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The map label for this gene is paaF [C]

Identifier: 238024092

GI number: 238024092

Start: 785856

End: 786689

Strand: Reverse

Name: paaF [C]

Synonym: bglu_2g06560

Alternate gene names: 238024092

Gene position: 786689-785856 (Counterclockwise)

Preceding gene: 238024093

Following gene: 238024091

Centisome position: 27.82

GC content: 63.31

Gene sequence:

>834_bases
ATGAGCAGTCAGGCAGAGGAACAAACGGTTGTTTTCAGCATCGAGGACGGCGTGGCATGGGTGAAGTTCAACCGGCCCGA
AAAGCGTAACGCAATGAGCCCGGCGCTGAACCGGCGCATGATGCAGGTGCTCGACGAACTGGAATTCCATCCGGACGTGG
GCGTGCTCGTGCTGACCGGCGAAGGCACCGCGTGGACGGCCGGTATGGATCTCCAGGAATACTTCCGCGACACGGAAGCC
CATGGTCTGGCCGGGACGCGCAAGGCACAGCGCGAGAGCTACGGCTGGTGGCGACGCCTGCGCTGGTACCAGAAGCCCAC
CATCGCCATGATCAACGGCTGGTGCTTCGGTGGCGGCTACGGTCCGCTGTTCGCGTGCGATCTCGCGTTCTGCGCCGAGG
AAGCCCGCTTTGGCCTTTCGGAGATCAACTGGGGCATCCTGCCAGGAGGCGGCGCATCGAAGGTCGCGACCGAGCTGCTT
TCGTTCCGACGCGCGATGTATCACGCGATGCTGGGCGAGAACATCGACGGCCGCAAGGCCGAAGAATGGGGCCTGGTGAA
CGAGGCGGTGCCCGCCGCGCAACTGCGCACGCGGGTGGTGGAAGTCGCGCAGGCGCTGCTGAAGAAGAATCCGGTCGCCC
TGAAGGCAACCAAGGATGCCATCCGCCGTGTCCGCGAAATGACTTATGAGAACGCCGAAGACTTCCTCGTGCGAGCTCAG
GAGGCCGCCAACAGCTACGACGACGGGCGCAAGGAGGGGCTCAAACAGTTCCTTGACGACAAGACCTACAAGCCGGGCCT
TGGCACTTACGACCTCAAGCGACAGCGTACCTGA

Upstream 100 bases:

>100_bases
AGGATGCCGTCCCGCAGTATCCGTTCTAGGCGGCAACTAGGCGCCCGCGCTGATATGGGAGCCCGAAAAAACTTTTTCAC
TTTCGATCAAGGTGCAGGTA

Downstream 100 bases:

>100_bases
GCACGGGAGCCCGTTGACATGAACCTGATCCAGTCTGGAACCTGGCCTGCGGCGACAGGCCAGGCCGTGGCGCGCCTGCT
GCGACCGTCATCGATTGCCA

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSSQAEEQTVVFSIEDGVAWVKFNRPEKRNAMSPALNRRMMQVLDELEFHPDVGVLVLTGEGTAWTAGMDLQEYFRDTEA
HGLAGTRKAQRESYGWWRRLRWYQKPTIAMINGWCFGGGYGPLFACDLAFCAEEARFGLSEINWGILPGGGASKVATELL
SFRRAMYHAMLGENIDGRKAEEWGLVNEAVPAAQLRTRVVEVAQALLKKNPVALKATKDAIRRVREMTYENAEDFLVRAQ
EAANSYDDGRKEGLKQFLDDKTYKPGLGTYDLKRQRT

Sequences:

