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The map label for this gene is dmlA [H]

Identifier: 238023515

GI number: 238023515

Start: 14926

End: 16011

Strand: Reverse

Name: dmlA [H]

Synonym: bglu_2g00150

Alternate gene names: 238023515

Gene position: 16011-14926 (Counterclockwise)

Preceding gene: 238023517

Following gene: 238023512

Centisome position: 0.57

GC content: 67.68

Gene sequence:

>1086_bases
ATGAGCGAGAAGATCTACAGGATTGCGGTGATTCCCGGCGACGGCATCGGCCGCGAGGTGATGCCGGAAGGCATGCGGGT
GCTCGATGCGGTCACGCGGCGCTTCGGCATCCGCTTCGAGGCGACGCCGATCGACTGGGCCAGCTGCGATTACTACGCGC
AGCACGGCCAGATGATGCCGGAGGATTGGAAAACGCAGCTTTCGGGAATGGACGCATTGCTGTTCGGGGCGGTCGGCTGG
CCCGACACGGTGCCCGATCACATCTCGCTGTGGGGCTCGCTGCTGAAGTTCCGCCGCGAGTTCGACCAGTATGTGAACCT
GCGGCCGGCGCGGCTGTTCGAGGGCGTGCCGAGCCCGCTCGCGAACCGCAAGGCCGGCGACATCGATTTCATGATCGTGC
GCGAGAACACCGAGGGCGAATATTCATCGGTGGGCGGCGCAATGTTCGAGGGCACCGAGCGCGAGTTCGTGGTGCAGCAG
GCGGTGTTCACGCGCACCGGCACCGAGCGCGTGATGAAGTTCGCGTTCGAGCTCGCGCAGCGCCGCGCGAAGCAGCTGAC
CGTCGCGACCAAGAGCAACGGCATCGCGATCAGCATGCCGTGGTGGGACGCGCGCGCGGCGGAAATGGCGGCCCGCTATC
CCGAGGTGAAGTGGGACAAGCAGCACATCGACATCCTGTGCGCGCGCTTCGTGCTGAACCCGGACCGCTTCGACGTGGTG
GTGGCCTCGAACCTGTTCGGCGACATCCTCTCCGATCTCGGGCCGGCCTGCACCGGCACCATCGGCATCGCGCCGTCGGC
GAACCTGAACCCGGACCGGCGGTTCCCGTCGCTGTTCGAGCCCGTGCATGGCTCGGCGCCCGACATCGCGGGGCGCCACA
TCGCGAACCCGATCGCGATGATCTGGTCGGCCGCGATGATGGTCGACTTCCTCGGCCACGGCAGCGGCGTCGAACGCGAG
GCGCACGACGCGATCCTCGCCGCGATCGAACATGTGTTGAAGCACGGCCCGCGCACGGGCGACCTGGGCGGCCAGGCCGG
CACGCAGGAAGTGGGCGAGGCAGTGGTCGCGCAGCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
AATTCTAATCCTGTACGGAATCAATACACTAGGCGAATGCGGTGCGTGCCGGGGTCTGTCGGCGCGCACCTTGGATCACG
ACGACGAGGAGGCTCGGGCG

Downstream 100 bases:

>100_bases
GCGGACGCGTAGGGCGCGCTGCCGAAACACGAACGGTGCGGCCGCGGCGGGCGCCGGCCGCACCGTTTGCCGGCTCCCGA
AAAGACTCACCTCAGCGCGC

Product: Tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSEKIYRIAVIPGDGIGREVMPEGMRVLDAVTRRFGIRFEATPIDWASCDYYAQHGQMMPEDWKTQLSGMDALLFGAVGW
PDTVPDHISLWGSLLKFRREFDQYVNLRPARLFEGVPSPLANRKAGDIDFMIVRENTEGEYSSVGGAMFEGTEREFVVQQ
AVFTRTGTERVMKFAFELAQRRAKQLTVATKSNGIAISMPWWDARAAEMAARYPEVKWDKQHIDILCARFVLNPDRFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPSLFEPVHGSAPDIAGRHIANPIAMIWSAAMMVDFLGHGSGVERE
AHDAILAAIEHVLKHGPRTGDLGGQAGTQEVGEAVVAQLLG

Sequences:

