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The map label for this gene is oprM [H]

Identifier: 238024074

GI number: 238024074

Start: 761130

End: 762650

Strand: Reverse

Name: oprM [H]

Synonym: bglu_2g06350

Alternate gene names: 238024074

Gene position: 762650-761130 (Counterclockwise)

Preceding gene: 238024075

Following gene: 238024073

Centisome position: 26.97

GC content: 71.14

Gene sequence:

>1521_bases
GTGCCGGCCGGCGCGGCGGCCCTGCTGCTCGCCGCCTGCACGATGGCGCCGCATTACCAGCGGCCCGCCGCCCCCGTGTC
GGACCGCTTCCCGTCCGACGGCGTCTACGCGACGCAGCCGGGCGCGGACGCCGCGCACAGCGCCGATGGCCGGGCCGCCG
CCGACCTCGGCTGGCGCGACTTCTTCGCCGATCAGCGCCTGCAGGCGCTGATCGAGATCGCGCTGAAGCACAACCGCGAT
CTGCGTGTCGCGGTGCTCAACATCGAGGCGGCGCGCGCGCAATACCGGATCACGCGGGCGGGGCTGTTGCCGAGCCTGGA
TGGCAGCGGCACCGGCAACATCCAGCGGCTGCCGGCCGGCGTGTCGACCACCGGGGCGGCGGACATGACGCGAACCTACA
ACGTCGGCGCGTCGGCCTCGTGGGAGCTGGACCTGTTCGGCCGGGTCCGGAGCCTGAAGGACGCGGCCCTGGCGCAGTAC
CTGTCGACGGCCCAGGCGCGCAAGGCGGCGGAAATCTCGCTGATCTCGCAGGTGGCGGACCAGTACCTGACGCTGCTGTC
GACGGACGACCTGCTGAAGGTCACCGAGGACACGCTGAAGACGGCCCGGGATTCGTACCACCTGACCAAGCTGCAGTTCG
ACAACGGCACCGGCTCGGAACTGGACCTGCGCCAGGCACAGACGGTGGTCGAGCAGGCGCTGGCGAACCAGCAGGCCCAG
GCGCGCGCTCGCGCGCAAGCCTTGAACGCGTTGGCGCTGCTGATCGGCCAGCCGCTGCCCGACGATCTGCCGGCCGGCCT
GCCGCTGGATTCGCAGGGCCTGCTGGCCGACATCCCGGCCGGCCTGCCGTCGGATCTGCTGACGCGCCGCCCGGACATCA
TGGAAGCCGAGCAGACGCTGCTGGCAGCCAATGCGAACCTCGGGGCGGCCCGCGCCGCCTTCTTTCCGAGGATCTCGCTG
ACGGCGGCGTTCGGCACGGCGAGCCCGACCCTGGGCGGCCTCTTCAAGGCCGGCACGGCGGCCTGGTCGTTCGCGCCGAA
CCTCGCCCTGCCGATCTTCGAGGGCGGTTCGAACCTCGCGAACCTGGATCTGGCGCACGTGCGCAAGCGCATCGAGATCG
CGAACTACGAGAAGGCGATCCAGACGGCGTTCCGGGAAGTGTCGGACGGGCTGGCGGCACGCGGCACGTATGACCAGCAG
ATCGCCGCGCTGTCGCGCAACGAAGCGGCGCAACGGCGCCGCTACGCGCTGTCGGATCTGCGTTACCGCAACGGCGCGGA
CAGCTACCTGGCGGTGCTGACGGCCCAGACGGATCTGTACACCGCGCAGACCACGCTGATCTCGGCGCGCCTGGCGCGCT
GGACCAACCTGGTGGACCTGTATCGCGCGCTCGGCGGCGGCTGGATCGAGCACAACGGCGATCAGCCGCGCGAAGCCGAT
GCACCACCAGCAGGGGCGAGTGAAAAAGGCGCGGCGCCGGACGTGGGGCAAAATGATTCCGCCACGCCGGAGCGCCGCTG
A

Upstream 100 bases:

>100_bases
GCGAGCGCCTGCTCGCCACCCAGCATTGAGACGCCTCGTGTCCGCCTTCGCTCGCCTATTCGTCCGCTGCTCCGTTCCCG
CGCCCACCCTGAAGCGGTCC

Downstream 100 bases:

>100_bases
ATCGAGGCCCGCGGCCGGCTCACCGCGCAGAGTACGGTGACGGGGTGGCGCCCGTTCGCGGCGGGGTACGCATGGATCGC
CGATCATCGCGAACCGTCCG

Product: putative outer membrane protein OprM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPAGAAALLLAACTMAPHYQRPAAPVSDRFPSDGVYATQPGADAAHSADGRAAADLGWRDFFADQRLQALIEIALKHNRD
LRVAVLNIEAARAQYRITRAGLLPSLDGSGTGNIQRLPAGVSTTGAADMTRTYNVGASASWELDLFGRVRSLKDAALAQY
LSTAQARKAAEISLISQVADQYLTLLSTDDLLKVTEDTLKTARDSYHLTKLQFDNGTGSELDLRQAQTVVEQALANQQAQ
ARARAQALNALALLIGQPLPDDLPAGLPLDSQGLLADIPAGLPSDLLTRRPDIMEAEQTLLAANANLGAARAAFFPRISL
TAAFGTASPTLGGLFKAGTAAWSFAPNLALPIFEGGSNLANLDLAHVRKRIEIANYEKAIQTAFREVSDGLAARGTYDQQ
IAALSRNEAAQRRRYALSDLRYRNGADSYLAVLTAQTDLYTAQTTLISARLARWTNLVDLYRALGGGWIEHNGDQPREAD
APPAGASEKGAAPDVGQNDSATPERR