>Translated_277_residues
MSSQAEEQTVVFSIEDGVAWVKFNRPEKRNAMSPALNRRMMQVLDELEFHPDVGVLVLTGEGTAWTAGMDLQEYFRDTEA
HGLAGTRKAQRESYGWWRRLRWYQKPTIAMINGWCFGGGYGPLFACDLAFCAEEARFGLSEINWGILPGGGASKVATELL
SFRRAMYHAMLGENIDGRKAEEWGLVNEAVPAAQLRTRVVEVAQALLKKNPVALKATKDAIRRVREMTYENAEDFLVRAQ
EAANSYDDGRKEGLKQFLDDKTYKPGLGTYDLKRQRT
>Mature_276_residues
SSQAEEQTVVFSIEDGVAWVKFNRPEKRNAMSPALNRRMMQVLDELEFHPDVGVLVLTGEGTAWTAGMDLQEYFRDTEAH
GLAGTRKAQRESYGWWRRLRWYQKPTIAMINGWCFGGGYGPLFACDLAFCAEEARFGLSEINWGILPGGGASKVATELLS
FRRAMYHAMLGENIDGRKAEEWGLVNEAVPAAQLRTRVVEVAQALLKKNPVALKATKDAIRRVREMTYENAEDFLVRAQE
AANSYDDGRKEGLKQFLDDKTYKPGLGTYDLKRQRT

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=28.2786885245902, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=26.8, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI45643119, Length=233, Percent_Identity=29.6137339055794, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260275230, Length=233, Percent_Identity=29.6137339055794, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260274832, Length=233, Percent_Identity=29.6137339055794, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI31542718, Length=214, Percent_Identity=27.5700934579439, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=24.4444444444444, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=24.4444444444444, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=31.1538461538462, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=27.906976744186, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI221142681, Length=217, Percent_Identity=29.9539170506912, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=28.3582089552239, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=23.7735849056604, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=25, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=29.0076335877863, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=30.4761904761905, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=27.3092369477912, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=26.5217391304348, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=234, Percent_Identity=22.6495726495727, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=178, Percent_Identity=29.7752808988764, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=178, Percent_Identity=29.7752808988764, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=178, Percent_Identity=29.7752808988764, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=217, Percent_Identity=24.4239631336406, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=28.7449392712551, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=28.7449392712551, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19920382, Length=240, Percent_Identity=27.9166666666667, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=22.6053639846743, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=26.7379679144385, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=276, Percent_Identity=23.5507246376812, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24653139, Length=214, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24650670, Length=284, Percent_Identity=24.2957746478873, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31282; Mature: 31151

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQAEEQTVVFSIEDGVAWVKFNRPEKRNAMSPALNRRMMQVLDELEFHPDVGVLVLTG
CCCCCCCCEEEEEECCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEC
EGTAWTAGMDLQEYFRDTEAHGLAGTRKAQRESYGWWRRLRWYQKPTIAMINGWCFGGGY
CCCEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCC
GPLFACDLAFCAEEARFGLSEINWGILPGGGASKVATELLSFRRAMYHAMLGENIDGRKA
CHHHHHHHHHHHHHHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EEWGLVNEAVPAAQLRTRVVEVAQALLKKNPVALKATKDAIRRVREMTYENAEDFLVRAQ
HHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
EAANSYDDGRKEGLKQFLDDKTYKPGLGTYDLKRQRT
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSQAEEQTVVFSIEDGVAWVKFNRPEKRNAMSPALNRRMMQVLDELEFHPDVGVLVLTG
CCCCCCCEEEEEECCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEC
EGTAWTAGMDLQEYFRDTEAHGLAGTRKAQRESYGWWRRLRWYQKPTIAMINGWCFGGGY
CCCEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCCC
GPLFACDLAFCAEEARFGLSEINWGILPGGGASKVATELLSFRRAMYHAMLGENIDGRKA
CHHHHHHHHHHHHHHHCCHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EEWGLVNEAVPAAQLRTRVVEVAQALLKKNPVALKATKDAIRRVREMTYENAEDFLVRAQ
HHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHH
EAANSYDDGRKEGLKQFLDDKTYKPGLGTYDLKRQRT
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA