| Definition | Burkholderia pseudomallei MSHR346 chromosome I, complete genome. |
|---|---|
| Accession | NC_012695 |
| Length | 4,098,576 |
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The map label for this gene is mrcA [H]
Identifier: 237814104
GI number: 237814104
Start: 3742011
End: 3744332
Strand: Direct
Name: mrcA [H]
Synonym: GBP346_A3890
Alternate gene names: 237814104
Gene position: 3742011-3744332 (Clockwise)
Preceding gene: 237814091
Following gene: 237814106
Centisome position: 91.3
GC content: 66.37
Gene sequence:
>2322_bases GTGCTCGGCGTGGCAGGCGTGTGCGTGGCGCTCGCGGTCAGCATCGGGCTCGTGCTCGGCTACGCGCTCGTCGTCGCGTC GCCGAACATGCCGTCGCTCGACGCGCTGACCGACTACCGCCCGAAGGTGCCGCTGCGCATCTATACGGCGGATCACGTGC TGATCGGCGAATTCGGCGAGGAGCGCCGGGACATCGTCCGCCTGCAGGACGTGCCCGATTCACTGAAGAAAGCGGTGCTC GCGATCGAGGACGCGCGGTTCTACGAACACGGCGGCGTCGATCTGACGGGCATCATCCGCGCCGGCATCGTCGCGCTGAC GAACGGCCACGCGACGCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAATTTCTTCCTGTCGAGCGAGAAAACGT ACACGCGCAAGATCTACGAGATGCTGCTCGCGTACCGAATCGAGCGCGCGCTCACGAAAGATCAGATTCTCGAGGTCTAC ATGAATCAGATCTATCTGGGCCAGCGCGCCTACGGCTTCGCGGCCGCCGCGCACGCGTACTTCAACAAGGATCTGAAGGA CATCACGCTCGCCGAAGCCGCGATGCTCGCGGGCCTGCCGAAAGCGCCGTCCGCGTACAACCCGGTCGCCAATCCGAAGC GCGCGAAGGTCCGGCAGCAATACATCCTGCAGCGGATGCTCGAGCTGAATTTCATCACGCGCGAGCAGTACGACGAGGCG CTCGCCGAGCCGCTCGTCGTCAAGAGTCCGAGCCGGGAATACAGCGTGCACGCGGAGTACGTCGCCGAAATGGTGCGGCA GATGATGTATGCGCAGTACAAGGACGAGGCGTACACGCGCGGGCTGAATGTCGTCACGACGATCGATTCCGCCGACCAGA CGCTCGCGTACCGCGCGCTGCGCAAGGGCCTGATGACGTACGACAGGCGCCACGGCTATCGCGGCCCGGAAGGTTTCATC GAGCTGCCCGCGAACGCGGACGACCGCGAGCAGGCGATCGACGACGCGCTCGTCGATCATCCGGACAACGGCGAATTGAT CGCGGCCGTCGTCACCGAGGCGAGCGCGCGCCAGATTCGCGTCGTATTCCCGAACGGTAGCGACGCCACGGTGAGCGGCG ACGATCTGCGGTTCGCCGCGAGTTCGCTGTCGTCGAACGCGCAGCCGAACAAGCGGATCCGCCCCGGCGCGATCGTGCGC GTGTTCAAGAACGACGACGGCTCGTGGGCGATCGGGCAACTGCCGCAGATCGAGGGCGCATTCATTTCGATCGTCCCGCA GGACGGCGCGATCCGCTCGCTGATCGGCGGCTTCGACTTCAACAAGAACAAGTTCAACCACGTGACGCAGGCGTGGCGGC AGCCCGGCTCGTCGTTCAAGCCGTTCATCTATTCGGCGTCGCTCGACAAGGGGCTCGGCCCCGCGACGATCATCAACGAC GGCCCGCTCTACTTCAGCGCCGCCGAAACGGGCGGCCAGCCGTGGGAGCCGAAAAACTACGGCGGCGGCTTCGAGGGCCC GATGTCGATGCGCGTCGCGCTGCAGCGTTCGCGCAACCTGGTGTCGATCCGCATCCTGAACCACATCGGCCCGAAATACG CGCAGCAATACATCACACGCTTCGGCTTCGACGCGGATCGCCACCCGGCCTATCTGCCGATGGCGCTCGGCGCGGGCCTC GTCACGCCGCTGCAGATGGCGGGCGCGTTCTCGGTGTTCGCGAACGGCGGCTATCGGATCAATCCGTATCTGATCGCCGA AGTGACCGACCCGAGCGGCAACGTCGTCGCGCGCGCGCAGCCGCTCGTCGCCGAGCAGAATGCGCCGCGCGCGATCGACC CGCGCAACGCATACGTGATGAACAGCCTGCTGCAGAGCGTCGCGCAGCGCGGCACGGGCGCGCGCTCGAACGTGCTCAAA CGCACCGATCTCGCCGGCAAGACGGGCACGACGAACGATTCGCGCGACGCGTGGTTCGGCGGCTATCAGCACACGCTCGC GGCGATCGCGTGGATCGGCTACGACAGCCCGCGCAGCCTCGGCGACCGCGAAACCGGCGGCGGCCTCGCGCTGCCCGTGT GGATCGACTACATGGGCCCCGCGCTCAAGAACGTGCCGGAATACAAGATGCCGATGCCCGACGGTGTCACGACGCTCGGC GGCGAACTGTACTTCGACCAGTTCATGCCGGGCCGCGGTTTCGTCGCGACGGTCGGCGTGAATCTGGCGGCCGCGCCGCC CAACTCGGCAAGCGACGTGCCCGGGCACGTCGACGAGCAGGAAAAGCAGGACATCATGAACCTGTTTCGCGGCGGCAACT GA
Upstream 100 bases:
>100_bases ATCGCGGGACTGTTTTTCTGGTGTGTCTATGCAATCCACGAATCCTACGTCCCCGCCTCCTCCGCCCGAGCCGCGCCGCC GCCCGCTGTGGCTGAAGCTC
Downstream 100 bases:
>100_bases GCGCCCCGTACGCGGAAAGACAAAAGCGGCCGAAAGGCCGCTTTTGTCATAGGGAGCTCGGCTGGGCGGCGCCCGCGGCG CGCTGCGCGCGAGCGCCGCC
Product: penicillin-binding protein 1A
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 773; Mature: 773
Protein sequence:
>773_residues MLGVAGVCVALAVSIGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRDIVRLQDVPDSLKKAVL AIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQILEVY MNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAKVRQQYILQRMLELNFITREQYDEA LAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLAYRALRKGLMTYDRRHGYRGPEGFI ELPANADDREQAIDDALVDHPDNGELIAAVVTEASARQIRVVFPNGSDATVSGDDLRFAASSLSSNAQPNKRIRPGAIVR VFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATIIND GPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQYITRFGFDADRHPAYLPMALGAGL VTPLQMAGAFSVFANGGYRINPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDPRNAYVMNSLLQSVAQRGTGARSNVLK RTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDSPRSLGDRETGGGLALPVWIDYMGPALKNVPEYKMPMPDGVTTLG GELYFDQFMPGRGFVATVGVNLAAAPPNSASDVPGHVDEQEKQDIMNLFRGGN
Sequences:
>Translated_773_residues MLGVAGVCVALAVSIGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRDIVRLQDVPDSLKKAVL AIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQILEVY MNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAKVRQQYILQRMLELNFITREQYDEA LAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLAYRALRKGLMTYDRRHGYRGPEGFI ELPANADDREQAIDDALVDHPDNGELIAAVVTEASARQIRVVFPNGSDATVSGDDLRFAASSLSSNAQPNKRIRPGAIVR VFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATIIND GPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQYITRFGFDADRHPAYLPMALGAGL VTPLQMAGAFSVFANGGYRINPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDPRNAYVMNSLLQSVAQRGTGARSNVLK RTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDSPRSLGDRETGGGLALPVWIDYMGPALKNVPEYKMPMPDGVTTLG GELYFDQFMPGRGFVATVGVNLAAAPPNSASDVPGHVDEQEKQDIMNLFRGGN >Mature_773_residues MLGVAGVCVALAVSIGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRDIVRLQDVPDSLKKAVL AIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQILEVY MNQIYLGQRAYGFAAAAHAYFNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAKVRQQYILQRMLELNFITREQYDEA LAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLAYRALRKGLMTYDRRHGYRGPEGFI ELPANADDREQAIDDALVDHPDNGELIAAVVTEASARQIRVVFPNGSDATVSGDDLRFAASSLSSNAQPNKRIRPGAIVR VFKNDDGSWAIGQLPQIEGAFISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATIIND GPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQYITRFGFDADRHPAYLPMALGAGL VTPLQMAGAFSVFANGGYRINPYLIAEVTDPSGNVVARAQPLVAEQNAPRAIDPRNAYVMNSLLQSVAQRGTGARSNVLK RTDLAGKTGTTNDSRDAWFGGYQHTLAAIAWIGYDSPRSLGDRETGGGLALPVWIDYMGPALKNVPEYKMPMPDGVTTLG GELYFDQFMPGRGFVATVGVNLAAAPPNSASDVPGHVDEQEKQDIMNLFRGGN
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=798, Percent_Identity=38.2205513784461, Blast_Score=519, Evalue=1e-148, Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=38.1526104417671, Blast_Score=149, Evalue=9e-37, Organism=Escherichia coli, GI1788867, Length=228, Percent_Identity=32.8947368421053, Blast_Score=120, Evalue=4e-28, Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=34.2696629213483, Blast_Score=98, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 84467; Mature: 84467
Theoretical pI: Translated: 7.18; Mature: 7.18
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLGVAGVCVALAVSIGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIGEFGE CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEECCEEEECCCCH ERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITM HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEECCCCCCCCHHHHH QVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFAAAAHAY HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH FNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAKVRQQYILQRMLELNFITREQYDEA HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH LAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLAYRAL HHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH RKGLMTYDRRHGYRGPEGFIELPANADDREQAIDDALVDHPDNGELIAAVVTEASARQIR HHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEE VVFPNGSDATVSGDDLRFAASSLSSNAQPNKRIRPGAIVRVFKNDDGSWAIGQLPQIEGA EEECCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCC FISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATIIND EEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECC GPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQYITR CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHH FGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANGGYRINPYLIAEVTDPSGNVVARAQ HCCCCCCCCCEEHHHHCCCHHHHHHHHHHHEEEECCCEEECCEEEEEEECCCCCEEEECC PLVAEQNAPRAIDPRNAYVMNSLLQSVAQRGTGARSNVLKRTDLAGKTGTTNDSRDAWFG CHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC GYQHTLAAIAWIGYDSPRSLGDRETGGGLALPVWIDYMGPALKNVPEYKMPMPDGVTTLG CHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHCHHHHCCCCCCCCCCCCCHHCC GELYFDQFMPGRGFVATVGVNLAAAPPNSASDVPGHVDEQEKQDIMNLFRGGN CHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC >Mature Secondary Structure MLGVAGVCVALAVSIGLVLGYALVVASPNMPSLDALTDYRPKVPLRIYTADHVLIGEFGE CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEECCEEEECCCCH ERRDIVRLQDVPDSLKKAVLAIEDARFYEHGGVDLTGIIRAGIVALTNGHATQGASTITM HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEECCCCCCCCHHHHH QVARNFFLSSEKTYTRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFAAAAHAY HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH FNKDLKDITLAEAAMLAGLPKAPSAYNPVANPKRAKVRQQYILQRMLELNFITREQYDEA HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH LAEPLVVKSPSREYSVHAEYVAEMVRQMMYAQYKDEAYTRGLNVVTTIDSADQTLAYRAL HHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH RKGLMTYDRRHGYRGPEGFIELPANADDREQAIDDALVDHPDNGELIAAVVTEASARQIR HHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEE VVFPNGSDATVSGDDLRFAASSLSSNAQPNKRIRPGAIVRVFKNDDGSWAIGQLPQIEGA EEECCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCC FISIVPQDGAIRSLIGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSASLDKGLGPATIIND EEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECC GPLYFSAAETGGQPWEPKNYGGGFEGPMSMRVALQRSRNLVSIRILNHIGPKYAQQYITR CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCHHHHHHHHHH FGFDADRHPAYLPMALGAGLVTPLQMAGAFSVFANGGYRINPYLIAEVTDPSGNVVARAQ HCCCCCCCCCEEHHHHCCCHHHHHHHHHHHEEEECCCEEECCEEEEEEECCCCCEEEECC PLVAEQNAPRAIDPRNAYVMNSLLQSVAQRGTGARSNVLKRTDLAGKTGTTNDSRDAWFG CHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC GYQHTLAAIAWIGYDSPRSLGDRETGGGLALPVWIDYMGPALKNVPEYKMPMPDGVTTLG CHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHCHHHHCCCCCCCCCCCCCHHCC GELYFDQFMPGRGFVATVGVNLAAAPPNSASDVPGHVDEQEKQDIMNLFRGGN CHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA