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The map label for this gene is oprM [H]

Identifier: 238023621

GI number: 238023621

Start: 140129

End: 141715

Strand: Direct

Name: oprM [H]

Synonym: bglu_2g01260

Alternate gene names: 238023621

Gene position: 140129-141715 (Clockwise)

Preceding gene: 238023620

Following gene: 238023622

Centisome position: 4.96

GC content: 68.87

Gene sequence:

>1587_bases
ATGAAGGTGCAATTCGTAACCGGACGCAACGGGGGCAGCAAGCACCGACTTTGCAGGCTGGCCATCGCGGCGGCCGTGCT
ATGCACCACGACGGGCTGCACGCTGGCACCGCATTACACGCGGCCCGACGCGCCCGTTGCGCAGGCGTACCCGGCCGACG
GCGTCTACGCTGCGCAGCCGGGCGCGGCCGGCACGCGCAGCGCGAACGGCCAGCCGGCCACCGCGATCGGCTGGCGCGAA
TTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATCGCGCTGAAGAACAACCGCGATCTGCGCGTCGCGGTGCTGAA
CATCGAGGCATCGCGGGCGCAGTACCAGATCACGCGTGCGGGGCTGTTGCCGACACTCGAGGCGACCGGAACCGGCAATC
GCCAGCGTGTGCCGAGTTCGCTCGCGGCGATTCCGGGCCGCAACCTCACGACCACCTACAACGTCGGCCTGTCCGCGTCG
TGGGAACTCGACCTGTTCGGCCGGGTCCAGAGCCTGAAGGACCAGGCCCTCGCGCAGTACCTGGCGACGTCTTACGCGCG
GCAGGCGACCGAGATCTCGCTCGTGTCGCAGGTGGCCGACCAGTACCTGACGCTGCTGTCGACCGACGAGCTGCTGAAGG
TGACGGAAAACACGCTGAAGAACGCGCAGGCGTCTTACGACCTGACGAAGCTGCAGTTCGACAACGGCACGGGCTCGGAG
CTCGACTTGCGTCAGGCCCAGACGGTGGTCGAGCAGGCGCTTGCTAACCAGCAGGCGCAGGCGCGTGCCCGCGCGCAGGC
ACTGAACGCGCTGGTGCTGCTGATCGGCGAGCCGCTGCCGGACGACCTGCCGGCGGGCATGCCGCTCGACGCGCAGAACC
TGCTGACCGACGTGCCGGCCGGATTGCCGTCGGATCTGCTCACGCGCCGTCCGGACGTGATGCAGGCCGAGCAGATGCTG
AAGGCAGCGAACGCGAACATCGGCGCGGCACGCGCTGCGTTCTTCCCGCGCATCTCGCTGACGGGCGCGTTCGGCACCGC
CAGCCCGACGCTCGACGGCCTGTTCAAGGCCGGCACGGCGGCCTGGTCGTTCGCGCCGCAACTCACCCTGCCGATCTTCG
CGGGCGGGCAGAACATCGCGAACCTGGATCTCGCCAACGTGCAGAAGCGCATCGAGATCTCGAACTACGAGAAGGCGATC
CAGTCGGCATTCCGCGAAGTATCGGACGGCCTCGCCGCACGCGGCACATACGACCAGGAGATTGCAGCACTCGAGCGTAA
CGAGCACGCGCAGCAGCGTCGCTTCGAGCTGTCGAACCTGCGCTACAGGAACGGGGTGGACAGCTACCTGTCGGTGCTGA
CCGCGCAGACGGACCTGTACTCGGCGCGGCAGTCGCTGATCAATGCGCGTCTGGCCCGCTGGACGAATCTCGTCGCGCTG
TATCGCGCGCTCGGCGGCGGCTGGATCCAGCGCGCGGGCGACACGCCGCGCGCGCCCGATGCCCCGGCCGATCGAGACAC
GGCGGCCGGCGCGGCGGCGTCGATGGCTGTCGGGGCTCGCCGAGAGGCCGGTTCCAATTCCCCGTGA

Upstream 100 bases:

>100_bases
CGCTGTTTACGCTGTTCGTGCTGCCGACCGTGTATACGCTGATCGCCAAGCGCCATCGCGAGGCGGCGAACAATGAACGC
GCCCGCGTGCTGGAGGCCGT

Downstream 100 bases:

>100_bases
AGGACCGCTTACCTGCTCGGCAATTCCCCGACCTTGGGCCTCGGACCAATGGCGACCAGAGGTGGAGGCGCCGGTTGTTG
ACGACGAGCGGTGGGAGCCG

Product: multidrug efflux system outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKVQFVTGRNGGSKHRLCRLAIAAAVLCTTTGCTLAPHYTRPDAPVAQAYPADGVYAAQPGAAGTRSANGQPATAIGWRE
FFVDPRLQRLIEIALKNNRDLRVAVLNIEASRAQYQITRAGLLPTLEATGTGNRQRVPSSLAAIPGRNLTTTYNVGLSAS
WELDLFGRVQSLKDQALAQYLATSYARQATEISLVSQVADQYLTLLSTDELLKVTENTLKNAQASYDLTKLQFDNGTGSE
LDLRQAQTVVEQALANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQML
KAANANIGAARAAFFPRISLTGAFGTASPTLDGLFKAGTAAWSFAPQLTLPIFAGGQNIANLDLANVQKRIEISNYEKAI
QSAFREVSDGLAARGTYDQEIAALERNEHAQQRRFELSNLRYRNGVDSYLSVLTAQTDLYSARQSLINARLARWTNLVAL
YRALGGGWIQRAGDTPRAPDAPADRDTAAGAAASMAVGARREAGSNSP

Sequences:

>Translated_528_residues
MKVQFVTGRNGGSKHRLCRLAIAAAVLCTTTGCTLAPHYTRPDAPVAQAYPADGVYAAQPGAAGTRSANGQPATAIGWRE
FFVDPRLQRLIEIALKNNRDLRVAVLNIEASRAQYQITRAGLLPTLEATGTGNRQRVPSSLAAIPGRNLTTTYNVGLSAS
WELDLFGRVQSLKDQALAQYLATSYARQATEISLVSQVADQYLTLLSTDELLKVTENTLKNAQASYDLTKLQFDNGTGSE
LDLRQAQTVVEQALANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQML
KAANANIGAARAAFFPRISLTGAFGTASPTLDGLFKAGTAAWSFAPQLTLPIFAGGQNIANLDLANVQKRIEISNYEKAI
QSAFREVSDGLAARGTYDQEIAALERNEHAQQRRFELSNLRYRNGVDSYLSVLTAQTDLYSARQSLINARLARWTNLVAL
YRALGGGWIQRAGDTPRAPDAPADRDTAAGAAASMAVGARREAGSNSP
>Mature_528_residues
MKVQFVTGRNGGSKHRLCRLAIAAAVLCTTTGCTLAPHYTRPDAPVAQAYPADGVYAAQPGAAGTRSANGQPATAIGWRE
FFVDPRLQRLIEIALKNNRDLRVAVLNIEASRAQYQITRAGLLPTLEATGTGNRQRVPSSLAAIPGRNLTTTYNVGLSAS
WELDLFGRVQSLKDQALAQYLATSYARQATEISLVSQVADQYLTLLSTDELLKVTENTLKNAQASYDLTKLQFDNGTGSE
LDLRQAQTVVEQALANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQML
KAANANIGAARAAFFPRISLTGAFGTASPTLDGLFKAGTAAWSFAPQLTLPIFAGGQNIANLDLANVQKRIEISNYEKAI
QSAFREVSDGLAARGTYDQEIAALERNEHAQQRRFELSNLRYRNGVDSYLSVLTAQTDLYSARQSLINARLARWTNLVAL
YRALGGGWIQRAGDTPRAPDAPADRDTAAGAAASMAVGARREAGSNSP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=472, Percent_Identity=41.1016949152542, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI2367348, Length=498, Percent_Identity=27.1084337349398, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56629; Mature: 56629

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVQFVTGRNGGSKHRLCRLAIAAAVLCTTTGCTLAPHYTRPDAPVAQAYPADGVYAAQP
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHCCCCCCEEECCC
GAAGTRSANGQPATAIGWREFFVDPRLQRLIEIALKNNRDLRVAVLNIEASRAQYQITRA
CCCCCCCCCCCCCEEHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEHHH
GLLPTLEATGTGNRQRVPSSLAAIPGRNLTTTYNVGLSASWELDLFGRVQSLKDQALAQY
CCCCCEECCCCCCHHHCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LATSYARQATEISLVSQVADQYLTLLSTDELLKVTENTLKNAQASYDLTKLQFDNGTGSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
LDLRQAQTVVEQALANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCC
GLPSDLLTRRPDVMQAEQMLKAANANIGAARAAFFPRISLTGAFGTASPTLDGLFKAGTA
CCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCH
AWSFAPQLTLPIFAGGQNIANLDLANVQKRIEISNYEKAIQSAFREVSDGLAARGTYDQE
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHH
IAALERNEHAQQRRFELSNLRYRNGVDSYLSVLTAQTDLYSARQSLINARLARWTNLVAL
HHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALGGGWIQRAGDTPRAPDAPADRDTAAGAAASMAVGARREAGSNSP
HHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVQFVTGRNGGSKHRLCRLAIAAAVLCTTTGCTLAPHYTRPDAPVAQAYPADGVYAAQP
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHCCCCCCEEECCC
GAAGTRSANGQPATAIGWREFFVDPRLQRLIEIALKNNRDLRVAVLNIEASRAQYQITRA
CCCCCCCCCCCCCEEHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEHHH
GLLPTLEATGTGNRQRVPSSLAAIPGRNLTTTYNVGLSASWELDLFGRVQSLKDQALAQY
CCCCCEECCCCCCHHHCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LATSYARQATEISLVSQVADQYLTLLSTDELLKVTENTLKNAQASYDLTKLQFDNGTGSE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
LDLRQAQTVVEQALANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCC
GLPSDLLTRRPDVMQAEQMLKAANANIGAARAAFFPRISLTGAFGTASPTLDGLFKAGTA
CCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCH
AWSFAPQLTLPIFAGGQNIANLDLANVQKRIEISNYEKAIQSAFREVSDGLAARGTYDQE
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHH
IAALERNEHAQQRRFELSNLRYRNGVDSYLSVLTAQTDLYSARQSLINARLARWTNLVAL
HHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRALGGGWIQRAGDTPRAPDAPADRDTAAGAAASMAVGARREAGSNSP
HHHHCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]