Sequences:

>Translated_506_residues
MPAGAAALLLAACTMAPHYQRPAAPVSDRFPSDGVYATQPGADAAHSADGRAAADLGWRDFFADQRLQALIEIALKHNRD
LRVAVLNIEAARAQYRITRAGLLPSLDGSGTGNIQRLPAGVSTTGAADMTRTYNVGASASWELDLFGRVRSLKDAALAQY
LSTAQARKAAEISLISQVADQYLTLLSTDDLLKVTEDTLKTARDSYHLTKLQFDNGTGSELDLRQAQTVVEQALANQQAQ
ARARAQALNALALLIGQPLPDDLPAGLPLDSQGLLADIPAGLPSDLLTRRPDIMEAEQTLLAANANLGAARAAFFPRISL
TAAFGTASPTLGGLFKAGTAAWSFAPNLALPIFEGGSNLANLDLAHVRKRIEIANYEKAIQTAFREVSDGLAARGTYDQQ
IAALSRNEAAQRRRYALSDLRYRNGADSYLAVLTAQTDLYTAQTTLISARLARWTNLVDLYRALGGGWIEHNGDQPREAD
APPAGASEKGAAPDVGQNDSATPERR
>Mature_505_residues
PAGAAALLLAACTMAPHYQRPAAPVSDRFPSDGVYATQPGADAAHSADGRAAADLGWRDFFADQRLQALIEIALKHNRDL
RVAVLNIEAARAQYRITRAGLLPSLDGSGTGNIQRLPAGVSTTGAADMTRTYNVGASASWELDLFGRVRSLKDAALAQYL
STAQARKAAEISLISQVADQYLTLLSTDDLLKVTEDTLKTARDSYHLTKLQFDNGTGSELDLRQAQTVVEQALANQQAQA
RARAQALNALALLIGQPLPDDLPAGLPLDSQGLLADIPAGLPSDLLTRRPDIMEAEQTLLAANANLGAARAAFFPRISLT
AAFGTASPTLGGLFKAGTAAWSFAPNLALPIFEGGSNLANLDLAHVRKRIEIANYEKAIQTAFREVSDGLAARGTYDQQI
AALSRNEAAQRRRYALSDLRYRNGADSYLAVLTAQTDLYTAQTTLISARLARWTNLVDLYRALGGGWIEHNGDQPREADA
PPAGASEKGAAPDVGQNDSATPERR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=464, Percent_Identity=41.8103448275862, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI2367348, Length=428, Percent_Identity=28.0373831775701, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53892; Mature: 53761

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGAAALLLAACTMAPHYQRPAAPVSDRFPSDGVYATQPGADAAHSADGRAAADLGWRD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCHHH
FFADQRLQALIEIALKHNRDLRVAVLNIEAARAQYRITRAGLLPSLDGSGTGNIQRLPAG
HHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC
VSTTGAADMTRTYNVGASASWELDLFGRVRSLKDAALAQYLSTAQARKAAEISLISQVAD
CCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLTLLSTDDLLKVTEDTLKTARDSYHLTKLQFDNGTGSELDLRQAQTVVEQALANQQAQ
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
ARARAQALNALALLIGQPLPDDLPAGLPLDSQGLLADIPAGLPSDLLTRRPDIMEAEQTL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCHHHHHHHH
LAANANLGAARAAFFPRISLTAAFGTASPTLGGLFKAGTAAWSFAPNLALPIFEGGSNLA
HHCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCEEEEECCCCCCC
NLDLAHVRKRIEIANYEKAIQTAFREVSDGLAARGTYDQQIAALSRNEAAQRRRYALSDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYRNGADSYLAVLTAQTDLYTAQTTLISARLARWTNLVDLYRALGGGWIEHNGDQPREAD
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
APPAGASEKGAAPDVGQNDSATPERR
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PAGAAALLLAACTMAPHYQRPAAPVSDRFPSDGVYATQPGADAAHSADGRAAADLGWRD
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCHHH
FFADQRLQALIEIALKHNRDLRVAVLNIEAARAQYRITRAGLLPSLDGSGTGNIQRLPAG
HHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC
VSTTGAADMTRTYNVGASASWELDLFGRVRSLKDAALAQYLSTAQARKAAEISLISQVAD
CCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLTLLSTDDLLKVTEDTLKTARDSYHLTKLQFDNGTGSELDLRQAQTVVEQALANQQAQ
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
ARARAQALNALALLIGQPLPDDLPAGLPLDSQGLLADIPAGLPSDLLTRRPDIMEAEQTL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCHHHHHHHH
LAANANLGAARAAFFPRISLTAAFGTASPTLGGLFKAGTAAWSFAPNLALPIFEGGSNLA
HHCCCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCEEEEECCCCCCC
NLDLAHVRKRIEIANYEKAIQTAFREVSDGLAARGTYDQQIAALSRNEAAQRRRYALSDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYRNGADSYLAVLTAQTDLYTAQTTLISARLARWTNLVDLYRALGGGWIEHNGDQPREAD
HHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCC
APPAGASEKGAAPDVGQNDSATPERR
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]