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The map label for this gene is stcD [H]

Identifier: 238024031

GI number: 238024031

Start: 702317

End: 704380

Strand: Direct

Name: stcD [H]

Synonym: bglu_2g05850

Alternate gene names: 238024031

Gene position: 702317-704380 (Clockwise)

Preceding gene: 238024030

Following gene: 238024032

Centisome position: 24.84

GC content: 68.46

Gene sequence:

>2064_bases
ATGCGTTATCCGAACCTGTTCAAGCCCTTGACGCTCAACCAGCTCACGCTGCGCAACCGCATCGTCAGCACCGCGCACGC
GGAGGTCTATGCGGAGCCGGGCGGCCTGCCGGGCGACCGGTACATCCGCTACTACGAAGAGAAGGCCAAGGGCGGCGTGG
GCCTGGCCGTCTGCGGCGGCTCCAGCCCGGTGTCGATCGACAGCCCGCAGGGCTGGTGGAAGTCGGTCAATCTGTCGACC
GACCGGATCGTCGATCCGCTCGCGCGGCTGGCCGAGGCAATGCACCGGCACGGCGCGAAGATCATGATCCAGGCCACCCA
CATGGGCCGCCGCTCGGCCTTCCACGGCGAGCACTGGCCGCACCTGATGACGCCCTCGGGCGTGCGCGAGCCGGTGCATC
GCGGCAACGCGAAGATCATCGAGGTCGAGGAGATCCGCCGCATCATCGGCGATTTCGCGGCCGCCGCGAAGCGCGTGAAG
GACGCCGGCATGGACGGCATCGAGATCTCGGCCGCCCACCAGCATTTGATCGACCAGTTCTGGAGCCCGCGCACCAACTT
CCGCACCGACGAATGGGGCGGCAGCCTCGAGAACCGCCTGCGCTTCGGCGTGGAAGTGCTCACGGCGGTGCGCGAGGCGG
TGGGCCGCGACTTCTGCGTGGGCCTGCGCATGTGCGGCGACGAATTCCACGAGGACGGGCTCGACCACGAGCAGCTCAGG
GAGATCGCGCAGGCGATGTCCGAGACCGGCCTGATCGACTATCTCGGCGTGATCGGCTCGGGCGCCGACACCCACAACAC
GCTCGCCAACTGCATGCCGCCGATGGCGCTGCCGCCCGAGCCGTTCGTGCATCTGGCGGCCGGCATCAAGTCGGTGGTGA
AGATCCCGGTGATGCACGCGCAGAGCATTCGCGACGCGGGCCAGGCCGAGCGGCTGCTGGCCAGCGGCATGGTCGACCTG
GTCGGCATGACGCGCGCGCAGATCGCCGATCCGCACATGGTGATCAAGATCCGCGACGGCCGCGAGGACGAGATCAAGCA
GTGCGTGGGCGCGAACTACTGCATCGACCGCCAGTACAACGGCCTCGACGTGCTCTGCGTGCAGAACGCGGCCACCTCGC
GCGAGGCAACCATGCCGCACGTGATCGAGAAGTCGCGCGGGCCGAAGCGCAAGGTGGTGGTGGTGGGCGCCGGCCCCGCG
GGGCTGGAGGCCGCGCGCGTGGCGAAGCTGCGCGGCCACGACGTGGTGCTGTTCGAGAAGAACACGGAAGTGGGCGGCCA
GGTCATGATCGCGGCCAAGGCACCGCAGCGCGAGCAGATGGCCGGCATCGTGCGCTGGTTCGACATGGAAACCAAGCGCC
TCGGCGTGGACCGCCGGCTCGGCGTGGCCGCCGACGAGAAGCAGATCATGGCCGAGAAGCCCGACATCGTGGTGCTGGCC
ACCGGCGGCGCCAGCTTCACCGGCCAGGTGCCGGCCTGGGGCGTGGCCGAGGGGCTCGCGGTCAGCTCGTGGGACATCCT
CACGGGCCGCGTCGAGCCCAAGCAGAACGTGCTGGTCTACGACGGCGTGAGCACGCACGCGGGTGCGGGCGTGGCCGATT
TCATCTCGAGCCGCGGCGCGCAGGTGGAGATCGTCACGCCCGACGTGAAGGTGGCCGACGACTGCGGCGGCACCACCTTC
CCGATCTTCTACCGCCGGCTCTACGCGCAGGGCGTGATCCATACGCCGAACTACTGGCTCGATCGCGTCTACGAGGACAA
CGGCAAGATCATCGCCGTGCTGCGCAACGAGTACACCGAGGAGCAGGAGGAGCGCGCGGTCGACCAGGTGGTGATCGAGA
ACGGCTCGACGCCGAACGACCAGCTCTACTGGACGCTCAAGCCCGAATCGGTCAACCGCGGGCAGGTGGACGTCCACAAG
CTGTTCGCCGCCGAGCCGCAGCCGAGCCTGTCGGAAGAACTCGGCAACGGGCGCTTCCTGCTGTTCCGCGTCGGCGACTG
CATCTCGATGCACAACATCCACGGCGCGATCTACGACGCGCTGCGCCTCGTGAAGGACTTCTGA

