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The map label for this gene is yaaJ [C]

Identifier: 237814087

GI number: 237814087

Start: 3719092

End: 3720603

Strand: Reverse

Name: yaaJ [C]

Synonym: GBP346_A3873

Alternate gene names: 237814087

Gene position: 3720603-3719092 (Counterclockwise)

Preceding gene: 237814088

Following gene: 237814086

Centisome position: 90.78

GC content: 68.32

Gene sequence:

>1512_bases
ATGGAAGGCTTTGTGCATGCGTTGATCGACGGGATCAACGGCATTCTCTGGAATTACGTGCTGATTGCGCTGCTGCTCGG
CGCGGGCGCGTGGTTCACGCTGCGCTTCAGGATGATTCAGCTGCGCGCGCTGTTCCTCAGCATGCGCCTCGTCGGCAGCA
AGGGCGAGCCGGGCAGCATCTCGTCGTTCCAGGCGTTCGCGACCGGGCTCGCGAGCCGCGTCGGCACGGGCAACATCGCG
GGCGTCGCGGTTGCGATGACGGTGGGCGGGCCGGGCGCGATCTTCTGGATGTGGATGACGGCGCTCGTCGGGATGTCGTC
CGCGTTCGTCGAGGCGACGCTCGCGCAAATCTTCAAGGTGTCGCATCATGACGGCACGTATCGCGGCGGCCCCGCGTATT
ACATCCAGATCGGGCTGCGCTCGCGCGGTTTCGGCGTGCTGTTCTCGCTGTCGTTGATCCTCGCGTTCGGCTTCGTGTTC
AACGCGGTGCAGGCGAACGCGATCGCCGAGGCGTTCAACACGTCGTTCGGCGTGAGCCGCGCCGCCGTCGGGCTCGCACT
CGTCGCGCTGACGGCGCCGATCATCTTCGGCGGGATTCGGCGGATCGCGCACGTCGCGCAGGTGATCGTGCCCGTGATGG
CGATCGGCTATCTCGCGCTCGCGGTCTATGCGGTCGCGACGCACGTCGCGCTCGTGCCGGACATGATCGTGCTGATCGTG
AAGAGCGCGTTCGGCCTCGAGCAAGCGGCGGGCGGCCTGTCCGGCTACGCGGTGAGCCAGGCGGTGTCGATCGGCGTGAA
ACGCGGCCTGTTCTCGAACGAGGCCGGCATGGGCAGCGCGCCGAACGCGGCCGCGACCGCGAGCACGCGGCATCCGGTCA
CGCAGGGGCTGATCCAGATGCTCGGCGTGTTCGTCGACACGATCGTGATCTGCAGCGCGACCGCGTTCGTGATCCTGTTG
TCGGGGCAGTACGAGCCGGGCACGTCGATGGCCGGCGCGGCGCTCACGCAGCGCGCGATCTCGAGCCACGTCGGCGACTG
GGGAGGCATCTACATGGCGGTGGCGATCTTCTTCCTCGCGTTTTCTTCGGTGATCGGCAACTACGCGTATGCGGAAGGCA
ACGTCGGCTTCGTCACGAGCCGGCGCGGCGCGTTGCTGGTGTTCCGGCTCGCGGTGCTCGGCATGGTGATGTTCGGCAGC
GTCGGGCAACTGCCGCTCGTGTGGGCGATGGCCGATACCAGCATGGGGCTGATGGCGCTCATCAATCTGGTCGCGATCCT
GCTGCTTGGCAAGTACGCGCTCGCCGCATGGCGCGATTATCAGCGCCAGCGCGCAGCGGGCGTGGCCGACCCGGTGTTTA
CCCGCCAGACGATTCCCGCGCTCGCGAAGGTGCTCCCGGACGACGTGTGGGGCGAGCACGGGCCGTTGCCGCAAGGCGAC
AAGCTCGCGGCGGGGCGCGCCTCGGGTGCCGACGCGGCACGGGCGGCGGGGCCGGTCGGCTCCGCGCGATGA

Upstream 100 bases:

>100_bases
CATTTTGTAAGAGTCGGTGCAACCTGTCATATCATGTCGACCCTCGGCCGGCTATGACCGGAATCAATACATAATCCGAC
CCCAAATCAGGAGATGATGG

Downstream 100 bases:

>100_bases
CGATGGCGGGCGACCGGTTGCGCTGCTTCGTCGCGCTGACGCTCGATCGGGCGTCGCGCGACGCGCTGGCCGCGCTGCCC
GTCGCCGCCGGCGCGCGGCG

Product: amino-acid carrier protein AlsT

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MEGFVHALIDGINGILWNYVLIALLLGAGAWFTLRFRMIQLRALFLSMRLVGSKGEPGSISSFQAFATGLASRVGTGNIA
GVAVAMTVGGPGAIFWMWMTALVGMSSAFVEATLAQIFKVSHHDGTYRGGPAYYIQIGLRSRGFGVLFSLSLILAFGFVF
NAVQANAIAEAFNTSFGVSRAAVGLALVALTAPIIFGGIRRIAHVAQVIVPVMAIGYLALAVYAVATHVALVPDMIVLIV
KSAFGLEQAAGGLSGYAVSQAVSIGVKRGLFSNEAGMGSAPNAAATASTRHPVTQGLIQMLGVFVDTIVICSATAFVILL
SGQYEPGTSMAGAALTQRAISSHVGDWGGIYMAVAIFFLAFSSVIGNYAYAEGNVGFVTSRRGALLVFRLAVLGMVMFGS
VGQLPLVWAMADTSMGLMALINLVAILLLGKYALAAWRDYQRQRAAGVADPVFTRQTIPALAKVLPDDVWGEHGPLPQGD
KLAAGRASGADAARAAGPVGSAR

