Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 237814227

GI number: 237814227

Start: 3853648

End: 3854478

Strand: Reverse

Name: metF [H]

Synonym: GBP346_A4022

Alternate gene names: 237814227

Gene position: 3854478-3853648 (Counterclockwise)

Preceding gene: 237814228

Following gene: 237814224

Centisome position: 94.04

GC content: 67.15

Gene sequence:

>831_bases
ATGAACCCGATCGAACTTTCCTTTGAATTCTTTCCGCCGAAGACGGCGGAAGGCGTCGACAAGCTGCGCGCGACGCGCGC
GCAGCTCGCGCCGCTCAAGCCGAAATTCGTGTCCGTCACGTTCGGCGCGGGCGGCTCGACGCAGCAAGGCACGCTCGACA
CCGTGCTCGACATGCAGAAGGCGGGGCTCGAAGCCGCGCCTCACCTGTCGTGCATCGGCTCGTCGAAGGACAGCCTGCGC
GCGATTCTCGACCAGTATCGCTCGCACGGCATCCGCCACATCGTCGCGTTGCGCGGCGATCTGCCTTCCGGCATGGGCGA
GGTGGGCGAGCTGCGCTACGCGTCGGAGCTCGTCAGCTTCATCCGCGCCGAGCATGGCGACTGGTTCCGGATCGAAGTGG
CCGCGTATCCCGAGTACCACCCGCAGGCGCGCTCGCCGAAGGCCGATCTCGAGAACTTCGCGCGCAAGGTGAAGGCGGGC
GCGAACGCCGCGATCACGCAGTATTTCTACAACGCGGACGCGTATTTCCGCTTCGTCGACGATGCGGCGAAGCTCGGCGT
CGACGTGCCGGTCGTGCCGGGCATCATGCCGATCACGAACTTCTCGCAACTGATGCGCTTCTCGGAGATGTGCGGCGCGG
AAGTGCCGCGCTGGGTCGCGCGCCGGCTCGAGAGCTTCGGCGACGACAAGGATTCGATCCGCGCGTTCGGCGCGGACGTC
GTCACGGGCCTTTGCCGGCGGCTCATCGACGCGGGCGTGCCCGGTCTGCACTTCTACACGCTCAACGGCGCGGCGGCGAC
GAAGACGGTCTGCGAGCGGCTGGGGCTCTGA

Upstream 100 bases:

>100_bases
CCGCATTCTTCGGTTCGATCCTGTACAGCATCGTGTCGTGGCTGCTGTCCGCCCTGATCTTCGGTCAGCGCAACATCGGC
TGACAGGTGATATCTGAATC

Downstream 100 bases:

>100_bases
CGCCGGCCGGCACGCACGCGCGCCGCCCGCCGGCGACGATGACGAGGCGAAACCCGCGGGTTTCGCCTCTTTTTTTGCGC
GAGCGGACATGAAGGGCAAC

Product: methylenetetrahydrofolate reductase (NAD(P)H)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKTVCERLGL

Sequences:

>Translated_276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKTVCERLGL
>Mature_276_residues
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKAGLEAAPHLSCIGSSKDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAG
ANAAITQYFYNADAYFRFVDDAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDKDSIRAFGADV
VTGLCRRLIDAGVPGLHFYTLNGAAATKTVCERLGL

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=38.9285714285714, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=45.985401459854, Blast_Score=250, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=33.6879432624113, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=33.6879432624113, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321313, Length=281, Percent_Identity=35.2313167259786, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=32.5174825174825, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24662658, Length=263, Percent_Identity=26.615969581749, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30173; Mature: 30173

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGLEAAPHLSCIGSSKDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
IRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAGANAAITQYFYNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDKDSIRAFGADV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VTGLCRRLIDAGVPGLHFYTLNGAAATKTVCERLGL
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPIELSFEFFPPKTAEGVDKLRATRAQLAPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
AGLEAAPHLSCIGSSKDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHH
IRAEHGDWFRIEVAAYPEYHPQARSPKADLENFARKVKAGANAAITQYFYNADAYFRFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DAAKLGVDVPVVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDKDSIRAFGADV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
VTGLCRRLIDAGVPGLHFYTLNGAAATKTVCERLGL
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]