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The map label for this gene is gca [H]

Identifier: 238024268

GI number: 238024268

Start: 1030931

End: 1031974

Strand: Reverse

Name: gca [H]

Synonym: bglu_2g08510

Alternate gene names: 238024268

Gene position: 1031974-1030931 (Counterclockwise)

Preceding gene: 238024269

Following gene: 238024249

Centisome position: 36.5

GC content: 66.19

Gene sequence:

>1044_bases
ATGAACTCTGCACGCAAGGCGATCATCACCGGCATCTCCGGGCAGGACGGCGCCTATCTGGCCAAGCTGCTGCTCGACGG
CGGCTATCAGGTTACCGGCACCTACCGGCGCACCAGCTCGGTCAACTTCTGGCGCATCGCCGAGCTCGGCGTGGCCGATC
ATCCGAACCTGACGCTGGTCGAGCACGACCTGACCGATCCCGGGTCGAGCCTGCGGCTGATCGAGCGCGTCCAGCCCGAC
GAGTTGTACAATCTGGCGGCGCAGAGCTTCGTCGGCGTCTCGTTCGACCAGCCGATGACGACCGCCGGCGTGACCGGGCT
CGGCACGCTGAACCTGCTCGAGGCGATCCGCGCCGTGAGCCCGCGCACGCGCTTCTATCAGGCCTCGACCTCGGAGATGT
TCGGCAAGGTGCAGGCCATTCCGCAGATCGAATCGACGCCGTTCTACCCGCGCAGCCCGTATGGCGTCGCCAAGCTGTTC
GCCCACTGGATGACCGTCAACTATCGCGAGTCCTACGGCATCTTCGCGGCCAGCGGGATCCTGTTCAACCACGAATCACC
GCTGCGCGGGCGCGAGTTCGTCACGCGCAAGATCACCGACACGGTCGCCAAGATCAAGCTCGGCAAGGCCACCCGGCTCG
AGCTCGGCAACCTCGACGCGAAACGCGACTGGGGCTTCGCGCTCGAATACGTGGACGGCATGCGGCGCATGCTGCAGGTG
GACGAGCCCGACACCTTCGTGCTCGCCACCAACCGCACCGAGACGGTCCGCGACTTCGCGCAGATGGCGTTCGCCGCGGC
CGGCTACCAGCTCGAATGGCGCGGCAAGGAAGAGCACGAGCGCGGCATCGACACGGCTACCGGCCGCGTCCTGGTGGAGG
TGAACCCGCGCTTCTACCGGCCGGCCGAGGTCGATCTGCTGATCGGCTGCGCCGACAAGGCAAGGGAAAAGCTGGACTGG
CAGCCCCGCACGACACTCGAACAGCTCTGCCAGATGATGGTCGAGGCCGATCTCACGCGGAACCTCCATCACGAAACCTG
CTGA

Upstream 100 bases:

>100_bases
TCACCTGGTTCGGCTTCGAACGGCGCCTCACGCGCTCTGCGCCCGCGCCGTCGCGGCCGGCCGTGCCCCGGCCTTCCACT
CGCTCCATCAGGAACCCGTT

Downstream 100 bases:

>100_bases
TTCGAACCGGGTCCGCCGCGCGCTCGTGACGGGCCTGGCCGGCTTCCCGGGCCGCTTGTCGCGACGAGCCTCGCGGCGGC
CGGCTGTGCAGTGCGGAGGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNSARKAIITGISGQDGAYLAKLLLDGGYQVTGTYRRTSSVNFWRIAELGVADHPNLTLVEHDLTDPGSSLRLIERVQPD
ELYNLAAQSFVGVSFDQPMTTAGVTGLGTLNLLEAIRAVSPRTRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLF
AHWMTVNYRESYGIFAASGILFNHESPLRGREFVTRKITDTVAKIKLGKATRLELGNLDAKRDWGFALEYVDGMRRMLQV
DEPDTFVLATNRTETVRDFAQMAFAAAGYQLEWRGKEEHERGIDTATGRVLVEVNPRFYRPAEVDLLIGCADKAREKLDW
QPRTTLEQLCQMMVEADLTRNLHHETC

Sequences:

>Translated_347_residues
MNSARKAIITGISGQDGAYLAKLLLDGGYQVTGTYRRTSSVNFWRIAELGVADHPNLTLVEHDLTDPGSSLRLIERVQPD
ELYNLAAQSFVGVSFDQPMTTAGVTGLGTLNLLEAIRAVSPRTRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLF
AHWMTVNYRESYGIFAASGILFNHESPLRGREFVTRKITDTVAKIKLGKATRLELGNLDAKRDWGFALEYVDGMRRMLQV
DEPDTFVLATNRTETVRDFAQMAFAAAGYQLEWRGKEEHERGIDTATGRVLVEVNPRFYRPAEVDLLIGCADKAREKLDW
QPRTTLEQLCQMMVEADLTRNLHHETC
>Mature_347_residues
MNSARKAIITGISGQDGAYLAKLLLDGGYQVTGTYRRTSSVNFWRIAELGVADHPNLTLVEHDLTDPGSSLRLIERVQPD
ELYNLAAQSFVGVSFDQPMTTAGVTGLGTLNLLEAIRAVSPRTRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLF
AHWMTVNYRESYGIFAASGILFNHESPLRGREFVTRKITDTVAKIKLGKATRLELGNLDAKRDWGFALEYVDGMRRMLQV
DEPDTFVLATNRTETVRDFAQMAFAAAGYQLEWRGKEEHERGIDTATGRVLVEVNPRFYRPAEVDLLIGCADKAREKLDW
QPRTTLEQLCQMMVEADLTRNLHHETC

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=340, Percent_Identity=52.6470588235294, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=22.6865671641791, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788366, Length=356, Percent_Identity=50, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI48994969, Length=231, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=343, Percent_Identity=53.3527696793003, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901790, Length=343, Percent_Identity=53.3527696793003, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901786, Length=343, Percent_Identity=53.3527696793003, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17539422, Length=343, Percent_Identity=53.3527696793003, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI133901788, Length=343, Percent_Identity=53.3527696793003, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=53.3923303834808, Blast_Score=352, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24158427, Length=339, Percent_Identity=50.4424778761062, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38978; Mature: 38978

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSARKAIITGISGQDGAYLAKLLLDGGYQVTGTYRRTSSVNFWRIAELGVADHPNLTLV
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCEEEE
EHDLTDPGSSLRLIERVQPDELYNLAAQSFVGVSFDQPMTTAGVTGLGTLNLLEAIRAVS
ECCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHCC
PRTRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLFAHWMTVNYRESYGIFAASGI
CCHHEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCEEEECCE
LFNHESPLRGREFVTRKITDTVAKIKLGKATRLELGNLDAKRDWGFALEYVDGMRRMLQV
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
DEPDTFVLATNRTETVRDFAQMAFAAAGYQLEWRGKEEHERGIDTATGRVLVEVNPRFYR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCEEEEEECCCEEC
PAEVDLLIGCADKAREKLDWQPRTTLEQLCQMMVEADLTRNLHHETC
CCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNSARKAIITGISGQDGAYLAKLLLDGGYQVTGTYRRTSSVNFWRIAELGVADHPNLTLV
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCCCEEEE
EHDLTDPGSSLRLIERVQPDELYNLAAQSFVGVSFDQPMTTAGVTGLGTLNLLEAIRAVS
ECCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHCC
PRTRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLFAHWMTVNYRESYGIFAASGI
CCHHEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCEEEECCE
LFNHESPLRGREFVTRKITDTVAKIKLGKATRLELGNLDAKRDWGFALEYVDGMRRMLQV
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
DEPDTFVLATNRTETVRDFAQMAFAAAGYQLEWRGKEEHERGIDTATGRVLVEVNPRFYR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCEEEEEECCCEEC
PAEVDLLIGCADKAREKLDWQPRTTLEQLCQMMVEADLTRNLHHETC
CCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]