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The map label for this gene is vnfA [H]

Identifier: 238024155

GI number: 238024155

Start: 874171

End: 875142

Strand: Direct

Name: vnfA [H]

Synonym: bglu_2g07330

Alternate gene names: 238024155

Gene position: 874171-875142 (Clockwise)

Preceding gene: 238024154

Following gene: 238024171

Centisome position: 30.92

GC content: 71.71

Gene sequence:

>972_bases
ATGAACCAGTGTTCCCGTCTCAACATGCTCGGCTCGTCCGACATCATGGAGGCGGTCAGGCGCCAGATCGAGAAGATCGC
GGGCGTCGACGTGCCGGTAACCGTGCTCGGCGAGACCGGCACGGGCAAGGAGCTCGCGGTGCGGGCCATCCATTACCTGA
GCGGGCGCCGCCAGGCGCCGTTCGTTCCCGTCAATTGCGGCGCGCTGCCGGAGGCGCTGGTCGAAAGCGAGCTGTTCGGC
CATGAACGCGGCGCGTTCACCGACGCCAAGCTCGTCAGCGACGGCCTGATCGGCGAGGCCGAGGGCGGCACGCTGTTCCT
CGACGAAATCGACGCGCTCGGCCTGAAGGCGCAGGCCGCGCTGCTGCGCTTCCTGCAGGACGGCAGCTACCGGCGCGTGG
GCGGCACGCGGATCCGCCAGGCGGACGTGCGCGTGGTGGTGGCCAGCAACGCCGATCTGGCCGCGCTGGCCGAGACGCGG
CAGTTCCGGCGCGACCTGCTGTACCGCCTCGACGTGGTGATGCTGCGGATTCCGCCCGTGCGCGAGCGCGGCGGCGACGT
GGTCGAACTGGCCGAGGCGTTTCTCGACCGGCTGCGCACGCGCTACGCGAAGCCCGGGATGCGGCTTCACGAGGACTCCT
ACCCGCATCTGCGCAGCCACGCGTGGCCGGGCAACGTGCGCGAGCTCGAGAACGCCGTGCATCGCGCCGTGCTGCTCAGC
GAGGACGGTTTCGTGCGGCTCGGCGGCGCGGCTGGCGCGGCCGGCTCGGAGGGCATCGCGCACGCTGCCGCGCGCCCGTT
GAAGAGCGGGCGCGCGGCGGCGATCGCCAGCTTCGAGATCGATTACCTGCGGCGGCTGCTGGCCGAGGCGGACGGCAATC
TCTCGCAGGCCGCGCGGCTCGCGGGCGAGGAGCGCAGCGCGTTCGGCAAGCTGGTGCGCAAGCACGGCCTGGTGCGCGAT
CGCTCGAACTGA

Upstream 100 bases:

>100_bases
CAGGCATGTGCAGCCCTACCGCCCGACAATAAAACCGCTCCGCCGCGGCGTCCTTCCAGCGCCCGCCGTCGCAGGGCAAT
CGACCAGCTTCGGAGTGCGT

Downstream 100 bases:

>100_bases
GCCGGGCGTGGCGGTTGAGGTAAGCTTTCGGCTTGACGAATTCTTGACGGCAAGGTGCGCTTCATGAATGACGCGTTGGT
TCAGCACGTCGCCGCCCTAA

Product: Sigma 54-dependent transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNQCSRLNMLGSSDIMEAVRRQIEKIAGVDVPVTVLGETGTGKELAVRAIHYLSGRRQAPFVPVNCGALPEALVESELFG
HERGAFTDAKLVSDGLIGEAEGGTLFLDEIDALGLKAQAALLRFLQDGSYRRVGGTRIRQADVRVVVASNADLAALAETR
QFRRDLLYRLDVVMLRIPPVRERGGDVVELAEAFLDRLRTRYAKPGMRLHEDSYPHLRSHAWPGNVRELENAVHRAVLLS
EDGFVRLGGAAGAAGSEGIAHAAARPLKSGRAAAIASFEIDYLRRLLAEADGNLSQAARLAGEERSAFGKLVRKHGLVRD
RSN

Sequences:

>Translated_323_residues
MNQCSRLNMLGSSDIMEAVRRQIEKIAGVDVPVTVLGETGTGKELAVRAIHYLSGRRQAPFVPVNCGALPEALVESELFG
HERGAFTDAKLVSDGLIGEAEGGTLFLDEIDALGLKAQAALLRFLQDGSYRRVGGTRIRQADVRVVVASNADLAALAETR
QFRRDLLYRLDVVMLRIPPVRERGGDVVELAEAFLDRLRTRYAKPGMRLHEDSYPHLRSHAWPGNVRELENAVHRAVLLS
EDGFVRLGGAAGAAGSEGIAHAAARPLKSGRAAAIASFEIDYLRRLLAEADGNLSQAARLAGEERSAFGKLVRKHGLVRD
RSN
>Mature_323_residues
MNQCSRLNMLGSSDIMEAVRRQIEKIAGVDVPVTVLGETGTGKELAVRAIHYLSGRRQAPFVPVNCGALPEALVESELFG
HERGAFTDAKLVSDGLIGEAEGGTLFLDEIDALGLKAQAALLRFLQDGSYRRVGGTRIRQADVRVVVASNADLAALAETR
QFRRDLLYRLDVVMLRIPPVRERGGDVVELAEAFLDRLRTRYAKPGMRLHEDSYPHLRSHAWPGNVRELENAVHRAVLLS
EDGFVRLGGAAGAAGSEGIAHAAARPLKSGRAAAIASFEIDYLRRLLAEADGNLSQAARLAGEERSAFGKLVRKHGLVRD
RSN

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=38.4858044164038, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788905, Length=306, Percent_Identity=41.1764705882353, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=46.7811158798283, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789087, Length=240, Percent_Identity=45, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=40.3785488958991, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=40.1929260450161, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=38.1987577639752, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790299, Length=330, Percent_Identity=35.1515151515151, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1789233, Length=241, Percent_Identity=43.9834024896266, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=43.5483870967742, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787583, Length=316, Percent_Identity=35.4430379746835, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=30.7958477508651, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=33.0543933054393, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35090; Mature: 35090

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQCSRLNMLGSSDIMEAVRRQIEKIAGVDVPVTVLGETGTGKELAVRAIHYLSGRRQAP
CCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
FVPVNCGALPEALVESELFGHERGAFTDAKLVSDGLIGEAEGGTLFLDEIDALGLKAQAA
EEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEECHHHHCHHHHHH
LLRFLQDGSYRRVGGTRIRQADVRVVVASNADLAALAETRQFRRDLLYRLDVVMLRIPPV
HHHHHHCCCEEECCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCH
RERGGDVVELAEAFLDRLRTRYAKPGMRLHEDSYPHLRSHAWPGNVRELENAVHRAVLLS
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHEEC
EDGFVRLGGAAGAAGSEGIAHAAARPLKSGRAAAIASFEIDYLRRLLAEADGNLSQAARL
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AGEERSAFGKLVRKHGLVRDRSN
HCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQCSRLNMLGSSDIMEAVRRQIEKIAGVDVPVTVLGETGTGKELAVRAIHYLSGRRQAP
CCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
FVPVNCGALPEALVESELFGHERGAFTDAKLVSDGLIGEAEGGTLFLDEIDALGLKAQAA
EEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEEEECHHHHCHHHHHH
LLRFLQDGSYRRVGGTRIRQADVRVVVASNADLAALAETRQFRRDLLYRLDVVMLRIPPV
HHHHHHCCCEEECCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCH
RERGGDVVELAEAFLDRLRTRYAKPGMRLHEDSYPHLRSHAWPGNVRELENAVHRAVLLS
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHEEC
EDGFVRLGGAAGAAGSEGIAHAAARPLKSGRAAAIASFEIDYLRRLLAEADGNLSQAARL
CCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AGEERSAFGKLVRKHGLVRDRSN
HCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]