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The map label for this gene is dnaK [H]

Identifier: 238023764

GI number: 238023764

Start: 344896

End: 346743

Strand: Reverse

Name: dnaK [H]

Synonym: bglu_2g02960

Alternate gene names: 238023764

Gene position: 346743-344896 (Counterclockwise)

Preceding gene: 238023765

Following gene: 238023763

Centisome position: 12.26

GC content: 73.54

Gene sequence:

>1848_bases
GTGAGCGAGCCGCGTTTCTCGATCGGTATCGATCTCGGTACCACGCATTGCGCGCTGTCCTATATCGATCTCGCCGCAAG
CGACGGCGACGCGCCGCAGCAGCAGGTGCTGCCGATCGCGCAATTGACCTCGCCCGGCGCGATCGGCGCGCCGAGCCTGC
TGCCGTCGTTCCTCTACCTGCCCCATCCGGGCGAACTCACGCCGGGCGACCTGACGCTGCCGTGGACGGCGACGCGCGAC
TACGCGGTGGGCGAGATGGCGCGCAGCCGCGGCGCCGGCACGCCGATCCGGCTCGTGTCGAGCGCGAAGAGCTGGCTCTG
CCATCCGGGCGTCGACCGCCGCGCGGCGATCCTGCCCAACGACGCGCCGCCCGAAGTCGAGCGCGTCTCGCCGCTCGAGG
CATCGGTGCGCTATCTCGCGCATCTGCGCGAGGCCTGGGACCACGCGCACCCGGACGCGCCGTTCGCCGCGCAGGCGGTG
ACGGTCACGATCCCGGCCTCGTTCGATCCGGCCGCGCGCGAGCTGACCGCCGAGGCCGCGCAGGCCGCCGGCTACGCAAG
CACCACGCTGCTGGAGGAGCCGCAGGCCGCGCTCTACAGCTGGATCCAGAAGAGCGGCGGCGGCTGGCGCAAGGACGTGA
AGGTCGGTGACGTCATCCTGGTGGTGGACGTGGGCGGCGGCACCACCGACCTCTCGCTGATCGCCGTGGTCGAGCGCGAC
GGCAAGCTCGAGCTGCACCGCGTGGCGGTGGGCGAGCACATCCTGCTCGGCGGCGACAACATGGACCTCGCGCTCGCGCA
CGTGGTCGCGCGCAAGCTGGCCGCGCAGGGCACCCAGGCCGATCCGTGGCAGCTGCGCGCGCTGACCTACGCGTGCCGCG
GCGCGAAGGAGGCGCTGCTCGGCGACCGCACGCTCGACGCCGTGCCGCTGGTGGTGCCGAGCCGCGGCTCGAAGCTGATC
GGCGGCTCGATCCGCACCGAGCTCACGCGCGCCGAGCTCACGCAGATCCTGCTCGACGGCTTCTTCCCGCAGGTGGACGC
CGCCGCGCGGCCGGCGGTGCGCACGCGCGCCGGCCTCACCCAGCTCGGTCTGCCCTATGCGCAGGACGCCGGCATCACGC
GCCACCTCGCCGCCTTCCTCGGCAAGCAGGTGGGCGCGCTCGCCGAGCTCGGCGGGCAGCCGGCTGCACCGGCCGGCGGC
GCGAGCTTCCTGCACCCCACCGCCGTGCTGTTCAACGGCGGCGTGTTCAAGTCCGACCTGCTGACCGAGCGCGTCCTCAC
GATCCTGAACGGCTGGCTGGCGGCCGACGGGGCCGCGCCCGCGCGCCTGCTCGGCGGCGCCGACCTCGACCTCGCGGTGG
CGCGCGGCGCGGCCTACTACGGCTACGTCAAGCGCGGCCGCGGCGTGCGGATTCGCGGCGGCACGGCACGCGCCTACTAC
ATCGCCATCGAATCGGCGATGCCGGCGATCCCCGGCATGGAGCCGCCCGTCTGCGCGCTCTGCGTGGCGCCGTTCGGCAT
GGAGGAAGGCTCGGACGCCGCGCTGCCCGAGCAGGAGTTCGGCCTGGTGGTGGGCGAACCGGTGACGTTCCGCTTCTTCG
GCTCGTCGGTGCGGCGCCAGGATCAGGTCGGCACCCTGCTCGACTACTGGTCCGCCGACGAGCTGCAGGAACTCGAGGCG
ATCCAGGCCACGCTGCCCGCCGAGGGCCGCACGCCCGGCGAAGTGGTGGCCGTGCGGCTGCACGCACGCGTGACCGAAGC
CGGCACCCTCGAACTCGAAGCGATGCCGCGTGGCAGCGGCGAGCGCTGGAAGGTCGAATTCGACGTGCGCGGCGGCGCGG
ACGCCTGA