>Translated_361_residues
MSEKIYRIAVIPGDGIGREVMPEGMRVLDAVTRRFGIRFEATPIDWASCDYYAQHGQMMPEDWKTQLSGMDALLFGAVGW
PDTVPDHISLWGSLLKFRREFDQYVNLRPARLFEGVPSPLANRKAGDIDFMIVRENTEGEYSSVGGAMFEGTEREFVVQQ
AVFTRTGTERVMKFAFELAQRRAKQLTVATKSNGIAISMPWWDARAAEMAARYPEVKWDKQHIDILCARFVLNPDRFDVV
VASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPSLFEPVHGSAPDIAGRHIANPIAMIWSAAMMVDFLGHGSGVERE
AHDAILAAIEHVLKHGPRTGDLGGQAGTQEVGEAVVAQLLG
>Mature_360_residues
SEKIYRIAVIPGDGIGREVMPEGMRVLDAVTRRFGIRFEATPIDWASCDYYAQHGQMMPEDWKTQLSGMDALLFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPARLFEGVPSPLANRKAGDIDFMIVRENTEGEYSSVGGAMFEGTEREFVVQQA
VFTRTGTERVMKFAFELAQRRAKQLTVATKSNGIAISMPWWDARAAEMAARYPEVKWDKQHIDILCARFVLNPDRFDVVV
ASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPSLFEPVHGSAPDIAGRHIANPIAMIWSAAMMVDFLGHGSGVEREA
HDAILAAIEHVLKHGPRTGDLGGQAGTQEVGEAVVAQLLG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=365, Percent_Identity=32.3287671232877, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI28178816, Length=365, Percent_Identity=27.9452054794521, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=27.8551532033426, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=27.1232876712329, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178838, Length=311, Percent_Identity=27.9742765273312, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI28178819, Length=203, Percent_Identity=30.0492610837438, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=69.2737430167598, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=39.6694214876033, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1787381, Length=380, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=367, Percent_Identity=32.6975476839237, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=29.3296089385475, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.683615819209, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=359, Percent_Identity=24.7910863509749, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322097, Length=381, Percent_Identity=35.4330708661417, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=33.5164835164835, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=33.7837837837838, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=367, Percent_Identity=28.3378746594005, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643268, Length=366, Percent_Identity=33.0601092896175, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24643270, Length=366, Percent_Identity=33.0601092896175, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=31.8681318681319, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI161078637, Length=359, Percent_Identity=28.133704735376, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078635, Length=359, Percent_Identity=28.133704735376, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=359, Percent_Identity=28.133704735376, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650122, Length=359, Percent_Identity=28.133704735376, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078639, Length=356, Percent_Identity=29.2134831460674, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281362242, Length=359, Percent_Identity=27.2980501392758, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648872, Length=359, Percent_Identity=27.2980501392758, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI20130355, Length=363, Percent_Identity=26.7217630853994, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39758; Mature: 39627

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKIYRIAVIPGDGIGREVMPEGMRVLDAVTRRFGIRFEATPIDWASCDYYAQHGQMMP
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHCCCCCC
EDWKTQLSGMDALLFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPARLFEGVPSPL
HHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
ANRKAGDIDFMIVRENTEGEYSSVGGAMFEGTEREFVVQQAVFTRTGTERVMKFAFELAQ
CCCCCCCEEEEEEEECCCCCHHHCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRAKQLTVATKSNGIAISMPWWDARAAEMAARYPEVKWDKQHIDILCARFVLNPDRFDVV
HHHHHEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPSLFEPVHGSAPDIAGRHIANPIAM
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
IWSAAMMVDFLGHGSGVEREAHDAILAAIEHVLKHGPRTGDLGGQAGTQEVGEAVVAQLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
G
C
>Mature Secondary Structure 
SEKIYRIAVIPGDGIGREVMPEGMRVLDAVTRRFGIRFEATPIDWASCDYYAQHGQMMP
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHCCCCCC
EDWKTQLSGMDALLFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPARLFEGVPSPL
HHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
ANRKAGDIDFMIVRENTEGEYSSVGGAMFEGTEREFVVQQAVFTRTGTERVMKFAFELAQ
CCCCCCCEEEEEEEECCCCCHHHCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRAKQLTVATKSNGIAISMPWWDARAAEMAARYPEVKWDKQHIDILCARFVLNPDRFDVV
HHHHHEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPSLFEPVHGSAPDIAGRHIANPIAM
EHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHH
IWSAAMMVDFLGHGSGVEREAHDAILAAIEHVLKHGPRTGDLGGQAGTQEVGEAVVAQLL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]