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The map label for this gene is purU [H]

Identifier: 238024039

GI number: 238024039

Start: 714135

End: 715013

Strand: Reverse

Name: purU [H]

Synonym: bglu_2g05930

Alternate gene names: 238024039

Gene position: 715013-714135 (Counterclockwise)

Preceding gene: 238024047

Following gene: 238024028

Centisome position: 25.29

GC content: 68.03

Gene sequence:

>879_bases
ATGACCGGCTCCGAACGCAATTCCCGATTCCTGCTGACGCTGTCGTGCCCGAGCGCGGCCGGCCAGGTGGCCGCCGTGGT
CGGCGTGCTCGACCGCCACCAGTGCTACGTCGACGAGCTGACCGTGTTCGACGACGACCTGAGCGGGCGCTTCTTCGTGC
GCTGCGCGTTCCATCCGGCCGCCGCGGGCAGCACGCCCGACATCGACGCGCTGCGCGCCGAGTTCGCGCCGATCGCGGCG
CGCTTCGACATGCAATGGGCAATCCACGACGCCGGCGCGCGCCCGAAGGTGATGATCCTCGTCTCGAAGCTCGAGCACTG
CCTAGCGGACCTGCTGTTCCGCTGGAAGATGGGCGAGCTGAAGATGGACATCGTCGCGATCGCCTCGAACCACCCCGATC
TCGAACCGCTCGCGGCCCAGCACGGGCTGCCGTTCCGCCATTTCCCGATCACGCCCGAGACCAAGGCGCAGCAGGAGGCG
CAGTGGCTCGACCTGTTCGAATCGAGCGGCGCCGAGCTGGTGATCCTCGCGCGCTACATGCAGGTGCTCTCGCCCGGCAC
GAGCGCGCGGCTGGCCAACCGCGCGATCAACATCCACCACTCGTTCCTGCCCGGCTTCAAGGGTGCCAAGCCCTACCACC
AGGCGCACGCGCGCGGCGTGAAACTGATCGGCGCCACCGCTCATTTCGTGACCGACGATCTCGACGAAGGCCCCATCATC
GAGCAGGTGGTGGAGCGCGTCGATCACGCCTATCGGCCCGAGCAGTTGCTGACGGTGGGCCGCGACGTCGAATCCATCAC
GCTCGCGCGCGCCGTGAAGGCGTTCATCGAGCGCCGCGTGTTCCTGAACGGCGACCGCACCGTGGTGTTCGGGCAGTAA

Upstream 100 bases:

>100_bases
TTCGATTCTATCCACCGAGGCGCGCAACTGGTCTACTGGAAACCGGCCGCTTCGGTGCGGCCGCGTGCGGCGCGCCGCGC
CGCCGTGATCCGAGGAAAGC

Downstream 100 bases:

>100_bases
TTCGGGCAGCAAGGCCCCCCGCCTTCCGGCGGCCGTCACGCCGTGTGCCGGCCACCGGCCCGCGATTCACGATGTTTCCT
CGTCTCTCACCCTGAAGGCC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTGSERNSRFLLTLSCPSAAGQVAAVVGVLDRHQCYVDELTVFDDDLSGRFFVRCAFHPAAAGSTPDIDALRAEFAPIAA
RFDMQWAIHDAGARPKVMILVSKLEHCLADLLFRWKMGELKMDIVAIASNHPDLEPLAAQHGLPFRHFPITPETKAQQEA
QWLDLFESSGAELVILARYMQVLSPGTSARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
EQVVERVDHAYRPEQLLTVGRDVESITLARAVKAFIERRVFLNGDRTVVFGQ

Sequences:

>Translated_292_residues
MTGSERNSRFLLTLSCPSAAGQVAAVVGVLDRHQCYVDELTVFDDDLSGRFFVRCAFHPAAAGSTPDIDALRAEFAPIAA
RFDMQWAIHDAGARPKVMILVSKLEHCLADLLFRWKMGELKMDIVAIASNHPDLEPLAAQHGLPFRHFPITPETKAQQEA
QWLDLFESSGAELVILARYMQVLSPGTSARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
EQVVERVDHAYRPEQLLTVGRDVESITLARAVKAFIERRVFLNGDRTVVFGQ
>Mature_291_residues
TGSERNSRFLLTLSCPSAAGQVAAVVGVLDRHQCYVDELTVFDDDLSGRFFVRCAFHPAAAGSTPDIDALRAEFAPIAAR
FDMQWAIHDAGARPKVMILVSKLEHCLADLLFRWKMGELKMDIVAIASNHPDLEPLAAQHGLPFRHFPITPETKAQQEAQ
WLDLFESSGAELVILARYMQVLSPGTSARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QVVERVDHAYRPEQLLTVGRDVESITLARAVKAFIERRVFLNGDRTVVFGQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=39.2982456140351, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1788846, Length=150, Percent_Identity=29.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=160, Percent_Identity=36.875, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32313; Mature: 32182

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSERNSRFLLTLSCPSAAGQVAAVVGVLDRHQCYVDELTVFDDDLSGRFFVRCAFHPA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHEEEECCCCCCEEEEEEEECCC
AAGSTPDIDALRAEFAPIAARFDMQWAIHDAGARPKVMILVSKLEHCLADLLFRWKMGEL
CCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
KMDIVAIASNHPDLEPLAAQHGLPFRHFPITPETKAQQEAQWLDLFESSGAELVILARYM
EEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
QVLSPGTSARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
HHHCCCCHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCHHHHCCCCCCCCHH
EQVVERVDHAYRPEQLLTVGRDVESITLARAVKAFIERRVFLNGDRTVVFGQ
HHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECC
>Mature Secondary Structure 
TGSERNSRFLLTLSCPSAAGQVAAVVGVLDRHQCYVDELTVFDDDLSGRFFVRCAFHPA
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHEEEECCCCCCEEEEEEEECCC
AAGSTPDIDALRAEFAPIAARFDMQWAIHDAGARPKVMILVSKLEHCLADLLFRWKMGEL
CCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
KMDIVAIASNHPDLEPLAAQHGLPFRHFPITPETKAQQEAQWLDLFESSGAELVILARYM
EEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
QVLSPGTSARLANRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPII
HHHCCCCHHHHHHHHEEEEHHHCCCCCCCCCHHHHHHCCEEEEECCHHHHCCCCCCCCHH
EQVVERVDHAYRPEQLLTVGRDVESITLARAVKAFIERRVFLNGDRTVVFGQ
HHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]