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The map label for this gene is ilvX [H]

Identifier: 238023567

GI number: 238023567

Start: 70714

End: 72294

Strand: Direct

Name: ilvX [H]

Synonym: bglu_2g00690

Alternate gene names: 238023567

Gene position: 70714-72294 (Clockwise)

Preceding gene: 238023564

Following gene: 238023568

Centisome position: 2.5

GC content: 73.31

Gene sequence:

>1581_bases
ATGAACCTTCCACAGTTGCACCGCGATACCGAAGCAAGCAGCGGGGCGAGCGTCGTGCTCACCACGGCGCGAGACGCCGG
CGTCGGCGTCTGTTTCGCCAATCCCGGCACCACCGAGATGCCGTTCGTCGGTGCGCTCGACACCGTGGCGGGCGTGCGCC
CGATCCTCGGCCTGTTCGAGGGCGTGTGCACGGGAGCCGCGGACGGCTACGGCCGCATGGCCGGCAAGCCCGCGATGACG
CTGCTGCACCTCGGCCCCGGCCACGCCAACGGCATTGCCAATCTGCACAATGCGCGCCGCGCCCGCACGCCGATCCTGAA
CATCGTCGGCGATCACACGCGCGCGCATCTGAAGTACGACGCGCCGCTGACGTCCGACATCGAATCGCTCGCGCGCCCGG
TGTCGGTGTGGTACCGCAGCGTGGCGCGCGACACCGATCTCGCGACCGATACGGCCGACGCGATTGCCGCTGCGATGGGC
GCGCAGCGCGGCGTCGCGACGCTGGTCCTGCCCGTCGATCTCCAGTCCGCGCGGATACGCGGCGGGGCGTCGCCCGCGGT
CGTGCGCCCGGCTGCGGCCTCGTTCGACCCGGAGCGCGTCGAGCGGGTGGCCGATCTGCTGCTCGGCGGCGATCGCGCCG
TCCTGCTGCTGGGCCAGCGCGCCTTGTCGGCGCGCGGGCAGCGCGCGGCCGCCCGCATCGCGGCGGCGACCGGTGCGGCC
TGCTTCAGCGAGACGTTTCCGGCGCGGGCCGAGCGCGGCGGCGGCCTGCCCGACATCGACCGCCTGCCTTATTTCCCCGA
GCCGGCGCTGGCGGCGCTGGCGGGCCGGCGCGTGGTGCTGGCCGGCGCGCTGGCGCCCGTCACTTACTTCGGCTACGACG
GGATTCCCGGCGAGCTCGCGCGGCCGGACGATATCGTCATGCTGGCGGCCCCGGGCGACGCGGCGGACGACGCGCTGGAG
GCGCTGGCCGAGCGGCTCGGCGCGGCAGCGAATGACCAGGGCGTGTGCGTCGAGCGCTGGCAGGTGGCGGGCGGGCCGCT
CACGCCGCAGGCCGTCGGCTGCGTGCTCGCGAATGCGCTGCCCGATAACGCGATCGTCTCGATCGAGGGCGGCACCTGCG
GCTACCCGTTCGTGACCGCGTCGGCGCGCGCGCGTCGCCATACCATTCTCACGAATACGGGCGGCGCGATCGGCCAGGGC
CTGCCGGTGGCGCTCGGCGCGGCCGTCGCATGCCCGGACCGCCGCGTCTTTGCGCTGCAGTCCGACGGCAGTGCGCAGTA
CACGATCCAGTCGCTGTGGACCATGGCGCGCGAGCGCTTGCCGATCGTGATGCTGATCGCCTCGAACCGTCGCTACGGCA
TCCTGCAGACCGAGCTCGCGCGCAGCGGCCTGCCGGCCGACACGCCGCACGCGAGCCGCCTGACGCAACTGGACGATCCG
CCGATCGACTGGCTCGCGCTGTCGCGCGGCTATGGCGTGCCGGCCCGACGCGCGAGCGACACGCAGGCGCTGGTGCGAGC
GCTCGACGACGCGCTCGCTCATGCCGACGGGCCGACACTCATCGAAATGCAGTTGTCCTGA

Upstream 100 bases:

>100_bases
AAACGTGGATCGAAACAAGTGGCCAAGACGACGTGCGTAGTTTGACAAATCCCGTATTTCGGGATTAAATGAAAAACAGT
CAAACTCGCCGAGCCGTCCC

Downstream 100 bases:

>100_bases
TCGCGCCGCGCGATCGTTTCCTCAATCCCGACTCTCACCTCTCATCGACATGGATCTGCAACTGCACGGAAAAGCCGCGT
TCATCACCGGCGGCAGCATG

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNLPQLHRDTEASSGASVVLTTARDAGVGVCFANPGTTEMPFVGALDTVAGVRPILGLFEGVCTGAADGYGRMAGKPAMT
LLHLGPGHANGIANLHNARRARTPILNIVGDHTRAHLKYDAPLTSDIESLARPVSVWYRSVARDTDLATDTADAIAAAMG
AQRGVATLVLPVDLQSARIRGGASPAVVRPAAASFDPERVERVADLLLGGDRAVLLLGQRALSARGQRAAARIAAATGAA
CFSETFPARAERGGGLPDIDRLPYFPEPALAALAGRRVVLAGALAPVTYFGYDGIPGELARPDDIVMLAAPGDAADDALE
ALAERLGAAANDQGVCVERWQVAGGPLTPQAVGCVLANALPDNAIVSIEGGTCGYPFVTASARARRHTILTNTGGAIGQG
LPVALGAAVACPDRRVFALQSDGSAQYTIQSLWTMARERLPIVMLIASNRRYGILQTELARSGLPADTPHASRLTQLDDP
PIDWLALSRGYGVPARRASDTQALVRALDDALAHADGPTLIEMQLS

Sequences:

>Translated_526_residues
MNLPQLHRDTEASSGASVVLTTARDAGVGVCFANPGTTEMPFVGALDTVAGVRPILGLFEGVCTGAADGYGRMAGKPAMT
LLHLGPGHANGIANLHNARRARTPILNIVGDHTRAHLKYDAPLTSDIESLARPVSVWYRSVARDTDLATDTADAIAAAMG
AQRGVATLVLPVDLQSARIRGGASPAVVRPAAASFDPERVERVADLLLGGDRAVLLLGQRALSARGQRAAARIAAATGAA
CFSETFPARAERGGGLPDIDRLPYFPEPALAALAGRRVVLAGALAPVTYFGYDGIPGELARPDDIVMLAAPGDAADDALE
ALAERLGAAANDQGVCVERWQVAGGPLTPQAVGCVLANALPDNAIVSIEGGTCGYPFVTASARARRHTILTNTGGAIGQG
LPVALGAAVACPDRRVFALQSDGSAQYTIQSLWTMARERLPIVMLIASNRRYGILQTELARSGLPADTPHASRLTQLDDP
PIDWLALSRGYGVPARRASDTQALVRALDDALAHADGPTLIEMQLS
>Mature_526_residues
MNLPQLHRDTEASSGASVVLTTARDAGVGVCFANPGTTEMPFVGALDTVAGVRPILGLFEGVCTGAADGYGRMAGKPAMT
LLHLGPGHANGIANLHNARRARTPILNIVGDHTRAHLKYDAPLTSDIESLARPVSVWYRSVARDTDLATDTADAIAAAMG
AQRGVATLVLPVDLQSARIRGGASPAVVRPAAASFDPERVERVADLLLGGDRAVLLLGQRALSARGQRAAARIAAATGAA
CFSETFPARAERGGGLPDIDRLPYFPEPALAALAGRRVVLAGALAPVTYFGYDGIPGELARPDDIVMLAAPGDAADDALE
ALAERLGAAANDQGVCVERWQVAGGPLTPQAVGCVLANALPDNAIVSIEGGTCGYPFVTASARARRHTILTNTGGAIGQG
LPVALGAAVACPDRRVFALQSDGSAQYTIQSLWTMARERLPIVMLIASNRRYGILQTELARSGLPADTPHASRLTQLDDP
PIDWLALSRGYGVPARRASDTQALVRALDDALAHADGPTLIEMQLS

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=23.8779174147217, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 54595; Mature: 54595

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPQLHRDTEASSGASVVLTTARDAGVGVCFANPGTTEMPFVGALDTVAGVRPILGLFE
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
GVCTGAADGYGRMAGKPAMTLLHLGPGHANGIANLHNARRARTPILNIVGDHTRAHLKYD
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEC
APLTSDIESLARPVSVWYRSVARDTDLATDTADAIAAAMGAQRGVATLVLPVDLQSARIR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHCC
GGASPAVVRPAAASFDPERVERVADLLLGGDRAVLLLGQRALSARGQRAAARIAAATGAA
CCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHH
CFSETFPARAERGGGLPDIDRLPYFPEPALAALAGRRVVLAGALAPVTYFGYDGIPGELA
HHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEHHHHHHEECCCCCCCCCC
RPDDIVMLAAPGDAADDALEALAERLGAAANDQGVCVERWQVAGGPLTPQAVGCVLANAL
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
PDNAIVSIEGGTCGYPFVTASARARRHTILTNTGGAIGQGLPVALGAAVACPDRRVFALQ
CCCCEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCHHHHHEEECCCCEEEEEE
SDGSAQYTIQSLWTMARERLPIVMLIASNRRYGILQTELARSGLPADTPHASRLTQLDDP
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHCCCCCCCCCHHHHCCCCCC
PIDWLALSRGYGVPARRASDTQALVRALDDALAHADGPTLIEMQLS
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MNLPQLHRDTEASSGASVVLTTARDAGVGVCFANPGTTEMPFVGALDTVAGVRPILGLFE
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
GVCTGAADGYGRMAGKPAMTLLHLGPGHANGIANLHNARRARTPILNIVGDHTRAHLKYD
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEEC
APLTSDIESLARPVSVWYRSVARDTDLATDTADAIAAAMGAQRGVATLVLPVDLQSARIR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHCC
GGASPAVVRPAAASFDPERVERVADLLLGGDRAVLLLGQRALSARGQRAAARIAAATGAA
CCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHH
CFSETFPARAERGGGLPDIDRLPYFPEPALAALAGRRVVLAGALAPVTYFGYDGIPGELA
HHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEEHHHHHHEECCCCCCCCCC
RPDDIVMLAAPGDAADDALEALAERLGAAANDQGVCVERWQVAGGPLTPQAVGCVLANAL
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
PDNAIVSIEGGTCGYPFVTASARARRHTILTNTGGAIGQGLPVALGAAVACPDRRVFALQ
CCCCEEEECCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCHHHHHEEECCCCEEEEEE
SDGSAQYTIQSLWTMARERLPIVMLIASNRRYGILQTELARSGLPADTPHASRLTQLDDP
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHCCCCCCCCCHHHHCCCCCC
PIDWLALSRGYGVPARRASDTQALVRALDDALAHADGPTLIEMQLS
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]