Upstream 100 bases:

>100_bases
TCCCGATCACCAGGGCCACTGCGTGTTCGACGTGTCGCCGCTGGCGGCCTGATTCCCGATCCCCCACCCGACTAGAGTCC
CCTCCCAGAGGTCGCCCGCG

Downstream 100 bases:

>100_bases
CCATGAATCCGGCTTTCCTGATTACCGCGCTGCTGTGGCTGTCGGTGGCGGGGCTCGCGTTCGCCGTGGCCAGGCGCTCG
GCCTACTGGCGCGGCGGGCG

Product: FAD/FMN-binding oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MRYPNLFKPLTLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGGSSPVSIDSPQGWWKSVNLST
DRIVDPLARLAEAMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIGDFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRTDEWGGSLENRLRFGVEVLTAVREAVGRDFCVGLRMCGDEFHEDGLDHEQLR
EIAQAMSETGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLASGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHVIEKSRGPKRKVVVVGAGPA
GLEAARVAKLRGHDVVLFEKNTEVGGQVMIAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADEKQIMAEKPDIVVLA
TGGASFTGQVPAWGVAEGLAVSSWDILTGRVEPKQNVLVYDGVSTHAGAGVADFISSRGAQVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEDNGKIIAVLRNEYTEEQEERAVDQVVIENGSTPNDQLYWTLKPESVNRGQVDVHK
LFAAEPQPSLSEELGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF

Sequences:

>Translated_687_residues
MRYPNLFKPLTLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGGSSPVSIDSPQGWWKSVNLST
DRIVDPLARLAEAMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIGDFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRTDEWGGSLENRLRFGVEVLTAVREAVGRDFCVGLRMCGDEFHEDGLDHEQLR
EIAQAMSETGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLASGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHVIEKSRGPKRKVVVVGAGPA
GLEAARVAKLRGHDVVLFEKNTEVGGQVMIAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADEKQIMAEKPDIVVLA
TGGASFTGQVPAWGVAEGLAVSSWDILTGRVEPKQNVLVYDGVSTHAGAGVADFISSRGAQVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEDNGKIIAVLRNEYTEEQEERAVDQVVIENGSTPNDQLYWTLKPESVNRGQVDVHK
LFAAEPQPSLSEELGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF
>Mature_687_residues
MRYPNLFKPLTLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGGSSPVSIDSPQGWWKSVNLST
DRIVDPLARLAEAMHRHGAKIMIQATHMGRRSAFHGEHWPHLMTPSGVREPVHRGNAKIIEVEEIRRIIGDFAAAAKRVK
DAGMDGIEISAAHQHLIDQFWSPRTNFRTDEWGGSLENRLRFGVEVLTAVREAVGRDFCVGLRMCGDEFHEDGLDHEQLR
EIAQAMSETGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLASGMVDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCVQNAATSREATMPHVIEKSRGPKRKVVVVGAGPA
GLEAARVAKLRGHDVVLFEKNTEVGGQVMIAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADEKQIMAEKPDIVVLA
TGGASFTGQVPAWGVAEGLAVSSWDILTGRVEPKQNVLVYDGVSTHAGAGVADFISSRGAQVEIVTPDVKVADDCGGTTF
PIFYRRLYAQGVIHTPNYWLDRVYEDNGKIIAVLRNEYTEEQEERAVDQVVIENGSTPNDQLYWTLKPESVNRGQVDVHK
LFAAEPQPSLSEELGNGRFLLFRVGDCISMHNIHGAIYDALRLVKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=669, Percent_Identity=31.2406576980568, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1787939, Length=359, Percent_Identity=26.4623955431755, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17566914, Length=277, Percent_Identity=31.0469314079422, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=349, Percent_Identity=26.3610315186246, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=348, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=344, Percent_Identity=22.6744186046512, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=336, Percent_Identity=25.5952380952381, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=342, Percent_Identity=25.4385964912281, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=348, Percent_Identity=26.7241379310345, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=242, Percent_Identity=30.1652892561983, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325086, Length=246, Percent_Identity=28.8617886178862, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 75543; Mature: 75543

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYPNLFKPLTLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSTDRIVDPLARLAEAMHRHGAKIMIQATHMGRRSAFHGEHWP
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMTPSGVREPVHRGNAKIIEVEEIRRIIGDFAAAAKRVKDAGMDGIEISAAHQHLIDQF
CCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRTNFRTDEWGGSLENRLRFGVEVLTAVREAVGRDFCVGLRMCGDEFHEDGLDHEQLR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMSETGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKIPVMHA
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLASGMVDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCC
GLDVLCVQNAATSREATMPHVIEKSRGPKRKVVVVGAGPAGLEAARVAKLRGHDVVLFEK
CEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEC
NTEVGGQVMIAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADEKQIMAEKPDIVVLA
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHCCCCCEEEEE
TGGASFTGQVPAWGVAEGLAVSSWDILTGRVEPKQNVLVYDGVSTHAGAGVADFISSRGA
ECCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHCCCC
QVEIVTPDVKVADDCGGTTFPIFYRRLYAQGVIHTPNYWLDRVYEDNGKIIAVLRNEYTE
EEEEECCCCEEECCCCCCHHHHHHHHHHHCCCEECCCHHHHHEECCCCCEEEEECCCCCH
EQEERAVDQVVIENGSTPNDQLYWTLKPESVNRGQVDVHKLFAAEPQPSLSEELGNGRFL
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCEE
LFRVGDCISMHNIHGAIYDALRLVKDF
EEEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYPNLFKPLTLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAVCGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSTDRIVDPLARLAEAMHRHGAKIMIQATHMGRRSAFHGEHWP
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMTPSGVREPVHRGNAKIIEVEEIRRIIGDFAAAAKRVKDAGMDGIEISAAHQHLIDQF
CCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRTNFRTDEWGGSLENRLRFGVEVLTAVREAVGRDFCVGLRMCGDEFHEDGLDHEQLR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMSETGLIDYLGVIGSGADTHNTLANCMPPMALPPEPFVHLAAGIKSVVKIPVMHA
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLASGMVDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCC
GLDVLCVQNAATSREATMPHVIEKSRGPKRKVVVVGAGPAGLEAARVAKLRGHDVVLFEK
CEEEEEECCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEC
NTEVGGQVMIAAKAPQREQMAGIVRWFDMETKRLGVDRRLGVAADEKQIMAEKPDIVVLA
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHCCCCCEEEEE
TGGASFTGQVPAWGVAEGLAVSSWDILTGRVEPKQNVLVYDGVSTHAGAGVADFISSRGA
ECCCCCCCCCCCCCCCCCCEECCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHCCCC
QVEIVTPDVKVADDCGGTTFPIFYRRLYAQGVIHTPNYWLDRVYEDNGKIIAVLRNEYTE
EEEEECCCCEEECCCCCCHHHHHHHHHHHCCCEECCCHHHHHEECCCCCEEEEECCCCCH
EQEERAVDQVVIENGSTPNDQLYWTLKPESVNRGQVDVHKLFAAEPQPSLSEELGNGRFL
HHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCEE
LFRVGDCISMHNIHGAIYDALRLVKDF
EEEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]