Sequences:

>Translated_503_residues
MEGFVHALIDGINGILWNYVLIALLLGAGAWFTLRFRMIQLRALFLSMRLVGSKGEPGSISSFQAFATGLASRVGTGNIA
GVAVAMTVGGPGAIFWMWMTALVGMSSAFVEATLAQIFKVSHHDGTYRGGPAYYIQIGLRSRGFGVLFSLSLILAFGFVF
NAVQANAIAEAFNTSFGVSRAAVGLALVALTAPIIFGGIRRIAHVAQVIVPVMAIGYLALAVYAVATHVALVPDMIVLIV
KSAFGLEQAAGGLSGYAVSQAVSIGVKRGLFSNEAGMGSAPNAAATASTRHPVTQGLIQMLGVFVDTIVICSATAFVILL
SGQYEPGTSMAGAALTQRAISSHVGDWGGIYMAVAIFFLAFSSVIGNYAYAEGNVGFVTSRRGALLVFRLAVLGMVMFGS
VGQLPLVWAMADTSMGLMALINLVAILLLGKYALAAWRDYQRQRAAGVADPVFTRQTIPALAKVLPDDVWGEHGPLPQGD
KLAAGRASGADAARAAGPVGSAR
>Mature_503_residues
MEGFVHALIDGINGILWNYVLIALLLGAGAWFTLRFRMIQLRALFLSMRLVGSKGEPGSISSFQAFATGLASRVGTGNIA
GVAVAMTVGGPGAIFWMWMTALVGMSSAFVEATLAQIFKVSHHDGTYRGGPAYYIQIGLRSRGFGVLFSLSLILAFGFVF
NAVQANAIAEAFNTSFGVSRAAVGLALVALTAPIIFGGIRRIAHVAQVIVPVMAIGYLALAVYAVATHVALVPDMIVLIV
KSAFGLEQAAGGLSGYAVSQAVSIGVKRGLFSNEAGMGSAPNAAATASTRHPVTQGLIQMLGVFVDTIVICSATAFVILL
SGQYEPGTSMAGAALTQRAISSHVGDWGGIYMAVAIFFLAFSSVIGNYAYAEGNVGFVTSRRGALLVFRLAVLGMVMFGS
VGQLPLVWAMADTSMGLMALINLVAILLLGKYALAAWRDYQRQRAAGVADPVFTRQTIPALAKVLPDDVWGEHGPLPQGD
KLAAGRASGADAARAAGPVGSAR

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=470, Percent_Identity=41.7021276595745, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52298; Mature: 52298

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGFVHALIDGINGILWNYVLIALLLGAGAWFTLRFRMIQLRALFLSMRLVGSKGEPGSI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SSFQAFATGLASRVGTGNIAGVAVAMTVGGPGAIFWMWMTALVGMSSAFVEATLAQIFKV
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SHHDGTYRGGPAYYIQIGLRSRGFGVLFSLSLILAFGFVFNAVQANAIAEAFNTSFGVSR
HCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AAVGLALVALTAPIIFGGIRRIAHVAQVIVPVMAIGYLALAVYAVATHVALVPDMIVLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAFGLEQAAGGLSGYAVSQAVSIGVKRGLFSNEAGMGSAPNAAATASTRHPVTQGLIQM
HHHHCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LGVFVDTIVICSATAFVILLSGQYEPGTSMAGAALTQRAISSHVGDWGGIYMAVAIFFLA
HHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FSSVIGNYAYAEGNVGFVTSRRGALLVFRLAVLGMVMFGSVGQLPLVWAMADTSMGLMAL
HHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
INLVAILLLGKYALAAWRDYQRQRAAGVADPVFTRQTIPALAKVLPDDVWGEHGPLPQGD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
KLAAGRASGADAARAAGPVGSAR
CCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MEGFVHALIDGINGILWNYVLIALLLGAGAWFTLRFRMIQLRALFLSMRLVGSKGEPGSI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SSFQAFATGLASRVGTGNIAGVAVAMTVGGPGAIFWMWMTALVGMSSAFVEATLAQIFKV
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SHHDGTYRGGPAYYIQIGLRSRGFGVLFSLSLILAFGFVFNAVQANAIAEAFNTSFGVSR
HCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AAVGLALVALTAPIIFGGIRRIAHVAQVIVPVMAIGYLALAVYAVATHVALVPDMIVLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAFGLEQAAGGLSGYAVSQAVSIGVKRGLFSNEAGMGSAPNAAATASTRHPVTQGLIQM
HHHHCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LGVFVDTIVICSATAFVILLSGQYEPGTSMAGAALTQRAISSHVGDWGGIYMAVAIFFLA
HHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FSSVIGNYAYAEGNVGFVTSRRGALLVFRLAVLGMVMFGSVGQLPLVWAMADTSMGLMAL
HHHHHCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
INLVAILLLGKYALAAWRDYQRQRAAGVADPVFTRQTIPALAKVLPDDVWGEHGPLPQGD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
KLAAGRASGADAARAAGPVGSAR
CCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]