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The map label for this gene is bcr [H]

Identifier: 238023617

GI number: 238023617

Start: 134165

End: 135355

Strand: Direct

Name: bcr [H]

Synonym: bglu_2g01220

Alternate gene names: 238023617

Gene position: 134165-135355 (Clockwise)

Preceding gene: 238023616

Following gene: 238023618

Centisome position: 4.75

GC content: 66.5

Gene sequence:

>1191_bases
ATGTCGAACTCAAACAGATACGAAAATTTCCTCGTGTTCCTAGCCCCGCTGATCAACAGCGTCGGCGGGATCGCGATCGA
CCTCTATGCGCCCAGCATTCCGGCCATCGGTCGAGAACTCAATGTCATGCCGAGCATGATGCAGAACACCATCACGATCA
CGCTCGTGTTCTATGCGATCGGCCAGCTCGCGTTCGGCATGCTGACGGACTGGTGGGGCAGACGCCCGTCGATACTGTTC
GGCCTTGCGCTGTTCATCGCCGGCAGTGCGCTCGCGGTCGCCGCGCCCTCGTTTGAGGTGCTGATGGCGGCACGCGCGAT
TCAGGGCTTCGCGATCGGATCATGCCAGGTCGTGGCGCGCGCCGTGCTGGTCGACCGGCTCAAGGGCGACCGCTTCCGCG
TCGCGATCGTCTACCTCAGCCTCGCGTTCGGGCTGGGCCCCGTGGTCGCGCCGTATGTGGGCGGACACGTGCAGGAGTGG
GCCGGCTGGCGCTGGAACTTCGTGGTGTACTGCGGCTACGGCCTGGTCGTGCTGTCGTTCGCGTTCGCGGGCCTGCGCGA
GACGCTGCGGCCGGAGCTGCGGCGCACGCCGCGGCAGACCATTGCGGGCTATCGCGAGATCCTGTCCGACTCGCACTTCC
AGTCGGCCGTGGCGATGCTCGGGATGAGCTTCTCGGCGTTCCTGATGTGGAACGTGATCGGGCCGTACATCGTGCAAACC
CGCCTCGGGCATTCCGCGTCGTACTTCGGTGCCACCGCGCTCGGCGTCGGCGTGTGCTACCTCGGCGGCACGACGCTGAA
CCGGAGCCTGATCCGGCGCTTCAACGGCGAGCAGCTGATGCGCGGCGGCATCGTGACCTTTGCGCTCGGGGTCGCCTGCG
TGGCATCGAGCGGCAGCGAGCTGAGCCTGGCGACCGCGCTCGGCGGGATCATGCTGATCGCGTTTGCGCAGGGCTTCATC
TTCTCCAACGCGATGGCTCGCTCGATGGCCCTGTTCCCGGGCCGCGCGGGCGCCGCCGCAAGCCTGCAGGGATGCCTGAT
GCTTGCGCTCGGCTCGATCGTGTCGGGGATCGTCAGCGCGCTGCCGATGGAGACGAACGCGGCGATCGCCATCATGTTCG
CGTGTCTGCTGGGCGTGACCATGATCGGCTTCCGGCAACTGCATCGCCTGCATCCACAGGTGGCGCGATGA

Upstream 100 bases:

>100_bases
TATGCCGCTCAGGCAGGTTGAATACGCGGCAAACGGTCATTGATTCCGGCAGGCCGCACGCACTCGCGTCGGCCTGTCCC
GTCATTTCGGGAGCGTCCTC

Downstream 100 bases:

>100_bases
AGGCCGGCCTCGCGCTCGCGCTGCGGCTGGTACTGGGCGCGTTCTGGAGCTGGGCGGGCTTGACGAAGATCGTCGGGCAC
TTCGATGCGGGGCCCTTCCT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSNSNRYENFLVFLAPLINSVGGIAIDLYAPSIPAIGRELNVMPSMMQNTITITLVFYAIGQLAFGMLTDWWGRRPSILF
GLALFIAGSALAVAAPSFEVLMAARAIQGFAIGSCQVVARAVLVDRLKGDRFRVAIVYLSLAFGLGPVVAPYVGGHVQEW
AGWRWNFVVYCGYGLVVLSFAFAGLRETLRPELRRTPRQTIAGYREILSDSHFQSAVAMLGMSFSAFLMWNVIGPYIVQT
RLGHSASYFGATALGVGVCYLGGTTLNRSLIRRFNGEQLMRGGIVTFALGVACVASSGSELSLATALGGIMLIAFAQGFI
FSNAMARSMALFPGRAGAAASLQGCLMLALGSIVSGIVSALPMETNAAIAIMFACLLGVTMIGFRQLHRLHPQVAR

Sequences:

>Translated_396_residues
MSNSNRYENFLVFLAPLINSVGGIAIDLYAPSIPAIGRELNVMPSMMQNTITITLVFYAIGQLAFGMLTDWWGRRPSILF
GLALFIAGSALAVAAPSFEVLMAARAIQGFAIGSCQVVARAVLVDRLKGDRFRVAIVYLSLAFGLGPVVAPYVGGHVQEW
AGWRWNFVVYCGYGLVVLSFAFAGLRETLRPELRRTPRQTIAGYREILSDSHFQSAVAMLGMSFSAFLMWNVIGPYIVQT
RLGHSASYFGATALGVGVCYLGGTTLNRSLIRRFNGEQLMRGGIVTFALGVACVASSGSELSLATALGGIMLIAFAQGFI
FSNAMARSMALFPGRAGAAASLQGCLMLALGSIVSGIVSALPMETNAAIAIMFACLLGVTMIGFRQLHRLHPQVAR
>Mature_395_residues
SNSNRYENFLVFLAPLINSVGGIAIDLYAPSIPAIGRELNVMPSMMQNTITITLVFYAIGQLAFGMLTDWWGRRPSILFG
LALFIAGSALAVAAPSFEVLMAARAIQGFAIGSCQVVARAVLVDRLKGDRFRVAIVYLSLAFGLGPVVAPYVGGHVQEWA
GWRWNFVVYCGYGLVVLSFAFAGLRETLRPELRRTPRQTIAGYREILSDSHFQSAVAMLGMSFSAFLMWNVIGPYIVQTR
LGHSASYFGATALGVGVCYLGGTTLNRSLIRRFNGEQLMRGGIVTFALGVACVASSGSELSLATALGGIMLIAFAQGFIF
SNAMARSMALFPGRAGAAASLQGCLMLALGSIVSGIVSALPMETNAAIAIMFACLLGVTMIGFRQLHRLHPQVAR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=363, Percent_Identity=26.7217630853994, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994889, Length=373, Percent_Identity=27.0777479892761, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790146, Length=325, Percent_Identity=30.1538461538462, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=204, Percent_Identity=31.3725490196078, Blast_Score=83, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=27.810650887574, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42333; Mature: 42201

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSNRYENFLVFLAPLINSVGGIAIDLYAPSIPAIGRELNVMPSMMQNTITITLVFYAI
CCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQLAFGMLTDWWGRRPSILFGLALFIAGSALAVAAPSFEVLMAARAIQGFAIGSCQVVAR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHCCCCCHHHHHHH
AVLVDRLKGDRFRVAIVYLSLAFGLGPVVAPYVGGHVQEWAGWRWNFVVYCGYGLVVLSF
HHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCEEEEEEECHHHHHHHH
AFAGLRETLRPELRRTPRQTIAGYREILSDSHFQSAVAMLGMSFSAFLMWNVIGPYIVQT
HHHHHHHHHCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGHSASYFGATALGVGVCYLGGTTLNRSLIRRFNGEQLMRGGIVTFALGVACVASSGSE
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCC
LSLATALGGIMLIAFAQGFIFSNAMARSMALFPGRAGAAASLQGCLMLALGSIVSGIVSA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPMETNAAIAIMFACLLGVTMIGFRQLHRLHPQVAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCC
>Mature Secondary Structure 
SNSNRYENFLVFLAPLINSVGGIAIDLYAPSIPAIGRELNVMPSMMQNTITITLVFYAI
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GQLAFGMLTDWWGRRPSILFGLALFIAGSALAVAAPSFEVLMAARAIQGFAIGSCQVVAR
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEECCHHHHHHHHHHHHCCCCCHHHHHHH
AVLVDRLKGDRFRVAIVYLSLAFGLGPVVAPYVGGHVQEWAGWRWNFVVYCGYGLVVLSF
HHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCEEEEEEECHHHHHHHH
AFAGLRETLRPELRRTPRQTIAGYREILSDSHFQSAVAMLGMSFSAFLMWNVIGPYIVQT
HHHHHHHHHCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGHSASYFGATALGVGVCYLGGTTLNRSLIRRFNGEQLMRGGIVTFALGVACVASSGSE
HCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCC
LSLATALGGIMLIAFAQGFIFSNAMARSMALFPGRAGAAASLQGCLMLALGSIVSGIVSA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LPMETNAAIAIMFACLLGVTMIGFRQLHRLHPQVAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]