Upstream 100 bases:

>100_bases
GCACGCTGAGCCACCGCGGCTGGCGCGCCGCCGAGGTGAAGCTGCCGCAGCGGGTCGGCACCCACGACCCGAGCGTGATC
GCGCCGGCGGAGGTGGAGCT

Downstream 100 bases:

>100_bases
TGGCGCGAGCGAAGGCACGCTACGTCGTCGGCATCGACCTCGGCACCAGCAACACCGTGGTCGCCTACGTCGAGGCGGGC
GGCACCGCGATCCGCCTGTT

Product: putative DnaK-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSEPRFSIGIDLGTTHCALSYIDLAASDGDAPQQQVLPIAQLTSPGAIGAPSLLPSFLYLPHPGELTPGDLTLPWTATRD
YAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVERVSPLEASVRYLAHLREAWDHAHPDAPFAAQAV
TVTIPASFDPAARELTAEAAQAAGYASTTLLEEPQAALYSWIQKSGGGWRKDVKVGDVILVVDVGGGTTDLSLIAVVERD
GKLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKEALLGDRTLDAVPLVVPSRGSKLI
GGSIRTELTRAELTQILLDGFFPQVDAAARPAVRTRAGLTQLGLPYAQDAGITRHLAAFLGKQVGALAELGGQPAAPAGG
ASFLHPTAVLFNGGVFKSDLLTERVLTILNGWLAADGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
IAIESAMPAIPGMEPPVCALCVAPFGMEEGSDAALPEQEFGLVVGEPVTFRFFGSSVRRQDQVGTLLDYWSADELQELEA
IQATLPAEGRTPGEVVAVRLHARVTEAGTLELEAMPRGSGERWKVEFDVRGGADA

Sequences:

>Translated_615_residues
MSEPRFSIGIDLGTTHCALSYIDLAASDGDAPQQQVLPIAQLTSPGAIGAPSLLPSFLYLPHPGELTPGDLTLPWTATRD
YAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVERVSPLEASVRYLAHLREAWDHAHPDAPFAAQAV
TVTIPASFDPAARELTAEAAQAAGYASTTLLEEPQAALYSWIQKSGGGWRKDVKVGDVILVVDVGGGTTDLSLIAVVERD
GKLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKEALLGDRTLDAVPLVVPSRGSKLI
GGSIRTELTRAELTQILLDGFFPQVDAAARPAVRTRAGLTQLGLPYAQDAGITRHLAAFLGKQVGALAELGGQPAAPAGG
ASFLHPTAVLFNGGVFKSDLLTERVLTILNGWLAADGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
IAIESAMPAIPGMEPPVCALCVAPFGMEEGSDAALPEQEFGLVVGEPVTFRFFGSSVRRQDQVGTLLDYWSADELQELEA
IQATLPAEGRTPGEVVAVRLHARVTEAGTLELEAMPRGSGERWKVEFDVRGGADA
>Mature_614_residues
SEPRFSIGIDLGTTHCALSYIDLAASDGDAPQQQVLPIAQLTSPGAIGAPSLLPSFLYLPHPGELTPGDLTLPWTATRDY
AVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVERVSPLEASVRYLAHLREAWDHAHPDAPFAAQAVT
VTIPASFDPAARELTAEAAQAAGYASTTLLEEPQAALYSWIQKSGGGWRKDVKVGDVILVVDVGGGTTDLSLIAVVERDG
KLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKEALLGDRTLDAVPLVVPSRGSKLIG
GSIRTELTRAELTQILLDGFFPQVDAAARPAVRTRAGLTQLGLPYAQDAGITRHLAAFLGKQVGALAELGGQPAAPAGGA
SFLHPTAVLFNGGVFKSDLLTERVLTILNGWLAADGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYYI
AIESAMPAIPGMEPPVCALCVAPFGMEEGSDAALPEQEFGLVVGEPVTFRFFGSSVRRQDQVGTLLDYWSADELQELEAI
QATLPAEGRTPGEVVAVRLHARVTEAGTLELEAMPRGSGERWKVEFDVRGGADA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=354, Percent_Identity=25.9887005649718, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788875, Length=307, Percent_Identity=28.3387622149837, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17534015, Length=250, Percent_Identity=26.4, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17534013, Length=250, Percent_Identity=26.4, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17541098, Length=189, Percent_Identity=30.1587301587302, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 64808; Mature: 64677

