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The map label for this gene is alkJ [H]

Identifier: 237814374

GI number: 237814374

Start: 3997245

End: 3998930

Strand: Reverse

Name: alkJ [H]

Synonym: GBP346_A4172

Alternate gene names: 237814374

Gene position: 3998930-3997245 (Counterclockwise)

Preceding gene: 237814376

Following gene: 237814372

Centisome position: 97.57

GC content: 69.4

Gene sequence:

>1686_bases
GTGACTACCGAACGTACGCTCGAAGGCGAATTCGATTATGTGATCGTCGGCGCCGGCACGGCGGGCTGCGTGCTCGCGAA
CCGGCTCACCGAGGATCCGGAGGTGACCGTGCTGCTGCTCGAAGCCGGCGGCAGGGACGACTATCACTGGATCCACATCC
CGGTCGGCTATCTGTATTGCATCGGCAATCCGCGCACCGACTGGCTCTACAAGACCGAGCCCGAAGCGGGCCTGAACGGC
CGCGCGCTGTCGTATCCGCGCGGGCGCGTGCTGGGCGGCTCGTCGTCGATCAACGGAATGATCTACATGCGCGGCCAGCG
CGGCGATTACGACGACTGGGCGCGCGCCACGGGCGACGCGGGCTGGTCGTGGGACAGTGTGCTGCCCGTCTTCAGGCGCA
GCGAGGATCATCATGCGGGCGCGACCGACATGCACGGCGCGGGCGGCATGTGGCGCGTCGAGAAGCAGCGGCTGCGCTGG
GAGATTCTCGAGGCATTCTCGCAGGCCGCGCAGCAGACGGGCATTCCGGCCACCGACGATTTCAACCGCGGCGACAACAC
GGGCGTCGGCTATTTCGAAGTCAATCAGAAGCGCGGCATTCGCTGGAACGCGTCGAAGGCGTTCTTGCGTCCCGCGCTCG
CGCGGCCGAACCTCACGGTGATCACCGGCGCGCAGGCCGAGCGGCTCGTGTTCGACGGCAAGCGCTGCGCGGGGGTCGAA
TATCGCGGCGGCGGCGCGCCGTTCGTCGCGCGCGCGCGCGTCGAGGTGCTCGTCGCGTCGGGCGCGGTGAATTCGCCGCA
GTTGCTCGAATTGTCGGGCATCGGCGACGGCAGCCGGCTGCAGGCGCTCGGCATCGGCGTCATCGCGGATCTGCGCGGCG
TCGGCGAAAATCTTCAGGATCACTTGCAGTTGCGCATGGCGTTTCGCGTGCGCGGCGTGCGCACGCTGAACACGCTGTCC
GCGCACTGGTGGGGCAAGCTGTGGATCGGCGCGCAATACGCGCTGATGCAGCGCGGGCCGATGTCGATGGCGCCGTCGCA
ATTGGGCGCGTTCGCGAAATCGGACCCGAACGATCCGGCACTCGCGCAGCCCGATCTCGAATATCACGTGCAGCCGCTGT
CGCTCGAGCGCTTCGGCGAGCCGCTGCATCGCTTCAACGCGTTCACCGCGTCGGTCTGCCATCTGCGGCCGACGTCGCGC
GGCAGCGTCCATGCGGCGTCGCCGGATCCGGCGCGCGCGCCGTCGATTGCGCCGAACTATCTGTCGACCGATTACGATCG
CCATGTCGCGGCGAACGCGCTGCGCCTGACGCGCCGGATCGCCTCGGCGCCCGCGCTCGCGCGCTATGCGCCCGAAGAAA
TCCTGCCGGGCGCCCGGTATGTGAGCGAAGCGGAGCTGATCGCCGCGGCGGGCGCCGTGGGCACGACGATTTTCCATCCG
GTCGGCACGTGCCGGATGGGGCGCGCCGACGATCCGGACGCCGTCGTCGACAGCCGCCTGCGCGTGCGCGGCGTGACGGG
GCTGCGGATCGTCGACGCGTCGGTGATGCCGACGATCACGTCGGGCAACACGAACTCCCCGACGCTGATGATCGCCGAGC
GCGCGAGCGACATGATTCGTGCGGATCGCCGCGGCGCATCGGAGCGCGGCGCGAGCGCGCGCGCCGAAGCCGTGCTGCCG
GCGTAA

Upstream 100 bases:

>100_bases
GCGCGCCGGCGATCTGCGATCGGCGCGCGGCTCGGCGCGAAGGCGCGCGACGGCGGTCCGCCAAGAAACCGCCGCATCGG
AATCGAGGAGACGGCAGATC

Downstream 100 bases:

>100_bases
AAGTGACACGATCCATTGAAAGAATCGGCCACGAATCGAAATAAATAACCGGCTGAGGGTTAACCCAAAAGCAAAGCGGC
ACTCTCGCGCGCACTCGATC

Product: choline dehydrogenase, (CHD)(CDH)

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPEVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YRGGGAPFVARARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALAQPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLP
A

Sequences:

>Translated_561_residues
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPEVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YRGGGAPFVARARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALAQPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLP
A
>Mature_560_residues
TTERTLEGEFDYVIVGAGTAGCVLANRLTEDPEVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNGR
ALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWE
ILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVEY
RGGGAPFVARARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLSA
HWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALAQPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRG
SVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHPV
GTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLPA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=41.82156133829, Blast_Score=397, Evalue=1e-110,
Organism=Escherichia coli, GI1786503, Length=538, Percent_Identity=39.4052044609665, Blast_Score=364, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17532301, Length=558, Percent_Identity=33.8709677419355, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI45549471, Length=571, Percent_Identity=37.3029772329247, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI45551458, Length=571, Percent_Identity=37.3029772329247, Blast_Score=334, Evalue=8e-92,
Organism=Drosophila melanogaster, GI24642055, Length=582, Percent_Identity=37.1134020618557, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=35.6521739130435, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=36.0281195079086, Blast_Score=312, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24642048, Length=577, Percent_Identity=34.315424610052, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI18859995, Length=578, Percent_Identity=35.121107266436, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24650267, Length=568, Percent_Identity=34.1549295774648, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=34.5008756567426, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI17137792, Length=556, Percent_Identity=33.6330935251799, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=32.8097731239092, Blast_Score=267, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=32.1116928446771, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI18859993, Length=574, Percent_Identity=30.4878048780488, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24642035, Length=303, Percent_Identity=40.2640264026403, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24642057, Length=602, Percent_Identity=28.9036544850498, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=28.8659793814433, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60789; Mature: 60658

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPEVTVLLLEAGGRDDYHWIHIPVGYLYC
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHCCCC
GWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEE
YRGGGAPFVARARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQD
ECCCCCCEEEEEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LAQPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSVHAASPDPARAPSIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCCCCCCC
LSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGASERGASARAEAVLPA
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTERTLEGEFDYVIVGAGTAGCVLANRLTEDPEVTVLLLEAGGRDDYHWIHIPVGYLYC
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHCCCC
GWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEE
YRGGGAPFVARARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQD
ECCCCCCEEEEEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LAQPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSVHAASPDPARAPSIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCCCCCCC
LSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRIVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGASERGASARAEAVLPA
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]