| Definition | Burkholderia glumae BGR1 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_012721 |
| Length | 2,827,333 |
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The map label for this gene is metE [H]
Identifier: 238023944
GI number: 238023944
Start: 588640
End: 590925
Strand: Reverse
Name: metE [H]
Synonym: bglu_2g04930
Alternate gene names: 238023944
Gene position: 590925-588640 (Counterclockwise)
Preceding gene: 339717967
Following gene: 238023943
Centisome position: 20.9
GC content: 69.73
Gene sequence:
>2286_bases ATGGTCACCACTCACAACCTCGGCTTCCCTCGCATCGGCGCGAAACGCGAACTGAAATTCGCCCTCGAGCGCTACTGGAA GGGCGAGTCCTCGCGCGACGAGCTCAAGGCGCTGGGCGCCGAACTGCGCGCGCGCCACTGGCACCACCAGGCCGGGCTCG ACCTCGTGCCGGTCGGCGATTTCGCGTTCTACGACCAGGTACTCGACATGAGCTTCACGCTCGGCAACCTGCCCGAGCGG GTGCGCGACTTCCACGGCGATGCGCTCGACAACAGCTTCCGCGTGGCGCGCGGCCGGTCGGCGGCCCCTCATGCCGCGTG CTGCGGCGGGGTCGCGGCCGGCGAGATGACGAAGTGGTTCGACACCAACTACCACTACATCGTCCCCGAATTCACGGCCG ATACCTCATTCACGCTCGATGCCTCGCGCCTGACGGCGCAACTCGCCGAGGCGCGCGCCCAGGGCACGAAGGCCAAGGCC GTGATCATCGGCCCGGTCACCTACCTGTGGCTCGGCAAGACCAAGGACGCGTCGGACAGGCTCGCGCTGCTGCCGCGCCT CCTGCCCGCCTACGCGGCACTGCTCGACGCGCTGGCCGCCGACGGCGTGGCGTGGGTGCAGATCGACGAGCCGGCGCTCG TCACCGAGCTCGACGACGCGTGGCGGCAGGCGTTCATCACGGCCTACGAGGCGCTGAAGTCGCGCAGGGTCAAGCTGCTG CTGGCCACCTACTTCGGCCCGCTGCTCGACAATCTCGATCTGGCCGTGAAGCTGCCCGTCGACGGCCTGCATCTCGACGC GATCCATGCCCGCAGCGAAGTGCTGCTGGCGGCGGACCGGCTGCCGGCCGCGAGCGTGCTGTCGGTGGGCGTCATCAACG GCCGCAACGTCTGGAAGACCGATCTGACGGCCGCGCTCGACTGGCTCGAGCCGCTCGCCGGCCGCCTCGGCGAGCGCCTC TGGATCGCGCCGTCGTGCTCGCTGCTGCACGTGCCGGTCGATCTCGCCGGCGAGACGAAGCTCGACGCCGAGATCCGGTC GTGGCTCGCCTTCGCGCTGCAGAAGCTCGACGAACTGCAGGTGCTGGCCGCCGCGCTGAACCAGGGCCGCGCCGCCGTGG CCGACGCGCTGGCCGCCAACGCCGCCGCCGTCGCGGCGCGCCGCGCGTCGCCGCGCGTGCACAACCCGGCCGTGAAGGCC GCCGTCGCGAAGCTCGACGCGCGGCTCGGCCGGCGCGCGAGCGCCTATGCCGAGCGCGCCGCGAAGCAGGCCGCGCGCCT GCGGCTGCCGAGGTTCCCCACCACCACGATCGGCTCGTTTCCGCAGACGGCCGAGATCCGCCAGGCGCGCAGCCGGTTCA AGGCCGGCACGCTCGACCTGGACGGCTACCGCGCCGCGATGCGCGCCGAGATCGCGCGCAGCGTGCGCGAGCAGGAACGC CTCGGGCTCGACGTGCTGGTCCACGGCGAAGCGGAGCGCAACGACATGGTCGAATACTTCGGCGAGCAGCTCGACGGCTA CGCGTTCAGCCAGTTCGGCTGGGTGCAGTCCTACGGCTCGCGCTGCGTGAAGCCGCCGATCCTGTTCGGCGACATCAGCC GCCCGAAGGCGATGACGGTCGAGTGGATCGCCTATGCGCAGTCGCTGACGCCAAAGCCGATGAAGGGCATGCTGACCGGC CCCGTCACGCTGCTGAACTGGTCGTTCGTGCGTGACGACCAGCCGCGCTCGGTGTCGTGCCGCCAGCTCGCGCTGGCGAT CCGCGAGGAAGTGATCGATCTCGAGAAGGCCGGCGTGAGCGTGATCCAGATCGACGAGGCCGCGCTGCGCGAAGGCCTGC CGCTGCGTCGCGCGCAGTGGGCCGACTACCTGGGCTGGGCAGTCGACGCGTTCCGCATCAGTGCCAACGGCGTGCGCGAC GAGACGCAGATCCACACCCACATGTGCTATTCGGAGTTCAACGACATCATCGCGTCGATCGCCGAGATGGACGCCGACGT CATCACGATCGAGACATCGCGCTCCGACATGGAGCTGCTCGACGCGTTCGACGACTTCAAGTACCCGAACGAGATCGGGC CGGGCGTGTACGACATCCATTCGCCGAACATCCCGACCGAGCAGCAGATCGTCGCGTTGATGAAGAAGGCGGCCGAGCGC GTGCCCGCCGAGCGCCTGTGGGTCAACCCGGACTGCGGCCTGAAGACCCGCCAATGGGCCGAGGTGATTCCGGCGCTGAC CAACATGGTGTCGGCGGCGAAGGCGCTGCGCAGCCAGAGTCGGTAG
Upstream 100 bases:
>100_bases TTCTAATCGATCAATGAAAACAATCCATTTTTCTTCATGAGCGGCGGCCCCTATCATCGCTGCCAGCCCTCCCCTTTCTC CTTGACGACGACGAACCAAC
Downstream 100 bases:
