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The map label for this gene is ynfL [H]

Identifier: 238023677

GI number: 238023677

Start: 229467

End: 230375

Strand: Direct

Name: ynfL [H]

Synonym: bglu_2g01990

Alternate gene names: 238023677

Gene position: 229467-230375 (Clockwise)

Preceding gene: 238023675

Following gene: 238023678

Centisome position: 8.12

GC content: 69.64

Gene sequence:

>909_bases
GTGGAACTGAGACAACTACGCCATTTCGTCGCCGTCGCCGAGGAGGCGAACTTCACGCGGGCCGCCGAGCGCTGCCACAT
CGTGCAGTCCGCGCTGTCCACCTCGATCCGCCTGCTGGAGGAGGAACTCGGCGCGAGGCTGCTGGTGCGCACCACACGCC
GCGTGAGCCTGTCGGCAGCCGGCGCGGTGTTTCTCGACAGCGCGCGGCGCGCGCTCGACATCCTCGATCGGGCAGGGCTG
GAGGTGGCCGACATCACCTCGGTGCGGCGCGGCAAGCTGTCGATCGGCACGGTGCAGAGCCTGCCGCAATTTCTCGAATT
GCCGGCGCTGCTGTCGCGTTTCTACCACGCTCATCCGGGCGTGGAGGTGCGGCTCGTGCAGGGCGGCGCGGCCGAGCTGA
ACGAAAAGGTCGACGCGCGTGAACTCGATTTGGCGATCCTGCCGATCGAGGAGCGCAACGAGCGGCTCGCATCGCACGTG
ATCGCCTGCGACGAGATGGTGCTCGCCTGCAGGCGTGATCATCCGCTCGCCGCGTCGGGGCAGGTGCCGCTGTCGCGGCT
CACGAAGCAGGCGTTCGTCGATTTCGTGCCGGGGCAGGGCACGCGCCGGTTGGTAGACCGTGGCTTCGCGGAGGCTGGGC
TCCAGCGGCGCGTGGCCTTCGAGATCGGCGATCTCGACACACTGCTCGAACTGGTCCGCCAGGGGCTCGGTGTTGCGCTG
CTGCCCGAGGACATCGTGGTGCGCCGCCCCGACATGCTCGCCTGCGTGCGGCTCGAACAGACCCAGCTGTGTTGGGAGCT
GGTGGTCACGCACGCGGCCTCTGCGGCCGGGGACGCGCCGGACCCGCTCGACCCGGCGCCCGTCGCGTTTCTCGACATGC
TGATGGCGGAGACGGCCGGCCAGCGCTGA

Upstream 100 bases:

>100_bases
TGCCCGGTCGCGTCGGAGATGAGACCGGCGCCGCGCAAGCCGGGCGGGCAAGGCAACGACGAGGCCGTGGCATGCCACGA
GGAATGACCAGAGGATTGCA

Downstream 100 bases:

>100_bases
GCCGCGGATGGCGCGGCGGCGTGGCCGGCCCGTGCCGGCCGCCGGGACGGACCCGACGGCATTCTGTCGTAAGAATGGCG
TAATTTCGTCAAGCGCAGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MELRQLRHFVAVAEEANFTRAAERCHIVQSALSTSIRLLEEELGARLLVRTTRRVSLSAAGAVFLDSARRALDILDRAGL
EVADITSVRRGKLSIGTVQSLPQFLELPALLSRFYHAHPGVEVRLVQGGAAELNEKVDARELDLAILPIEERNERLASHV
IACDEMVLACRRDHPLAASGQVPLSRLTKQAFVDFVPGQGTRRLVDRGFAEAGLQRRVAFEIGDLDTLLELVRQGLGVAL
LPEDIVVRRPDMLACVRLEQTQLCWELVVTHAASAAGDAPDPLDPAPVAFLDMLMAETAGQR

Sequences:

>Translated_302_residues
MELRQLRHFVAVAEEANFTRAAERCHIVQSALSTSIRLLEEELGARLLVRTTRRVSLSAAGAVFLDSARRALDILDRAGL
EVADITSVRRGKLSIGTVQSLPQFLELPALLSRFYHAHPGVEVRLVQGGAAELNEKVDARELDLAILPIEERNERLASHV
IACDEMVLACRRDHPLAASGQVPLSRLTKQAFVDFVPGQGTRRLVDRGFAEAGLQRRVAFEIGDLDTLLELVRQGLGVAL
LPEDIVVRRPDMLACVRLEQTQLCWELVVTHAASAAGDAPDPLDPAPVAFLDMLMAETAGQR
>Mature_302_residues
MELRQLRHFVAVAEEANFTRAAERCHIVQSALSTSIRLLEEELGARLLVRTTRRVSLSAAGAVFLDSARRALDILDRAGL
EVADITSVRRGKLSIGTVQSLPQFLELPALLSRFYHAHPGVEVRLVQGGAAELNEKVDARELDLAILPIEERNERLASHV
IACDEMVLACRRDHPLAASGQVPLSRLTKQAFVDFVPGQGTRRLVDRGFAEAGLQRRVAFEIGDLDTLLELVRQGLGVAL
LPEDIVVRRPDMLACVRLEQTQLCWELVVTHAASAAGDAPDPLDPAPVAFLDMLMAETAGQR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=37.5, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI145693105, Length=241, Percent_Identity=31.1203319502075, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI157672245, Length=255, Percent_Identity=32.156862745098, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=32.2448979591837, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=35.2459016393443, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=31.25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=30.6451612903226, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI2367136, Length=241, Percent_Identity=28.6307053941909, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787530, Length=266, Percent_Identity=27.8195488721804, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=31.640625, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=29.6153846153846, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=29.9435028248588, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788481, Length=246, Percent_Identity=26.0162601626016, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=244, Percent_Identity=25.4098360655738, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=125, Percent_Identity=37.6, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=27.9352226720648, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994962, Length=192, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=30.1587301587302, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081904, Length=208, Percent_Identity=26.4423076923077, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=173, Percent_Identity=28.9017341040462, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33097; Mature: 33097

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRHFVAVAEEANFTRAAERCHIVQSALSTSIRLLEEELGARLLVRTTRRVSLSAA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GAVFLDSARRALDILDRAGLEVADITSVRRGKLSIGTVQSLPQFLELPALLSRFYHAHPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VEVRLVQGGAAELNEKVDARELDLAILPIEERNERLASHVIACDEMVLACRRDHPLAASG
CEEEEECCCHHHHHHCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QVPLSRLTKQAFVDFVPGQGTRRLVDRGFAEAGLQRRVAFEIGDLDTLLELVRQGLGVAL
CCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
LPEDIVVRRPDMLACVRLEQTQLCWELVVTHAASAAGDAPDPLDPAPVAFLDMLMAETAG
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
QR
CC
>Mature Secondary Structure
MELRQLRHFVAVAEEANFTRAAERCHIVQSALSTSIRLLEEELGARLLVRTTRRVSLSAA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GAVFLDSARRALDILDRAGLEVADITSVRRGKLSIGTVQSLPQFLELPALLSRFYHAHPG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VEVRLVQGGAAELNEKVDARELDLAILPIEERNERLASHVIACDEMVLACRRDHPLAASG
CEEEEECCCHHHHHHCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QVPLSRLTKQAFVDFVPGQGTRRLVDRGFAEAGLQRRVAFEIGDLDTLLELVRQGLGVAL
CCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
LPEDIVVRRPDMLACVRLEQTQLCWELVVTHAASAAGDAPDPLDPAPVAFLDMLMAETAG
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
QR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]