BacMap

Definition	Burkholderia pseudomallei MSHR346 chromosome I, complete genome.
Accession	NC_012695
Length	4,098,576

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The map label for this gene is braG [H]

Identifier: 237814371

GI number: 237814371

Start: 3995214

End: 3995969

Strand: Reverse

Name: braG [H]

Synonym: GBP346_A4169

Alternate gene names: 237814371

Gene position: 3995969-3995214 (Counterclockwise)

Preceding gene: 237814372

Following gene: 237814370

Centisome position: 97.5

GC content: 68.25

Gene sequence:

>756_bases
ATGAACCACAGCGAACGGGAAGAGCGCGAGTTGAACAGCGTCGAAAGCGGCACGCCCGCGCTCGCGCTCGCGGGGCTCGA
GGCGTGGTACGGCGAATCGCACATCCTGCACGGCGTCGATCTGACCGTGCACCGCGGCGAGGTCGTCACGCTGCTCGGCC
GCAACGGCGCGGGCCGCACGACGACGCTGCGCGCGATCATGGGGCTCACGGGCCGCCGCGAAGGCTCGATCAGGATCGCG
GGCCACGAGACGATCGGCCTGCCGACGCACAAGATCGCGCATTACGGCGTCGGCTACTGCCCGGAGGAGCGCGGGATCTT
CTCGAGCCTGTCGTGCGAGGAGAACCTGCTGCTGCCGCCCGTGCTCGGCGATCGCAAGCGCGCGATGTCGCTCGAGGACA
TCTACGAGATGTTTCCGAACCTCGCGTCGCGGCGCGCCAGCCAGGGCACGCGGCTGTCGGGCGGCGAGCAGCAGATGCTC
GCGGTCGCGCGCATCCTGCGCACCGGCGCGAACCTGCTGTTGCTCGACGAGATCTCCGAGGGCCTCGCGCCCGTGATCGT
GCAGGCGCTCGCGAAGATGATCGTCACGCTCAAGGCGCGCGGCTACACGATCGTGATGGTCGAGCAGAATTTCCGTTTCG
CGGCGCCGCTCGCCGACCGTTTCTATGTGATGGAGCACGGCCGCATCGTCGAGCATTTCGGCGCGGCCGAGCTCGAGGGG
AAGATGCCCGTGCTGCATGATCTGCTCGGCGTATAG

Upstream 100 bases:

>100_bases
AGGTGCTCGCGCAAGGCACGTACGCGGAAGTGTCGAAGAACCCGCTCGTCGTCGAGGCGTACATGGGCAGCGCGGACGCG
GCGCTCGCGGGGGCGCACGC

Downstream 100 bases:

>100_bases
CGCCGCTTTGCCGTTCCCCCGATGTACATAACCTGACAACCACAACGCACTACACCAGGAGACGCCAATGAAATTGAAGA
CCCTTGCGCACGCCTGTCTT

Product: branched-chain amino acid ABC transporter, ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNHSEREERELNSVESGTPALALAGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRREGSIRIA
GHETIGLPTHKIAHYGVGYCPEERGIFSSLSCEENLLLPPVLGDRKRAMSLEDIYEMFPNLASRRASQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALAKMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAAELEG
KMPVLHDLLGV

Sequences:

>Translated_251_residues
MNHSEREERELNSVESGTPALALAGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRREGSIRIA
GHETIGLPTHKIAHYGVGYCPEERGIFSSLSCEENLLLPPVLGDRKRAMSLEDIYEMFPNLASRRASQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALAKMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAAELEG
KMPVLHDLLGV
>Mature_251_residues
MNHSEREERELNSVESGTPALALAGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRREGSIRIA
GHETIGLPTHKIAHYGVGYCPEERGIFSSLSCEENLLLPPVLGDRKRAMSLEDIYEMFPNLASRRASQGTRLSGGEQQML
AVARILRTGANLLLLDEISEGLAPVIVQALAKMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAAELEG
KMPVLHDLLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=200, Percent_Identity=31.5, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI47078218, Length=200, Percent_Identity=31.5, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI27881501, Length=217, Percent_Identity=29.4930875576037, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI30795238, Length=217, Percent_Identity=29.4930875576037, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI21536376, Length=195, Percent_Identity=26.1538461538462, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI105990541, Length=193, Percent_Identity=25.9067357512953, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI31657092, Length=236, Percent_Identity=25.4237288135593, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI150417984, Length=223, Percent_Identity=28.6995515695067, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=29.5336787564767, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI9961250, Length=225, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4505771, Length=225, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI9961252, Length=225, Percent_Identity=28.8888888888889, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=198, Percent_Identity=26.7676767676768, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI42741659, Length=216, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI27262624, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27262626, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI190343023, Length=220, Percent_Identity=29.0909090909091, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI9955963, Length=205, Percent_Identity=27.8048780487805, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=211, Percent_Identity=41.7061611374408, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1789593, Length=222, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789672, Length=227, Percent_Identity=30.3964757709251, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787792, Length=224, Percent_Identity=31.6964285714286, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788225, Length=225, Percent_Identity=34.2222222222222, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788450, Length=210, Percent_Identity=31.9047619047619, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787029, Length=219, Percent_Identity=27.8538812785388, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788897, Length=217, Percent_Identity=31.3364055299539, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787089, Length=226, Percent_Identity=30.5309734513274, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790525, Length=223, Percent_Identity=28.2511210762332, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=25.6, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=28.6919831223629, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790190, Length=224, Percent_Identity=25.4464285714286, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI48995001, Length=215, Percent_Identity=26.9767441860465, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787105, Length=248, Percent_Identity=27.0161290322581, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788761, Length=220, Percent_Identity=29.5454545454545, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081791, Length=220, Percent_Identity=28.1818181818182, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786872, Length=216, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786253, Length=224, Percent_Identity=30.3571428571429, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786398, Length=208, Percent_Identity=25.4807692307692, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789991, Length=231, Percent_Identity=28.1385281385281, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786319, Length=229, Percent_Identity=27.9475982532751, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790162, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787712, Length=220, Percent_Identity=28.1818181818182, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786703, Length=211, Percent_Identity=28.9099526066351, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=170, Percent_Identity=31.7647058823529, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790467, Length=209, Percent_Identity=29.1866028708134, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788644, Length=238, Percent_Identity=28.9915966386555, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787112, Length=213, Percent_Identity=31.4553990610329, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994943, Length=231, Percent_Identity=26.4069264069264, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788165, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786698, Length=170, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081709, Length=213, Percent_Identity=28.169014084507, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788472, Length=228, Percent_Identity=25.4385964912281, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=25.2293577981651, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788210, Length=231, Percent_Identity=23.8095238095238, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789751, Length=231, Percent_Identity=27.7056277056277, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790544, Length=225, Percent_Identity=25.3333333333333, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789873, Length=212, Percent_Identity=22.6415094339623, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87081834, Length=205, Percent_Identity=24.8780487804878, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=226, Percent_Identity=30.9734513274336, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=196, Percent_Identity=29.5918367346939, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=26.2222222222222, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=26.2222222222222, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=26.2222222222222, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=204, Percent_Identity=23.0392156862745, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984940, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71992285, Length=210, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17565938, Length=213, Percent_Identity=24.4131455399061, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648314, Length=207, Percent_Identity=28.5024154589372, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221512771, Length=214, Percent_Identity=26.6355140186916, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643674, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136662, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574150, Length=245, Percent_Identity=24.4897959183673, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007328, Length=245, Percent_Identity=24.4897959183673, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573573, Length=216, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573571, Length=216, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI85725262, Length=192, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585091, Length=220, Percent_Identity=24.5454545454545, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24650853, Length=185, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24650855, Length=185, Percent_Identity=27.027027027027, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27474; Mature: 27474

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSEREERELNSVESGTPALALAGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT
CCCCHHHHHHHHHHCCCCCCEEEHHHHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH
TTLRAIMGLTGRREGSIRIAGHETIGLPTHKIAHYGVGYCPEERGIFSSLSCEENLLLPP
HHHHHHHHCCCCCCCCEEEECCCEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VLGDRKRAMSLEDIYEMFPNLASRRASQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALAKMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAAELEG
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHEEEHHCCCHHHHCCCCCCCC
KMPVLHDLLGV
CCHHHHHHHCC
>Mature Secondary Structure
MNHSEREERELNSVESGTPALALAGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT
CCCCHHHHHHHHHHCCCCCCEEEHHHHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH
TTLRAIMGLTGRREGSIRIAGHETIGLPTHKIAHYGVGYCPEERGIFSSLSCEENLLLPP
HHHHHHHHCCCCCCCCEEEECCCEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VLGDRKRAMSLEDIYEMFPNLASRRASQGTRLSGGEQQMLAVARILRTGANLLLLDEISE
CCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC
GLAPVIVQALAKMIVTLKARGYTIVMVEQNFRFAAPLADRFYVMEHGRIVEHFGAAELEG
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHEEEHHCCCHHHHCCCCCCCC
KMPVLHDLLGV
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]