>100_bases GGCGCGATGTCGTCCACTACCGTGTACACTCGGCCCGATTCAATGGATAGCCCGGGGAGGCTGCGATGGAGATCCGCGAC GCGCGCGAGCAGGATGTGGC
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 761; Mature: 761
Protein sequence:
>761_residues MVTTHNLGFPRIGAKRELKFALERYWKGESSRDELKALGAELRARHWHHQAGLDLVPVGDFAFYDQVLDMSFTLGNLPER VRDFHGDALDNSFRVARGRSAAPHAACCGGVAAGEMTKWFDTNYHYIVPEFTADTSFTLDASRLTAQLAEARAQGTKAKA VIIGPVTYLWLGKTKDASDRLALLPRLLPAYAALLDALAADGVAWVQIDEPALVTELDDAWRQAFITAYEALKSRRVKLL LATYFGPLLDNLDLAVKLPVDGLHLDAIHARSEVLLAADRLPAASVLSVGVINGRNVWKTDLTAALDWLEPLAGRLGERL WIAPSCSLLHVPVDLAGETKLDAEIRSWLAFALQKLDELQVLAAALNQGRAAVADALAANAAAVAARRASPRVHNPAVKA AVAKLDARLGRRASAYAERAAKQAARLRLPRFPTTTIGSFPQTAEIRQARSRFKAGTLDLDGYRAAMRAEIARSVREQER LGLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTPKPMKGMLTG PVTLLNWSFVRDDQPRSVSCRQLALAIREEVIDLEKAGVSVIQIDEAALREGLPLRRAQWADYLGWAVDAFRISANGVRD ETQIHTHMCYSEFNDIIASIAEMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPTEQQIVALMKKAAER VPAERLWVNPDCGLKTRQWAEVIPALTNMVSAAKALRSQSR
Sequences:
>Translated_761_residues MVTTHNLGFPRIGAKRELKFALERYWKGESSRDELKALGAELRARHWHHQAGLDLVPVGDFAFYDQVLDMSFTLGNLPER VRDFHGDALDNSFRVARGRSAAPHAACCGGVAAGEMTKWFDTNYHYIVPEFTADTSFTLDASRLTAQLAEARAQGTKAKA VIIGPVTYLWLGKTKDASDRLALLPRLLPAYAALLDALAADGVAWVQIDEPALVTELDDAWRQAFITAYEALKSRRVKLL LATYFGPLLDNLDLAVKLPVDGLHLDAIHARSEVLLAADRLPAASVLSVGVINGRNVWKTDLTAALDWLEPLAGRLGERL WIAPSCSLLHVPVDLAGETKLDAEIRSWLAFALQKLDELQVLAAALNQGRAAVADALAANAAAVAARRASPRVHNPAVKA AVAKLDARLGRRASAYAERAAKQAARLRLPRFPTTTIGSFPQTAEIRQARSRFKAGTLDLDGYRAAMRAEIARSVREQER LGLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTPKPMKGMLTG PVTLLNWSFVRDDQPRSVSCRQLALAIREEVIDLEKAGVSVIQIDEAALREGLPLRRAQWADYLGWAVDAFRISANGVRD ETQIHTHMCYSEFNDIIASIAEMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPTEQQIVALMKKAAER VPAERLWVNPDCGLKTRQWAEVIPALTNMVSAAKALRSQSR >Mature_761_residues MVTTHNLGFPRIGAKRELKFALERYWKGESSRDELKALGAELRARHWHHQAGLDLVPVGDFAFYDQVLDMSFTLGNLPER VRDFHGDALDNSFRVARGRSAAPHAACCGGVAAGEMTKWFDTNYHYIVPEFTADTSFTLDASRLTAQLAEARAQGTKAKA VIIGPVTYLWLGKTKDASDRLALLPRLLPAYAALLDALAADGVAWVQIDEPALVTELDDAWRQAFITAYEALKSRRVKLL LATYFGPLLDNLDLAVKLPVDGLHLDAIHARSEVLLAADRLPAASVLSVGVINGRNVWKTDLTAALDWLEPLAGRLGERL WIAPSCSLLHVPVDLAGETKLDAEIRSWLAFALQKLDELQVLAAALNQGRAAVADALAANAAAVAARRASPRVHNPAVKA AVAKLDARLGRRASAYAERAAKQAARLRLPRFPTTTIGSFPQTAEIRQARSRFKAGTLDLDGYRAAMRAEIARSVREQER LGLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTPKPMKGMLTG PVTLLNWSFVRDDQPRSVSCRQLALAIREEVIDLEKAGVSVIQIDEAALREGLPLRRAQWADYLGWAVDAFRISANGVRD ETQIHTHMCYSEFNDIIASIAEMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPTEQQIVALMKKAAER VPAERLWVNPDCGLKTRQWAEVIPALTNMVSAAKALRSQSR
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=59.6052631578947, Blast_Score=898, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=772, Percent_Identity=47.1502590673575, Blast_Score=693, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 83917; Mature: 83917
Theoretical pI: Translated: 6.80; Mature: 6.80
