITEVDADGFYAALHRRIASLP
EEEECCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKTTRRSFLMTTTALAAGTLFPLTSFAAGERRDVIIDTDPGQDDTIAILFALAARDRLD
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCH
VRALTAVAGNVPLELTSRNARIIRDWAGRTQTLPVYAGCARPLARELVTAANVHGKTGIE
HHHHHHHHCCCCEEEECCCCEEEEHHCCCCEECEEHHHHHHHHHHHHHHHHCCCCCCCCC
GVELPEPLAPLAPQHAVQYLIETLRAAPKRSITLCTLGPQTNLAAALAEAPEIGEALREI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHH
VMMGGAFFERGNITPVAEFNVYVDPEATRAVLASGVPITVVPRDVAVKAAITPARIAPFR
HHHCCCEEECCCCCEEEEEEEEECCHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHH
ALGNRCGKIVADIMTAEVAYQRATAGVDWAPMYDPSATGYLIEPSLFKGRAVNVEVETGS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCC
ALTLGETVIDWTGRTGRKPNATWITEVDADGFYAALHRRIASLP
EEEECCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA