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The map label for this gene is ndhF [H]

Identifier: 238023609

GI number: 238023609

Start: 123579

End: 125171

Strand: Direct

Name: ndhF [H]

Synonym: bglu_2g01140

Alternate gene names: 238023609

Gene position: 123579-125171 (Clockwise)

Preceding gene: 238023607

Following gene: 238023610

Centisome position: 4.37

GC content: 68.61

Gene sequence:

>1593_bases
ATGATTCCCACCCACCTCGTCTTATTCCTCTGCGCATTGGCGCCGGCGGCGCTCATGTCGACAGCCGCGGGTCGACTGGC
ATGGCGGCCCGATGACGGCCGCGACGTGTGGAAACCCTTCCACCGGCTGGCAGCGGTCGCGTTGTTGATCGTGTTGATCC
AGCCGGTCGTGCTGGCGACGCTTCACGAGCGCGCCGCGGCTCATGAGGGCGGGTGGACCGCCTGGATCGAAGTGACGCCG
CTCGCCGCCTGGATGGCCGCGCTGGTGCAGCTGCTCGGCACCGTGATCGGCGTCTTCTCCGGGCGCTATCTGCAAGGAGA
ACCGGGGCAGCCGCGCTACGCGGCTGCGTTGGCCGGGGTCCTGGCGTCGGTGCACGTGCTCCTGCTGTCCGGCCACTGGC
TGCTCCTGATCGCTGCCTGGGCCGGTGTCGGCGCATCGCTGCACCATCTGCTGTGCTTCTATCGGAATCGTCCGTTTGCG
CTGCTGGCGGCACACAAGAAGCGGATCGCCGACCGCCTGACCGACCTGCTGCTGCTGGCGGCGGCGGCAATGGCGTGGTC
GCAAGTGGGCAGCGGGACGTTCGCCGCACTTCGAGAGCATATTGCGATGTATGGCGCGTCGGCGCCCTTGCAGGCGAGTG
CGGTCTGCCTGGTACTGGCGATCGTCTTGCGCACGGCGCTCTTTCCCGTCCACGGGTGGTTGATTCAGGTCATGGAGGCG
CCGACGCCGGTCTCGGCACTGCTGCATGCCGGCGTCGTCAATCTTGCCGGCTACCTGCTGATGCGCTTCGCACCGCTGCT
TGCGGTCGCCCCTGCCGCGCGCTGGCTGCTGGTCGCATTCGGCCTCGGCACCGCGTTCCTGGCCGGGTTCGTCATGCTGA
CGCGCATCAGTATCAAAGTGCGTTTGGCGTGGTCCACCGTGGCACAGATGGGCTTCATGGTGCTCGAGTGCGGCCTCGGC
CTGTATCAGCTGGCCGCGCTGCACCTGATCGGCCACTCGCTCTACAAGGCCCACAGTTTTCTTGCCGCGTCGTCTGCGGT
GCAGGACACCCGGCTTCGGATCATGCGCGGTCGGAGCGACGCGGCGCCCCTCAGCCTGGTGCTTGCACCGGCCGGCAGCC
TCGCCGTCGTCGTGCTCGTGCAGGCGCTTCTGCCGTTCGGCAACTGGCCATGGTGGTGGAGCGGCATCCTTGCCCTGGCA
TGGGCGCCGCTACTCTGGATACCGCTGGCCCAAGGCAATGCGTTCGCCCGCGCGGGGACGCACCTATTCTTCGGTGCCGG
GCTGGTCGCGGTCTTCACGGCCGTCGCCACGCTCGCGCACAAACTTCCGCTCGGCGTCACGGATACCCCGTTCCGCATCG
CGGGCCCGGTCGCGCTAGCGGGCATCGCCCTGCTCTATCTCGGCCTGGCGACGCTGCAGCGCAGCCCGCAACGCTTTCCG
ACATGGTGGCGGTGGAGCTACGCGGGATTCTATGTCGACGAGTTCTACACGCGCTTTGCCCTGCGCGTCTGGCCGGCTCG
CTGGGCGCCGCGCGACGCCGCCCGAGGGCCGCGGCCGGCAGCGGGCGCGTCGCCCGTTCTCACCGAGCAATGA

Upstream 100 bases:

>100_bases
CATCGTTCTCTTTTATCAATGTATTCGTTCTATAAAATATATTTTACAGAATAATTGGCGTCGGACATCATCTATCGCAT
TGTTTTGCGAGAGGTTCGCC

Downstream 100 bases:

>100_bases
GCCGCTTATCCGGAGAATCACATGAGCATTGCAACACTCGAAAAAGGTGATGAGCGCCGCGCGGCCCGCTCGGCAGACGA
GGCCGCCCGCTCCGCGAACA

Product: NADH dehydrogenase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MIPTHLVLFLCALAPAALMSTAAGRLAWRPDDGRDVWKPFHRLAAVALLIVLIQPVVLATLHERAAAHEGGWTAWIEVTP
LAAWMAALVQLLGTVIGVFSGRYLQGEPGQPRYAAALAGVLASVHVLLLSGHWLLLIAAWAGVGASLHHLLCFYRNRPFA
LLAAHKKRIADRLTDLLLLAAAAMAWSQVGSGTFAALREHIAMYGASAPLQASAVCLVLAIVLRTALFPVHGWLIQVMEA
PTPVSALLHAGVVNLAGYLLMRFAPLLAVAPAARWLLVAFGLGTAFLAGFVMLTRISIKVRLAWSTVAQMGFMVLECGLG
LYQLAALHLIGHSLYKAHSFLAASSAVQDTRLRIMRGRSDAAPLSLVLAPAGSLAVVVLVQALLPFGNWPWWWSGILALA
WAPLLWIPLAQGNAFARAGTHLFFGAGLVAVFTAVATLAHKLPLGVTDTPFRIAGPVALAGIALLYLGLATLQRSPQRFP
TWWRWSYAGFYVDEFYTRFALRVWPARWAPRDAARGPRPAAGASPVLTEQ

Sequences:

>Translated_530_residues
MIPTHLVLFLCALAPAALMSTAAGRLAWRPDDGRDVWKPFHRLAAVALLIVLIQPVVLATLHERAAAHEGGWTAWIEVTP
LAAWMAALVQLLGTVIGVFSGRYLQGEPGQPRYAAALAGVLASVHVLLLSGHWLLLIAAWAGVGASLHHLLCFYRNRPFA
LLAAHKKRIADRLTDLLLLAAAAMAWSQVGSGTFAALREHIAMYGASAPLQASAVCLVLAIVLRTALFPVHGWLIQVMEA
PTPVSALLHAGVVNLAGYLLMRFAPLLAVAPAARWLLVAFGLGTAFLAGFVMLTRISIKVRLAWSTVAQMGFMVLECGLG
LYQLAALHLIGHSLYKAHSFLAASSAVQDTRLRIMRGRSDAAPLSLVLAPAGSLAVVVLVQALLPFGNWPWWWSGILALA
WAPLLWIPLAQGNAFARAGTHLFFGAGLVAVFTAVATLAHKLPLGVTDTPFRIAGPVALAGIALLYLGLATLQRSPQRFP
TWWRWSYAGFYVDEFYTRFALRVWPARWAPRDAARGPRPAAGASPVLTEQ
>Mature_530_residues
MIPTHLVLFLCALAPAALMSTAAGRLAWRPDDGRDVWKPFHRLAAVALLIVLIQPVVLATLHERAAAHEGGWTAWIEVTP
LAAWMAALVQLLGTVIGVFSGRYLQGEPGQPRYAAALAGVLASVHVLLLSGHWLLLIAAWAGVGASLHHLLCFYRNRPFA
LLAAHKKRIADRLTDLLLLAAAAMAWSQVGSGTFAALREHIAMYGASAPLQASAVCLVLAIVLRTALFPVHGWLIQVMEA
PTPVSALLHAGVVNLAGYLLMRFAPLLAVAPAARWLLVAFGLGTAFLAGFVMLTRISIKVRLAWSTVAQMGFMVLECGLG
LYQLAALHLIGHSLYKAHSFLAASSAVQDTRLRIMRGRSDAAPLSLVLAPAGSLAVVVLVQALLPFGNWPWWWSGILALA
WAPLLWIPLAQGNAFARAGTHLFFGAGLVAVFTAVATLAHKLPLGVTDTPFRIAGPVALAGIALLYLGLATLQRSPQRFP
TWWRWSYAGFYVDEFYTRFALRVWPARWAPRDAARGPRPAAGASPVLTEQ

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=286, Percent_Identity=30.4195804195804, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788614, Length=289, Percent_Identity=32.5259515570934, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788831, Length=173, Percent_Identity=35.2601156069364, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788829, Length=304, Percent_Identity=26.9736842105263, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 56955; Mature: 56955

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPTHLVLFLCALAPAALMSTAAGRLAWRPDDGRDVWKPFHRLAAVALLIVLIQPVVLAT
CCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHERAAAHEGGWTAWIEVTPLAAWMAALVQLLGTVIGVFSGRYLQGEPGQPRYAAALAGV
HHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LASVHVLLLSGHWLLLIAAWAGVGASLHHLLCFYRNRPFALLAAHKKRIADRLTDLLLLA
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AAAMAWSQVGSGTFAALREHIAMYGASAPLQASAVCLVLAIVLRTALFPVHGWLIQVMEA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTPVSALLHAGVVNLAGYLLMRFAPLLAVAPAARWLLVAFGLGTAFLAGFVMLTRISIKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
RLAWSTVAQMGFMVLECGLGLYQLAALHLIGHSLYKAHSFLAASSAVQDTRLRIMRGRSD
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPLSLVLAPAGSLAVVVLVQALLPFGNWPWWWSGILALAWAPLLWIPLAQGNAFARAGT
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHCCC
HLFFGAGLVAVFTAVATLAHKLPLGVTDTPFRIAGPVALAGIALLYLGLATLQRSPQRFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TWWRWSYAGFYVDEFYTRFALRVWPARWAPRDAARGPRPAAGASPVLTEQ
CCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIPTHLVLFLCALAPAALMSTAAGRLAWRPDDGRDVWKPFHRLAAVALLIVLIQPVVLAT
CCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHERAAAHEGGWTAWIEVTPLAAWMAALVQLLGTVIGVFSGRYLQGEPGQPRYAAALAGV
HHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
LASVHVLLLSGHWLLLIAAWAGVGASLHHLLCFYRNRPFALLAAHKKRIADRLTDLLLLA
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AAAMAWSQVGSGTFAALREHIAMYGASAPLQASAVCLVLAIVLRTALFPVHGWLIQVMEA
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTPVSALLHAGVVNLAGYLLMRFAPLLAVAPAARWLLVAFGLGTAFLAGFVMLTRISIKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
RLAWSTVAQMGFMVLECGLGLYQLAALHLIGHSLYKAHSFLAASSAVQDTRLRIMRGRSD
EEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPLSLVLAPAGSLAVVVLVQALLPFGNWPWWWSGILALAWAPLLWIPLAQGNAFARAGT
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHCCC
HLFFGAGLVAVFTAVATLAHKLPLGVTDTPFRIAGPVALAGIALLYLGLATLQRSPQRFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TWWRWSYAGFYVDEFYTRFALRVWPARWAPRDAARGPRPAAGASPVLTEQ
CCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]