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVTTHNLGFPRIGAKRELKFALERYWKGESSRDELKALGAELRARHWHHQAGLDLVPVGD CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC FAFYDQVLDMSFTLGNLPERVRDFHGDALDNSFRVARGRSAAPHAACCGGVAAGEMTKWF HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHCCCHHCCHHHHC DTNYHYIVPEFTADTSFTLDASRLTAQLAEARAQGTKAKAVIIGPVTYLWLGKTKDASDR CCCCEEEEEEECCCCCEEECHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCHH LALLPRLLPAYAALLDALAADGVAWVQIDEPALVTELDDAWRQAFITAYEALKSRRVKLL HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LATYFGPLLDNLDLAVKLPVDGLHLDAIHARSEVLLAADRLPAASVLSVGVINGRNVWKT HHHHHHHHHCCCCEEEECCCCCEEEHHHHHCCHHEEECCCCCHHHHHHEEEECCCCCCHH DLTAALDWLEPLAGRLGERLWIAPSCSLLHVPVDLAGETKLDAEIRSWLAFALQKLDELQ HHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH VLAAALNQGRAAVADALAANAAAVAARRASPRVHNPAVKAAVAKLDARLGRRASAYAERA HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH AKQAARLRLPRFPTTTIGSFPQTAEIRQARSRFKAGTLDLDGYRAAMRAEIARSVREQER HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH LGLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTV CCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH EWIAYAQSLTPKPMKGMLTGPVTLLNWSFVRDDQPRSVSCRQLALAIREEVIDLEKAGVS HHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCE VIQIDEAALREGLPLRRAQWADYLGWAVDAFRISANGVRDETQIHTHMCYSEFNDIIASI EEEECHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH AEMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPTEQQIVALMKKAAER HHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH VPAERLWVNPDCGLKTRQWAEVIPALTNMVSAAKALRSQSR CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MVTTHNLGFPRIGAKRELKFALERYWKGESSRDELKALGAELRARHWHHQAGLDLVPVGD CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC FAFYDQVLDMSFTLGNLPERVRDFHGDALDNSFRVARGRSAAPHAACCGGVAAGEMTKWF HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHCCCHHCCHHHHC DTNYHYIVPEFTADTSFTLDASRLTAQLAEARAQGTKAKAVIIGPVTYLWLGKTKDASDR CCCCEEEEEEECCCCCEEECHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCHH LALLPRLLPAYAALLDALAADGVAWVQIDEPALVTELDDAWRQAFITAYEALKSRRVKLL HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LATYFGPLLDNLDLAVKLPVDGLHLDAIHARSEVLLAADRLPAASVLSVGVINGRNVWKT HHHHHHHHHCCCCEEEECCCCCEEEHHHHHCCHHEEECCCCCHHHHHHEEEECCCCCCHH DLTAALDWLEPLAGRLGERLWIAPSCSLLHVPVDLAGETKLDAEIRSWLAFALQKLDELQ HHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH VLAAALNQGRAAVADALAANAAAVAARRASPRVHNPAVKAAVAKLDARLGRRASAYAERA HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH AKQAARLRLPRFPTTTIGSFPQTAEIRQARSRFKAGTLDLDGYRAAMRAEIARSVREQER HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH LGLDVLVHGEAERNDMVEYFGEQLDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTV CCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH EWIAYAQSLTPKPMKGMLTGPVTLLNWSFVRDDQPRSVSCRQLALAIREEVIDLEKAGVS HHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCE VIQIDEAALREGLPLRRAQWADYLGWAVDAFRISANGVRDETQIHTHMCYSEFNDIIASI EEEECHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHH AEMDADVITIETSRSDMELLDAFDDFKYPNEIGPGVYDIHSPNIPTEQQIVALMKKAAER HHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH VPAERLWVNPDCGLKTRQWAEVIPALTNMVSAAKALRSQSR CCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA