Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 238024257

GI number: 238024257

Start: 1019310

End: 1020140

Strand: Direct

Name: truA [H]

Synonym: bglu_2g08400

Alternate gene names: 238024257

Gene position: 1019310-1020140 (Clockwise)

Preceding gene: 238024256

Following gene: 238024258

Centisome position: 36.05

GC content: 70.4

Gene sequence:

>831_bases
ATGCGCATCGCGCTCGGCATCCAGTACGACGGCGCGGCGTTCCACGGCTGGCAGTCGCAGCCGCACGGCAACACCGTGCA
GGACACGCTGGAGCGGGCCCTGAAGGCGTTCGCGCGGGTGCCGCTGCAGACCGTCGTGGCGGGGCGCACCGACACCGGCG
TCCACGGGCTCGGCCAGGTGGTCCATTTCGACACCGAACTCGACCGCACCGCATTCTCCTGGGTGCGCGGCACCAATGCG
TTCCTGCCGCCGAGCGTCGCGGTGCAGTGGGCCAAGCCGATGCCCGAGGCGTTCCATGCCCGCTTCGCCGCGTTCGAGCG
CACCTACTACTACGCGCTCTACGTGCATCCGGTGCGCTCGCCGATGCTGGCCGCGCGCGCGGGCTGGGTGCACACGCCGC
TCGACGTGGAGGCGATGCGCGCCGCCGCGGCGCATCTGATCGGCGAACACGATTTCTCGTCGTTCCGCTCCTCCGAGTGC
CAGTCGAAGACGCCCGTCAAGCATCTGTACCAGATCGACATCCGGCCGTCGGGCAGCTTCATCCATTTCCGTTTTCGCGC
CAACGCGTTCCTGCATCACATGGTGCGCAACCTGATGGGCTGCCTGATCGCGGTGGGGCGCGGCCGCTATCCGGCCGACT
GGCTCGACGAGGTGCTGGCCGCGCGCAATCGCGACCGGGCCGCGCCGACCTTCGCGCCCGACGGCCTCTATCTGGCCCAT
GTCGGCTATCCTGACAGCTTTGCCGTGCCGCCCGCGCAGCTCGGCAGCGTGCCGTGGAGCACGGTCTGGGCCGACCTCGA
CGCGCCGCCGGCCCCGCCAGCGAACCCCTGA

Upstream 100 bases:

>100_bases
ACGGCGCCCGCATGCTGCTGAACGAGGTGATCGACGCGGGCCGCGCCGGCACCCGCGACGCCGCGCGCACGCTGCTCGCG
AAACTCGCCGACGCGACGTG

Downstream 100 bases:

>100_bases
TGCGGGCGGCCACGCCCGCGCCCCGAGCCGATCCCTTGTTGACGATGAGCGATACTCTGACTTCCCCCGGCGCCGCGCCG
CGCCTTCGCACCCGCATCAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRIALGIQYDGAAFHGWQSQPHGNTVQDTLERALKAFARVPLQTVVAGRTDTGVHGLGQVVHFDTELDRTAFSWVRGTNA
FLPPSVAVQWAKPMPEAFHARFAAFERTYYYALYVHPVRSPMLAARAGWVHTPLDVEAMRAAAAHLIGEHDFSSFRSSEC
QSKTPVKHLYQIDIRPSGSFIHFRFRANAFLHHMVRNLMGCLIAVGRGRYPADWLDEVLAARNRDRAAPTFAPDGLYLAH
VGYPDSFAVPPAQLGSVPWSTVWADLDAPPAPPANP

Sequences:

>Translated_276_residues
MRIALGIQYDGAAFHGWQSQPHGNTVQDTLERALKAFARVPLQTVVAGRTDTGVHGLGQVVHFDTELDRTAFSWVRGTNA
FLPPSVAVQWAKPMPEAFHARFAAFERTYYYALYVHPVRSPMLAARAGWVHTPLDVEAMRAAAAHLIGEHDFSSFRSSEC
QSKTPVKHLYQIDIRPSGSFIHFRFRANAFLHHMVRNLMGCLIAVGRGRYPADWLDEVLAARNRDRAAPTFAPDGLYLAH
VGYPDSFAVPPAQLGSVPWSTVWADLDAPPAPPANP
>Mature_276_residues
MRIALGIQYDGAAFHGWQSQPHGNTVQDTLERALKAFARVPLQTVVAGRTDTGVHGLGQVVHFDTELDRTAFSWVRGTNA
FLPPSVAVQWAKPMPEAFHARFAAFERTYYYALYVHPVRSPMLAARAGWVHTPLDVEAMRAAAAHLIGEHDFSSFRSSEC
QSKTPVKHLYQIDIRPSGSFIHFRFRANAFLHHMVRNLMGCLIAVGRGRYPADWLDEVLAARNRDRAAPTFAPDGLYLAH
VGYPDSFAVPPAQLGSVPWSTVWADLDAPPAPPANP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=30.1818181818182, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=46.0629921259843, Blast_Score=243, Evalue=1e-65,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=29.3729372937294, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30563; Mature: 30563

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIQYDGAAFHGWQSQPHGNTVQDTLERALKAFARVPLQTVVAGRTDTGVHGLGQV
CEEEEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHE
VHFDTELDRTAFSWVRGTNAFLPPSVAVQWAKPMPEAFHARFAAFERTYYYALYVHPVRS
EEECCHHHHHHHHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHEEEEEEEEECCCC
PMLAARAGWVHTPLDVEAMRAAAAHLIGEHDFSSFRSSECQSKTPVKHLYQIDIRPSGSF
CHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCE
IHFRFRANAFLHHMVRNLMGCLIAVGRGRYPADWLDEVLAARNRDRAAPTFAPDGLYLAH
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VGYPDSFAVPPAQLGSVPWSTVWADLDAPPAPPANP
ECCCCCCCCCHHHHCCCCHHHHEECCCCCCCCCCCC
>Mature Secondary Structure
MRIALGIQYDGAAFHGWQSQPHGNTVQDTLERALKAFARVPLQTVVAGRTDTGVHGLGQV
CEEEEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHE
VHFDTELDRTAFSWVRGTNAFLPPSVAVQWAKPMPEAFHARFAAFERTYYYALYVHPVRS
EEECCHHHHHHHHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHEEEEEEEEECCCC
PMLAARAGWVHTPLDVEAMRAAAAHLIGEHDFSSFRSSECQSKTPVKHLYQIDIRPSGSF
CHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCE
IHFRFRANAFLHHMVRNLMGCLIAVGRGRYPADWLDEVLAARNRDRAAPTFAPDGLYLAH
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
VGYPDSFAVPPAQLGSVPWSTVWADLDAPPAPPANP
ECCCCCCCCCHHHHCCCCHHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA