Click here to switch to the map view.

The map label for this gene is araF [H]

Identifier: 237813852

GI number: 237813852

Start: 3504581

End: 3505576

Strand: Reverse

Name: araF [H]

Synonym: GBP346_A3630

Alternate gene names: 237813852

Gene position: 3505576-3504581 (Counterclockwise)

Preceding gene: 237813853

Following gene: 237813851

Centisome position: 85.53

GC content: 67.47

Gene sequence:

>996_bases
ATGAAACGCAGAACGTTCATCACGCTGGCGGCAGCGGCGACGGTCGCGGCGGCGGGCCTGCCCGCGCAGGCGGCCGAGCC
CGTGAAGATCGGCTTCCTCGTCAAGCAGCCCGAGGAGCCGTGGTTCCAGGACGAATGGAAATTCGCCGAGCTCGCCGCGA
AGGACAAGGGCTTCACGCTCGTGAAGATCGGCGCGCCGTCCGGCGAGAAGGTGATGAGCGCGATCGACAATCTCGCCGCG
CAGAAGGCGCAGGGCTTCATCATCTGCACGCCGGACGTGAAGCTCGGGCCGGGCATCGTCGCGAAGGCGAAGTCGCACGG
CCTGAAGATGATGACGGTCGATGACCGGCTCGTCGACGGCGCGGGCAAGCCGATCGAATCGGTTCCGCACATGGGCATTT
CCGCGTACGACATCGGCAAGCAGGTCGGCGGCGGGATCGCGGCCGAGATCAAGAGGCGCGGCTGGAACATGAACGAAGTC
GGCGCGATCGACATCACGTACGAGCAGTTGCCGACCGCGCACGACCGCACGACGGGCGCGACCGACGCGCTCGTCGCCGC
GGGCTTTCCGAAGGCGAACGTGATCGCCGCGCCGCAGGCGAAGACCGACACCGAGAACGCGTTCAACGCGGCGAACATCG
CGCTCACGAAGAATCCGAAGTTCAAGCACTGGGTCGCCTACGGCCTGAACGACGAAGCGGTGCTCGGCGCGGTGCGCGCG
GCCGAAGGGCGCGGCTTCAAGGCGGCCGACATGATCGGCATCGGCATCGGCGGCTCGGACTCGGCGCTCAGCGAGTTCAA
GAAGCCGCAGCCGACCGGCTTCTTCGGCACCGTGATCATCAGCCCGAAGCGGCACGGCGAAGAGACTTCCGAGCTGATGT
ACGCGTGGATCACGCAAGGCAAGGCGCCGCCGCCGCTCACGCTGACGACGGGCATGCTCGCGACGCGCGAGAACGTCGCG
CAGGTGCGCGAGACGATGGGGCTCGCGGCGAAGTGA

Upstream 100 bases:

>100_bases
GTGCACGCGCGAAGGCGGCGCGCGACGGTCGCACGCGCCGCGTCGAAGCGACGAAGCGGCCGACGGATCCGGACACGGAG
ACAACAAGGAGACAAGCAAC

Downstream 100 bases:

>100_bases
GCGAAGTCCGCCGCGCGTCGCGCGCTGCGCGGCGCGCGGCGATGCGGCAGGCAATGAGGCAACGAGCATGGCAGGAAACG
GAGGCGACGTGGCTGCGGCA

Product: L-arabinose-binding periplasmic protein (ABP)

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKRRTFITLAAAATVAAAGLPAQAAEPVKIGFLVKQPEEPWFQDEWKFAELAAKDKGFTLVKIGAPSGEKVMSAIDNLAA
QKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKRRGWNMNEV
GAIDITYEQLPTAHDRTTGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLGAVRA
AEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITQGKAPPPLTLTTGMLATRENVA
QVRETMGLAAK

Sequences:

>Translated_331_residues
MKRRTFITLAAAATVAAAGLPAQAAEPVKIGFLVKQPEEPWFQDEWKFAELAAKDKGFTLVKIGAPSGEKVMSAIDNLAA
QKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKRRGWNMNEV
GAIDITYEQLPTAHDRTTGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLGAVRA
AEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITQGKAPPPLTLTTGMLATRENVA
QVRETMGLAAK
>Mature_331_residues
MKRRTFITLAAAATVAAAGLPAQAAEPVKIGFLVKQPEEPWFQDEWKFAELAAKDKGFTLVKIGAPSGEKVMSAIDNLAA
QKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKRRGWNMNEV
GAIDITYEQLPTAHDRTTGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLGAVRA
AEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITQGKAPPPLTLTTGMLATRENVA
QVRETMGLAAK

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=327, Percent_Identity=48.9296636085627, Blast_Score=320, Evalue=6e-89,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34938; Mature: 34938

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFITLAAAATVAAAGLPAQAAEPVKIGFLVKQPEEPWFQDEWKFAELAAKDKGFTL
CCCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEE
VKIGAPSGEKVMSAIDNLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDG
EEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEHHCCCEEEEEECCHHHCC
AGKPIESVPHMGISAYDIGKQVGGGIAAEIKRRGWNMNEVGAIDITYEQLPTAHDRTTGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITQG
CCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCC
KAPPPLTLTTGMLATRENVAQVRETMGLAAK
CCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRRTFITLAAAATVAAAGLPAQAAEPVKIGFLVKQPEEPWFQDEWKFAELAAKDKGFTL
CCCEEEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEE
VKIGAPSGEKVMSAIDNLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDG
EEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEHHCCCEEEEEECCHHHCC
AGKPIESVPHMGISAYDIGKQVGGGIAAEIKRRGWNMNEVGAIDITYEQLPTAHDRTTGA
CCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCCC
TDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLGAVRA
CHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHH
AEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITQG
CCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCC
KAPPPLTLTTGMLATRENVAQVRETMGLAAK
CCCCCEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]