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRFSIGIDLGTTHCALSYIDLAASDGDAPQQQVLPIAQLTSPGAIGAPSLLPSFLYL
CCCCCEEEEEECCCHHHHHHHHEEECCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHHEEC
PHPGELTPGDLTLPWTATRDYAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPN
CCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVERVSPLEASVRYLAHLREAWDHAHPDAPFAAQAVTVTIPASFDPAARELTAEAA
CCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
QAAGYASTTLLEEPQAALYSWIQKSGGGWRKDVKVGDVILVVDVGGGTTDLSLIAVVERD
HHCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCEECCEEEEEECCCCCCCEEEEEEEECC
GKLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKEALL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
GDRTLDAVPLVVPSRGSKLIGGSIRTELTRAELTQILLDGFFPQVDAAARPAVRTRAGLT
CCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCHH
QLGLPYAQDAGITRHLAAFLGKQVGALAELGGQPAAPAGGASFLHPTAVLFNGGVFKSDL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHEECCCCCHHHH
LTERVLTILNGWLAADGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCCEEEE
IAIESAMPAIPGMEPPVCALCVAPFGMEEGSDAALPEQEFGLVVGEPVTFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHHH
DQVGTLLDYWSADELQELEAIQATLPAEGRTPGEVVAVRLHARVTEAGTLELEAMPRGSG
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCC
ERWKVEFDVRGGADA
CEEEEEEEECCCCCC
>Mature Secondary Structure 
SEPRFSIGIDLGTTHCALSYIDLAASDGDAPQQQVLPIAQLTSPGAIGAPSLLPSFLYL
CCCCEEEEEECCCHHHHHHHHEEECCCCCCCHHHCCCHHHHCCCCCCCCHHHHHHHEEC
PHPGELTPGDLTLPWTATRDYAVGEMARSRGAGTPIRLVSSAKSWLCHPGVDRRAAILPN
CCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEECCC
DAPPEVERVSPLEASVRYLAHLREAWDHAHPDAPFAAQAVTVTIPASFDPAARELTAEAA
CCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
QAAGYASTTLLEEPQAALYSWIQKSGGGWRKDVKVGDVILVVDVGGGTTDLSLIAVVERD
HHCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCEECCEEEEEECCCCCCCEEEEEEEECC
GKLELHRVAVGEHILLGGDNMDLALAHVVARKLAAQGTQADPWQLRALTYACRGAKEALL
CCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
GDRTLDAVPLVVPSRGSKLIGGSIRTELTRAELTQILLDGFFPQVDAAARPAVRTRAGLT
CCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHCCHH
QLGLPYAQDAGITRHLAAFLGKQVGALAELGGQPAAPAGGASFLHPTAVLFNGGVFKSDL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHEECCCCCHHHH
LTERVLTILNGWLAADGAAPARLLGGADLDLAVARGAAYYGYVKRGRGVRIRGGTARAYY
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCCEEEE
IAIESAMPAIPGMEPPVCALCVAPFGMEEGSDAALPEQEFGLVVGEPVTFRFFGSSVRRQ
EEEECCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCHHHCCEEECCCEEEEECCCHHHHH
DQVGTLLDYWSADELQELEAIQATLPAEGRTPGEVVAVRLHARVTEAGTLELEAMPRGSG
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCC
ERWKVEFDVRGGADA
CEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]