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The map label for this gene is gsiB [H]

Identifier: 238024440

GI number: 238024440

Start: 1323153

End: 1324739

Strand: Direct

Name: gsiB [H]

Synonym: bglu_2g10360

Alternate gene names: 238024440

Gene position: 1323153-1324739 (Clockwise)

Preceding gene: 238024439

Following gene: 238024441

Centisome position: 46.8

GC content: 66.04

Gene sequence:

>1587_bases
ATGAGCGACGATATCGACAATGGCGGCGGCAAGGCCGGCTTCACGCGCCGCGACATGATGCGGATCATGGCGGCGGGCTG
CGTGATGTCCGCCGGCGCCGGCGGACTGCTGGTGGCGCCCCGGGCGGCCCACGCCGCGAGCGCGCCGAAGCGCGGCGGGA
AGATACGGGTTGCCAACGAATCCAGTTCGACGGCCGATACGCTGGACCCGGCCAAGGGGTCGAACAGCGCGGACTACATC
CGCTTCTTCATGTTCTACAGCGGCCTCACGCAGCTCGATCAGAGCCTGACGCCGCAGATGAATCTCGCCGAGTCGCTGCA
CACGACCGACGCGAAGACCTGGGTCATCAAGCTGCGCAAGGGCGTCACGTTCCACGACGGCAAGCCGGTCGGGCCGGCCG
ACGTGCTGTATTCCCTGATGCGCCACAAGAACGCGGCCACCGCCTCGAAGGTCAAGACGCTCGCCGACCAGTTCGCCGAG
GCGAAGGCGAGTGGCCCCGGCGAGGTCACGCTGACGCTCGCGAGCGCCAACGCCGACCTGCCGGTGATCCTCGCCACGCC
GCAGCTGGTGATCGTCAAGGACGGCACGACGGATTTCAGCGCCGGCATCGGCTGCGGCCCGTACAAGCTGAAGTCGTTCC
AGCCCGGCGTGTCGACCGTCGGGGTGCGCAACGACAGCTATTTCAAGGCGGGGATGCCCTACCTCGACGAGATCGAGCTG
ATCGGCATCAGCGACAGCGCGGCGCGCCTGAACGCGCTGCTGTCGGGCGACGTGCATCTGATCAACGCGGTCGATCCGCG
CTCGACCCAGCGGGTGGATGCCACGCCCGGCTATGCGCTGAAGGAAACCAAGTCGGGCCTCTACACCGACCTGATCATGC
GCAGCGAAAACCCGATCACCGCGAAGCCCGAGTTCGTCGAAGGGATGAAGTACCTGTTCGATCGCAATCAGATCCGCTCG
GCGGTGTTCCGCGGCTACGCGGTGGTCGGCAACGACCAGCCGATTCCGCCGGAGCACCGCTATTTCAACGCGTCGCTGCC
GCAGCGCCCATACGACCCGGACAAGGCGAAGTTCCTGTTCCAGAAGGCCGGCGCGCTCGGCACCACGCTGCCGCCGATCT
ATGCGACCTCGGATGCGAACGGGTCGATCGAGATGGCGGTGCTGCTGCAGCAGGCCGGGCAGAAGATCGGGCTGAACCTG
CAGGTCAATCGCGTCTCGTCGGACGGCTACTGGTCGAACCACTGGATGAAGCATCCGCTCGGCTTCGGCAACGTCAACCC
GCGCTCGAGCGCCGACGTGCTGTTTACGCAGTTCTTCAAGTCGGACGCGCCGTGGAACGAGTCGGGCTGGAAGAACGCGC
GCTTCGACCAGTTGCTGCTGGCCGCCCGCTCCGAAACCGACGACGCCAAACGCAAGCAGATGTACGGCGAGATGCAGGTG
ATCGTGTCGCAGCAGGGCCGGGTCGGGATTCCCGCCTTCATCAGCTTCCTCGACGGGTACGACAAGCGCATCGGCGGCCT
GGGCTCGATCCCCACCGGCCCGATGATGGGCAGCATGTTCGCCGAATACGTCTGGTGGAATGCCTGA

Upstream 100 bases:

>100_bases
TCGGCATGACGTTGCGCCCGCCACCAGCAGCCGGGCGCGCCGGCAGCGAAGCGCCACGCTGGACCCCAGGACCCACTTAC
CACAGTCAGGAGCAGTACGG

Downstream 100 bases:

>100_bases
GGCCGTCGCCCGCCGCCGCCACGCTCGACCTTCTCGGGAGTCTTGCTTCATGAACCGGACCCTCATCGGCCTGATCGGCC
GGCGCATCGCGGTGACCGCG

Product: family 5 extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSDDIDNGGGKAGFTRRDMMRIMAAGCVMSAGAGGLLVAPRAAHAASAPKRGGKIRVANESSSTADTLDPAKGSNSADYI
RFFMFYSGLTQLDQSLTPQMNLAESLHTTDAKTWVIKLRKGVTFHDGKPVGPADVLYSLMRHKNAATASKVKTLADQFAE
AKASGPGEVTLTLASANADLPVILATPQLVIVKDGTTDFSAGIGCGPYKLKSFQPGVSTVGVRNDSYFKAGMPYLDEIEL
IGISDSAARLNALLSGDVHLINAVDPRSTQRVDATPGYALKETKSGLYTDLIMRSENPITAKPEFVEGMKYLFDRNQIRS
AVFRGYAVVGNDQPIPPEHRYFNASLPQRPYDPDKAKFLFQKAGALGTTLPPIYATSDANGSIEMAVLLQQAGQKIGLNL
QVNRVSSDGYWSNHWMKHPLGFGNVNPRSSADVLFTQFFKSDAPWNESGWKNARFDQLLLAARSETDDAKRKQMYGEMQV
IVSQQGRVGIPAFISFLDGYDKRIGGLGSIPTGPMMGSMFAEYVWWNA

Sequences:

>Translated_528_residues
MSDDIDNGGGKAGFTRRDMMRIMAAGCVMSAGAGGLLVAPRAAHAASAPKRGGKIRVANESSSTADTLDPAKGSNSADYI
RFFMFYSGLTQLDQSLTPQMNLAESLHTTDAKTWVIKLRKGVTFHDGKPVGPADVLYSLMRHKNAATASKVKTLADQFAE
AKASGPGEVTLTLASANADLPVILATPQLVIVKDGTTDFSAGIGCGPYKLKSFQPGVSTVGVRNDSYFKAGMPYLDEIEL
IGISDSAARLNALLSGDVHLINAVDPRSTQRVDATPGYALKETKSGLYTDLIMRSENPITAKPEFVEGMKYLFDRNQIRS
AVFRGYAVVGNDQPIPPEHRYFNASLPQRPYDPDKAKFLFQKAGALGTTLPPIYATSDANGSIEMAVLLQQAGQKIGLNL
QVNRVSSDGYWSNHWMKHPLGFGNVNPRSSADVLFTQFFKSDAPWNESGWKNARFDQLLLAARSETDDAKRKQMYGEMQV
IVSQQGRVGIPAFISFLDGYDKRIGGLGSIPTGPMMGSMFAEYVWWNA
>Mature_527_residues
SDDIDNGGGKAGFTRRDMMRIMAAGCVMSAGAGGLLVAPRAAHAASAPKRGGKIRVANESSSTADTLDPAKGSNSADYIR
FFMFYSGLTQLDQSLTPQMNLAESLHTTDAKTWVIKLRKGVTFHDGKPVGPADVLYSLMRHKNAATASKVKTLADQFAEA
KASGPGEVTLTLASANADLPVILATPQLVIVKDGTTDFSAGIGCGPYKLKSFQPGVSTVGVRNDSYFKAGMPYLDEIELI
GISDSAARLNALLSGDVHLINAVDPRSTQRVDATPGYALKETKSGLYTDLIMRSENPITAKPEFVEGMKYLFDRNQIRSA
VFRGYAVVGNDQPIPPEHRYFNASLPQRPYDPDKAKFLFQKAGALGTTLPPIYATSDANGSIEMAVLLQQAGQKIGLNLQ
VNRVSSDGYWSNHWMKHPLGFGNVNPRSSADVLFTQFFKSDAPWNESGWKNARFDQLLLAARSETDDAKRKQMYGEMQVI
VSQQGRVGIPAFISFLDGYDKRIGGLGSIPTGPMMGSMFAEYVWWNA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=464, Percent_Identity=23.2758620689655, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789966, Length=482, Percent_Identity=22.6141078838174, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787762, Length=449, Percent_Identity=24.7216035634744, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=328, Percent_Identity=27.1341463414634, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57164; Mature: 57032

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDIDNGGGKAGFTRRDMMRIMAAGCVMSAGAGGLLVAPRAAHAASAPKRGGKIRVANE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCEEEEECC
SSSTADTLDPAKGSNSADYIRFFMFYSGLTQLDQSLTPQMNLAESLHTTDAKTWVIKLRK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEC
GVTFHDGKPVGPADVLYSLMRHKNAATASKVKTLADQFAEAKASGPGEVTLTLASANADL
CCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
PVILATPQLVIVKDGTTDFSAGIGCGPYKLKSFQPGVSTVGVRNDSYFKAGMPYLDEIEL
EEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEE
IGISDSAARLNALLSGDVHLINAVDPRSTQRVDATPGYALKETKSGLYTDLIMRSENPIT
EECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCEEEHEEECCCCCC
AKPEFVEGMKYLFDRNQIRSAVFRGYAVVGNDQPIPPEHRYFNASLPQRPYDPDKAKFLF
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHH
QKAGALGTTLPPIYATSDANGSIEMAVLLQQAGQKIGLNLQVNRVSSDGYWSNHWMKHPL
HHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GFGNVNPRSSADVLFTQFFKSDAPWNESGWKNARFDQLLLAARSETDDAKRKQMYGEMQV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEE
IVSQQGRVGIPAFISFLDGYDKRIGGLGSIPTGPMMGSMFAEYVWWNA
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDDIDNGGGKAGFTRRDMMRIMAAGCVMSAGAGGLLVAPRAAHAASAPKRGGKIRVANE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCEEEEECC
SSSTADTLDPAKGSNSADYIRFFMFYSGLTQLDQSLTPQMNLAESLHTTDAKTWVIKLRK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEC
GVTFHDGKPVGPADVLYSLMRHKNAATASKVKTLADQFAEAKASGPGEVTLTLASANADL
CCEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
PVILATPQLVIVKDGTTDFSAGIGCGPYKLKSFQPGVSTVGVRNDSYFKAGMPYLDEIEL
EEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEE
IGISDSAARLNALLSGDVHLINAVDPRSTQRVDATPGYALKETKSGLYTDLIMRSENPIT
EECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCEEEHEEECCCCCC
AKPEFVEGMKYLFDRNQIRSAVFRGYAVVGNDQPIPPEHRYFNASLPQRPYDPDKAKFLF
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHH
QKAGALGTTLPPIYATSDANGSIEMAVLLQQAGQKIGLNLQVNRVSSDGYWSNHWMKHPL
HHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GFGNVNPRSSADVLFTQFFKSDAPWNESGWKNARFDQLLLAARSETDDAKRKQMYGEMQV
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHEE
IVSQQGRVGIPAFISFLDGYDKRIGGLGSIPTGPMMGSMFAEYVWWNA
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA